FastQCFastQC Report
Thu 26 May 2016
SRR938553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335603
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70460.30167798208856555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56180.24053745435332974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40660.17408780516209305No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36600.15670471394325147No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36460.15610529700467074No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33220.14223307642608782No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA29600.12673386701421432No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28910.12377959781692352No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28590.12240950195731039No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25340.10849446588311455No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24460.10472670226917846No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG24370.10434136280866227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67450.032.5079421
GTATCAA98150.031.5472261
TAAGGTG14750.028.3620575
GTAAGGT13350.027.7754064
AGGTAAG12750.027.2322272
ATCAACG123350.024.8966263
TATCAAC124850.024.724452
GGTAAGG14650.024.6617013
GTACATG97500.024.3913521
TCAACGC127250.024.1335834
CAACGCA128450.023.9081255
AAGGTAA12950.023.8733881
TACATGG98600.023.733332
AACGCAG132200.023.2444866
ACATGGG101650.022.12083
GAGTACT72100.021.17838512-13
ACGCAGA146350.020.8672587
CGCAGAG147250.020.6752058
AGTACTT73700.020.42861412-13
GTACTTT73500.020.35496314-15
Produced by FastQC (version 0.11.5)