##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938555_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2332346 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.120156271839598 31.0 31.0 34.0 28.0 34.0 2 31.138099149954595 31.0 31.0 34.0 27.0 34.0 3 31.186142193310943 31.0 31.0 34.0 27.0 34.0 4 34.847155181949844 37.0 35.0 37.0 32.0 37.0 5 34.82289891808505 37.0 35.0 37.0 32.0 37.0 6 34.8495364752914 37.0 35.0 37.0 32.0 37.0 7 34.75276181149795 37.0 35.0 37.0 31.0 37.0 8 34.716362838103784 37.0 35.0 37.0 31.0 37.0 9 36.21060725981479 39.0 35.0 39.0 32.0 39.0 10-11 36.044026915389054 39.0 35.0 39.0 30.0 39.0 12-13 35.89893716455449 38.0 35.0 39.0 30.0 39.0 14-15 36.86178551552814 39.0 36.0 41.0 30.5 41.0 16-17 36.69013495424778 39.0 36.0 41.0 30.0 41.0 18-19 36.83932529736154 39.0 36.0 41.0 30.0 41.0 20-21 36.8088544752794 39.0 36.0 41.0 30.0 41.0 22-23 36.73170404391115 39.0 36.0 41.0 30.0 41.0 24-25 36.58758799080411 39.0 36.0 41.0 30.0 41.0 26-27 36.37174415802801 39.0 35.5 41.0 29.5 41.0 28-29 36.16706890830091 39.0 35.0 40.0 29.0 41.0 30-31 35.91433668074977 38.5 35.0 40.0 27.5 41.0 32-33 35.645412172979476 38.0 34.5 40.0 27.0 41.0 34-35 35.389314021161525 38.0 34.0 40.0 26.5 41.0 36-37 35.11593391375037 38.0 34.0 40.0 25.5 41.0 38-39 34.985683727886 38.0 34.0 40.0 25.0 41.0 40-41 34.88747081264958 38.0 34.0 40.0 25.0 41.0 42-43 34.58077939550993 38.0 33.0 40.0 24.0 41.0 44-45 34.24240614385687 38.0 33.0 40.0 23.5 41.0 46-47 33.84939412934445 38.0 33.0 40.0 22.5 41.0 48-49 33.47848518187267 37.5 32.5 40.0 20.0 41.0 50-51 32.7579602254554 36.5 31.5 39.5 18.5 40.5 52-53 32.766989546147954 37.0 31.5 39.0 18.0 40.0 54-55 32.86897462897872 37.0 31.5 40.0 17.5 41.0 56-57 32.53883021644302 36.5 31.0 39.5 14.0 41.0 58-59 32.158553019148954 36.0 31.0 39.0 11.0 41.0 60-61 31.672824057837047 35.5 30.5 39.0 8.0 40.0 62-63 31.105928108436743 35.0 29.5 39.0 7.0 40.0 64-65 30.58664387702339 35.0 29.0 38.0 2.0 40.0 66-67 30.04599553411029 34.5 28.0 38.0 2.0 40.0 68-69 29.457443063764984 34.0 28.0 37.0 2.0 39.5 70-71 28.821449947820778 34.0 26.5 36.5 2.0 39.0 72-73 28.195012875448157 33.5 26.0 36.0 2.0 39.0 74-75 27.530368135774022 33.0 25.5 35.5 2.0 38.0 76-77 26.803590033382697 32.5 23.5 35.0 2.0 37.0 78-79 26.031960523867383 32.0 21.5 35.0 2.0 36.5 80-81 25.347891564973636 31.0 19.5 35.0 2.0 36.0 82-83 24.63134522064908 31.0 17.0 34.0 2.0 35.5 84-85 23.95172564447985 30.5 9.5 34.0 2.0 35.0 86-87 23.201700562437992 30.0 4.5 34.0 2.0 35.0 88-89 22.386923938386502 29.5 2.0 34.0 2.0 35.0 90-91 21.476506273082983 29.0 2.0 34.0 2.0 35.0 92-93 20.536125000321565 27.5 2.0 33.5 2.0 35.0 94-95 19.533264790044015 26.5 2.0 33.0 2.0 35.0 96-97 18.275174652474377 24.5 2.0 32.5 2.0 34.0 98-99 16.75366755189839 11.0 2.0 32.0 2.0 34.0 100-101 13.225937532424435 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45636494874104017 1101 2 0.38459873681219037 1101 3 0.4614111404070229 1101 4 0.3947843462113312 1101 5 0.33700033082475045 1101 6 0.5768622720973298 1101 7 0.4890528253488924 1101 8 0.6070703938374962 1101 9 0.7858704748022305 1101 10-11 0.6905367873630723 1101 12-13 0.7902399612103466 1101 14-15 0.9103791058818729 1101 16-17 0.8246380861136302 1101 18-19 0.7265514065588619 1101 20-21 0.835768551395276 1101 22-23 0.896001571875388 1101 24-25 0.8020793887659465 1101 26-27 0.8186960844570379 1101 28-29 0.9633279281368843 1101 30-31 0.9512146642161028 1101 32-33 0.971470341444153 1101 34-35 0.9620238451119292 1101 36-37 0.7946524083225839 1101 38-39 1.0103726625342873 1101 40-41 0.9686647744399011 1101 42-43 1.1773571044227822 1101 44-45 1.149497368186303 1101 46-47 0.9244940319737651 1101 48-49 0.9431493189550935 1101 50-51 1.0439552248433372 1101 52-53 0.9679906675795991 1101 54-55 1.1531363426839079 1101 56-57 1.0857997106749337 1101 58-59 1.1830585027940188 1101 60-61 1.1209468653605406 1101 62-63 1.1200725633591588 1101 64-65 1.1057353427559526 1101 66-67 1.266235591430192 1101 68-69 1.2515455903478419 1101 70-71 1.1851742475911742 1101 72-73 1.3269185147944427 1101 74-75 1.183134680507532 1101 76-77 1.2498303043134342 1101 78-79 1.550022995568316 1101 80-81 1.4219305744712365 1101 82-83 1.3257165979546883 1101 84-85 1.4189699711939028 1101 86-87 1.7014699363573982 1101 88-89 1.7796264946466493 1101 90-91 1.6533505785081317 1101 92-93 1.758185621635274 1101 94-95 1.6145832373401738 1101 96-97 1.1509483429044955 1101 98-99 1.3380351788984584 1101 100-101 1.1579978326629234 1102 1 0.2924589451825703 1102 2 0.20182157628478947 1102 3 0.23376035218260682 1102 4 0.19229574807759064 1102 5 0.19433715011196995 1102 6 0.21269443351823014 1102 7 0.204826248705821 1102 8 0.3674540559537718 1102 9 0.5607183851722937 1102 10-11 0.5296630586614484 1102 12-13 0.42765847768575327 1102 14-15 0.43713347821593374 1102 16-17 0.5920522516213325 1102 18-19 0.3925243140153256 1102 20-21 0.42020218748344007 1102 22-23 0.5154321389774097 1102 24-25 0.4735914277381923 1102 26-27 0.4523010942855592 1102 28-29 0.31521846245125573 1102 30-31 0.4676296610694237 1102 32-33 0.41665922005991973 1102 34-35 0.38900547781256023 1102 36-37 0.6605056641672533 1102 38-39 0.4135667390025404 1102 40-41 0.4082824720010336 1102 42-43 0.5147818365663284 1102 44-45 0.6742155264838274 1102 46-47 0.5735012732271585 1102 48-49 0.6875507501126847 1102 50-51 0.6134081295027514 1102 52-53 0.5318607368066068 1102 54-55 0.5858542601243002 1102 56-57 0.5945142716545178 1102 58-59 0.713557620946105 1102 60-61 0.5102712610810478 1102 62-63 0.5204901785626852 1102 64-65 0.8256369409596225 1102 66-67 0.45798332076892123 1102 68-69 0.6375982137482765 1102 70-71 0.950145072798815 1102 72-73 0.8950577768392414 1102 74-75 0.8197688573588344 1102 76-77 1.029902483759777 1102 78-79 0.8741613485716506 1102 80-81 0.9743636200709318 1102 82-83 1.1223018652672678 1102 84-85 1.0244457805904403 1102 86-87 1.0959750914771647 1102 88-89 1.0971131115361743 1102 90-91 0.979306237485499 1102 92-93 1.0410839876915112 1102 94-95 0.85499692890318 1102 96-97 1.0139639520011237 1102 98-99 0.7665382906173228 1102 100-101 0.6736152815973266 1103 1 0.4524514113098057 1103 2 0.3291940788356271 1103 3 0.4556970468887265 1103 4 0.3776330965103085 1103 5 0.373773829987627 1103 6 0.515018514623037 1103 7 0.41860989901547896 1103 8 0.3435247408836517 1103 9 0.5927206601645878 1103 10-11 0.6982132553133269 1103 12-13 0.717915756474639 1103 14-15 0.8310627043032994 1103 16-17 0.7140032947957593 1103 18-19 0.7892700294959596 1103 20-21 0.7418238461812052 1103 22-23 0.8269040174625673 1103 24-25 0.7599453299283496 1103 26-27 0.8024854739571552 1103 28-29 0.9456504885291324 1103 30-31 0.9019579822022337 1103 32-33 0.9646466898057895 1103 34-35 0.891071546020811 1103 36-37 1.209763177277381 1103 38-39 1.0694337424194842 1103 40-41 1.0990257078290426 1103 42-43 1.147763033028376 1103 44-45 1.0094415352515966 1103 46-47 0.890765557924496 1103 48-49 0.788368390349504 1103 50-51 1.0955947100143462 1103 52-53 1.0237238205334336 1103 54-55 0.9356061465585839 1103 56-57 0.8951791952479979 1103 58-59 0.9074512168576447 1103 60-61 1.0144375075514098 1103 62-63 1.0102363997619364 1103 64-65 1.214736628333565 1103 66-67 1.1394653062113456 1103 68-69 1.2532631818331303 1103 70-71 1.4924467302508262 1103 72-73 1.3703422621036978 1103 74-75 1.3227106641238926 1103 76-77 1.4067249616920527 1103 78-79 1.5725411580892477 1103 80-81 1.6886767177383994 1103 82-83 1.5641994668855688 1103 84-85 1.6833886210384392 1103 86-87 1.7798468198780881 1103 88-89 1.6153136059836513 1103 90-91 1.59852031959878 1103 92-93 1.82265135949703 1103 94-95 2.1810842239462467 1103 96-97 2.331986073112578 1103 98-99 2.149809051775758 1103 100-101 2.165793141799494 1104 1 0.4654242166213187 1104 2 0.31172200844017084 1104 3 0.3986645568925269 1104 4 0.36522579932478294 1104 5 0.42499999411701594 1104 6 0.5162808656837754 1104 7 0.4165878319732528 1104 8 0.5817126418739775 1104 9 0.5753282289616735 1104 10-11 0.6173974635600317 1104 12-13 0.6395465994764464 1104 14-15 0.6716037338564291 1104 16-17 0.8507334841973204 1104 18-19 0.7679454131612573 1104 20-21 0.7334730830421421 1104 22-23 0.9078324567579443 1104 24-25 0.9139325988349682 1104 26-27 0.8023522180275293 1104 28-29 0.9093542279385147 1104 30-31 0.876983236209341 1104 32-33 0.7837845117157869 1104 34-35 0.6937795929324864 1104 36-37 0.826666176740865 1104 38-39 0.9021386945800813 1104 40-41 0.9151072010778734 1104 42-43 0.9933036923291141 1104 44-45 0.8460936922162574 1104 46-47 1.0111653466710564 1104 48-49 0.8037535995351135 1104 50-51 0.931552119812487 1104 52-53 0.8488695113545361 1104 54-55 0.989469485704177 1104 56-57 1.137807330517262 1104 58-59 1.0367567980281436 1104 60-61 0.9467026278205601 1104 62-63 1.1580495354782983 1104 64-65 1.0309688422472298 1104 66-67 1.0304946148313796 1104 68-69 1.1511091512516778 1104 70-71 1.3114806369863459 1104 72-73 1.429504576558351 1104 74-75 1.3744525618839383 1104 76-77 1.2133377741012374 1104 78-79 1.3801896292961864 1104 80-81 1.2706097286028601 1104 82-83 1.2105193677417603 1104 84-85 1.3399624172291666 1104 86-87 1.4760281904546204 1104 88-89 1.4549103173041544 1104 90-91 1.3064905610871698 1104 92-93 1.5417282993400967 1104 94-95 1.7045257408353471 1104 96-97 1.8880136422449851 1104 98-99 2.088438496201576 1104 100-101 1.8774421551118134 1105 1 0.34468555722041927 1105 2 0.26947351268123754 1105 3 0.24734439276401332 1105 4 0.15965868827854734 1105 5 0.26094040833067567 1105 6 0.24593509296608573 1105 7 0.4038129329317499 1105 8 0.21243869159907547 1105 9 0.4983220335968426 1105 10-11 0.5106069909607314 1105 12-13 0.5087267880944921 1105 14-15 0.713981468655895 1105 16-17 0.6459402869349518 1105 18-19 0.5708057011970666 1105 20-21 0.5760528362006383 1105 22-23 0.615382753551593 1105 24-25 0.8322271705925388 1105 26-27 0.6609758577232725 1105 28-29 0.6461313002231819 1105 30-31 0.7743344947441884 1105 32-33 0.6494017573733544 1105 34-35 0.7103186423818784 1105 36-37 0.6961088979981085 1105 38-39 0.6742821570105448 1105 40-41 0.8428803161138063 1105 42-43 0.8213037612566012 1105 44-45 0.844915336258488 1105 46-47 0.9633565312507599 1105 48-49 0.8557386098336863 1105 50-51 0.6877728256299633 1105 52-53 0.8642155070729132 1105 54-55 0.8692199073782234 1105 56-57 0.7454568626212534 1105 58-59 0.7369272921976844 1105 60-61 0.8375777433754656 1105 62-63 0.7800319305868797 1105 64-65 0.9397934866624773 1105 66-67 0.9708845011415654 1105 68-69 0.9650632171625766 1105 70-71 0.9972990090001623 1105 72-73 1.0571425734444517 1105 74-75 1.1953328267276753 1105 76-77 1.315639850845912 1105 78-79 1.3527823367679233 1105 80-81 1.549045358437919 1105 82-83 1.5356792186962025 1105 84-85 1.4948480584169914 1105 86-87 1.6605602539003463 1105 88-89 1.4932269053123903 1105 90-91 1.446312505166702 1105 92-93 1.6798265493928106 1105 94-95 1.8717666342977743 1105 96-97 2.210850231388502 1105 98-99 2.2966512064304148 1105 100-101 2.032144327544062 1106 1 0.28173214456284157 1106 2 0.24075454209980762 1106 3 0.054590420426777087 1106 4 0.1888531892081673 1106 5 0.2722789573557307 1106 6 0.12066389605194416 1106 7 0.38826130411695914 1106 8 0.36100883394172456 1106 9 0.298911316929221 1106 10-11 0.26983661818577076 1106 12-13 0.3507946526552814 1106 14-15 0.4311679180655901 1106 16-17 0.3448008685825883 1106 18-19 0.3089861774692153 1106 20-21 0.2633661969190797 1106 22-23 0.3170381606123769 1106 24-25 0.1514552019411397 1106 26-27 0.1839295970421304 1106 28-29 0.4571854838013749 1106 30-31 0.124579099388761 1106 32-33 0.2207131901097128 1106 34-35 0.0594287796340609 1106 36-37 0.26636174403447654 1106 38-39 0.32724597859360216 1106 40-41 0.4654097700722559 1106 42-43 0.07949623892794477 1106 44-45 0.10270952536270528 1106 46-47 0.31780996228304303 1106 48-49 0.7214766567531399 1106 50-51 0.42032928358730004 1106 52-53 0.458710703110512 1106 54-55 0.6287629252656473 1106 56-57 -0.045497444271575205 1106 58-59 0.24361789360832375 1106 60-61 0.7498449461749495 1106 62-63 0.5357057987736056 1106 64-65 0.4876931737481982 1106 66-67 0.23223325833151875 1106 68-69 0.2904953457963444 1106 70-71 0.1378235861779764 1106 72-73 0.5361298316130281 1106 74-75 0.8056443833231022 1106 76-77 0.847165301162093 1106 78-79 0.9079430915460271 1106 80-81 0.9075127249763746 1106 82-83 0.6719749784967206 1106 84-85 0.6854473423482546 1106 86-87 0.7657201750116478 1106 88-89 0.822700361361477 1106 90-91 0.8949699443328427 1106 92-93 0.6879576930067159 1106 94-95 0.8366526737475688 1106 96-97 0.9524289206873497 1106 98-99 1.1813375639666255 1106 100-101 0.5893526700647431 1107 1 0.19363547171429474 1107 2 0.11115833475821901 1107 3 0.24173964190309505 1107 4 0.15721795690834028 1107 5 0.23837499028770992 1107 6 0.3408527493954452 1107 7 0.31609155405762124 1107 8 0.18511919151708156 1107 9 0.4057206561282882 1107 10-11 0.5611602917314613 1107 12-13 0.524904869243322 1107 14-15 0.5684958699958784 1107 16-17 0.5884337568438269 1107 18-19 0.6754906755452268 1107 20-21 0.6475587982031215 1107 22-23 0.6220131386805576 1107 24-25 0.6771360421459391 1107 26-27 0.5997130610872929 1107 28-29 0.7138758711698401 1107 30-31 0.8267121214220978 1107 32-33 0.8832851703562312 1107 34-35 0.8690928300736829 1107 36-37 0.7963304232868396 1107 38-39 0.8395908282687756 1107 40-41 0.8641851044568511 1107 42-43 0.9385752666329452 1107 44-45 0.8500991451464373 1107 46-47 0.9555255464075998 1107 48-49 0.8680323116632849 1107 50-51 0.9467085086247877 1107 52-53 0.8495894206765939 1107 54-55 0.9750330062139341 1107 56-57 0.8377200223654384 1107 58-59 0.7894066770221073 1107 60-61 0.8231156413303502 1107 62-63 1.0566954932640442 1107 64-65 0.9895663976189759 1107 66-67 1.0719047180694368 1107 68-69 1.2528154895171575 1107 70-71 1.3253350621310922 1107 72-73 1.2369594673465762 1107 74-75 1.2989233519461258 1107 76-77 1.3699486860884917 1107 78-79 1.4442921933987485 1107 80-81 1.5491610259713617 1107 82-83 1.288943532187428 1107 84-85 1.2991803199954575 1107 86-87 1.4599297926357906 1107 88-89 1.293152819525151 1107 90-91 1.255507195587672 1107 92-93 1.2251213698299424 1107 94-95 1.3522986171028428 1107 96-97 1.6203682052447057 1107 98-99 1.727878804996724 1107 100-101 1.7822226593546837 1108 1 0.37542480816965096 1108 2 0.18168515601695034 1108 3 0.26009851155916763 1108 4 0.20696794029386467 1108 5 0.2513722565195522 1108 6 0.30621219418716805 1108 7 0.3328344474346636 1108 8 0.3278792530181249 1108 9 0.27050181726271205 1108 10-11 0.41726395006390504 1108 12-13 0.4553651726411232 1108 14-15 0.5259046015644273 1108 16-17 0.6101230166977132 1108 18-19 0.5838125036671116 1108 20-21 0.3885246463383254 1108 22-23 0.42225118787639815 1108 24-25 0.5651092611117221 1108 26-27 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2316 72-73 -2.3395860754309687 2316 74-75 -2.44112926572598 2316 76-77 -2.5778101323186604 2316 78-79 -2.7003598295986926 2316 80-81 -2.679150895136214 2316 82-83 -2.928303571830341 2316 84-85 -3.023252657496016 2316 86-87 -3.240508015118934 2316 88-89 -3.4454122014866755 2316 90-91 -3.4582805967881107 2316 92-93 -3.5082469073822296 2316 94-95 -3.847440230348095 2316 96-97 -4.039397293532007 2316 98-99 -3.912354356049388 2316 100-101 -3.5868954726302658 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7902.0 3 2767.0 4 3003.0 5 3517.0 6 4299.0 7 5120.0 8 6597.0 9 8215.0 10 10407.0 11 12792.0 12 15508.0 13 18018.0 14 20706.0 15 23527.0 16 26967.0 17 31329.0 18 30695.0 19 29567.0 20 30930.0 21 33931.0 22 37025.0 23 41633.0 24 45909.0 25 51340.0 26 57911.0 27 65280.0 28 74601.0 29 85054.0 30 97955.0 31 114065.0 32 134269.0 33 159720.0 34 193068.0 35 233100.0 36 267176.0 37 244873.0 38 99523.0 39 4047.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45876095266477 17.827325759638452 14.010663773826803 23.70324951386998 2 15.930502964599071 19.476862220612183 39.33259393619953 25.26004087858922 3 19.772858876407774 23.2538923178688 28.717882019339363 28.255366786384066 4 13.132490349122868 15.651312403389278 35.53660508048443 35.67959216700342 5 14.328308855106073 37.06042257150219 33.20686249248476 15.404406080906982 6 33.86112048121416 34.710652648420286 16.760267558643253 14.667959311722301 7 30.411852726121 31.967767488119097 19.226884615104197 18.3934951706557 8 27.70953971040946 33.78633498391307 18.9977638690298 19.506361436647673 9 27.257849740432675 16.352462619517834 19.129371956436433 37.26031568361306 10-11 25.88826847515851 25.891185489739936 26.416548395309526 21.803997639792026 12-13 26.88077273116289 23.08281673996319 26.835492833399794 23.200917695474132 14-15 23.24295021777165 24.58603443732013 25.152019858595853 27.01899548631237 16-17 23.092038248659176 27.92128530591851 25.895495982346773 23.09118046307555 18-19 23.254356642605295 26.11444246405216 28.18935790897454 22.44184298436801 20-21 23.5649223450999 25.36596603793983 28.31709677668457 22.7520148402757 22-23 23.298714388342646 25.29451122095579 28.29876800017156 23.108006390530004 24-25 23.192060440243964 25.554809146683073 28.278622420921412 22.974507992151555 26-27 22.528484690403943 25.98585836312398 28.181364219221972 23.304292727250107 28-29 22.726668248191935 26.483527184574722 28.03739720979476 22.75240735743858 30-31 22.716164272361684 25.807201063696965 27.89248388601212 23.58415077792923 32-33 22.40785071914092 25.93417968259865 27.935702965425424 23.722266632835005 34-35 23.488027327129497 26.001230012563976 27.627060362212312 22.88368229809421 36-37 23.564448263340225 25.397318045548054 27.66672021795733 23.371513473154394 38-39 22.78119331244769 25.891866285838542 27.79677176753758 23.530168634176185 40-41 23.295211821443093 25.70238285183908 27.879186375076557 23.123218951641267 42-43 22.986939796799348 25.675023063690094 27.62845649765589 23.709580641854668 44-45 22.907259293816185 26.018633298015708 27.818224150994205 23.255883257173902 46-47 23.460100706359594 26.390855167874307 26.989754623391022 23.15928950237508 48-49 23.17609973223985 26.32910464256947 27.010843546302954 23.483952078887725 50-51 23.300004845337263 26.524533915002497 27.080632843925684 23.09482839573456 52-53 23.70199535357988 26.156888287813914 26.963193972174647 23.17792238643156 54-55 22.86399198319627 26.370657537847304 27.32314609805791 23.442204380898517 56-57 23.076908233400278 26.661869679906193 27.02541724029323 23.2358048464003 58-59 23.10816197311598 25.91402181606416 27.05585625146474 23.921959959355128 60-61 22.611459368402272 26.33684983375283 27.570698469702613 23.480992328142282 62-63 22.830738713559423 26.27649354026829 27.60312962364631 23.28963812252597 64-65 22.42003533665117 26.37093535785488 27.361699418066543 23.847329887427403 66-67 22.485742586144795 26.44934651733187 27.26161175239696 23.803299144126374 68-69 23.0515429171262 25.84521259169941 27.603560089808393 23.499684401365993 70-71 22.836531914870804 26.174129070511754 27.167295875314828 23.82204313930261 72-73 22.903627148330784 26.14798511296937 27.128045103688564 23.820342635011283 74-75 22.74654781063807 26.70630202140309 27.00465009558589 23.542500072372942 76-77 22.987975843616493 26.173725429988604 27.080147319288137 23.758151407106766 78-79 23.07126657180779 26.437670450659702 27.033021736860736 23.458041240671776 80-81 23.164605239547242 26.55996254250467 26.81521033288334 23.460221885064744 82-83 23.08552172758139 26.52985357012463 26.859326685414274 23.52529801687971 84-85 22.96308714832308 26.52503294584404 26.908184483005904 23.603695422826974 86-87 23.213123344273598 26.555257202007844 26.596336797798344 23.63528265592022 88-89 23.058392241418137 26.613250855580866 26.43664455316997 23.891712349831028 90-91 22.95524408399181 26.594919264872207 26.62429932837604 23.825537322759942 92-93 23.244715925722208 26.04845704923868 26.86212889785249 23.84469812718662 94-95 22.94884190586657 25.985332294689016 27.0238121792485 24.042013620195913 96-97 23.280944584539537 25.59324992826054 26.99908593850867 24.126719548691252 98-99 22.99276576336453 25.954306179518085 26.46582977361087 24.58709828350651 100-101 23.544887446438185 25.188518929555308 26.98912705582657 24.277466568179932 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 470.0 1 320.0 2 269.0 3 369.5 4 309.5 5 303.5 6 538.5 7 1181.0 8 1858.0 9 2064.0 10 1808.5 11 1591.5 12 1620.0 13 1694.5 14 1850.0 15 2010.5 16 2145.5 17 2307.5 18 2519.5 19 2778.0 20 3156.5 21 3692.5 22 4325.5 23 5028.0 24 5942.5 25 7429.5 26 9330.5 27 11605.5 28 14048.5 29 16641.5 30 19893.0 31 24914.0 32 32445.5 33 35212.5 34 34772.5 35 37924.5 36 42501.5 37 47268.0 38 51569.5 39 54762.0 40 58795.5 41 65173.5 42 70037.5 43 73567.0 44 77216.0 45 82410.5 46 85958.5 47 89024.5 48 93626.5 49 92965.5 50 90216.0 51 88204.5 52 87099.5 53 88636.0 54 89863.0 55 85656.0 56 78845.0 57 73315.0 58 68686.5 59 61264.5 60 50296.0 61 41900.0 62 35433.0 63 27248.5 64 20176.5 65 15838.0 66 12243.5 67 9662.0 68 7435.5 69 5449.0 70 4118.0 71 3049.0 72 2274.0 73 1789.5 74 1408.0 75 1087.0 76 801.0 77 503.0 78 291.5 79 198.0 80 123.5 81 72.5 82 46.5 83 32.0 84 20.5 85 11.0 86 8.5 87 8.5 88 6.5 89 3.5 90 2.5 91 2.5 92 2.0 93 3.0 94 2.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006474168069403081 2 0.10804571877414415 3 0.11546314311855961 4 0.11855016365496371 5 0.16061081846346983 6 0.09604063891035036 7 0.028426314106054586 8 0.027440182545814387 9 0.024910540717372122 10-11 0.051257403489876716 12-13 0.055287680301293204 14-15 0.028319125892985002 16-17 0.03262809205838242 18-19 0.03762306278742519 20-21 0.05968239703714629 22-23 0.03305684491066077 24-25 0.05076433770975662 26-27 0.05818176205417207 28-29 0.05415148524275557 30-31 0.06890058336113082 32-33 0.051300278775104555 34-35 0.0632624833536705 36-37 0.05348691832172414 38-39 0.0326066544157685 40-41 0.03661549358457107 42-43 0.03254234148792675 44-45 0.007588925485326792 46-47 0.01365577834506544 48-49 0.009496875677965448 50-51 0.008875184042161841 52-53 0.009775565031946376 54-55 0.010483007238205652 56-57 0.01399878062688812 58-59 0.018886563142861308 60-61 0.024739039576460783 62-63 0.010483007238205652 64-65 0.008853746399547923 66-67 0.009689814461490705 68-69 0.011576327011515444 70-71 0.030591516010060257 72-73 0.027954685968548408 74-75 0.028876504600946856 76-77 0.023152654023030888 78-79 0.00469484373244793 80-81 0.004394716735853085 82-83 0.005895351718827309 84-85 0.00469484373244793 86-87 0.012348082145616474 88-89 0.038116128567545295 90-91 0.03571511259478653 92-93 0.029734010305503558 94-95 0.047248564321074146 96-97 0.04257515823124013 98-99 0.025146354786125213 100-101 0.07479593507995812 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2332346.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.9297005557156 #Duplication Level Percentage of deduplicated Percentage of total 1 76.1228374876791 38.007904812112194 2 12.72486137288647 12.70697035922427 3 4.373565462948586 6.551124417775278 4 2.0640376825777937 4.122271337072896 5 1.1746476926599918 2.932490377648782 6 0.7218721745507415 2.162571690889307 7 0.5021178622363234 1.7549416153594979 8 0.3569883536848739 1.4259457281090915 9 0.2667426868330431 1.1986544231100777 >10 1.5018700940842005 13.867749853312386 >50 0.11837317115812622 4.05601638142614 >100 0.06448437879292555 6.055002733246559 >500 0.0038007899539069774 1.3672231921540343 >1k 0.00362802677418384 3.27909289757366 >5k 1.7276317972304E-4 0.5120401809858801 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6710 0.28769316387877275 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5162 0.22132222234608417 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3712 0.15915305876572344 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3695 0.15842417891685023 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3457 0.14821986103262552 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3452 0.14800548460648635 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2987 0.12806847697554308 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2881 0.12352369674139257 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2718 0.11653502524925548 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2536 0.10873172333778951 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2500 0.10718821306958744 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2414 0.10350093853999363 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2393 0.10260055755020911 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.575057045566996E-5 2 0.0 0.0 0.0 0.0 8.575057045566996E-5 3 0.0 0.0 0.0 0.0 8.575057045566996E-5 4 0.0 0.0 0.0 0.0 8.575057045566996E-5 5 0.0 0.0 0.0 0.0 1.2862585568350492E-4 6 0.0 0.0 0.0 0.0 1.7150114091133993E-4 7 0.0 0.0 0.0 0.0 1.7150114091133993E-4 8 0.0 0.0 0.0 0.0 2.143764261391749E-4 9 0.0 0.0 0.0 4.287528522783498E-5 2.143764261391749E-4 10-11 0.0 0.0 0.0 4.287528522783498E-5 2.5725171136700985E-4 12-13 0.0 0.0 0.0 4.287528522783498E-5 6.002539931896897E-4 14-15 0.0 0.0 0.0 8.575057045566996E-5 6.431292784175247E-4 16-17 0.0 0.0 0.0 1.2862585568350492E-4 6.860045636453597E-4 18-19 0.0 0.0 0.0 1.715011409113399E-4 7.074422062592772E-4 20-21 0.0 0.0 0.0 2.7868935398092735E-4 9.64693917626287E-4 22-23 0.0 0.0 0.0 4.7162813750618475E-4 0.0012005079863793794 24-25 0.0 0.0 0.0 7.074422062592772E-4 0.0012433832716072144 26-27 0.0 0.0 0.0 0.0014577596977463892 0.0013291338420628844 28-29 0.0 0.0 0.0 0.0037515874574355606 0.0013291338420628844 30-31 0.0 0.0 0.0 0.011297637657534516 0.0013291338420628844 32-33 0.0 0.0 0.0 0.028147624752073663 0.0013291338420628844 34-35 0.0 0.0 0.0 0.056102310720622064 0.0013291338420628844 36-37 0.0 0.0 0.0 0.10437988188716425 0.0013291338420628844 38-39 0.0 0.0 0.0 0.18014051088474864 0.0013291338420628844 40-41 0.0 0.0 0.0 0.2576590265766743 0.0013291338420628844 42-43 0.0 0.0 0.0 0.34210190083289527 0.0013291338420628844 44-45 0.0 0.0 0.0 0.4468247850018823 0.0013720091272907192 46-47 0.0 0.0 0.0 0.5704556699563443 0.0014148844125185542 48-49 0.0 0.0 0.0 0.7045909997916261 0.0014148844125185542 50-51 0.0 0.0 0.0 0.8540971193810867 0.0014148844125185542 52-53 0.0 0.0 0.0 0.9988226446676437 0.0014577596977463892 54-55 0.0 0.0 0.0 1.1552102475361716 0.0015220726255881418 56-57 0.0 0.0 0.0 1.3376660238232234 0.0015863855534298942 58-59 0.0 0.0 0.0 1.5338847666684103 0.0016292608386577292 60-61 0.0 0.0 0.0 1.7551855513718806 0.0016721361238855642 62-63 0.0 0.0 0.0 1.973013437971896 0.0016721361238855642 64-65 0.0 0.0 0.0 2.202246150442516 0.0017364490517273166 66-67 0.0 0.0 0.0 2.464578583109024 0.0017578866943412342 68-69 0.0 0.0 0.0 2.754608450032714 0.0017578866943412342 70-71 0.0 0.0 0.0 3.0664618371373713 0.0018007619795690692 72-73 0.0 0.0 0.0 3.3880693516313616 0.002015138405708244 74-75 0.0 0.0 0.0 3.7126995737339143 0.002250952474461336 76-77 0.0 0.0 0.0 4.057202490539568 0.002272390117075254 78-79 0.0 0.0 0.0 4.448439468243563 0.0022938277596891714 80-81 0.0 0.0 0.0 4.862764787042746 0.002315265402303089 82-83 0.0 0.0 0.0 5.282428078852795 0.002422453615372676 84-85 0.0 0.0 0.0 5.705864395762893 0.002465328900600511 86-87 0.0 0.0 0.0 6.148980468592567 0.002615392398897934 88-89 0.0 0.0 0.0 6.6168355809987025 0.002744018254581439 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9140 0.0 36.26204 1 GGTATCA 5760 0.0 35.085857 1 ATCAACG 11445 0.0 28.710882 3 TATCAAC 11615 0.0 28.372545 2 TCAACGC 11615 0.0 28.290663 4 CAACGCA 11790 0.0 27.951408 5 AGGTAAG 1180 0.0 27.403824 2 GTACATG 10020 0.0 27.287624 1 AACGCAG 12125 0.0 27.237698 6 TACATGG 10000 0.0 27.058052 2 GTAAGGT 1350 0.0 25.71422 4 GTGTAAG 840 0.0 25.4741 1 ACATGGG 10335 0.0 25.444794 3 TAAGGTG 1470 0.0 25.232574 5 AAGGTAA 1250 0.0 24.346447 1 ACGCAGA 13625 0.0 23.95747 7 CGCAGAG 13685 0.0 23.713043 8 GGTAAGG 1485 0.0 23.056337 3 TAAGGTA 625 0.0 22.825773 4 GAGTACT 6585 0.0 22.727283 12-13 >>END_MODULE