FastQCFastQC Report
Thu 26 May 2016
SRR938552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346505
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66420.28305927325959246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51290.2185803993598991No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36520.15563572206323872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.1488170704942031No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33650.14340476581128103No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33310.14195580235286095No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30650.1306197941193392No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28660.12213909623035109No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27130.11561876066746077No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25180.10730852906769855No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24920.10620049818773027No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG23630.10070296036019527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.037.3757671
GTATCAA87550.037.091211
TATCAAC107950.030.0251432
ATCAACG109450.029.3965443
TCAACGC111800.028.7361284
CAACGCA112500.028.5144735
AACGCAG115500.027.808466
GTACATG97850.026.4130461
TACATGG98650.025.9665972
TAAGGTG13750.025.2309885
AGGTAAG11550.025.0998552
GTAAGGT12450.024.812324
ACGCAGA128850.024.776597
ACATGGG102100.024.390963
CGCAGAG130800.024.2982528
GGACCGA7900.023.4557726
AAGGTAA12800.022.7247921
GCAGAGT139950.022.1999659
GAGTACT66000.020.69296812-13
AGAGTAC115350.020.42623510-11
Produced by FastQC (version 0.11.5)