##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938554_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2330979 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.058657328101198 31.0 31.0 34.0 28.0 34.0 2 31.139871701975864 31.0 31.0 34.0 27.0 34.0 3 31.20927172660071 31.0 31.0 34.0 27.0 34.0 4 34.84676996232055 37.0 35.0 37.0 32.0 37.0 5 34.8623406731678 37.0 35.0 37.0 32.0 37.0 6 34.898844648536084 37.0 35.0 37.0 32.0 37.0 7 34.823933634751754 37.0 35.0 37.0 32.0 37.0 8 34.77680880007928 37.0 35.0 37.0 32.0 37.0 9 36.28097078523659 39.0 35.0 39.0 32.0 39.0 10-11 36.12912407190284 39.0 35.0 39.0 31.0 39.0 12-13 35.98437266058596 38.0 35.0 39.0 30.0 39.0 14-15 36.96561573484789 39.0 36.0 41.0 31.0 41.0 16-17 36.825651367944545 39.0 36.0 41.0 30.5 41.0 18-19 36.817090801761836 39.0 36.0 41.0 30.5 41.0 20-21 36.89585491761187 39.0 36.0 41.0 30.0 41.0 22-23 36.819537413249975 39.0 36.0 41.0 30.0 41.0 24-25 36.69369822722555 39.0 36.0 41.0 30.0 41.0 26-27 36.50536834523177 39.0 36.0 41.0 30.0 41.0 28-29 36.32660461548559 39.0 35.0 40.5 29.5 41.0 30-31 36.10439540639362 39.0 35.0 40.0 29.0 41.0 32-33 35.83530546607241 38.0 35.0 40.0 27.5 41.0 34-35 35.583483377585125 38.0 34.5 40.0 27.0 41.0 36-37 35.297197229147066 38.0 34.0 40.0 26.0 41.0 38-39 35.03920670241988 38.0 34.0 40.0 25.0 41.0 40-41 34.980651477340636 38.0 34.0 40.0 25.0 41.0 42-43 34.790216256774514 38.0 33.5 40.0 24.5 41.0 44-45 34.504041005946426 38.0 33.0 40.0 24.0 41.0 46-47 34.137917372914984 38.0 33.0 40.0 23.0 41.0 48-49 33.76749854889297 38.0 33.0 40.0 21.5 41.0 50-51 33.004664134683324 37.0 31.5 39.5 20.0 40.5 52-53 33.05308263180406 37.0 32.0 39.5 20.0 40.0 54-55 33.17741708526761 37.0 32.0 40.0 19.5 41.0 56-57 32.86706658446944 37.0 31.5 40.0 17.5 41.0 58-59 32.53110731585312 36.0 31.0 39.5 14.5 41.0 60-61 32.07259889514234 36.0 31.0 39.0 12.0 41.0 62-63 31.55112637222386 35.0 30.0 39.0 8.0 40.0 64-65 31.054689896391174 35.0 29.5 38.5 7.0 40.0 66-67 30.5123684082954 35.0 29.0 38.0 3.5 40.0 68-69 29.952487130943695 34.0 28.5 37.5 2.0 39.5 70-71 29.323702186935186 34.0 28.0 36.5 2.0 39.0 72-73 28.78339637551432 34.0 26.5 36.0 2.0 39.0 74-75 28.19383743911893 33.5 26.0 35.5 2.0 38.5 76-77 27.48431989305781 33.0 25.5 35.0 2.0 37.5 78-79 26.729408115645832 32.0 24.0 35.0 2.0 37.0 80-81 26.09795626644427 32.0 22.5 35.0 2.0 36.0 82-83 25.45236615173281 31.5 20.0 34.5 2.0 36.0 84-85 24.827434953296446 31.0 18.0 34.0 2.0 35.0 86-87 24.180217196293917 31.0 13.5 34.0 2.0 35.0 88-89 23.40768599802915 30.0 6.0 34.0 2.0 35.0 90-91 22.599975589655678 29.5 2.0 34.0 2.0 35.0 92-93 21.768782129740337 29.0 2.0 34.0 2.0 35.0 94-95 20.818282146686006 29.0 2.0 34.0 2.0 35.0 96-97 19.601033943248737 26.5 2.0 33.5 2.0 35.0 98-99 18.122752071125483 24.5 2.0 33.0 2.0 34.5 100-101 14.693823710981523 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5830878146504617 1101 2 0.7098430061938856 1101 3 0.7754702319063149 1101 4 0.6348193559177133 1101 5 0.5161039380475501 1101 6 0.510367862430833 1101 7 0.542034108628414 1101 8 0.5983826932034617 1101 9 0.6283962881595428 1101 10-11 0.6346666657002515 1101 12-13 0.6365822542073616 1101 14-15 0.7596079872538795 1101 16-17 0.8830643008689165 1101 18-19 0.7532215205512784 1101 20-21 0.7025682576660728 1101 22-23 0.8124394617647326 1101 24-25 0.8976219425776861 1101 26-27 0.9068441678616779 1101 28-29 0.9088448932577435 1101 30-31 0.8709336696000207 1101 32-33 1.0103009388540656 1101 34-35 0.964741010299285 1101 36-37 0.9308587478403183 1101 38-39 0.8861180160664617 1101 40-41 0.7792506006834259 1101 42-43 0.9103730419381435 1101 44-45 1.1425153452318426 1101 46-47 1.176955863905441 1101 48-49 1.2087843158346558 1101 50-51 1.1877711711322547 1101 52-53 1.2940787597835524 1101 54-55 1.259408468292655 1101 56-57 1.337911825391025 1101 58-59 1.2133882349354579 1101 60-61 1.360911986231521 1101 62-63 1.2563237634047724 1101 64-65 1.3506006407748288 1101 66-67 1.1144273759236256 1101 68-69 1.1351586745100484 1101 70-71 1.4575239402396853 1101 72-73 1.2890473695807962 1101 74-75 1.234863535528774 1101 76-77 1.4575143939573785 1101 78-79 1.4562894381914404 1101 80-81 1.6933897983981545 1101 82-83 1.5074022099460898 1101 84-85 1.584990222803512 1101 86-87 1.6079569963838942 1101 88-89 1.794239679560011 1101 90-91 1.7269842482842783 1101 92-93 1.7918088771238416 1101 94-95 1.7952716399112312 1101 96-97 2.0056779577104535 1101 98-99 1.7398580678147795 1101 100-101 1.308523956902535 1102 1 0.6606772487091703 1102 2 0.6459867400199073 1102 3 0.5843032225164606 1102 4 0.642719098208552 1102 5 0.6538934339288289 1102 6 0.6453952978745008 1102 7 0.6252198090798444 1102 8 0.6956749529680053 1102 9 0.8132473028689731 1102 10-11 0.8012733070405105 1102 12-13 0.8593994637530571 1102 14-15 0.7069809022532212 1102 16-17 0.8433902144067389 1102 18-19 0.9018310212333063 1102 20-21 0.8056777294707018 1102 22-23 0.8553453220338341 1102 24-25 0.8099832711220429 1102 26-27 0.9057930690720113 1102 28-29 0.6997404167626939 1102 30-31 0.7446836189926245 1102 32-33 0.8115645982934581 1102 34-35 0.9120232637108003 1102 36-37 0.7858894298322738 1102 38-39 0.9559077713645934 1102 40-41 0.9556172257832785 1102 42-43 1.0666011860196463 1102 44-45 0.9812513097147644 1102 46-47 0.8930467511398845 1102 48-49 0.8850349516649985 1102 50-51 0.9957057965483145 1102 52-53 0.9541777219434167 1102 54-55 0.737586272071816 1102 56-57 0.595880883022815 1102 58-59 0.7960869531436998 1102 60-61 0.70820429396516 1102 62-63 0.6920606233747435 1102 64-65 0.7615549398513792 1102 66-67 0.7576618436748674 1102 68-69 0.8198947552700524 1102 70-71 0.773787727276865 1102 72-73 0.9934552880118481 1102 74-75 0.8287215919093427 1102 76-77 1.027043197383673 1102 78-79 0.8882212936801288 1102 80-81 1.0379236913453376 1102 82-83 1.1007038248352323 1102 84-85 1.070726228923597 1102 86-87 1.0547877646818513 1102 88-89 1.3592555506144812 1102 90-91 1.4460691744953422 1102 92-93 1.4949272903676096 1102 94-95 1.5274743864680573 1102 96-97 1.6571773084967347 1102 98-99 1.62334046670696 1102 100-101 1.4198927473272906 1103 1 0.5920586607685152 1103 2 0.7255259961715197 1103 3 0.4977017414349021 1103 4 0.45841303026835334 1103 5 0.5011702009868344 1103 6 0.5472325776562315 1103 7 0.3825609743738383 1103 8 0.47477105640565753 1103 9 0.3915606675701966 1103 10-11 0.40928144479422457 1103 12-13 0.4666067487965577 1103 14-15 0.6303721422094242 1103 16-17 0.7022233073946467 1103 18-19 0.5709314557091147 1103 20-21 0.5221767970738398 1103 22-23 0.6613950926334269 1103 24-25 0.7790991312131936 1103 26-27 0.8014082822885058 1103 28-29 0.6678506453918232 1103 30-31 0.747091275734121 1103 32-33 0.7645548501203336 1103 34-35 0.8805094917140011 1103 36-37 0.8691563267213098 1103 38-39 0.8487016914473031 1103 40-41 0.9470944806022032 1103 42-43 0.891064543323246 1103 44-45 0.8845531886362892 1103 46-47 0.9205983463005367 1103 48-49 0.8412673637751453 1103 50-51 0.7697652140904268 1103 52-53 0.7924767623292368 1103 54-55 0.8885313876582472 1103 56-57 0.770401326227443 1103 58-59 0.7435542970047706 1103 60-61 0.9740825732525948 1103 62-63 1.1364652780541746 1103 64-65 0.9042918481946742 1103 66-67 0.8436561265561799 1103 68-69 0.9218722561201282 1103 70-71 0.9257090877864727 1103 72-73 1.0476176455798374 1103 74-75 0.9849623101200606 1103 76-77 1.0327424245825974 1103 78-79 1.1604513296522185 1103 80-81 0.9793415797936653 1103 82-83 1.005779834925665 1103 84-85 1.094046069787609 1103 86-87 1.322688523837197 1103 88-89 1.4332906246073733 1103 90-91 1.3183287638699106 1103 92-93 1.2135077963203784 1103 94-95 0.9958029283432275 1103 96-97 1.1552919349094672 1103 98-99 1.4805352369783904 1103 100-101 1.1364339016453684 1104 1 0.5425083492089051 1104 2 0.5491597576464216 1104 3 0.5689985846443477 1104 4 0.5902582905879399 1104 5 0.5699723731095858 1104 6 0.5969325907152978 1104 7 0.4023376749457128 1104 8 0.4562319590101467 1104 9 0.5550102855836414 1104 10-11 0.5173258477453615 1104 12-13 0.6182669313474491 1104 14-15 0.851816581188011 1104 16-17 0.6232693032688275 1104 18-19 0.7007158430813831 1104 20-21 0.7868426778371216 1104 22-23 0.8945139481920066 1104 24-25 0.8959665441695606 1104 26-27 0.7642509014206382 1104 28-29 0.8412926615418854 1104 30-31 0.663794424030975 1104 32-33 0.7014170925355501 1104 34-35 0.7684465059582948 1104 36-37 0.7320434670086016 1104 38-39 0.8799417931876974 1104 40-41 0.7865635994478524 1104 42-43 0.8811978696728673 1104 44-45 0.761571902739334 1104 46-47 0.7673371009043919 1104 48-49 0.9115483248233787 1104 50-51 0.7661074366728471 1104 52-53 0.8548556607173055 1104 54-55 0.9360197645577841 1104 56-57 0.9695986359888735 1104 58-59 0.9455501931310408 1104 60-61 0.9344421116623565 1104 62-63 0.9116832565318305 1104 64-65 0.8525086662202952 1104 66-67 0.7756928844676203 1104 68-69 0.8341545209516354 1104 70-71 0.7613814333187676 1104 72-73 0.7974157432029081 1104 74-75 0.7192529426637506 1104 76-77 1.0049006884415768 1104 78-79 1.1542365939992294 1104 80-81 1.1008103673849305 1104 82-83 1.0146653975077946 1104 84-85 0.9634351390300395 1104 86-87 1.0330262971353292 1104 88-89 1.073712666047438 1104 90-91 1.007733189017923 1104 92-93 1.0204377193851322 1104 94-95 0.8484154318513006 1104 96-97 0.6930013179375614 1104 98-99 0.6536997037172263 1104 100-101 0.7363870801125589 1105 1 0.43868105416401093 1105 2 0.4033653625967766 1105 3 0.3288849455353535 1105 4 0.4708027459931543 1105 5 0.3747724023032646 1105 6 0.33174938431309897 1105 7 0.39465912846668516 1105 8 0.3586983689593808 1105 9 0.44275889014086545 1105 10-11 0.47427192508936145 1105 12-13 0.4437380469194565 1105 14-15 0.5840267236609478 1105 16-17 0.5568291804729029 1105 18-19 0.5748894701378475 1105 20-21 0.6144375154886603 1105 22-23 0.6849865192244735 1105 24-25 0.5738036234903774 1105 26-27 0.6002814966693322 1105 28-29 0.6848044756065192 1105 30-31 0.7200649920187203 1105 32-33 0.6719289398823207 1105 34-35 0.5933314671406649 1105 36-37 0.8223770470578273 1105 38-39 0.6186180489425936 1105 40-41 0.6093845605788673 1105 42-43 0.6219073775042361 1105 44-45 0.7105513825207694 1105 46-47 0.7767305227637351 1105 48-49 0.6340492029145892 1105 50-51 0.48993132240931203 1105 52-53 0.2962648877115228 1105 54-55 0.338661032496141 1105 56-57 0.5272159483827181 1105 58-59 0.5770906210583746 1105 60-61 0.6602362330212301 1105 62-63 0.6775974875900275 1105 64-65 0.7093580057047966 1105 66-67 0.7968581347282608 1105 68-69 0.6706760701292218 1105 70-71 0.6287687964754944 1105 72-73 0.5491143576349629 1105 74-75 0.5204268226307533 1105 76-77 0.6493072854702717 1105 78-79 0.648456397080416 1105 80-81 0.6673267688108915 1105 82-83 0.7071167189864873 1105 84-85 0.550272572966243 1105 86-87 0.746050547995555 1105 88-89 0.7769546683217747 1105 90-91 0.7314684631454114 1105 92-93 0.8427518457650542 1105 94-95 0.890868417437428 1105 96-97 0.6356878529769894 1105 98-99 0.9209668456709998 1105 100-101 0.6653996359298482 1106 1 0.3165714283144112 1106 2 0.41609716640465066 1106 3 0.20195126693878507 1106 4 0.4884526532658029 1106 5 0.49273963503318186 1106 6 0.1981800530370066 1106 7 0.15041773739498154 1106 8 0.327067031792879 1106 9 0.502328617262279 1106 10-11 0.327636119265307 1106 12-13 0.32888048048926777 1106 14-15 0.5957530170955252 1106 16-17 0.29714441184516005 1106 18-19 0.07432266890928929 1106 20-21 0.3639480772178061 1106 22-23 0.45489626549228745 1106 24-25 0.6058730039558284 1106 26-27 0.39342801253967963 1106 28-29 0.3761113760827186 1106 30-31 0.5011070140458926 1106 32-33 0.6092566210726957 1106 34-35 0.5672821649220836 1106 36-37 0.5208060005629847 1106 38-39 0.4306259785001032 1106 40-41 0.617266676576854 1106 42-43 0.21525845784734798 1106 44-45 0.4566972590126994 1106 46-47 0.134053253390654 1106 48-49 0.37192610700466133 1106 50-51 0.09954393832266106 1106 52-53 0.3296817735940536 1106 54-55 0.28087935101246586 1106 56-57 -0.1953251122463655 1106 58-59 -0.4109006836614526 1106 60-61 0.3751671756574453 1106 62-63 0.24061704497902525 1106 64-65 0.492123319866657 1106 66-67 -0.24328362716961394 1106 68-69 -0.4199742564598168 1106 70-71 -0.24023919483920864 1106 72-73 0.3499780262088876 1106 74-75 0.18062557426977222 1106 76-77 0.1966754717246495 1106 78-79 0.15598794527008764 1106 80-81 0.2438884643339314 1106 82-83 0.19511651324013357 1106 84-85 0.16031590787485328 1106 86-87 0.3247550185008343 1106 88-89 0.16617790427571677 1106 90-91 0.3769615943634754 1106 92-93 0.2965801843949123 1106 94-95 0.30485620615856845 1106 96-97 0.29264160515950977 1106 98-99 0.1059845829705317 1106 100-101 -0.34994161752818087 1107 1 0.4187499598138693 1107 2 0.25568269847942204 1107 3 0.3215855768285891 1107 4 0.4537843985044532 1107 5 0.4448613199616318 1107 6 0.3487897104964617 1107 7 0.32482141914811535 1107 8 0.4082912584120777 1107 9 0.4464330766686757 1107 10-11 0.4505264689809749 1107 12-13 0.4032623270276616 1107 14-15 0.41418823287767026 1107 16-17 0.5126866002848587 1107 18-19 0.5303400398907314 1107 20-21 0.5504778859550257 1107 22-23 0.6144536319338272 1107 24-25 0.5323765517297829 1107 26-27 0.5103047908295792 1107 28-29 0.6472105695984851 1107 30-31 0.7479336512467114 1107 32-33 0.7199730639841277 1107 34-35 0.7119448654831828 1107 36-37 0.5666783785434362 1107 38-39 0.5662040719254691 1107 40-41 0.45927789064740665 1107 42-43 0.5064464566001803 1107 44-45 0.5720356249363334 1107 46-47 0.5368391756618607 1107 48-49 0.6023411913384891 1107 50-51 0.5125944499091872 1107 52-53 0.4725592537417782 1107 54-55 0.595687214168457 1107 56-57 0.48385092557046505 1107 58-59 0.5292797153277675 1107 60-61 0.4433672429731814 1107 62-63 0.4810539873575408 1107 64-65 0.4353359436538966 1107 66-67 0.34369978002403556 1107 68-69 0.48982893960247154 1107 70-71 0.47264151085405715 1107 72-73 0.35172304960632417 1107 74-75 0.46749512525035186 1107 76-77 0.563670394996457 1107 78-79 0.4683762415949886 1107 80-81 0.4561667040014221 1107 82-83 0.5550414092334144 1107 84-85 0.5606257591931865 1107 86-87 0.6968183533431187 1107 88-89 0.8260667941792725 1107 90-91 0.8665576608286294 1107 92-93 0.783276439761579 1107 94-95 0.6053591499173159 1107 96-97 0.7767754434478107 1107 98-99 0.822571241540178 1107 100-101 0.6265724809432562 1108 1 0.2841691507335007 1108 2 0.3365968400363606 1108 3 0.34873602187676767 1108 4 0.24429303601187513 1108 5 0.3540807442648912 1108 6 0.4016078563303864 1108 7 0.3222392272440544 1108 8 0.19843587658053252 1108 9 0.2107348920745622 1108 10-11 0.29196761286917905 1108 12-13 0.40446249846695537 1108 14-15 0.44452714034414953 1108 16-17 0.3467973971048792 1108 18-19 0.39174457622070236 1108 20-21 0.3908157267452381 1108 22-23 0.45144911333895976 1108 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70-71 -1.08302319446166 2316 72-73 -1.1159313833679185 2316 74-75 -0.892498399348149 2316 76-77 -0.762250008405001 2316 78-79 -0.8368964611091627 2316 80-81 -0.9052262565414431 2316 82-83 -0.7870470950558719 2316 84-85 -0.8926453219848014 2316 86-87 -1.0270261754777685 2316 88-89 -1.0792010252158981 2316 90-91 -1.1222455137235805 2316 92-93 -1.1470236897464474 2316 94-95 -1.1508352940811655 2316 96-97 -1.2050593160793248 2316 98-99 -1.258781006922586 2316 100-101 -0.9505025240069465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7123.0 3 2572.0 4 2817.0 5 3178.0 6 3609.0 7 4788.0 8 5936.0 9 7588.0 10 9687.0 11 11736.0 12 14263.0 13 16730.0 14 18953.0 15 21978.0 16 25213.0 17 29230.0 18 28093.0 19 27034.0 20 28036.0 21 30805.0 22 34024.0 23 37649.0 24 42482.0 25 47511.0 26 53369.0 27 61197.0 28 70517.0 29 80759.0 30 93485.0 31 109486.0 32 129502.0 33 155376.0 34 192277.0 35 238207.0 36 281326.0 37 274343.0 38 123877.0 39 6223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44582457974164 17.85272819796928 13.99235128063184 23.70909594165724 2 15.900791313917614 19.505098300452055 39.354604437470066 25.239505948160268 3 19.777922281382857 23.24381978826645 28.77880861785163 28.19944931249906 4 13.129801662215376 15.685794763360523 35.52897373095325 35.655429843470856 5 14.293422921127597 37.07988550254048 33.14060109449131 15.486090481840614 6 33.80731845738684 34.76383168526804 16.729300875161737 14.69954898218338 7 30.313745113960383 31.971264851242946 19.304083759207728 18.410906275588943 8 27.69779356290625 33.747621430429035 19.070827943911677 19.48375706275304 9 27.216188133663348 16.355858769280555 19.210490286922376 37.217462810133725 10-11 25.88832705173983 25.873562821264183 26.405869124968266 21.832241002027722 12-13 26.83852593468112 23.109887100941354 26.83135869420604 23.22022827017149 14-15 23.241960641513586 24.594708785352672 25.156476280129286 27.00685429300445 16-17 23.071217062913053 27.905265642434124 25.938760621405887 23.084756673246932 18-19 23.22499847639354 26.13963617136152 28.16056065284176 22.474804699403176 20-21 23.594051299440604 25.370849478646335 28.26482641396457 22.770272807948484 22-23 23.35071592394525 25.293160870907922 28.248988173557503 23.10713503158933 24-25 23.17312010110958 25.539720454684 28.300550281322636 22.986609162883788 26-27 22.544932354922626 25.96016262204111 28.178412939196157 23.316492083840103 28-29 22.679326750776323 26.502117048193156 28.079183825880566 22.73937237514995 30-31 22.6459233491953 25.814682003809963 27.942032431894138 23.5973622151006 32-33 22.413260273690376 25.903559382707254 27.942308864419307 23.740871479183067 34-35 23.436721692712453 26.014137413669257 27.641912278672343 22.907228614945947 36-37 23.48720768305159 25.4272421615847 27.706847055536276 23.378703099827437 38-39 22.83321249941264 25.856899881497647 27.788833658358342 23.521053960731372 40-41 23.252905958846267 25.72779850874362 27.872187587968334 23.147107944441775 42-43 22.948475094246596 25.714393602452397 27.61711176205682 23.720019541244188 44-45 22.91369345572183 25.994027586535317 27.837899234346118 23.254379723396728 46-47 23.462164380470128 26.364155905003035 27.00058822847978 23.173091486047056 48-49 23.175927640133096 26.328618020160555 27.03118473771951 23.464269601986835 50-51 23.33009263450366 26.475079297726552 27.085713623763446 23.109114444006345 52-53 23.688485747401963 26.114228013680574 27.018679176514176 23.178607062403287 54-55 22.831521011605037 26.383246836159934 27.338191076374947 23.44704107586008 56-57 23.054903291657567 26.596628325991702 27.05913622573818 23.289332156612556 58-59 23.079601365679732 25.91395531185273 27.105975331524867 23.900467990942666 60-61 22.615781192949274 26.329175946738996 27.603422303322894 23.451620556988832 62-63 22.78392493164625 26.2891755034238 27.66944286064244 23.257456704287506 64-65 22.410038329759495 26.378073695762787 27.397733678961878 23.814154295515838 66-67 22.494386254840435 26.424844458287534 27.248136545502437 23.83263274136959 68-69 23.044239723076977 25.89754139858484 27.552412178709396 23.50580669962879 70-71 22.822753430835533 26.212394476677698 27.12716952260311 23.837682569883654 72-73 22.853737061147463 26.17210781808562 27.213502276997755 23.76065284376916 74-75 22.733556077767027 26.707469533551876 27.027897978672577 23.531076410008524 76-77 22.96489707164171 26.19904667082426 27.14791361899742 23.688142638536615 78-79 23.07163451875086 26.442247979453136 27.080551374349408 23.405566127446594 80-81 23.18253427537339 26.573839854335024 26.82997200994668 23.41365386034491 82-83 23.028337301842676 26.541347577064162 26.906085762704592 23.52422935838857 84-85 22.98174858598359 26.537602743707744 26.930912424831945 23.549736245476723 86-87 23.240364951650193 26.556387158002632 26.674716923494746 23.528530966852436 88-89 23.015750915515124 26.651869942458852 26.479999639522962 23.852379502503062 90-91 22.92646646379866 26.59620147149044 26.70197007707654 23.775361987634362 92-93 23.207463696567196 26.072775504231625 26.926074062821964 23.793686736379215 94-95 22.967298944296626 25.980023173976484 27.09921895116299 23.9534589305639 96-97 23.251639085004964 25.606805856030036 27.093494687592134 24.048060371372866 98-99 22.986110679031324 25.992835318528385 26.574995188717075 24.44605881372322 100-101 23.47416448886529 25.25201500320442 27.016227412290878 24.257593095639415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 469.0 1 332.5 2 285.5 3 387.5 4 337.5 5 328.5 6 577.0 7 1244.0 8 1962.0 9 2252.0 10 1982.5 11 1623.5 12 1666.5 13 1837.0 14 1981.0 15 2068.0 16 2161.0 17 2337.5 18 2548.0 19 2823.0 20 3147.0 21 3513.5 22 4184.0 23 5023.0 24 5909.0 25 7351.5 26 9330.0 27 11788.0 28 14184.5 29 16757.5 30 19757.5 31 24658.0 32 32430.5 33 35223.0 34 34815.0 35 37737.5 36 42446.5 37 47507.0 38 51808.5 39 54985.0 40 58338.5 41 64729.5 42 70180.0 43 73575.0 44 77204.0 45 82320.0 46 85481.0 47 88760.0 48 93973.0 49 93096.5 50 89958.0 51 88202.0 52 87298.5 53 88802.0 54 90227.5 55 85913.0 56 78561.5 57 73304.5 58 69002.5 59 61043.0 60 49924.0 61 41848.0 62 35397.0 63 26907.5 64 19762.0 65 15677.0 66 12168.0 67 9600.5 68 7456.5 69 5376.5 70 3957.0 71 2938.0 72 2238.0 73 1780.0 74 1340.0 75 1043.0 76 780.5 77 497.5 78 304.0 79 175.0 80 103.0 81 62.0 82 45.0 83 27.5 84 19.0 85 16.0 86 7.0 87 4.5 88 8.0 89 7.0 90 2.0 91 2.0 92 1.5 93 1.0 94 2.0 95 2.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18511535281956637 2 0.05075120796884056 3 0.049936099810423004 4 0.056499865507153864 5 0.06413614193864466 6 0.06782557886621887 7 0.037366273999036456 8 0.03419164222414702 9 0.04187081908502822 10-11 0.04390858948107212 12-13 0.040026100621241116 14-15 0.03575750789689654 16-17 0.03341943449511987 18-19 0.04208532123198021 20-21 0.05261737664732286 22-23 0.04965724701938541 24-25 0.06971319775939637 26-27 0.055019800693185135 28-29 0.04650406545919118 30-31 0.0556418569193459 32-33 0.051180212262744544 34-35 0.05163066677134372 36-37 0.048198632420111895 38-39 0.02769222717150176 40-41 0.025525755487286673 42-43 0.02245837478587323 44-45 0.004096991006782987 46-47 0.010810908206380237 48-49 0.010274652839000266 50-51 0.00883748845442194 52-53 0.00980274811570589 54-55 0.011454414647236203 56-57 0.008923289313202735 58-59 0.011647466579492994 60-61 0.011497315076626602 62-63 0.003861038645135799 64-65 0.006563765696730858 66-67 0.003989739933306992 68-69 0.009566795754058702 70-71 0.024453244752526727 72-73 0.021364413836418086 74-75 0.021686167056846072 76-77 0.01306318074937612 78-79 0.004826298306419749 80-81 0.006477964837950063 82-83 0.005534155391361312 84-85 0.005083700882762135 86-87 0.008773137810336343 88-89 0.03146746495785676 90-91 0.03828863323093001 92-93 0.02368103702349957 94-95 0.03341943449511987 96-97 0.03723757271086526 98-99 0.02228677306831164 100-101 0.12625596369594064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2330979.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.625590671815104 #Duplication Level Percentage of deduplicated Percentage of total 1 75.77698407754974 37.604775941771344 2 12.81042918833563 12.714502304612335 3 4.553609696315537 6.779267126056893 4 2.1346243586068825 4.23727978633244 5 1.1729519530427925 2.9104216749703857 6 0.7162904636256636 2.1327802410007113 7 0.48245402790446396 1.6759446274728775 8 0.3601015649823594 1.429620229127568 9 0.27473089998067723 1.2270314868606418 >10 1.5258538369043735 13.97019190572749 >50 0.11820186690737534 4.0075251769740134 >100 0.06584730913856518 6.056320456530316 >500 0.004265022841773253 1.5411023105363202 >1k 0.0034816512994066172 3.195677230797853 >5k 1.7408256497033084E-4 0.5175595012289294 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6806 0.2919803224310472 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5176 0.22205262252469887 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3725 0.1598040994792317 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3687 0.15817388316239658 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3371 0.14461734747503088 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3351 0.14375933888722292 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3035 0.13020280319985722 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2835 0.12162271732177768 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2742 0.11763297738847069 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2545 0.10918159279856231 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2527 0.10840938506953515 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2515 0.10789457991685039 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2378 0.10201722109036589 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.290042939039777E-5 0.0 3 0.0 0.0 0.0 8.580085878079554E-5 0.0 4 0.0 0.0 0.0 8.580085878079554E-5 0.0 5 0.0 0.0 0.0 8.580085878079554E-5 4.290042939039777E-5 6 0.0 0.0 0.0 8.580085878079554E-5 4.290042939039777E-5 7 0.0 0.0 0.0 8.580085878079554E-5 4.290042939039777E-5 8 0.0 0.0 0.0 8.580085878079554E-5 4.290042939039777E-5 9 0.0 0.0 0.0 8.580085878079554E-5 4.290042939039777E-5 10-11 0.0 0.0 0.0 8.580085878079554E-5 4.290042939039777E-5 12-13 0.0 0.0 0.0 8.580085878079554E-5 6.864068702463642E-4 14-15 0.0 0.0 0.0 8.580085878079554E-5 7.722077290271598E-4 16-17 4.290042939039777E-5 0.0 0.0 1.9305193225678996E-4 7.936579437223587E-4 18-19 4.290042939039777E-5 0.0 0.0 2.5740257634238663E-4 9.009090171983532E-4 20-21 4.290042939039777E-5 0.0 0.0 3.4320343512318216E-4 0.0010939609494551431 22-23 4.290042939039777E-5 0.0 0.0 5.148051526847732E-4 0.0011797618082359388 24-25 4.290042939039777E-5 0.0 0.0 9.652596612839498E-4 0.0012012120229311376 26-27 4.290042939039777E-5 0.0 0.0 0.0015229652433591209 0.0012226622376263364 28-29 4.290042939039777E-5 0.0 0.0 0.0035607356394030147 0.0012441124523215354 30-31 4.290042939039777E-5 0.0 0.0 0.011797618082359386 0.0012655626670167342 32-33 4.290042939039777E-5 0.0 0.0 0.03039495422309682 0.0013728137404927286 34-35 4.290042939039777E-5 0.0 0.0 0.059159692129358524 0.0013728137404927286 36-37 4.290042939039777E-5 0.0 0.0 0.10502025114769374 0.0013728137404927286 38-39 4.290042939039777E-5 0.0 0.0 0.180331954942537 0.0014157141698831264 40-41 4.290042939039777E-5 0.0 0.0 0.2626364287280152 0.0014157141698831264 42-43 4.290042939039777E-5 0.0 0.0 0.3472146252711843 0.0014371643845783252 44-45 4.290042939039777E-5 0.0 0.0 0.4521061751307069 0.0015444154580543197 46-47 4.290042939039777E-5 0.0 0.0 0.5735358405202278 0.001673116746225513 48-49 4.290042939039777E-5 0.0 0.0 0.7068918252802793 0.001866168678482303 50-51 4.290042939039777E-5 0.0 0.0 0.8569146268585002 0.001994869966653496 52-53 4.290042939039777E-5 0.0 0.0 1.0012745717571887 0.0021021210401294906 54-55 4.290042939039777E-5 0.0 0.0 1.1612073725245917 0.0021021210401294906 56-57 4.290042939039777E-5 0.0 0.0 1.3440704528011622 0.0021021210401294906 58-59 4.290042939039777E-5 0.0 0.0 1.5368435322669145 0.0021021210401294906 60-61 4.290042939039777E-5 0.0 0.0 1.7523109388801874 0.0021021210401294906 62-63 4.290042939039777E-5 0.0 0.0 1.9700091678217606 0.0021450214695198882 64-65 4.290042939039777E-5 0.0 0.0 2.197188391658612 0.0021879218989102863 66-67 4.290042939039777E-5 0.0 0.0 2.463900361178715 0.0021879218989102863 68-69 4.290042939039777E-5 0.0 0.0 2.7516549913148083 0.0021879218989102863 70-71 4.290042939039777E-5 0.0 0.0 3.0606882344285387 0.0021879218989102863 72-73 4.290042939039777E-5 0.0 0.0 3.3854444849138496 0.0022951729723862807 74-75 4.290042939039777E-5 0.0 0.0 3.7073693070593943 0.0024453244752526727 76-77 4.290042939039777E-5 0.0 0.0 4.053168218160695 0.0024882249046430708 78-79 4.290042939039777E-5 0.0 0.0 4.4496539865867515 0.0024882249046430708 80-81 4.290042939039777E-5 0.0 0.0 4.865230446091536 0.0025096751193382696 82-83 4.290042939039777E-5 0.0 0.0 5.2942776404249035 0.002616926192814264 84-85 6.435064408559666E-5 0.0 0.0 5.736130612931305 0.002788527910375855 86-87 8.580085878079554E-5 0.0 0.0 6.1887086927853066 0.002917229198547048 88-89 8.580085878079554E-5 0.0 0.0 6.663251792487191 0.0030459304867182417 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9225 0.0 34.565945 1 GGTATCA 5990 0.0 34.244473 1 TAAGGTG 1425 0.0 28.68127 5 ATCAACG 11595 0.0 27.3382 3 TATCAAC 11595 0.0 27.256226 2 GTAAGGT 1400 0.0 27.156683 4 GTACATG 10190 0.0 26.855528 1 TCAACGC 11915 0.0 26.68375 4 CAACGCA 11995 0.0 26.426546 5 AGGTAAG 1365 0.0 26.1122 2 AACGCAG 12390 0.0 25.88535 6 TACATGG 10280 0.0 25.84244 2 GGTAAGG 1510 0.0 24.548923 3 ACATGGG 10545 0.0 24.201513 3 AAGGTAA 1470 0.0 23.634453 1 ACGCAGA 14115 0.0 22.482878 7 CGCAGAG 14215 0.0 22.157612 8 GGACCGA 890 0.0 21.354605 6 GAGTACT 6760 0.0 21.00904 12-13 GTGTAAG 865 0.0 20.907759 1 >>END_MODULE