FastQCFastQC Report
Thu 26 May 2016
SRR938555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332346
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68800.2949819623675046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55570.23825796001107896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.1690143743681255No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36940.1583813036316224No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36370.15593741237363581No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32460.13917317584955233No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30980.13282763363583278No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29080.1246813294425441No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28690.12300919331865855No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA24980.10710246249913177No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24680.10581620394229674No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24070.1032008115433988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65050.035.4396551
GTATCAA98400.033.427671
TAAGGTG14400.026.723545
GTAAGGT13400.026.5905884
ATCAACG122600.026.5830693
TATCAAC123200.026.38952
TCAACGC125450.026.0170174
CAACGCA126500.025.8010675
GTACATG102450.025.6571391
TACATGG101500.025.5689182
AACGCAG129450.025.2373456
ACATGGG103650.024.2011553
AGGTAAG13550.023.853682
AAGGTAA14500.021.9635161
ACGCAGA148600.021.9530687
CGCAGAG150450.021.6199978
GAGTACT73700.021.58413112-13
GGACCGA8900.021.3416146
CATGGGG55350.021.1148954
GTACTTT75500.020.94375414-15
Produced by FastQC (version 0.11.5)