##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938553_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335603 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7982452497278 31.0 31.0 34.0 30.0 34.0 2 32.02460863425848 31.0 31.0 34.0 30.0 34.0 3 32.09330053095496 33.0 31.0 34.0 30.0 34.0 4 35.768684147091776 37.0 35.0 37.0 35.0 37.0 5 35.63714852224458 37.0 35.0 37.0 33.0 37.0 6 35.680418718420896 37.0 35.0 37.0 33.0 37.0 7 35.61344543571831 37.0 35.0 37.0 33.0 37.0 8 35.59506902500125 37.0 35.0 37.0 33.0 37.0 9 37.158417333767765 39.0 37.0 39.0 33.0 39.0 10-11 37.09596472516947 39.0 37.0 39.0 33.0 39.0 12-13 36.986663615349016 39.0 37.0 39.0 33.0 39.0 14-15 38.035204613112754 40.0 37.0 41.0 33.0 41.0 16-17 37.95701730987672 40.0 37.0 41.0 33.0 41.0 18-19 37.95637935899209 40.0 37.0 41.0 33.0 41.0 20-21 37.944248230542605 40.0 37.0 41.0 32.5 41.0 22-23 37.97590943323844 40.0 37.0 41.0 33.0 41.0 24-25 37.89183521343311 40.0 37.0 41.0 32.5 41.0 26-27 37.71606069182134 40.0 37.0 41.0 32.0 41.0 28-29 37.58312542842255 39.5 37.0 41.0 32.0 41.0 30-31 37.34349309364648 39.0 36.0 41.0 31.5 41.0 32-33 37.13002445192954 39.0 36.0 41.0 31.0 41.0 34-35 36.959668231287594 39.0 36.0 40.5 31.0 41.0 36-37 36.96744309713594 39.0 36.0 41.0 31.0 41.0 38-39 36.819074560188525 39.0 35.0 41.0 30.0 41.0 40-41 36.6419402184361 39.0 35.0 40.5 30.0 41.0 42-43 36.40997785154412 39.0 35.0 40.0 30.0 41.0 44-45 36.12726156799765 38.5 35.0 40.0 30.0 41.0 46-47 35.7567940698826 38.0 35.0 40.0 28.5 41.0 48-49 35.34983257000441 38.0 34.0 40.0 27.0 41.0 50-51 34.9667856652008 38.0 34.0 40.0 26.0 41.0 52-53 34.438773841273544 38.0 33.0 40.0 24.5 41.0 54-55 34.10588207841829 37.0 33.0 40.0 24.0 41.0 56-57 33.73491149822979 37.0 33.0 40.0 23.0 41.0 58-59 33.26527838849324 36.0 32.0 39.0 22.5 41.0 60-61 32.838213514882455 36.0 31.0 39.0 21.5 40.0 62-63 32.35250661178291 35.0 31.0 39.0 19.5 40.0 64-65 31.845797851775323 35.0 30.5 38.0 18.0 40.0 66-67 31.339430973500207 35.0 30.0 38.0 16.5 40.0 68-69 30.7682925993844 34.0 29.0 37.0 12.5 39.5 70-71 30.199862519443585 34.0 29.0 36.5 8.0 39.0 72-73 29.619717049515693 34.0 28.0 36.0 7.0 39.0 74-75 29.004066829850792 33.5 27.5 35.5 4.5 38.0 76-77 27.744476051794763 31.5 26.0 34.5 2.0 36.0 78-79 28.379516553112836 33.0 27.0 35.0 2.0 37.0 80-81 28.094262381063906 33.0 26.0 35.0 2.0 36.0 82-83 27.612754821774075 33.0 26.0 35.0 2.0 36.0 84-85 27.04517698427344 32.0 25.5 35.0 2.0 35.5 86-87 26.416537185472016 32.0 24.5 34.5 2.0 35.0 88-89 25.66437789298952 31.5 22.0 34.0 2.0 35.0 90-91 25.129352034570942 31.0 20.0 34.0 2.0 35.0 92-93 24.361386545573026 31.0 17.5 34.0 2.0 35.0 94-95 23.576530343555817 31.0 4.5 34.0 2.0 35.0 96-97 22.59379847516894 30.0 2.0 34.0 2.0 35.0 98-99 21.41797600020209 29.5 2.0 34.0 2.0 35.0 100-101 18.273977212736924 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32247996825962844 1101 2 0.4100379235670957 1101 3 0.3524476325038961 1101 4 0.30935233175603827 1101 5 0.2610555828690124 1101 6 0.2616846797115926 1101 7 0.2976396658919427 1101 8 0.2138147401686652 1101 9 0.3360048934252049 1101 10-11 0.397617923541695 1101 12-13 0.3984100782584221 1101 14-15 0.469812912589461 1101 16-17 0.49872055086729716 1101 18-19 0.6114674700184892 1101 20-21 0.644024435438574 1101 22-23 0.5435711500636842 1101 24-25 0.6010182850448516 1101 26-27 0.6007362248433097 1101 28-29 0.5344176404774359 1101 30-31 0.663715565517272 1101 32-33 0.5984922555195666 1101 34-35 0.5121084478974112 1101 36-37 0.6352991851930341 1101 38-39 0.7161723638939321 1101 40-41 0.8285246292381672 1101 42-43 0.8008723269257132 1101 44-45 0.8275566904901339 1101 46-47 0.9477941106848391 1101 48-49 0.9666868768252286 1101 50-51 1.069811512880726 1101 52-53 1.112666273980345 1101 54-55 1.1496803511357712 1101 56-57 1.393396705195201 1101 58-59 1.4497002602553266 1101 60-61 1.5289981651330251 1101 62-63 1.7583862806590602 1101 64-65 1.6699054614896767 1101 66-67 1.6449960581575276 1101 68-69 1.679755205964252 1101 70-71 1.751625721787736 1101 72-73 1.878853349635925 1101 74-75 1.9174865987110223 1101 76-77 1.7921639954903554 1101 78-79 1.9929160383799207 1101 80-81 2.4403874567313117 1101 82-83 2.5990169173648603 1101 84-85 2.6537054263684574 1101 86-87 2.746687251137839 1101 88-89 2.934990666025616 1101 90-91 3.325135392508475 1101 92-93 3.4206502392605067 1101 94-95 3.518812699615591 1101 96-97 3.9302148547299005 1101 98-99 4.146600032939801 1101 100-101 4.635177077900288 1102 1 0.36765748270848064 1102 2 0.3408245379814474 1102 3 0.3672048414239981 1102 4 0.21346390672919568 1102 5 0.1937452591604938 1102 6 0.19221662223404223 1102 7 0.2898332165297859 1102 8 0.2918712874070053 1102 9 0.2889311005971038 1102 10-11 0.3317783590810208 1102 12-13 0.3861604377838077 1102 14-15 0.5175103026871 1102 16-17 0.4631388756146535 1102 18-19 0.5183654152000727 1102 20-21 0.6683190485659978 1102 22-23 0.601377017983161 1102 24-25 0.5588228931718717 1102 26-27 0.47289878458654044 1102 28-29 0.4540126462724814 1102 30-31 0.4477315125517194 1102 32-33 0.2674980660700328 1102 34-35 0.5804161396087579 1102 36-37 0.5529225188752207 1102 38-39 0.6638362241306268 1102 40-41 0.49162802137473705 1102 42-43 0.3857019060775073 1102 44-45 0.3913766138172221 1102 46-47 0.42841278507307123 1102 48-49 0.6262842665352437 1102 50-51 0.7372879353802375 1102 52-53 0.9197663673988004 1102 54-55 1.0069612732263309 1102 56-57 1.0347686773324654 1102 58-59 1.0596201568360542 1102 60-61 1.0857361166717183 1102 62-63 1.2363655929286708 1102 64-65 1.171271367241694 1102 66-67 1.265560813428305 1102 68-69 1.2796538411244747 1102 70-71 1.3579453253213565 1102 72-73 1.4334231014374268 1102 74-75 1.5952318803037073 1102 76-77 1.6351176855193899 1102 78-79 1.9401189114819921 1102 80-81 2.115029966392953 1102 82-83 1.9428223240064888 1102 84-85 2.2351969431198206 1102 86-87 2.345180151722772 1102 88-89 2.556104496996351 1102 90-91 2.5888302087532082 1102 92-93 2.8905163911565914 1102 94-95 3.05211240618085 1102 96-97 3.5389169196266543 1102 98-99 3.8133281387158533 1102 100-101 4.303489905688597 1103 1 0.2900332450392753 1103 2 0.2654075968435734 1103 3 0.2989543861135786 1103 4 0.1493761545510992 1103 5 0.057378702433481976 1103 6 0.2006586197117457 1103 7 0.18784330497659596 1103 8 0.20069960429474065 1103 9 0.2618362097109852 1103 10-11 0.308090218089184 1103 12-13 0.38067507631136266 1103 14-15 0.5212497249162311 1103 16-17 0.5046287185736631 1103 18-19 0.41974455508572106 1103 20-21 0.38792747971690034 1103 22-23 0.47957869394878117 1103 24-25 0.3693200328133699 1103 26-27 0.33259902234191685 1103 28-29 0.34978983347262016 1103 30-31 0.46344335954172067 1103 32-33 0.49085139942604883 1103 34-35 0.5327670870966017 1103 36-37 0.5978920520984303 1103 38-39 0.6423073826391175 1103 40-41 0.6491378371455028 1103 42-43 0.6508765264568908 1103 44-45 0.7898208486368432 1103 46-47 0.824208207365686 1103 48-49 0.7876518909127483 1103 50-51 0.8503799003371029 1103 52-53 0.911093549553577 1103 54-55 1.0105798525368854 1103 56-57 0.9283711644101231 1103 58-59 1.2211661638799072 1103 60-61 1.3502439751462347 1103 62-63 1.2946272803001975 1103 64-65 1.224710297822483 1103 66-67 1.3303406487795932 1103 68-69 1.3312628376577287 1103 70-71 1.3110886017498622 1103 72-73 1.3727911354903775 1103 74-75 1.492231763523531 1103 76-77 1.4751341330059127 1103 78-79 1.7441900234295886 1103 80-81 2.1146683824606924 1103 82-83 2.1449176022702225 1103 84-85 2.243759027657333 1103 86-87 2.512561860772326 1103 88-89 2.751402242987748 1103 90-91 2.9502598513416167 1103 92-93 3.056474912651513 1103 94-95 2.972217946738912 1103 96-97 3.556245076083375 1103 98-99 4.100348305405383 1103 100-101 4.560721489851389 1104 1 0.39182614558916384 1104 2 0.4135424366207445 1104 3 0.32317016246218344 1104 4 0.3101232657779178 1104 5 0.23229157842584414 1104 6 0.28159949444467003 1104 7 0.2971512115116681 1104 8 0.3279795493845654 1104 9 0.35645119654645896 1104 10-11 0.47978547663536375 1104 12-13 0.46432273025673965 1104 14-15 0.5663722149235966 1104 16-17 0.6703925875896459 1104 18-19 0.7106562605789577 1104 20-21 0.6428106557285531 1104 22-23 0.6319504966823786 1104 24-25 0.6201645565342702 1104 26-27 0.6422263417339735 1104 28-29 0.6444427252581093 1104 30-31 0.7484697567353962 1104 32-33 0.7432332790668923 1104 34-35 0.8940735204667263 1104 36-37 0.9698319535670805 1104 38-39 0.8763682973640883 1104 40-41 1.0531676676598138 1104 42-43 1.0628482867312172 1104 44-45 1.146601997576397 1104 46-47 1.038759727615961 1104 48-49 1.1932795029295846 1104 50-51 1.306039894728471 1104 52-53 1.4380392353821492 1104 54-55 1.5188328754297515 1104 56-57 1.611625101755422 1104 58-59 1.661847068750312 1104 60-61 1.6034999637338743 1104 62-63 1.7209365789546212 1104 64-65 1.871517029913747 1104 66-67 2.0550470736865556 1104 68-69 1.9641621826228963 1104 70-71 1.9923235715585896 1104 72-73 2.2624158298660255 1104 74-75 2.42879350842788 1104 76-77 2.3876524130894268 1104 78-79 2.6547897464401515 1104 80-81 2.925478931584003 1104 82-83 3.0595328473040233 1104 84-85 3.1193635524397116 1104 86-87 3.25210769707315 1104 88-89 3.20804751505349 1104 90-91 3.5880601234539746 1104 92-93 3.93416438898943 1104 94-95 4.135231644877802 1104 96-97 4.480729055952509 1104 98-99 5.00688552864008 1104 100-101 5.25978756495698 1105 1 0.28321525971591655 1105 2 0.21717548079966065 1105 3 0.2408228609817371 1105 4 0.07565524360431652 1105 5 0.08508240300039915 1105 6 0.10025173908868368 1105 7 0.1661540622580162 1105 8 0.13684440156902866 1105 9 0.2042034926976939 1105 10-11 0.27042544114127764 1105 12-13 0.3202435111266553 1105 14-15 0.33367405235455294 1105 16-17 0.42783767629936875 1105 18-19 0.34583008470983856 1105 20-21 0.34734890111712247 1105 22-23 0.28579747433028757 1105 24-25 0.2647608877920149 1105 26-27 0.3358516853279525 1105 28-29 0.5854899028152474 1105 30-31 0.550121812689575 1105 32-33 0.5467531093480389 1105 34-35 0.5622568453855195 1105 36-37 0.5458851742984834 1105 38-39 0.6362589592574182 1105 40-41 0.6086710360282552 1105 42-43 0.6285276466137191 1105 44-45 0.5634091616702577 1105 46-47 0.686572455509932 1105 48-49 0.6757160495997212 1105 50-51 0.8318814768671885 1105 52-53 0.8355036520500931 1105 54-55 0.8489225285974484 1105 56-57 0.8387538545156517 1105 58-59 1.0377809559137958 1105 60-61 0.8669915415378284 1105 62-63 1.0607149449006954 1105 64-65 1.1079137681295492 1105 66-67 1.2165729624411519 1105 68-69 1.0466652807059091 1105 70-71 1.0030992907473255 1105 72-73 1.141693351807593 1105 74-75 1.390450205131927 1105 76-77 1.382609549899751 1105 78-79 1.5723837981207645 1105 80-81 1.9195665652605456 1105 82-83 1.9834826864076973 1105 84-85 2.053142601748956 1105 86-87 2.1338743948481387 1105 88-89 2.392615491839539 1105 90-91 2.608416696234702 1105 92-93 2.8477653558973124 1105 94-95 3.236831683635714 1105 96-97 3.6335999462115254 1105 98-99 4.022877246686477 1105 100-101 4.481843111163041 1106 1 0.26684917313411205 1106 2 0.2109195426616779 1106 3 0.33488365209377946 1106 4 0.1775699263224766 1106 5 0.14076592391249676 1106 6 0.19957947118902553 1106 7 0.1924975057397944 1106 8 0.17284633090165613 1106 9 0.24034866555373213 1106 10-11 0.25938566621865533 1106 12-13 0.19145259098406342 1106 14-15 0.19181121830886383 1106 16-17 0.19059161870895736 1106 18-19 0.4216603102926868 1106 20-21 0.6020307454288911 1106 22-23 0.49521667838634187 1106 24-25 0.4095340841187465 1106 26-27 0.30597264557359694 1106 28-29 0.40741258729482155 1106 30-31 0.45948190309062653 1106 32-33 0.3689192735486415 1106 34-35 0.42213195279673954 1106 36-37 0.5249483171059381 1106 38-39 0.5956573878622535 1106 40-41 0.5839819181667707 1106 42-43 0.7963928640534519 1106 44-45 0.2857179696905163 1106 46-47 0.09726423091573366 1106 48-49 0.6619442814246312 1106 50-51 0.493105832035738 1106 52-53 0.6931367241696051 1106 54-55 0.8181269701747382 1106 56-57 0.682809119786036 1106 58-59 1.0383468192344552 1106 60-61 1.0068976279311457 1106 62-63 1.1007352320217123 1106 64-65 0.7176192022165466 1106 66-67 1.090901701187061 1106 68-69 0.9415484369981293 1106 70-71 1.1498318048853413 1106 72-73 1.5890318237453123 1106 74-75 1.7508073886832527 1106 76-77 1.7458776954358584 1106 78-79 1.8602581310574386 1106 80-81 1.7940153304276976 1106 82-83 2.1281836612689737 1106 84-85 2.1067341846498593 1106 86-87 2.4433253832027475 1106 88-89 2.5457785435776934 1106 90-91 2.5828162856946513 1106 92-93 2.4285911136872507 1106 94-95 2.742131951017914 1106 96-97 3.399690554593839 1106 98-99 3.783889221056853 1106 100-101 4.0200478380279705 1107 1 0.22606760251611746 1107 2 0.2113859500548827 1107 3 0.27087072085979713 1107 4 0.18933929558065188 1107 5 0.15113251062926025 1107 6 0.14346698274536607 1107 7 0.12765975736004975 1107 8 0.19259209733480276 1107 9 0.1780850587410967 1107 10-11 0.28419308031603663 1107 12-13 0.29248023007255597 1107 14-15 0.4042603793096049 1107 16-17 0.34660548513130607 1107 18-19 0.4130709374978849 1107 20-21 0.3347680346406392 1107 22-23 0.36799249071255247 1107 24-25 0.3284729538937654 1107 26-27 0.31281447412792573 1107 28-29 0.34764004694108763 1107 30-31 0.3845410347658671 1107 32-33 0.49718308686225043 1107 34-35 0.45390640150438344 1107 36-37 0.49347293878782494 1107 38-39 0.6221073155275789 1107 40-41 0.6943424522685078 1107 42-43 0.6119417946987866 1107 44-45 0.5732130296885529 1107 46-47 0.7077885416593688 1107 48-49 0.8230227624793471 1107 50-51 0.7549692541842745 1107 52-53 0.9351392615207743 1107 54-55 0.9771285735243254 1107 56-57 0.9954978931345977 1107 58-59 1.042838809013844 1107 60-61 1.1280763213021103 1107 62-63 1.1703889631028446 1107 64-65 1.2809936419046082 1107 66-67 1.5004340951387256 1107 68-69 1.5341878637324093 1107 70-71 1.4860148122279924 1107 72-73 1.6417720561894704 1107 74-75 1.6009576952897433 1107 76-77 1.4724158013945967 1107 78-79 1.5491762676590248 1107 80-81 1.6476185945673762 1107 82-83 1.8712537137605736 1107 84-85 2.1413450549090918 1107 86-87 2.4000367244978413 1107 88-89 2.626210676554834 1107 90-91 2.7636785103748416 1107 92-93 2.9394620849895468 1107 94-95 3.0534915226351806 1107 96-97 3.452582102413654 1107 98-99 3.5900500710813574 1107 100-101 4.100760052798488 1108 1 0.11178371735788772 1108 2 0.06907068621467971 1108 3 0.15280511462978552 1108 4 0.09016971945808194 1108 5 0.04196332319628482 1108 6 0.005958894572280826 1108 7 0.06948893057156624 1108 8 0.07210289718938157 1108 9 0.22877704053213677 1108 10-11 0.16609432115038913 1108 12-13 0.14821580225793696 1108 14-15 0.3771927221704061 1108 16-17 0.32338128211645056 1108 18-19 0.27925997325046836 1108 20-21 0.35032304907931433 1108 22-23 0.22072372100655713 1108 24-25 0.2818462079344215 1108 26-27 0.267930543698796 1108 28-29 0.3016460647705088 1108 30-31 0.2622352823885663 1108 32-33 0.28941020962186315 1108 34-35 0.3349376635794812 1108 36-37 0.3685189527760713 1108 38-39 0.38018291336646115 1108 40-41 0.33869376363858805 1108 42-43 0.3634959814572696 1108 44-45 0.4931669814041584 1108 46-47 0.4594976326515976 1108 48-49 0.4730763787787069 1108 50-51 0.5029537843385867 1108 52-53 0.5723352548170126 1108 54-55 0.6083175978448665 1108 56-57 0.6340971701719553 1108 58-59 0.757249704211695 1108 60-61 0.7573053212037948 1108 62-63 0.6878514145452215 1108 64-65 0.8349106040896856 1108 66-67 0.8329957653150295 1108 68-69 0.9507916541418417 1108 70-71 0.9816083298001921 1108 72-73 1.0709619584911962 1108 74-75 1.2105658733187212 1108 76-77 1.2261569387003384 1108 78-79 1.5280721377178104 1108 80-81 1.7277252326996546 1108 82-83 1.8768599894969675 1108 84-85 2.094705162095959 1108 86-87 2.377443570464731 1108 88-89 2.7207422713024947 1108 90-91 2.779794266707622 1108 92-93 2.9323982232898764 1108 94-95 3.155529970894886 1108 96-97 3.4668830213215713 1108 98-99 3.7748868328855174 1108 100-101 3.9796832454490527 1109 1 0.21081336833170639 1109 2 0.2796170690442139 1109 3 0.3064799874472328 1109 4 0.1872524638987514 1109 5 0.22165903459688252 1109 6 0.24310861756447366 1109 7 0.24753355128817134 1109 8 0.24271194915436922 1109 9 0.22421015510781928 1109 10-11 0.34449154705509244 1109 12-13 0.385057657644154 1109 14-15 0.46380942882699827 1109 16-17 0.4318633450850484 1109 18-19 0.42724751246432646 1109 20-21 0.570913475601948 1109 22-23 0.3882093593296858 1109 24-25 0.40424677181055557 1109 26-27 0.45276541637015555 1109 28-29 0.5566236229384955 1109 30-31 0.5545296447228125 1109 32-33 0.6560564248224097 1109 34-35 0.7306344559861486 1109 36-37 0.6843902034927893 1109 38-39 0.6048831715662217 1109 40-41 0.6202076468972422 1109 42-43 0.7762156977662258 1109 44-45 0.8141582201421542 1109 46-47 0.9296134755989485 1109 48-49 0.7861529001303964 1109 50-51 0.762759897135723 1109 52-53 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2316 66-67 0.41469005448638185 2316 68-69 0.3708190929048065 2316 70-71 0.46876036041836855 2316 72-73 0.675635871386735 2316 74-75 0.7831247965650405 2316 76-77 0.8461339845962961 2316 78-79 0.9663834084346874 2316 80-81 1.0488519913460657 2316 82-83 1.2565920192168498 2316 84-85 1.4173804550959446 2316 86-87 1.5244704593635987 2316 88-89 1.7945628876674853 2316 90-91 1.942999783858891 2316 92-93 2.078128712301446 2316 94-95 2.465484042271054 2316 96-97 2.781820236096582 2316 98-99 2.9742030414908918 2316 100-101 3.309583500918091 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 27.0 5 66.0 6 154.0 7 455.0 8 1054.0 9 1749.0 10 2922.0 11 4540.0 12 6541.0 13 9090.0 14 12210.0 15 15943.0 16 19949.0 17 24791.0 18 24301.0 19 21432.0 20 21759.0 21 23113.0 22 26183.0 23 28452.0 24 33614.0 25 38820.0 26 46558.0 27 54808.0 28 65700.0 29 77843.0 30 91805.0 31 109092.0 32 130499.0 33 157881.0 34 196764.0 35 250686.0 36 314304.0 37 341596.0 38 172415.0 39 8476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24711284161978 18.666116344358475 14.622489029590243 23.464281784431503 2 16.40328362096144 20.29560127753485 38.371537154058096 24.929577947445615 3 19.798295941085804 23.522050008563113 29.304803904778215 27.37485014557287 4 13.662553096566496 16.399961808577913 35.23612531753042 34.701359777325166 5 14.263262088169478 37.17614909238813 33.40233695109443 15.158251868347955 6 33.79666835502438 35.256805201911455 16.408225199231204 14.538301243832963 7 30.25223036620521 32.094838035402425 19.290821256866 18.362110341526364 8 27.646522118699114 34.27050744497245 18.846696120873283 19.23627431545515 9 26.96665486386171 16.41464752357314 19.116348112243394 37.502349500321756 10-11 25.843647229430687 26.14451171710261 26.377749129453935 21.634091924012772 12-13 26.79875389781568 23.192276255853415 26.93724061837564 23.071729227955267 14-15 23.070658840564942 24.762256256735412 25.18210500671561 26.984979895984036 16-17 22.966424516495312 28.082212602056085 26.06016519074517 22.89119769070343 18-19 23.120346223223727 26.259663992553527 28.28922552334451 22.33076426087824 20-21 23.407852276264414 25.4341170138932 28.50495139799015 22.653079311852228 22-23 23.247336983211618 25.353324173671638 28.509725325750995 22.88961351736575 24-25 23.26220649824379 25.635139523786382 28.386031757993074 22.716622219976756 26-27 22.5324526564825 26.107415456092838 28.345947566184577 23.014184321240084 28-29 22.70445790658772 26.541026021973767 28.188673331897583 22.565842739540923 30-31 22.71317085994495 25.909690131413598 28.039290067704144 23.33784894093731 32-33 22.41070667827259 25.987898555781864 28.026934533430325 23.574460232515225 34-35 23.433115731780767 26.0832598395491 27.783990580805373 22.69963384786476 36-37 23.508350897174193 25.450653010936502 27.878669807998406 23.1623262838909 38-39 22.77618472699525 25.920662145794797 27.98854031134538 23.314612815864574 40-41 23.25065828390807 25.7144031598484 27.983408612166887 23.05152994407664 42-43 22.968037047797026 25.79757517706825 27.690064032092902 23.54432374304182 44-45 22.950228309622485 26.02712569649474 27.972697470322107 23.04994852356067 46-47 23.480408160207055 26.410893977035904 27.112918401140607 22.995779461616436 48-49 23.18358471024399 26.423561709759753 27.094587564753088 23.298266015243172 50-51 23.36024144514286 26.509021439003117 27.188182238162906 22.942554877691116 52-53 23.76891535076809 26.182146537746355 26.97793674695571 23.071001364529845 54-55 22.9521241409606 26.40052697312 27.339064044702805 23.308284841216594 56-57 23.144087415541083 26.596857428253003 27.10839556208825 23.150659594117666 58-59 23.172324234897797 25.995792093091165 27.04691251038811 23.78497116162293 60-61 22.672795847581973 26.452376538307238 27.529079214232897 23.34574839987789 62-63 22.810062326516963 26.467426185015174 27.52100849330986 23.201502995157995 64-65 22.405241815496897 26.463166043201692 27.315001736168348 23.816590405133063 66-67 22.45642819174614 26.504191538057594 27.208293720003674 23.831086550192595 68-69 23.054700135618404 25.997801419122947 27.445889114027565 23.501609331231087 70-71 22.80398252613993 26.315923553788895 27.053548912208107 23.826545007863064 72-73 22.812459994271286 26.299824927438436 27.008999389022875 23.878715689267395 74-75 22.659822752411262 26.831443528716136 26.778630614877613 23.730103103994985 76-77 22.79340709872354 26.35747599228122 27.124815304655797 23.724301604339438 78-79 22.923844506108274 26.523535892015893 27.086302766351988 23.466316835523845 80-81 23.00442819358174 26.70281008861739 26.88381263506954 23.40894908273133 82-83 22.910035115421906 26.66192409734013 26.901885545946016 23.526155241291953 84-85 22.715615711986732 26.655151662157646 26.937125120985687 23.692107504869934 86-87 23.024055129952078 26.728928346262 26.58226195076368 23.664754573022247 88-89 22.70743162038379 26.877557425262278 26.388561231134943 24.02644972321899 90-91 22.609080436713512 26.76922214018222 26.720454841759967 23.901242581344302 92-93 22.7731954326832 26.361082799261602 26.900645638615778 23.96507612943942 94-95 22.47715044037878 26.219759950642295 27.061405555653078 24.241684053325844 96-97 22.701230196491053 25.93309435145749 27.03210413587072 24.33357131618073 98-99 22.39871245241593 26.288371782361985 26.61783702110333 24.695078744118756 100-101 22.3944587639901 25.897764152801443 27.09836528827957 24.60941179492888 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 458.0 1 335.5 2 280.0 3 369.0 4 353.0 5 342.5 6 577.0 7 1308.0 8 2027.0 9 2236.5 10 1988.0 11 1663.0 12 1669.5 13 1806.0 14 1980.0 15 2182.5 16 2285.0 17 2414.0 18 2634.5 19 2871.0 20 3189.0 21 3594.5 22 4238.0 23 5096.5 24 6114.0 25 7546.5 26 9431.0 27 11848.5 28 14226.5 29 17003.0 30 20481.5 31 25455.0 32 33413.0 33 36236.5 34 35029.0 35 37944.5 36 42829.0 37 47619.0 38 51939.0 39 55046.5 40 58400.0 41 64937.5 42 70440.5 43 74290.5 44 78391.0 45 83468.0 46 86446.5 47 90221.5 48 95517.0 49 94330.5 50 91318.5 51 88910.0 52 87324.0 53 89376.0 54 91780.0 55 86878.0 56 78835.5 57 72914.5 58 67935.5 59 60119.5 60 48524.0 61 40136.5 62 34231.5 63 25800.5 64 18369.0 65 14274.0 66 10950.0 67 8748.5 68 6716.0 69 4753.5 70 3503.5 71 2560.0 72 1899.0 73 1572.0 74 1278.0 75 997.0 76 694.5 77 402.5 78 219.0 79 129.5 80 75.5 81 38.0 82 25.5 83 17.5 84 11.0 85 7.5 86 3.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.563099122582049E-5 2 0.04739675364349164 3 1.2844648683873073E-4 4 0.0 5 0.1024146655060813 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001434319103032493 26-27 0.011902707780389047 28-29 0.0 30-31 0.0 32-33 5.994169385807434E-4 34-35 0.015905956620196157 36-37 0.0032325699187747228 38-39 0.012266639493098785 40-41 0.007471303984452836 42-43 0.005715868664323517 44-45 0.002932861449484352 46-47 2.1407747806455123E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.9266973025809607E-4 68-69 2.3548522587100635E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1407747806455123E-5 82-83 8.349021644517496E-4 84-85 0.004260141813484569 86-87 0.0013058726161937625 88-89 8.563099122582049E-5 90-91 9.205331556775701E-4 92-93 3.6393171270973705E-4 94-95 0.0 96-97 2.1407747806455123E-5 98-99 0.0 100-101 9.847563990969354E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2335603.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.82803205802924 #Duplication Level Percentage of deduplicated Percentage of total 1 73.54325603405145 34.438859512144134 2 13.81114236552775 12.934972349018786 3 4.989343482231458 7.009234096033618 4 2.3564704476925202 4.413954946733754 5 1.3076929072225125 3.061834269073664 6 0.8117458814958666 2.2807477296996983 7 0.5560611003261384 1.8227472922606796 8 0.4057737480420107 1.5201268865294364 9 0.3020720874590274 1.2730897255830431 >10 1.6967974777268486 14.745768458711689 >50 0.13767446755803697 4.450928353860187 >100 0.07290761893704788 6.456165396547994 >500 0.004943117306870903 1.6263792974439644 >1k 0.0039361860036193665 3.422336531702639 >5k 1.8307841877299378E-4 0.5428551546567076 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7046 0.30167798208856555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5618 0.24053745435332974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4066 0.17408780516209305 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3660 0.15670471394325147 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3646 0.15610529700467074 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3322 0.14223307642608782 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2960 0.12673386701421432 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2891 0.12377959781692352 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2859 0.12240950195731039 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2534 0.10849446588311455 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2446 0.10472670226917846 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2437 0.10434136280866227 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2844648683873073E-4 2 0.0 0.0 0.0 0.0 1.2844648683873073E-4 3 0.0 0.0 0.0 0.0 1.2844648683873073E-4 4 0.0 0.0 0.0 4.2815495612910245E-5 1.2844648683873073E-4 5 0.0 0.0 0.0 4.2815495612910245E-5 1.2844648683873073E-4 6 0.0 0.0 0.0 4.2815495612910245E-5 1.2844648683873073E-4 7 0.0 0.0 0.0 4.2815495612910245E-5 1.2844648683873073E-4 8 0.0 0.0 0.0 4.2815495612910245E-5 1.2844648683873073E-4 9 0.0 0.0 0.0 4.2815495612910245E-5 1.2844648683873073E-4 10-11 0.0 0.0 0.0 4.2815495612910245E-5 1.2844648683873073E-4 12-13 0.0 0.0 0.0 4.2815495612910245E-5 4.7097045174201264E-4 14-15 0.0 0.0 0.0 4.2815495612910245E-5 5.351936951613781E-4 16-17 0.0 0.0 0.0 4.2815495612910245E-5 5.994169385807434E-4 18-19 0.0 0.0 0.0 8.563099122582049E-5 5.994169385807434E-4 20-21 0.0 0.0 0.0 1.4985423464518587E-4 7.706789210323844E-4 22-23 0.0 0.0 0.0 2.783007214839166E-4 8.134944166452945E-4 24-25 0.0 0.0 0.0 5.994169385807435E-4 8.134944166452945E-4 26-27 0.0 0.0 0.0 0.0014985423464518584 8.349021644517497E-4 28-29 0.0 0.0 0.0 0.003939025596387743 8.563099122582048E-4 30-31 0.0 0.0 0.0 0.012266639493098783 8.777176600646599E-4 32-33 0.0 0.0 0.0 0.02997084692903717 0.0010061641469033909 34-35 0.0 0.0 0.0 0.05741557961691263 0.001070387390322756 36-37 0.0 0.0 0.0 0.10500500299066237 0.001070387390322756 38-39 0.0 0.0 0.0 0.18057435274744893 0.0011132028859356664 40-41 0.0 0.0 0.0 0.26468539387901113 0.0011132028859356664 42-43 0.0 0.0 0.0 0.3474691546465731 0.0011132028859356664 44-45 0.0 0.0 0.0 0.4529879435845904 0.001198833877161487 46-47 0.0 4.2815495612910245E-5 0.0 0.5792080246514497 0.0013058726161937623 48-49 0.0 4.2815495612910245E-5 0.0 0.7122999927641813 0.0015413578420647688 50-51 0.0 4.2815495612910245E-5 0.0 0.8627750520957542 0.0015413578420647688 52-53 0.0 4.2815495612910245E-5 0.0 1.0102102112388107 0.0018410663113551404 54-55 0.0 4.2815495612910245E-5 0.0 1.1707255042916112 0.0018838818069680506 56-57 0.0 4.2815495612910245E-5 0.0 1.3505505858658342 0.001926697302580961 58-59 0.0 4.2815495612910245E-5 0.0 1.544761673965995 0.0019695127981938713 60-61 0.0 4.2815495612910245E-5 0.0 1.7590318217608045 0.0020123282938067817 62-63 0.0 4.2815495612910245E-5 0.0 1.9771125486651626 0.002097959285032602 64-65 0.0 4.2815495612910245E-5 0.0 2.202193609102232 0.002140774780645512 66-67 0.0 4.2815495612910245E-5 0.0 2.4681848755974367 0.0021621825284519674 68-69 0.0 4.2815495612910245E-5 0.0 2.760015293695033 0.0021835902762584224 70-71 0.0 4.2815495612910245E-5 0.0 3.068051376882116 0.0022049980240648774 72-73 0.0 4.2815495612910245E-5 0.0 3.3970028296761052 0.002461890997742339 74-75 0.0 4.2815495612910245E-5 0.0 3.721843994891255 0.0027187839714198003 76-77 0.0 4.2815495612910245E-5 0.0 4.074814940724087 0.0027830072148391657 78-79 0.0 4.2815495612910245E-5 0.0 4.474411961279378 0.002890045953871441 80-81 0.0 4.2815495612910245E-5 0.0 4.893404401347318 0.002932861449484352 82-83 0.0 4.2815495612910245E-5 0.0 5.327467895871002 0.0031255311797424475 84-85 0.0 4.2815495612910245E-5 0.0 5.7695592958221065 0.0032111621709682682 86-87 0.0 4.2815495612910245E-5 0.0 6.235263441603731 0.0034038319012263643 88-89 4.2815495612910245E-5 4.2815495612910245E-5 0.0 6.726036060066715 0.0036393171270973704 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6745 0.0 32.507942 1 GTATCAA 9815 0.0 31.547226 1 TAAGGTG 1475 0.0 28.362057 5 GTAAGGT 1335 0.0 27.775406 4 AGGTAAG 1275 0.0 27.232227 2 ATCAACG 12335 0.0 24.896626 3 TATCAAC 12485 0.0 24.72445 2 GGTAAGG 1465 0.0 24.661701 3 GTACATG 9750 0.0 24.391352 1 TCAACGC 12725 0.0 24.133583 4 CAACGCA 12845 0.0 23.908125 5 AAGGTAA 1295 0.0 23.873388 1 TACATGG 9860 0.0 23.73333 2 AACGCAG 13220 0.0 23.244486 6 ACATGGG 10165 0.0 22.1208 3 GAGTACT 7210 0.0 21.178385 12-13 ACGCAGA 14635 0.0 20.867258 7 CGCAGAG 14725 0.0 20.675205 8 AGTACTT 7370 0.0 20.428614 12-13 GTACTTT 7350 0.0 20.354963 14-15 >>END_MODULE