##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2330979 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787655744646347 31.0 31.0 34.0 30.0 34.0 2 32.01107603286001 31.0 31.0 34.0 30.0 34.0 3 32.07615512623666 33.0 31.0 34.0 30.0 34.0 4 35.74723195704466 37.0 35.0 37.0 33.0 37.0 5 35.61472883282089 37.0 35.0 37.0 33.0 37.0 6 35.64804530628547 37.0 35.0 37.0 33.0 37.0 7 35.587402546312084 37.0 35.0 37.0 33.0 37.0 8 35.57314630462136 37.0 35.0 37.0 33.0 37.0 9 37.15147498111308 39.0 37.0 39.0 33.0 39.0 10-11 37.05984202345881 39.0 37.0 39.0 33.0 39.0 12-13 36.95096352219389 39.0 37.0 39.0 33.0 39.0 14-15 38.00536426969097 40.0 37.0 41.0 33.0 41.0 16-17 37.93734306486674 40.0 37.0 41.0 33.0 41.0 18-19 38.041396554838116 40.0 37.0 41.0 33.0 41.0 20-21 38.08235187875995 40.0 37.0 41.0 33.0 41.0 22-23 37.992521382646515 40.0 37.0 41.0 33.0 41.0 24-25 37.883170118649716 40.0 37.0 41.0 32.5 41.0 26-27 37.69248178555019 40.0 37.0 41.0 32.0 41.0 28-29 37.57657233291248 39.5 37.0 41.0 32.0 41.0 30-31 37.34289197800581 39.0 36.0 41.0 31.5 41.0 32-33 37.12931819634583 39.0 36.0 41.0 31.0 41.0 34-35 36.97716088390329 39.0 36.0 41.0 31.0 41.0 36-37 36.97647683655666 39.0 36.0 41.0 31.0 41.0 38-39 36.85131461930803 39.0 35.0 41.0 30.0 41.0 40-41 36.68138387347119 39.0 35.0 41.0 30.0 41.0 42-43 36.45835655319074 39.0 35.0 40.0 30.0 41.0 44-45 36.1915032267558 39.0 35.0 40.0 30.0 41.0 46-47 35.82243147621664 38.0 35.0 40.0 28.5 41.0 48-49 35.453359725677494 38.0 34.5 40.0 27.5 41.0 50-51 35.08496451490983 38.0 34.0 40.0 26.5 41.0 52-53 34.58663119659165 38.0 33.0 40.0 25.5 41.0 54-55 34.263646948342306 37.5 33.0 40.0 24.5 41.0 56-57 33.91812238548695 37.0 33.0 40.0 24.0 41.0 58-59 33.467338616092206 36.5 32.0 39.0 23.0 41.0 60-61 33.08613934316868 36.0 32.0 39.0 22.0 40.0 62-63 32.62522828390989 35.5 31.0 39.0 21.5 40.0 64-65 32.12009353151616 35.0 31.0 38.0 19.5 40.0 66-67 31.62706506579424 35.0 30.0 38.0 18.5 40.0 68-69 31.084549882259772 34.5 29.5 37.0 16.5 39.5 70-71 30.505278468832195 34.0 29.0 36.5 12.5 39.0 72-73 30.067844455055152 34.0 29.0 36.0 9.5 39.0 74-75 29.560567469719807 34.0 28.5 36.0 7.5 38.5 76-77 28.31208389264768 31.5 26.5 34.5 7.0 36.0 78-79 29.016753475685537 33.0 28.5 35.0 6.0 37.0 80-81 28.757417591492676 33.0 28.0 35.0 2.0 36.5 82-83 28.303836499599527 33.0 27.0 35.0 2.0 36.0 84-85 27.80679384069955 33.0 26.0 35.0 2.0 36.0 86-87 27.260679954645667 32.5 25.5 35.0 2.0 35.0 88-89 26.622721611820616 32.0 25.0 34.0 2.0 35.0 90-91 26.097747770357437 32.0 24.0 34.0 2.0 35.0 92-93 25.367509745905046 31.0 21.5 34.0 2.0 35.0 94-95 24.618916343733684 31.0 18.5 34.0 2.0 35.0 96-97 23.627860868759434 31.0 5.0 34.0 2.0 35.0 98-99 22.39094968251537 30.0 2.0 34.0 2.0 35.0 100-101 18.722125982258955 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4005519932539663 1101 2 0.4619479980088741 1101 3 0.4241182043736842 1101 4 0.2922280040218297 1101 5 0.16381685935298407 1101 6 0.22785181333007642 1101 7 0.29284194440139544 1101 8 0.29288445666719554 1101 9 0.22273882402328837 1101 10-11 0.35419862978172745 1101 12-13 0.5048531936685734 1101 14-15 0.5511801887443042 1101 16-17 0.5192277499517814 1101 18-19 0.5344059226213105 1101 20-21 0.4615016864740866 1101 22-23 0.5135859270096645 1101 24-25 0.4943751512836059 1101 26-27 0.5354672296732517 1101 28-29 0.2927023975966563 1101 30-31 0.46786411595096666 1101 32-33 0.38720455191283776 1101 34-35 0.3237067069123256 1101 36-37 0.48349739622969423 1101 38-39 0.5222579464264143 1101 40-41 0.6732718678857239 1101 42-43 0.6633461648207017 1101 44-45 0.5918052831399265 1101 46-47 0.7484274992980957 1101 48-49 0.8194587400604121 1101 50-51 0.8945817463719479 1101 52-53 0.7866051289641618 1101 54-55 0.7039870878747436 1101 56-57 0.7083998687135633 1101 58-59 0.8404165610426872 1101 60-61 0.9191653763524243 1101 62-63 0.8337979057132046 1101 64-65 0.854204326740124 1101 66-67 0.7127389353156914 1101 68-69 0.7316005829214447 1101 70-71 0.5858722540906172 1101 72-73 0.6118283519583905 1101 74-75 0.6192456230676768 1101 76-77 0.6429482608417096 1101 78-79 0.9388405734234162 1101 80-81 0.8182751031734803 1101 82-83 0.7410867944156614 1101 84-85 0.6259751921235868 1101 86-87 0.8804669109737411 1101 88-89 0.8715108267767029 1101 90-91 0.8664433532887479 1101 92-93 1.0658929254266667 1101 94-95 1.1290642485236155 1101 96-97 0.9437371674647359 1101 98-99 0.681994140762928 1101 100-101 0.5225111181994357 1102 1 0.31356311114683777 1102 2 0.34263005712637096 1102 3 0.4227349675765524 1102 4 0.3007478907610448 1102 5 0.2203173527630895 1102 6 0.2571217870372564 1102 7 0.3302469008368263 1102 8 0.35089712779934246 1102 9 0.341749570827254 1102 10-11 0.3805376918401109 1102 12-13 0.34170186211367337 1102 14-15 0.5077175032310279 1102 16-17 0.4624961414463655 1102 18-19 0.4882480455875893 1102 20-21 0.5013065366867551 1102 22-23 0.5337270942972694 1102 24-25 0.5381398502132555 1102 26-27 0.3990824607432586 1102 28-29 0.34377912147637346 1102 30-31 0.3189195031776393 1102 32-33 0.37925818366255015 1102 34-35 0.43787574117358474 1102 36-37 0.45834624081731334 1102 38-39 0.47844911593019646 1102 40-41 0.5146950610194594 1102 42-43 0.5045771937635308 1102 44-45 0.36179213385184994 1102 46-47 0.338575146250939 1102 48-49 0.37654531986208184 1102 50-51 0.5797661462852943 1102 52-53 0.3263362986483074 1102 54-55 0.45105881452612095 1102 56-57 0.4457265779627164 1102 58-59 0.4658927742242511 1102 60-61 0.49662471822796306 1102 62-63 0.39167635932008693 1102 64-65 0.4075509481521067 1102 66-67 0.36748915160833917 1102 68-69 0.4270667743998686 1102 70-71 0.41914703314976975 1102 72-73 0.05966340121017666 1102 74-75 -0.014429179860897534 1102 76-77 0.07881032014724454 1102 78-79 0.11719868343343265 1102 80-81 0.2635847714978965 1102 82-83 0.3045503855854328 1102 84-85 0.12407705507051148 1102 86-87 -0.017581495214734844 1102 88-89 0.04812880992957247 1102 90-91 0.0743243300699774 1102 92-93 0.3022890342505029 1102 94-95 0.48444472114966786 1102 96-97 0.19702290650250376 1102 98-99 0.16058275186385984 1102 100-101 -0.050759850194879164 1103 1 0.2538878574306942 1103 2 0.3073328377789366 1103 3 0.29864145423321276 1103 4 0.16211573495343146 1103 5 0.20182422772994357 1103 6 0.1320714034919419 1103 7 0.22501898922411812 1103 8 0.15286152058445168 1103 9 0.297690342397253 1103 10-11 0.29836013633292424 1103 12-13 0.34343919058250094 1103 14-15 0.5342558653644076 1103 16-17 0.39055483517114453 1103 18-19 0.3674404478787423 1103 20-21 0.393989677861164 1103 22-23 0.3866743509294537 1103 24-25 0.3831285461295124 1103 26-27 0.3251935778285002 1103 28-29 0.40355579119726315 1103 30-31 0.5089664215216345 1103 32-33 0.49882134951794654 1103 34-35 0.6361113993374943 1103 36-37 0.5443863184941833 1103 38-39 0.4977442060977353 1103 40-41 0.5662907778897122 1103 42-43 0.5990053205932355 1103 44-45 0.6813985544675987 1103 46-47 0.6659306210280178 1103 48-49 0.8046067019453105 1103 50-51 0.7824595662591705 1103 52-53 0.8932881970557958 1103 54-55 0.7626814594777755 1103 56-57 0.8246414304200442 1103 58-59 0.8450041549540188 1103 60-61 0.7516880096018923 1103 62-63 0.6122109310534611 1103 64-65 0.5099229887793228 1103 66-67 0.6636867374253796 1103 68-69 0.5301224923899532 1103 70-71 0.7159588625202176 1103 72-73 0.5157220897436439 1103 74-75 0.5799534302792964 1103 76-77 0.37738395131855285 1103 78-79 0.4798198588727409 1103 80-81 0.3678780613923003 1103 82-83 0.6560513053748309 1103 84-85 0.5163163389956473 1103 86-87 0.6329089169866293 1103 88-89 0.6667643067178197 1103 90-91 0.6016935803665326 1103 92-93 0.6721076868600804 1103 94-95 0.568265327176114 1103 96-97 0.5838689725812394 1103 98-99 0.5212125026103891 1103 100-101 0.23718660734592945 1104 1 0.2200680591065698 1104 2 0.20688808630288946 1104 3 0.20584386587835724 1104 4 0.14047909057447328 1104 5 0.06816927800735328 1104 6 0.04355127890348598 1104 7 0.18604068249317862 1104 8 0.2095906029850454 1104 9 0.16474320118273056 1104 10-11 0.25731538266266796 1104 12-13 0.21951722717832212 1104 14-15 0.44411238196268954 1104 16-17 0.39279867661778667 1104 18-19 0.3273997205771835 1104 20-21 0.34469258479531817 1104 22-23 0.40980190545460005 1104 24-25 0.3404020394440863 1104 26-27 0.36488995629087384 1104 28-29 0.4779951272304146 1104 30-31 0.39468843751598115 1104 32-33 0.373476071680102 1104 34-35 0.34779179689937934 1104 36-37 0.42476898100790095 1104 38-39 0.3984104485511679 1104 40-41 0.4599444960254928 1104 42-43 0.45653160771646384 1104 44-45 0.3604745493897781 1104 46-47 0.49922012921789616 1104 48-49 0.5982150495557264 1104 50-51 0.6976997628459074 1104 52-53 0.7766603772794127 1104 54-55 0.8196204071010058 1104 56-57 0.6602700687769669 1104 58-59 0.5765930824224128 1104 60-61 0.6794050121885817 1104 62-63 0.6101044929334449 1104 64-65 0.5878200578504575 1104 66-67 0.6646500648048601 1104 68-69 0.6293813107107304 1104 70-71 0.37601701606723736 1104 72-73 0.20211655921108118 1104 74-75 0.15340475411124288 1104 76-77 0.36625481271797256 1104 78-79 0.33548177838570936 1104 80-81 0.4179148143490856 1104 82-83 0.4708681616928878 1104 84-85 0.5195650030955647 1104 86-87 0.4525678028323412 1104 88-89 0.12506120265899412 1104 90-91 0.13542888405000397 1104 92-93 0.1613450161145522 1104 94-95 0.45874912490851116 1104 96-97 0.49525559422184884 1104 98-99 0.38836003078382575 1104 100-101 -0.009362649225099773 1105 1 0.24814957638060164 1105 2 0.22907038496405363 1105 3 0.16733810933372695 1105 4 0.1762276660731672 1105 5 0.12929709683217538 1105 6 0.1902209775682664 1105 7 0.1527816792918415 1105 8 0.19570349975006707 1105 9 0.22646418561124904 1105 10-11 0.1961797078992049 1105 12-13 0.3027524175936023 1105 14-15 0.3610256096570268 1105 16-17 0.29267811752499995 1105 18-19 0.294166397003238 1105 20-21 0.2412857091764593 1105 22-23 0.3199268419101955 1105 24-25 0.40397685644717285 1105 26-27 0.35866450504322245 1105 28-29 0.34172035589313765 1105 30-31 0.364279788482321 1105 32-33 0.3562517150265663 1105 34-35 0.3520591765586474 1105 36-37 0.305904743622726 1105 38-39 0.4014932709246821 1105 40-41 0.2262839614398544 1105 42-43 0.2592742257105485 1105 44-45 0.16460538776710365 1105 46-47 0.08954965458911346 1105 48-49 0.07755469117963543 1105 50-51 0.07656439894108047 1105 52-53 0.14861254762184473 1105 54-55 0.18504268745530084 1105 56-57 0.15394662947743853 1105 58-59 0.2761547041517005 1105 60-61 0.10051356453506344 1105 62-63 0.2290371530100188 1105 64-65 0.26617824776786136 1105 66-67 0.18047285442671068 1105 68-69 0.1353933000557106 1105 70-71 0.20701009625238243 1105 72-73 0.18224399098309974 1105 74-75 0.20101199569858608 1105 76-77 0.26896906486048877 1105 78-79 0.2869261645066139 1105 80-81 0.3657593221779045 1105 82-83 0.3516123099900348 1105 84-85 0.3181320629927882 1105 86-87 0.3518382817861081 1105 88-89 0.31161203843794993 1105 90-91 0.44912251156599226 1105 92-93 0.3982394943019898 1105 94-95 0.5699410667765896 1105 96-97 0.37795283023232784 1105 98-99 0.2377016271004173 1105 100-101 0.09317787685923307 1106 1 0.13713443954179638 1106 2 0.18086597962843598 1106 3 0.1795845115608472 1106 4 0.08503692239499827 1106 5 -0.013230523699647279 1106 6 0.06907299950056256 1106 7 0.17439270316790356 1106 8 0.21369082437669107 1106 9 0.06578910454035736 1106 10-11 0.08572729398349566 1106 12-13 0.13675215158821885 1106 14-15 0.288117826136137 1106 16-17 0.024776562159168236 1106 18-19 0.3043008196158894 1106 20-21 0.2567214295059088 1106 22-23 0.2648990153823334 1106 24-25 0.17496885181554944 1106 26-27 0.15539876449037138 1106 28-29 0.2991042278097069 1106 30-31 0.4054343850571769 1106 32-33 0.2833265362844273 1106 34-35 0.13820465687233252 1106 36-37 -0.23775310019881601 1106 38-39 0.0905525257067481 1106 40-41 0.3823200116626069 1106 42-43 0.4498819048767544 1106 44-45 0.2988900871135911 1106 46-47 0.14596185774465908 1106 48-49 0.14968018912824732 1106 50-51 0.20091309772027444 1106 52-53 0.08750513287964878 1106 54-55 -0.28383293371640406 1106 56-57 0.10871422682451026 1106 58-59 -0.06849789663363737 1106 60-61 -0.2916111776785755 1106 62-63 -0.32204428875252944 1106 64-65 -0.8001499805812884 1106 66-67 -0.4213107466903807 1106 68-69 -0.9785718665547201 1106 70-71 -0.6501228683244555 1106 72-73 -0.26322548606328056 1106 74-75 -0.18687596975466647 1106 76-77 -0.207194654633998 1106 78-79 -0.2321769512022378 1106 80-81 -0.9752022457024907 1106 82-83 -0.6769395736294435 1106 84-85 -0.2821309960326097 1106 86-87 0.048002444068043104 1106 88-89 0.017690145145397906 1106 90-91 -0.10246763637994505 1106 92-93 -0.3481927791102706 1106 94-95 -0.23133725726292198 1106 96-97 -0.209652250637145 1106 98-99 -0.4131747008665876 1106 100-101 -0.6868111100514014 1107 1 0.19040991743784375 1107 2 0.2174571683120874 1107 3 0.15949432021979248 1107 4 0.10480521146649835 1107 5 0.08073053101787053 1107 6 0.168581731629601 1107 7 0.011288541203121838 1107 8 0.2209032942248541 1107 9 0.13213081407297267 1107 10-11 0.17150878594018337 1107 12-13 0.11932262763829016 1107 14-15 0.20448699683500848 1107 16-17 0.2614916522330972 1107 18-19 0.18700992237283742 1107 20-21 0.18972759881020096 1107 22-23 0.2561209818366521 1107 24-25 0.2626899924780375 1107 26-27 0.3514448294552679 1107 28-29 0.41567320947660846 1107 30-31 0.3365074284979954 1107 32-33 0.4833265852057522 1107 34-35 0.3777396532260653 1107 36-37 0.20814326553434626 1107 38-39 0.2581913676674006 1107 40-41 0.2619023084075778 1107 42-43 0.3356811226574976 1107 44-45 0.24412568891754916 1107 46-47 0.2480632566334222 1107 48-49 0.3658032366784667 1107 50-51 0.37258794837979536 1107 52-53 0.445612846532093 1107 54-55 0.3084548488694381 1107 56-57 0.37504106427658 1107 58-59 0.31327183529059255 1107 60-61 0.31546845448529837 1107 62-63 0.4094006708515039 1107 64-65 0.3980002717874882 1107 66-67 0.36509445945100794 1107 68-69 0.38001543908088564 1107 70-71 0.33111654021029224 1107 72-73 0.30331949154379956 1107 74-75 0.24991564956703627 1107 76-77 0.20755456816933915 1107 78-79 0.28632313424699873 1107 80-81 0.4793035381018136 1107 82-83 0.4071835671974178 1107 84-85 0.43402221966343646 1107 86-87 0.528684557260231 1107 88-89 0.4464014304453876 1107 90-91 0.5106298885927565 1107 92-93 0.3758397855278304 1107 94-95 0.26880901747658825 1107 96-97 0.24858851269195625 1107 98-99 0.06720467090542215 1107 100-101 -0.37833325771028825 1108 1 0.14797965621782083 1108 2 0.19776863778072595 1108 3 0.141119536446638 1108 4 0.04456659727173928 1108 5 0.049515792379260404 1108 6 -0.061708211540967284 1108 7 0.06745001028621544 1108 8 0.06047807594176646 1108 9 0.13337066458407776 1108 10-11 0.09832499504985748 1108 12-13 0.1499108546167207 1108 14-15 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-0.5002374628087551 2316 70-71 -0.6100660099205513 2316 72-73 -0.5583400441538551 2316 74-75 -0.428484003115976 2316 76-77 -0.3827919996002862 2316 78-79 -0.3207925827833087 2316 80-81 -0.5628909273029556 2316 82-83 -0.5027146994285765 2316 84-85 -0.2731969833805401 2316 86-87 -0.0771407620947997 2316 88-89 0.07245201082331576 2316 90-91 0.11810516433247642 2316 92-93 0.17505885745830696 2316 94-95 -0.014490467167078691 2316 96-97 0.17873645688577255 2316 98-99 0.4013062892206385 2316 100-101 0.4998940743707756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 19.0 4 45.0 5 70.0 6 184.0 7 435.0 8 1052.0 9 1901.0 10 2987.0 11 4555.0 12 6423.0 13 8683.0 14 11286.0 15 15091.0 16 18906.0 17 23441.0 18 22812.0 19 19455.0 20 19578.0 21 20675.0 22 22425.0 23 25523.0 24 29709.0 25 34869.0 26 41521.0 27 50572.0 28 60227.0 29 72638.0 30 86365.0 31 103221.0 32 124649.0 33 153920.0 34 197203.0 35 258858.0 36 339315.0 37 373068.0 38 172746.0 39 6548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.240993854902946 18.71907931654028 14.631242696154894 23.408684132401874 2 16.381054574328818 20.299590102367105 38.360323625984506 24.95903169731957 3 19.86529126473913 23.516670205988532 29.217724065887158 27.40031446338518 4 13.637402996766596 16.420053548315966 35.293453952180606 34.649089502736835 5 14.231856091395514 37.24441971138756 33.33024170122209 15.193482495994834 6 33.73144931807622 35.31370295485288 16.422155669356094 14.532692057714804 7 30.25132358549777 32.10058949480025 19.316690540755623 18.33139637894636 8 27.69325678180713 34.26062611460678 18.841696986545138 19.20442011704095 9 27.02576900092193 16.402507272695292 19.081596187696242 37.49012753868654 10-11 25.87846136751983 26.143543120723095 26.33691680620031 21.64107870555676 12-13 26.80004839168435 23.260098010320984 26.902837820503745 23.037015777490915 14-15 23.062670234266374 24.79453911854204 25.1163781398288 27.026412507362785 16-17 22.98819508884464 28.11170757008107 25.978784021649272 22.921313319425014 18-19 23.11277793579436 26.27683475483906 28.29948274952284 22.31090455984374 20-21 23.42091026989089 25.46043958353979 28.489188448287177 22.62946169828214 22-23 23.297700236681667 25.37215049985435 28.426339319230248 22.903809944233732 24-25 23.256862520922024 25.662206375739956 28.356570733338582 22.72436036999944 26-27 22.500078835643663 26.128985409935694 28.342196890893828 23.02873886352681 28-29 22.689157645778877 26.548694775886013 28.215397907917662 22.54674967041745 30-31 22.69490630331719 25.90467352987736 28.06657631836237 23.333843848443077 32-33 22.441772319699147 26.004073824774913 28.03268497914396 23.521468876381984 34-35 23.436660012193432 26.04741451619062 27.76908248500598 22.746842986609963 36-37 23.524815109874435 25.463700016173462 27.859474495480224 23.15201037847188 38-39 22.77793901780583 25.931536554544426 27.979935591222848 23.31058883642689 40-41 23.257867282688043 25.746657419767672 28.017052849116737 22.97842244842755 42-43 22.949787245361 25.793065251977552 27.70061753221779 23.556529970443655 44-45 22.933501960657296 25.993568795250578 27.975268755160123 23.09766048893201 46-47 23.450275613808618 26.432434612238033 27.121050854598007 22.996238919355346 48-49 23.1988147469368 26.351417151334267 27.118605530122753 23.33116257160618 50-51 23.330433264306542 26.481984608183947 27.214702491957244 22.972879635552275 52-53 23.787086884952632 26.165079136277075 27.01744631762019 23.0303876611501 54-55 22.951107667636645 26.39983028590133 27.304321488953782 23.34474055750824 56-57 23.114901507049186 26.67081084814578 27.05524159591313 23.159046048891906 58-59 23.168226740781446 25.964648330165137 27.051037353832875 23.81608757522054 60-61 22.664146695444277 26.467891817129196 27.51712906894485 23.350832418481676 62-63 22.84046746023881 26.450366991723218 27.549561793563992 23.159603754473977 64-65 22.384929250756873 26.4670123583267 27.33844878053384 23.80960961038259 66-67 22.43253690082017 26.515565569473416 27.23526348168022 23.816634048026195 68-69 22.990355904863232 26.000703572776885 27.50083656519202 23.50810395716786 70-71 22.800262979801392 26.265054038488145 27.018300262443546 23.916382719266917 72-73 22.851128216942325 26.270120837639467 27.06060414958693 23.81814679583128 74-75 22.69329753721505 26.789902440133524 26.82825542400854 23.68854459864289 76-77 22.7703681586149 26.31297836660047 27.17087970333495 23.74577377144968 78-79 22.942334529826308 26.531920707994367 27.055434647845388 23.470310114333934 80-81 22.949530535566122 26.703508520545665 26.861748451492396 23.485212492395817 82-83 22.84954943553421 26.65108656715137 26.884144549574962 23.615219447739456 84-85 22.716963469227483 26.669971583577894 26.90812295234763 23.70494199484699 86-87 23.014093969521127 26.678946478781395 26.64544048159401 23.66151907010347 88-89 22.680444878216196 26.853805810873133 26.420188976715753 24.045560334194917 90-91 22.595372950042517 26.763008483411145 26.71736091864866 23.924257647897683 92-93 22.775585902182215 26.35636915575216 26.881454029708408 23.986590912357222 94-95 22.44171058781588 26.171670971371867 27.101881979505155 24.284736461307098 96-97 22.715284007277628 25.875179484671463 27.076241356099732 24.333295151951177 98-99 22.35992309667378 26.297411151582907 26.625856909448114 24.716808842295197 100-101 22.40526405428792 25.950105084601795 27.092178865618266 24.55245199549202 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 514.0 1 343.5 2 255.0 3 364.5 4 326.5 5 308.0 6 570.5 7 1280.0 8 2011.5 9 2241.0 10 1941.5 11 1583.5 12 1652.5 13 1817.0 14 1936.5 15 2108.0 16 2270.0 17 2406.5 18 2568.5 19 2825.5 20 3136.5 21 3562.0 22 4153.5 23 4954.0 24 5942.5 25 7327.0 26 9187.5 27 11676.5 28 14243.5 29 16929.5 30 20321.5 31 25233.0 32 33247.5 33 36203.5 34 35107.5 35 37887.5 36 42681.5 37 47716.5 38 51986.0 39 54732.5 40 58108.5 41 65410.0 42 70712.5 43 74208.5 44 78407.0 45 83431.0 46 86835.0 47 89966.5 48 95302.0 49 94578.0 50 91240.5 51 89238.0 52 87663.5 53 89401.0 54 91817.5 55 86722.0 56 78532.0 57 72897.5 58 67871.5 59 59712.0 60 48132.0 61 40073.0 62 34185.0 63 25674.5 64 18218.0 65 14006.5 66 10639.5 67 8426.5 68 6503.0 69 4657.0 70 3425.0 71 2492.5 72 1879.5 73 1581.5 74 1221.0 75 925.0 76 646.0 77 383.5 78 235.5 79 143.0 80 83.5 81 40.0 82 22.5 83 16.0 84 7.5 85 4.5 86 3.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.435064408559665E-4 2 0.048434584781759084 3 0.0010296103053695465 4 0.0 5 0.09060570687252009 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.0725107347599443E-4 26-27 0.007743527504966797 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009266492748325918 36-37 0.0 38-39 0.003667986712879009 40-41 0.00461179615946776 42-43 3.2175322042798325E-4 44-45 2.1450214695198885E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.290042939039777E-5 68-69 8.151081584175577E-4 70-71 6.435064408559666E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0010725107347599443 82-83 6.006060114655687E-4 84-85 0.004654696588858157 86-87 0.0022737227576910815 88-89 1.7160171756159108E-4 90-91 0.003303333063060628 92-93 9.652596612839498E-4 94-95 1.0725107347599443E-4 96-97 0.0 98-99 2.1450214695198885E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2330979.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.00398924633364 #Duplication Level Percentage of deduplicated Percentage of total 1 73.74108483499053 34.661251585968714 2 13.688903617231457 12.8686615683689 3 4.979436524762607 7.021601425882275 4 2.3345840297245255 4.3893905051133535 5 1.3035952861798876 3.0637089406585325 6 0.822132127389766 2.3186093804936374 7 0.5516887863462412 1.8152101646519103 8 0.38283450965073357 1.4395799339798796 9 0.29605882109480375 1.2524351078720133 >10 1.681698594172093 14.687583661547501 >50 0.13558378398852405 4.386641734003143 >100 0.07351316012128847 6.53690460666112 >500 0.004855195777582584 1.626172394167744 >1k 0.0038475136350653743 3.390736941988336 >5k 1.8321493500311306E-4 0.5415120486430463 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7067 0.30317733450194106 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5509 0.23633846551170132 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4172 0.1789805914167395 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3663 0.15714427285702703 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3588 0.1539267406527472 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3314 0.1421720229997782 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3017 0.12943059547083005 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2987 0.12814358258911815 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2816 0.12080760916336011 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2549 0.10935319451612392 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2434 0.10441964513622816 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2394 0.10270362796061226 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2332 0.1000438013384076 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.290042939039777E-5 2 0.0 0.0 0.0 0.0 4.290042939039777E-5 3 0.0 0.0 0.0 4.290042939039777E-5 4.290042939039777E-5 4 0.0 0.0 0.0 4.290042939039777E-5 4.290042939039777E-5 5 0.0 0.0 0.0 4.290042939039777E-5 4.290042939039777E-5 6 0.0 0.0 0.0 4.290042939039777E-5 4.290042939039777E-5 7 0.0 0.0 0.0 4.290042939039777E-5 4.290042939039777E-5 8 0.0 0.0 0.0 4.290042939039777E-5 4.290042939039777E-5 9 0.0 0.0 0.0 4.290042939039777E-5 4.290042939039777E-5 10-11 0.0 0.0 0.0 4.290042939039777E-5 4.290042939039777E-5 12-13 0.0 0.0 0.0 4.290042939039777E-5 2.3595236164718772E-4 14-15 0.0 0.0 0.0 4.290042939039777E-5 4.075540792087788E-4 16-17 0.0 0.0 0.0 1.501515028663922E-4 5.791557967703699E-4 18-19 0.0 0.0 0.0 2.1450214695198887E-4 6.006060114655688E-4 20-21 0.0 0.0 0.0 3.003030057327844E-4 7.078570849415632E-4 22-23 0.0 0.0 0.0 4.7190472329437544E-4 7.722077290271598E-4 24-25 0.0 0.0 0.0 9.438094465887509E-4 8.151081584175577E-4 26-27 0.0 0.0 0.0 0.0015229652433591209 8.580085878079554E-4 28-29 0.0 0.0 0.0 0.00364653649818381 9.009090171983532E-4 30-31 0.0 0.0 0.0 0.011947769585225778 9.438094465887509E-4 32-33 0.0 0.0 0.0 0.030845408731695993 0.0010296103053695465 34-35 0.0 0.0 0.0 0.06010350157594727 0.001115411164150342 36-37 0.0 0.0 0.0 0.10665046746452886 0.001115411164150342 38-39 0.0 0.0 0.0 0.18224102405040973 0.001115411164150342 40-41 0.0 0.0 0.0 0.2646956493387542 0.001115411164150342 42-43 0.0 0.0 0.0 0.34890919223210504 0.001115411164150342 44-45 0.0 0.0 0.0 0.453843642521018 0.0011368613788455407 46-47 0.0 0.0 0.0 0.5757666628485285 0.0012226622376263364 48-49 0.0 0.0 0.0 0.709680353190655 0.0013299133111023308 50-51 0.0 0.0 0.0 0.8595959036954002 0.0013728137404927286 52-53 0.0 0.0 0.0 1.0036984460177463 0.0015444154580543197 54-55 0.0 0.0 0.0 1.1639100995761866 0.0015873158874447175 56-57 0.0 0.0 0.0 1.3471378335025754 0.0016302163168351153 58-59 0.0 0.0 0.0 1.5400825146858894 0.0016945669609207117 60-61 0.0 0.0 0.0 1.7555713715138574 0.0017160171756159107 62-63 0.0 0.0 0.0 1.974127609043239 0.0019305193225678996 64-65 0.0 0.0 0.0 2.202057590394422 0.0019305193225678996 66-67 0.0 0.0 0.0 2.4690055122761727 0.0019305193225678996 68-69 0.0 0.0 0.0 2.758197306796844 0.0019305193225678996 70-71 0.0 0.0 0.0 3.068968017300885 0.0019305193225678996 72-73 0.0 0.0 0.0 3.396405544623096 0.0019734197519582974 74-75 0.0 0.0 0.0 3.721848201978654 0.0021235712548246894 76-77 0.0 0.0 0.0 4.074210878776686 0.0023166231870814795 78-79 0.0 0.0 0.0 4.4794697850130785 0.0023595236164718775 80-81 0.0 0.0 0.0 4.904205486192711 0.002423874260557474 82-83 0.0 0.0 0.0 5.341682614901293 0.0026598266222046616 84-85 0.0 0.0 0.0 5.792180023929859 0.0026598266222046616 86-87 0.0 0.0 0.0 6.255375960057984 0.002767077695680656 88-89 0.0 0.0 0.0 6.7446768074701655 0.0028743287691566505 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5890 0.0 36.34132 1 GTATCAA 9440 0.0 35.473507 1 TATCAAC 11885 0.0 27.855062 2 ATCAACG 11855 0.0 27.789663 3 TCAACGC 12065 0.0 27.305378 4 CAACGCA 12310 0.0 26.800552 5 GTACATG 10425 0.0 26.098974 1 AACGCAG 12695 0.0 26.001772 6 TACATGG 10400 0.0 25.79526 2 ACATGGG 10520 0.0 24.085913 3 TAAGGTA 670 0.0 23.414314 4 ACGCAGA 14350 0.0 22.87057 7 TAAGGTG 1405 0.0 22.66944 5 CGCAGAG 14520 0.0 22.570091 8 GGACCGA 875 0.0 22.254951 6 GTGTAAG 850 0.0 21.824743 1 GAGTACT 7230 0.0 21.54698 12-13 AGTACTT 7725 0.0 21.211504 12-13 GTAAGGT 1335 0.0 21.009823 3 AGAGTAC 12705 0.0 20.766354 10-11 >>END_MODULE