FastQCFastQC Report
Thu 26 May 2016
SRR938554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330979
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70670.30317733450194106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55090.23633846551170132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41720.1789805914167395No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36630.15714427285702703No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35880.1539267406527472No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33140.1421720229997782No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30170.12943059547083005No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29870.12814358258911815No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28160.12080760916336011No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25490.10935319451612392No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24340.10441964513622816No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG23940.10270362796061226No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA23320.1000438013384076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.036.341321
GTATCAA94400.035.4735071
TATCAAC118850.027.8550622
ATCAACG118550.027.7896633
TCAACGC120650.027.3053784
CAACGCA123100.026.8005525
GTACATG104250.026.0989741
AACGCAG126950.026.0017726
TACATGG104000.025.795262
ACATGGG105200.024.0859133
TAAGGTA6700.023.4143144
ACGCAGA143500.022.870577
TAAGGTG14050.022.669445
CGCAGAG145200.022.5700918
GGACCGA8750.022.2549516
GTGTAAG8500.021.8247431
GAGTACT72300.021.5469812-13
AGTACTT77250.021.21150412-13
GTAAGGT13350.021.0098233
AGAGTAC127050.020.76635410-11
Produced by FastQC (version 0.11.5)