FastQCFastQC Report
Thu 26 May 2016
SRR938554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330979
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68060.2919803224310472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.22205262252469887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.1598040994792317No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36870.15817388316239658No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33710.14461734747503088No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33510.14375933888722292No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30350.13020280319985722No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28350.12162271732177768No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27420.11763297738847069No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25450.10918159279856231No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25270.10840938506953515No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25150.10789457991685039No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG23780.10201722109036589No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92250.034.5659451
GGTATCA59900.034.2444731
TAAGGTG14250.028.681275
ATCAACG115950.027.33823
TATCAAC115950.027.2562262
GTAAGGT14000.027.1566834
GTACATG101900.026.8555281
TCAACGC119150.026.683754
CAACGCA119950.026.4265465
AGGTAAG13650.026.11222
AACGCAG123900.025.885356
TACATGG102800.025.842442
GGTAAGG15100.024.5489233
ACATGGG105450.024.2015133
AAGGTAA14700.023.6344531
ACGCAGA141150.022.4828787
CGCAGAG142150.022.1576128
GGACCGA8900.021.3546056
GAGTACT67600.021.0090412-13
GTGTAAG8650.020.9077591
Produced by FastQC (version 0.11.5)