FastQCFastQC Report
Thu 26 May 2016
SRR938553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335603
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70070.30000817775966204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52370.22422475052481095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39270.16813645127189852No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36730.15726131538621932No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35290.15109588401796026No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33760.14454511318918498No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30510.13063007711498914No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29160.12484998520724626No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27300.11688630302324496No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25250.10810912642259837No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24520.1049835952428559No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA23950.10254311199292003No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG23770.10177243307188763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93700.034.8480261
GGTATCA59900.034.3782771
GTGTAAG9500.031.1096631
TATCAAC118150.027.433572
ATCAACG118100.027.4049423
TCAACGC119750.027.0670244
CAACGCA119800.026.9367145
TAAGGTG13450.026.8548665
AACGCAG124200.026.0544996
GTACATG102450.026.0092051
GTAAGGT13750.025.5776544
TACATGG102900.025.0793422
TAAGGTA7750.023.3030954
ACATGGG107350.023.110013
ACGCAGA138400.023.0344877
CGCAGAG140300.022.688688
TGTAAGG9950.021.971842
GAGTACT68050.021.77931212-13
GGTAAGG16100.021.5490763
AGGTAAG14400.021.4527322
Produced by FastQC (version 0.11.5)