##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938555_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2332346 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79581502915948 31.0 31.0 34.0 30.0 34.0 2 32.017656471209676 31.0 31.0 34.0 30.0 34.0 3 32.0781727925445 33.0 31.0 34.0 30.0 34.0 4 35.75643493718342 37.0 35.0 37.0 35.0 37.0 5 35.64831332915442 37.0 35.0 37.0 33.0 37.0 6 35.654241266090025 37.0 35.0 37.0 33.0 37.0 7 35.592569884571155 37.0 35.0 37.0 33.0 37.0 8 35.56858930879038 37.0 35.0 37.0 33.0 37.0 9 37.15007764714155 39.0 37.0 39.0 33.0 39.0 10-11 37.04741663543916 39.0 37.0 39.0 33.0 39.0 12-13 36.938698846569075 39.0 37.0 39.0 33.0 39.0 14-15 37.98210771472157 40.0 37.0 41.0 33.0 41.0 16-17 37.89883512137564 40.0 37.0 41.0 33.0 41.0 18-19 38.13170580179785 40.0 37.0 41.0 33.0 41.0 20-21 38.09444910832269 40.0 37.0 41.0 33.0 41.0 22-23 37.98843846496189 40.0 37.0 41.0 33.0 41.0 24-25 37.85821872055004 40.0 37.0 41.0 32.0 41.0 26-27 37.662163546917995 40.0 37.0 41.0 32.0 41.0 28-29 37.557813463354066 39.5 36.5 41.0 32.0 41.0 30-31 37.30427303667638 39.0 36.0 41.0 31.0 41.0 32-33 37.10897225368792 39.0 36.0 41.0 31.0 41.0 34-35 37.01126033615938 39.0 36.0 41.0 31.0 41.0 36-37 36.9693819013131 39.0 36.0 41.0 31.0 41.0 38-39 36.81047794795455 39.0 35.0 41.0 30.0 41.0 40-41 36.641260773487296 39.0 35.0 40.5 30.0 41.0 42-43 36.41762007009252 39.0 35.0 40.0 30.0 41.0 44-45 36.12182497794066 38.5 35.0 40.0 29.5 41.0 46-47 35.739295113160736 38.0 35.0 40.0 28.5 41.0 48-49 35.36793190204198 38.0 34.0 40.0 27.0 41.0 50-51 34.982778498558964 38.0 34.0 40.0 26.0 41.0 52-53 34.482342028155344 38.0 33.0 40.0 24.5 41.0 54-55 34.135394576962426 37.0 33.0 40.0 24.0 41.0 56-57 33.79092210160928 37.0 33.0 40.0 23.0 41.0 58-59 33.29991219141586 36.0 32.0 39.0 22.5 40.5 60-61 32.888925571077365 36.0 31.0 39.0 22.0 40.0 62-63 32.401253287462495 35.0 31.0 39.0 20.0 40.0 64-65 31.864023819793463 35.0 30.5 38.0 18.5 40.0 66-67 31.351675737647845 35.0 30.0 38.0 17.5 40.0 68-69 30.849225843849926 34.0 29.0 37.0 13.5 39.0 70-71 30.266969609140325 34.0 29.0 36.5 9.0 39.0 72-73 29.771888261861662 34.0 28.5 36.0 7.5 39.0 74-75 29.184019652315737 33.5 27.5 35.5 7.0 38.0 76-77 27.97679225123545 31.5 26.0 34.5 3.5 36.0 78-79 28.678142951345983 33.0 27.5 35.0 2.0 37.0 80-81 28.30817490200854 33.0 27.0 35.0 2.0 36.5 82-83 27.870540648771666 33.0 26.0 35.0 2.0 36.0 84-85 27.330007640375825 32.5 26.0 35.0 2.0 35.5 86-87 26.72921170358086 32.0 25.0 34.5 2.0 35.0 88-89 25.9746750696509 31.5 23.5 34.0 2.0 35.0 90-91 25.36934485706666 31.0 21.5 34.0 2.0 35.0 92-93 24.548609640250632 31.0 18.5 34.0 2.0 35.0 94-95 23.732038256759502 31.0 10.5 34.0 2.0 35.0 96-97 22.652932498008443 30.0 2.0 34.0 2.0 35.0 98-99 21.27634643401965 29.5 2.0 34.0 2.0 35.0 100-101 17.351333592871725 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24819951811030094 1101 2 0.330537617222987 1101 3 0.3095841035771798 1101 4 0.2372024226199798 1101 5 0.14839928163031146 1101 6 0.28339131061844114 1101 7 0.2833079981491977 1101 8 0.2721255569127834 1101 9 0.34969065048229453 1101 10-11 0.30895599869320733 1101 12-13 0.3819814312424299 1101 14-15 0.3836257124536502 1101 16-17 0.41940995991031826 1101 18-19 0.48861033008461874 1101 20-21 0.4435665430760807 1101 22-23 0.47965256171989523 1101 24-25 0.5083684920729326 1101 26-27 0.4580286739786388 1101 28-29 0.522480795926306 1101 30-31 0.6111632276982419 1101 32-33 0.4977181728734408 1101 34-35 0.5062313966964567 1101 36-37 0.6304874496171209 1101 38-39 0.7146902662892103 1101 40-41 0.7811349450715426 1101 42-43 0.7973191049194241 1101 44-45 0.8225828322655033 1101 46-47 0.6253477916968677 1101 48-49 0.5978682832713602 1101 50-51 0.838778994438151 1101 52-53 0.8195296353027786 1101 54-55 0.7729333398357383 1101 56-57 0.7454462671455886 1101 58-59 0.9255898538540634 1101 60-61 0.9826315369502652 1101 62-63 0.9864574157632049 1101 64-65 0.9842887557792643 1101 66-67 0.872135474004498 1101 68-69 0.8983371439517747 1101 70-71 0.8337008522143279 1101 72-73 0.7975424937917417 1101 74-75 0.8709984760958598 1101 76-77 1.012394768826944 1101 78-79 1.0411121764248712 1101 80-81 1.238209850705747 1101 82-83 1.0611445906730061 1101 84-85 1.0388863150897727 1101 86-87 1.2079812494818363 1101 88-89 1.2441031940543823 1101 90-91 1.1759278465238694 1101 92-93 1.4411709870560543 1101 94-95 1.3861230497155077 1101 96-97 1.4927925109814808 1101 98-99 1.4505134833689368 1101 100-101 1.7240204930782603 1102 1 0.20172185955249589 1102 2 0.228102240747738 1102 3 0.19937486864823484 1102 4 0.10377300541656354 1102 5 -0.048039529001584924 1102 6 0.0371871414590359 1102 7 0.09073105983193841 1102 8 0.179860178961313 1102 9 0.09195253212043042 1102 10-11 0.28501441043460574 1102 12-13 0.23126060797718395 1102 14-15 0.32710159177645437 1102 16-17 0.2695408774206314 1102 18-19 0.2648534637137132 1102 20-21 0.2945814451404587 1102 22-23 0.25743776376796035 1102 24-25 0.26114009734692445 1102 26-27 0.2558028820636693 1102 28-29 0.19415521922883983 1102 30-31 0.332753351001557 1102 32-33 0.4096538096297948 1102 34-35 0.41445550261144604 1102 36-37 0.374169805666142 1102 38-39 0.35807116243316983 1102 40-41 0.37707572529328814 1102 42-43 0.15298235555847128 1102 44-45 0.24423666616730344 1102 46-47 0.3839065724935651 1102 48-49 0.36465350085858717 1102 50-51 0.5841795942501236 1102 52-53 0.7009261405433662 1102 54-55 0.6099274427205899 1102 56-57 0.6226435058350219 1102 58-59 0.6164514620028072 1102 60-61 0.647011290965203 1102 62-63 0.7276669070884552 1102 64-65 0.877313999161526 1102 66-67 0.8807977845237396 1102 68-69 0.8175758022998032 1102 70-71 0.8884094150741575 1102 72-73 0.8613575932035822 1102 74-75 0.8251031479474307 1102 76-77 0.728571239415178 1102 78-79 0.8198093335336551 1102 80-81 0.8076310943055525 1102 82-83 0.9160635150516292 1102 84-85 0.9788241195603895 1102 86-87 0.8666711420284692 1102 88-89 1.051887735687707 1102 90-91 1.0282369672399128 1102 92-93 1.3194375149061344 1102 94-95 1.3680535196977175 1102 96-97 1.1731890706091654 1102 98-99 1.3687098518648249 1102 100-101 1.554109571372372 1103 1 0.2987668166034858 1103 2 0.25322058780418644 1103 3 0.25623113777952966 1103 4 0.19551607366040002 1103 5 0.16363337634449948 1103 6 0.19212501666962822 1103 7 0.27269077140918085 1103 8 0.2589992115408606 1103 9 0.23492519365377973 1103 10-11 0.2450904461938137 1103 12-13 0.373513436145565 1103 14-15 0.5485025361800311 1103 16-17 0.5085524347967052 1103 18-19 0.48911334969715625 1103 20-21 0.4280140436740254 1103 22-23 0.37398032421690885 1103 24-25 0.43737284210880745 1103 26-27 0.45527439868565267 1103 28-29 0.5393498912384231 1103 30-31 0.4858227738594536 1103 32-33 0.46935511678638164 1103 34-35 0.4876955350604817 1103 36-37 0.5976117918797428 1103 38-39 0.5604377698768701 1103 40-41 0.5935583336149577 1103 42-43 0.6631152661248834 1103 44-45 0.8226523431480715 1103 46-47 0.6994531193696787 1103 48-49 0.8442451219463152 1103 50-51 0.960035497069093 1103 52-53 0.8959348015309558 1103 54-55 0.7670361262504741 1103 56-57 0.8858735722161484 1103 58-59 1.1186626833207072 1103 60-61 0.9882237995668675 1103 62-63 0.9266395641726746 1103 64-65 0.8590222973961055 1103 66-67 0.9434599926603191 1103 68-69 1.0086217648558637 1103 70-71 0.7793328780454694 1103 72-73 0.9232708632009725 1103 74-75 0.8949438539958834 1103 76-77 0.697867741799918 1103 78-79 0.9635077606152578 1103 80-81 0.9102324679025919 1103 82-83 1.1656766254732922 1103 84-85 1.3957570547453564 1103 86-87 1.3162892948059195 1103 88-89 1.203688968988608 1103 90-91 1.282076943629825 1103 92-93 1.5018326858123352 1103 94-95 1.2474092252715963 1103 96-97 1.2888905586973394 1103 98-99 1.668380022474416 1103 100-101 1.848453568299501 1104 1 0.19125565314474358 1104 2 0.22933022722027374 1104 3 0.21531545472674907 1104 4 0.13398466787496233 1104 5 0.0953930671767651 1104 6 0.09690134231288994 1104 7 0.14735859214555092 1104 8 0.18558654639432604 1104 9 0.19538188949354662 1104 10-11 0.2924558559584085 1104 12-13 0.25345368579346683 1104 14-15 0.33902072185951226 1104 16-17 0.34628985040710347 1104 18-19 0.2648839636734124 1104 20-21 0.20006152539892952 1104 22-23 0.2811772621598152 1104 24-25 0.19625488249286605 1104 26-27 0.3343517157669922 1104 28-29 0.4075038896847687 1104 30-31 0.3046106024759041 1104 32-33 0.3310405266068699 1104 34-35 0.5380909017360977 1104 36-37 0.5146462658698567 1104 38-39 0.358271409192362 1104 40-41 0.49934534348462023 1104 42-43 0.5645449992078326 1104 44-45 0.5339221970590486 1104 46-47 0.6685311070968538 1104 48-49 0.7864401667119196 1104 50-51 0.8673279671135887 1104 52-53 0.7478394356936562 1104 54-55 0.6675493100297842 1104 56-57 0.744603948839675 1104 58-59 0.8621001344194426 1104 60-61 0.9208315779407741 1104 62-63 1.1110802665065194 1104 64-65 0.8993750920331713 1104 66-67 0.866730948945392 1104 68-69 0.9482189302594826 1104 70-71 1.008200548445398 1104 72-73 0.932975492613263 1104 74-75 1.114454268566785 1104 76-77 0.9151863321023761 1104 78-79 1.1751714443051497 1104 80-81 1.1091172048417661 1104 82-83 1.0972129423422707 1104 84-85 1.0836012846762806 1104 86-87 1.3768145571961021 1104 88-89 1.3971461901676037 1104 90-91 1.2717717019737762 1104 92-93 1.6521626645193308 1104 94-95 1.5391499424155377 1104 96-97 1.3788795279705859 1104 98-99 1.7531720104922073 1104 100-101 1.7723382353797312 1105 1 0.14601387984319913 1105 2 0.2274742532343197 1105 3 0.1442643916216042 1105 4 0.14753702843871253 1105 5 0.08291110996046314 1105 6 0.1922878633398284 1105 7 0.1644860593441777 1105 8 0.1391385420478386 1105 9 0.2531502201508715 1105 10-11 0.19751989770414013 1105 12-13 0.24880462807278292 1105 14-15 0.3159685315872025 1105 16-17 0.2698426772450375 1105 18-19 0.33453873832106495 1105 20-21 0.22654235139351186 1105 22-23 0.2057873150312517 1105 24-25 0.25618936083341737 1105 26-27 0.0839812305174803 1105 28-29 0.20103869401179253 1105 30-31 0.2994364794768316 1105 32-33 0.28691578407131857 1105 34-35 0.3808612191268992 1105 36-37 0.3378989540419042 1105 38-39 0.26740622730488184 1105 40-41 0.4597383923501539 1105 42-43 0.5211019292585988 1105 44-45 0.5163046351459499 1105 46-47 0.3529126450718536 1105 48-49 0.371027878968043 1105 50-51 0.37169117848581124 1105 52-53 0.4292880076782737 1105 54-55 0.27904813826248187 1105 56-57 0.26310236350744276 1105 58-59 0.3367226550571445 1105 60-61 0.23882550421268434 1105 62-63 0.32780957609636374 1105 64-65 0.1794948839532502 1105 66-67 0.28539245821411185 1105 68-69 0.396086435408769 1105 70-71 0.3008783205467971 1105 72-73 0.37022563046824075 1105 74-75 0.4603612934942056 1105 76-77 0.3186447688269425 1105 78-79 0.5078641574532696 1105 80-81 0.5393767776535192 1105 82-83 0.6539640857276616 1105 84-85 0.8386213344662572 1105 86-87 0.8633726833415132 1105 88-89 0.8924337799760664 1105 90-91 0.9123183983198082 1105 92-93 1.0870028743832592 1105 94-95 1.1865376919735908 1105 96-97 1.3438126683069527 1105 98-99 1.3555708898123413 1105 100-101 1.299689270913877 1106 1 0.06291720579942961 1106 2 0.08617096321082585 1106 3 0.07789855957449987 1106 4 0.07534250437058176 1106 5 -0.02714685880527412 1106 6 -0.008160513289737992 1106 7 0.0701022173720176 1106 8 0.13346909739923518 1106 9 -0.2294423066557698 1106 10-11 0.153303486649925 1106 12-13 0.06013111444964636 1106 14-15 0.12687078364449178 1106 16-17 0.1267275230200795 1106 18-19 0.04572176595389266 1106 20-21 0.18827440816179575 1106 22-23 0.22426247720496661 1106 24-25 0.2518604183720754 1106 26-27 0.10317606831655013 1106 28-29 0.1613114085397811 1106 30-31 0.1746970428858674 1106 32-33 0.06309377227339752 1106 34-35 0.18077131922136402 1106 36-37 0.21573631347547462 1106 38-39 0.07268131352984852 1106 40-41 0.21269093391099858 1106 42-43 0.21224632919544462 1106 44-45 0.14580158698790768 1106 46-47 -0.4279387260888612 1106 48-49 -0.03675436658324571 1106 50-51 0.30053525012807114 1106 52-53 0.17418035237125906 1106 54-55 0.455191469744733 1106 56-57 0.20261685978214672 1106 58-59 0.02636653982193593 1106 60-61 0.10268448317842171 1106 62-63 -0.05645280232439376 1106 64-65 0.057601313599935366 1106 66-67 -0.007502250689856993 1106 68-69 -0.12462508049010879 1106 70-71 0.06307937443773781 1106 72-73 0.39128311808913807 1106 74-75 0.22280819402312702 1106 76-77 0.2112806497099058 1106 78-79 0.4772144826173488 1106 80-81 -0.047007776168008775 1106 82-83 0.41570168893221293 1106 84-85 0.39168243986079077 1106 86-87 0.6720789889930252 1106 88-89 0.8691385775413494 1106 90-91 0.788021996085444 1106 92-93 0.8380878321759013 1106 94-95 1.059201468368503 1106 96-97 0.9130228353669736 1106 98-99 0.9788081098704353 1106 100-101 0.9057376426435262 1107 1 0.15771094218067816 1107 2 0.21484013746137265 1107 3 0.23375738714717187 1107 4 0.11799349059346298 1107 5 0.04404974701624553 1107 6 0.007004580373113356 1107 7 0.13962339623831355 1107 8 0.1894158512989037 1107 9 0.28267472409820726 1107 10-11 0.2292340319838999 1107 12-13 0.11297946467409048 1107 14-15 0.3450786822185421 1107 16-17 0.38086081508559744 1107 18-19 0.24789716711672583 1107 20-21 0.29606219935659084 1107 22-23 0.3669919850713157 1107 24-25 0.33105407374367246 1107 26-27 0.3040006450933461 1107 28-29 0.37517689768035467 1107 30-31 0.37415843815277583 1107 32-33 0.35935440707128663 1107 34-35 0.41149408159088807 1107 36-37 0.328266337554453 1107 38-39 0.19267694789898115 1107 40-41 0.2740290428995067 1107 42-43 0.29095648099566773 1107 44-45 0.16421046713226417 1107 46-47 0.20392920163473605 1107 48-49 0.35225825941173383 1107 50-51 0.33804144320340157 1107 52-53 0.29974183437024493 1107 54-55 0.19878877944130124 1107 56-57 0.2988575407276741 1107 58-59 0.19129781845583693 1107 60-61 0.33060549577920995 1107 62-63 0.6441807781016493 1107 64-65 0.33184147073610504 1107 66-67 0.2653922239507551 1107 68-69 0.545720691739735 1107 70-71 0.43160679174411243 1107 72-73 0.4499622891699495 1107 74-75 0.3402683787894958 1107 76-77 0.5726396911686393 1107 78-79 0.8142747742686964 1107 80-81 0.7400028464800954 1107 82-83 0.8562424433072557 1107 84-85 1.010361999071403 1107 86-87 1.1241034093236628 1107 88-89 1.1662773974708571 1107 90-91 1.316467348819959 1107 92-93 1.5133197272892964 1107 94-95 1.722581305954428 1107 96-97 1.7601557587091818 1107 98-99 1.486698340792735 1107 100-101 1.7982090193150349 1108 1 0.2058004774600768 1108 2 0.0945703283854229 1108 3 0.09320742554784545 1108 4 0.11647795068799383 1108 5 0.018563392621750552 1108 6 0.06689064255272115 1108 7 0.10547852701296989 1108 8 0.125124080135933 1108 9 0.06887616548508646 1108 10-11 0.1399518422510937 1108 12-13 0.2592223198253478 1108 14-15 0.2940347685378555 1108 16-17 0.3617136947863102 1108 18-19 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2316 72-73 -1.103636773169299 2316 74-75 -1.0338573831053637 2316 76-77 -1.0118366395652458 2316 78-79 -1.2863193378531754 2316 80-81 -1.4050917658918571 2316 82-83 -1.415883509521489 2316 84-85 -1.458292911428753 2316 86-87 -1.7686990450867377 2316 88-89 -1.9283434126348098 2316 90-91 -1.7090639362924769 2316 92-93 -1.7607147371039318 2316 94-95 -1.8733948203610744 2316 96-97 -2.1392719690932225 2316 98-99 -2.218374825435397 2316 100-101 -2.486912681181181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 46.0 5 77.0 6 188.0 7 436.0 8 1081.0 9 1799.0 10 3069.0 11 4690.0 12 6406.0 13 9127.0 14 11991.0 15 15673.0 16 19874.0 17 24961.0 18 23997.0 19 21456.0 20 21164.0 21 22463.0 22 24651.0 23 27717.0 24 31953.0 25 37703.0 26 44633.0 27 53572.0 28 63886.0 29 75677.0 30 89975.0 31 106479.0 32 128599.0 33 158147.0 34 200179.0 35 260285.0 36 333976.0 37 352519.0 38 149153.0 39 4731.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18875426932849 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23.118439545419076 26.299099704760785 28.305277175856414 22.277183573963725 20-21 23.41440763934682 25.45156250401956 28.50604069893575 22.627989157697872 22-23 23.31530141754268 25.342702154826085 28.43409597032344 22.907900457307793 24-25 23.260784834149234 25.666037929205505 28.364449334465892 22.70872790217937 26-27 22.566555517491096 26.099054110082704 28.299756335052805 23.03463403737339 28-29 22.703116391442947 26.540970568247836 28.167702462543065 22.588210577766155 30-31 22.67781902936199 25.878156378614936 28.071912673364203 23.37211191865887 32-33 22.457606316761165 25.94698573467914 28.015985700164364 23.579422248395332 34-35 23.414391087027507 26.045711058649186 27.80831466065539 22.73158319366792 36-37 23.492957492787664 25.484085258874273 27.841372794646468 23.1815844536916 38-39 22.795180433599757 25.91708925699423 27.978988467325234 23.308741842080778 40-41 23.2768330575706 25.74817166948432 27.99466968283498 22.980325590110102 42-43 22.98219534950727 25.77764038430536 27.67230542054408 23.567858845643293 44-45 22.968957964571413 25.97563255533144 27.997905775326227 23.05750370477092 46-47 23.483822726130686 26.383113826164728 27.090899034705828 23.04216441299876 48-49 23.200524278987768 26.372202066074244 27.113172745381686 23.314100909556302 50-51 23.328329037556657 26.482814713787224 27.1944208569158 22.994435391740318 52-53 23.762940832963892 26.19754959169866 26.995908840283562 23.04360073505389 54-55 22.964614167924545 26.438390200932254 27.273980423216788 23.32301520792641 56-57 23.12244838458788 26.62493043484972 27.060800584475885 23.191820596086515 58-59 23.13966491228258 26.04341537732481 27.001933074660855 23.814986635731756 60-61 22.677853114417843 26.41269777297193 27.522353029953532 23.38709608265669 62-63 22.812136056381995 26.40391116645484 27.57050322199982 23.213449555163347 64-65 22.42415912887443 26.448800939462597 27.29656001370514 23.83047991795783 66-67 22.48851609461889 26.44754236063558 27.256721938343443 23.807219606402086 68-69 23.024986985051893 25.937633740920624 27.493080850487196 23.54429842354029 70-71 22.81183161406902 26.25727031319773 27.101142219770708 23.829755852962546 72-73 22.840391468839933 26.295263477428716 27.039407565950818 23.824937487780534 74-75 22.682874948752303 26.775957924566818 26.830100368297273 23.71106675838361 76-77 22.783903038331328 26.302684342650057 27.14325731356396 23.77015530545465 78-79 22.958433781703693 26.489634500171338 27.095326915216788 23.45660480290818 80-81 22.994858928791608 26.656288028648472 26.87709140813887 23.471761634421053 82-83 22.909569104432222 26.671557667125747 26.84069821354814 23.57817501489389 84-85 22.70609292867612 26.672311364324425 26.918260947125667 23.70333475987379 86-87 23.001899165422067 26.740476407940218 26.566254030205695 23.69137039643202 88-89 22.70114615756464 26.870817556922205 26.36454200149634 24.06349428401681 90-91 22.59373035267195 26.75077259834064 26.700016554147073 23.95548049484034 92-93 22.764889585738523 26.302104329597675 26.86056564901079 24.072440435653007 94-95 22.460156105422946 26.15483124175626 27.023727603992548 24.361285048828243 96-97 22.691678000946204 25.87484124502503 27.015625221163216 24.417855532865552 98-99 22.437890297060115 26.204218050283195 26.597534101190135 24.760357551466555 100-101 22.39220645451228 25.910374055363228 27.083861317909534 24.61355817221495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 470.0 1 323.5 2 251.0 3 356.0 4 332.0 5 318.5 6 583.0 7 1227.0 8 1871.0 9 2103.5 10 1871.5 11 1583.0 12 1557.5 13 1718.5 14 1933.0 15 2049.0 16 2170.5 17 2389.0 18 2580.5 19 2797.0 20 3138.5 21 3570.0 22 4162.0 23 5000.0 24 6079.5 25 7483.0 26 9326.0 27 11719.5 28 14203.5 29 17029.0 30 20401.0 31 25484.5 32 33543.0 33 36354.0 34 35177.0 35 37770.0 36 42497.0 37 47398.5 38 51890.5 39 55226.0 40 58312.0 41 64935.0 42 70499.0 43 74193.0 44 77997.5 45 83157.0 46 86780.5 47 90445.5 48 95575.0 49 94326.5 50 91252.5 51 89011.0 52 87448.0 53 89582.5 54 91753.0 55 86819.0 56 79168.0 57 72767.5 58 67498.5 59 60086.5 60 48499.0 61 40067.5 62 34130.5 63 25762.5 64 18460.5 65 14193.5 66 10745.0 67 8610.0 68 6648.5 69 4709.0 70 3480.0 71 2487.5 72 1863.0 73 1573.5 74 1241.5 75 956.0 76 672.5 77 393.0 78 218.5 79 127.0 80 87.5 81 45.0 82 26.0 83 19.0 84 9.5 85 3.5 86 1.5 87 1.5 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.145034227340198E-4 2 0.04909220158587105 3 0.0 4 4.287528522783498E-5 5 0.046305308046061776 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006817170351225761 26-27 0.001929387835252574 28-29 4.287528522783498E-5 30-31 0.001929387835252574 32-33 4.930657801201023E-4 34-35 0.01219801864731905 36-37 0.024739039576460783 38-39 0.004416154378467003 40-41 0.0011576327011515444 42-43 0.0014577596977463894 44-45 0.009346812179668025 46-47 0.0 48-49 0.0 50-51 7.074422062592772E-4 52-53 0.0 54-55 0.0018221996221829866 56-57 0.0 58-59 0.024374599652024185 60-61 0.0 62-63 0.010890322447870084 64-65 0.0160567943178242 66-67 0.007245923203504112 68-69 0.01766461751386801 70-71 6.431292784175248E-5 72-73 8.575057045566996E-5 74-75 0.022895402311663877 76-77 0.051364591702946306 78-79 0.03074157950835768 80-81 0.02716149319183346 82-83 0.03657261829934323 84-85 0.015606603822931933 86-87 0.011533451726287608 88-89 0.08551475638691686 90-91 0.025896672277612325 92-93 0.02495341600259996 94-95 0.02797612361116232 96-97 0.039573888265291686 98-99 0.05440873695412259 100-101 0.015649479108159767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2332346.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.158312155125905 #Duplication Level Percentage of deduplicated Percentage of total 1 73.69351203283031 34.75261644251739 2 13.811755531588268 13.026781575378532 3 4.964520223883458 7.02355183254995 4 2.328385729341425 4.3921096416729375 5 1.2925164193885725 3.047644638557597 6 0.7920048551651333 2.240976731295158 7 0.5539778333977254 1.8287261716073189 8 0.385778051298387 1.4554113412580405 9 0.308283098434442 1.308429952930874 >10 1.6529961476955581 14.54085767081333 >50 0.13492137859220887 4.3693592226915525 >100 0.07252690670502555 6.4979995576150085 >500 0.004547315298666637 1.4782463003093402 >1k 0.004092583768799873 3.5038816257977166 >5k 1.8189261194666103E-4 0.5334072950052782 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6880 0.2949819623675046 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5557 0.23825796001107896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3942 0.1690143743681255 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3694 0.1583813036316224 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3637 0.15593741237363581 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3246 0.13917317584955233 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3098 0.13282763363583278 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2908 0.1246813294425441 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2869 0.12300919331865855 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2498 0.10710246249913177 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2468 0.10581620394229674 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2407 0.1032008115433988 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.575057045566996E-5 2 0.0 0.0 0.0 0.0 8.575057045566996E-5 3 0.0 0.0 0.0 0.0 8.575057045566996E-5 4 0.0 0.0 0.0 0.0 8.575057045566996E-5 5 0.0 0.0 0.0 0.0 1.2862585568350492E-4 6 0.0 0.0 0.0 0.0 2.5725171136700985E-4 7 0.0 0.0 0.0 0.0 2.5725171136700985E-4 8 0.0 0.0 0.0 0.0 2.5725171136700985E-4 9 0.0 0.0 0.0 4.287528522783498E-5 2.5725171136700985E-4 10-11 0.0 0.0 0.0 4.287528522783498E-5 2.5725171136700985E-4 12-13 0.0 0.0 0.0 4.287528522783498E-5 7.288798488731946E-4 14-15 0.0 0.0 0.0 8.575057045566996E-5 9.003809897845345E-4 16-17 0.0 0.0 0.0 1.2862585568350492E-4 0.0011147574159237094 18-19 0.0 0.0 0.0 1.929387835252574E-4 0.0011147574159237094 20-21 0.0 0.0 0.0 3.2156463920876235E-4 0.001307696199448967 22-23 0.0 0.0 0.0 5.145034227340197E-4 0.0013720091272907194 24-25 0.0 0.0 0.0 7.503174914871121E-4 0.0013720091272907194 26-27 0.0 0.0 0.0 0.0014577596977463892 0.0014791973403603066 28-29 0.0 0.0 0.0 0.003730149814821643 0.0015435102682020592 30-31 0.0 0.0 0.0 0.011340512942762351 0.0015863855534298942 32-33 0.0 0.0 0.0 0.028211937679915415 0.0017150114091133992 34-35 0.0 0.0 0.0 0.05625237421891949 0.001843637264796904 36-37 0.0 0.0 0.0 0.10452994538546168 0.001843637264796904 38-39 0.0 0.0 0.0 0.18069788959271052 0.0019079501926386566 40-41 0.0 0.0 0.0 0.2587523463499841 0.001929387835252574 42-43 0.0 0.0 0.0 0.3436882863863252 0.001929387835252574 44-45 0.0 0.0 0.0 0.4491186127615714 0.002015138405708244 46-47 0.0 0.0 0.0 0.5738428174893433 0.002100888976163914 48-49 0.0 0.0 0.0 0.7088356530291817 0.002315265402303089 50-51 0.0 0.0 0.0 0.8585132737595537 0.0024438912579865936 52-53 0.0 0.0 0.0 1.002874359121674 0.0027011429693536036 54-55 0.0 0.0 0.0 1.159069023206677 0.0027011429693536036 56-57 0.0 0.0 0.0 1.3417177382772538 0.002829768825037109 58-59 0.0 0.0 0.0 1.5378721681945988 0.002894081752878861 60-61 0.0 0.0 0.0 1.7591086399702274 0.0029155193954927784 62-63 0.0 0.0 0.0 1.976979401855471 0.0030012699659484484 64-65 0.0 0.0 0.0 2.2056118603329007 0.0030870205364041184 66-67 0.0 0.0 0.0 2.469016175130105 0.003129895821631953 68-69 0.0 0.0 0.0 2.7605466770367686 0.0031727711068597884 70-71 0.0 0.0 0.0 3.0733861957016666 0.0032156463920876236 72-73 2.143764261391749E-5 0.0 0.0 3.3982736695155866 0.0034943357460685506 74-75 4.287528522783498E-5 0.0 0.0 3.727920299989796 0.0037944627426633954 76-77 4.287528522783498E-5 0.0 0.0 4.079218949504062 0.003901650955732983 78-79 4.287528522783498E-5 0.0 0.0 4.4788594831127115 0.003901650955732983 80-81 4.287528522783498E-5 2.143764261391749E-5 0.0 4.903753559720556 0.003944526240960818 82-83 4.287528522783498E-5 4.287528522783498E-5 0.0 5.335443369037012 0.004373279093239168 84-85 4.287528522783498E-5 4.287528522783498E-5 0.0 5.771142017522272 0.004373279093239168 86-87 4.287528522783498E-5 4.287528522783498E-5 0.0 6.226992050064613 0.004501904948922672 88-89 4.287528522783498E-5 4.287528522783498E-5 0.0 6.709810637015263 0.004630530804606178 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6505 0.0 35.439655 1 GTATCAA 9840 0.0 33.42767 1 TAAGGTG 1440 0.0 26.72354 5 GTAAGGT 1340 0.0 26.590588 4 ATCAACG 12260 0.0 26.583069 3 TATCAAC 12320 0.0 26.3895 2 TCAACGC 12545 0.0 26.017017 4 CAACGCA 12650 0.0 25.801067 5 GTACATG 10245 0.0 25.657139 1 TACATGG 10150 0.0 25.568918 2 AACGCAG 12945 0.0 25.237345 6 ACATGGG 10365 0.0 24.201155 3 AGGTAAG 1355 0.0 23.85368 2 AAGGTAA 1450 0.0 21.963516 1 ACGCAGA 14860 0.0 21.953068 7 CGCAGAG 15045 0.0 21.619997 8 GAGTACT 7370 0.0 21.584131 12-13 GGACCGA 890 0.0 21.341614 6 CATGGGG 5535 0.0 21.114895 4 GTACTTT 7550 0.0 20.943754 14-15 >>END_MODULE