##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938552_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2346505 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.065132612118873 31.0 31.0 34.0 28.0 34.0 2 31.22055823448064 31.0 31.0 34.0 28.0 34.0 3 31.27925446568407 31.0 31.0 34.0 27.0 34.0 4 34.94942819214108 37.0 35.0 37.0 32.0 37.0 5 34.93939113703146 37.0 35.0 37.0 32.0 37.0 6 34.91754588206716 37.0 35.0 37.0 32.0 37.0 7 34.832058742683266 37.0 35.0 37.0 32.0 37.0 8 34.786573222729125 37.0 35.0 37.0 32.0 37.0 9 36.26123745741006 39.0 35.0 39.0 32.0 39.0 10-11 36.09241148005225 38.5 35.0 39.0 31.0 39.0 12-13 35.902024074101696 38.0 35.0 39.0 30.0 39.0 14-15 37.050041870782294 39.0 36.0 41.0 31.0 41.0 16-17 36.980723672014335 39.0 36.0 41.0 31.0 41.0 18-19 36.973255543883354 39.0 36.0 41.0 31.0 41.0 20-21 36.89543150344875 39.0 36.0 41.0 30.5 41.0 22-23 36.75199030046814 39.0 36.0 41.0 30.0 41.0 24-25 36.599502877684046 39.0 36.0 41.0 30.0 41.0 26-27 36.380924609152764 39.0 35.5 41.0 29.5 41.0 28-29 36.155325047251125 39.0 35.0 40.0 29.0 41.0 30-31 35.87198045603994 38.5 35.0 40.0 27.5 41.0 32-33 35.575664445632974 38.0 34.5 40.0 27.0 41.0 34-35 35.26278976605633 38.0 34.0 40.0 26.0 41.0 36-37 35.04270521477687 38.0 34.0 40.0 25.0 41.0 38-39 34.80085510152333 38.0 33.5 40.0 25.0 41.0 40-41 34.5227774924835 38.0 33.0 40.0 24.0 41.0 42-43 34.09605668856448 38.0 33.0 40.0 23.5 41.0 44-45 33.62806983151538 38.0 32.5 40.0 21.5 41.0 46-47 33.13112543974975 37.0 31.5 40.0 18.5 41.0 48-49 32.6166948291182 37.0 31.0 40.0 15.5 41.0 50-51 31.86669024783668 36.0 30.5 38.5 13.0 40.5 52-53 31.856833034662188 36.0 30.5 39.0 10.5 40.0 54-55 31.831816680552567 36.0 30.5 39.0 8.5 40.0 56-57 31.395323257355088 36.0 30.0 39.0 7.0 40.0 58-59 30.929168912915166 35.0 29.0 39.0 2.0 40.0 60-61 30.361319281228894 35.0 28.0 38.5 2.0 40.0 62-63 29.62907941811332 34.5 27.5 38.0 2.0 40.0 64-65 28.954332081116384 34.0 26.0 38.0 2.0 40.0 66-67 28.244833486397855 34.0 26.0 37.0 2.0 39.0 68-69 27.40075750957275 33.0 24.0 36.0 2.0 39.0 70-71 26.556777206952468 32.0 21.5 36.0 2.0 39.0 72-73 25.666553448639572 31.5 19.0 35.0 2.0 38.0 74-75 24.73444782772677 31.0 13.0 35.0 2.0 37.0 76-77 23.79627445924897 30.5 7.0 34.0 2.0 36.5 78-79 22.817200261665754 29.5 2.0 34.0 2.0 36.0 80-81 21.858141363431997 29.0 2.0 34.0 2.0 35.0 82-83 20.866518929216006 27.5 2.0 33.5 2.0 35.0 84-85 19.897030051075962 26.0 2.0 33.0 2.0 35.0 86-87 18.918328535417565 25.0 2.0 32.5 2.0 34.5 88-89 17.818503476446885 22.5 2.0 32.0 2.0 34.0 90-91 16.66246097920098 19.0 2.0 31.0 2.0 34.0 92-93 15.512982925670306 4.5 2.0 31.0 2.0 34.0 94-95 14.2585894766898 2.0 2.0 30.5 2.0 34.0 96-97 12.845497878760114 2.0 2.0 29.5 2.0 34.0 98-99 11.147074052686868 2.0 2.0 28.0 2.0 33.5 100-101 7.861546214476423 2.0 2.0 9.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5112518007306122 1101 2 0.4669111873866747 1101 3 0.35393921521724536 1101 4 0.3513355849862094 1101 5 0.38086780370294804 1101 6 0.43478072677035584 1101 7 0.229405942377376 1101 8 0.4376863579963697 1101 9 0.48685107701628993 1101 10-11 0.5602023315636373 1101 12-13 0.4955731477347456 1101 14-15 0.6051523903590663 1101 16-17 0.5689602773046261 1101 18-19 0.5212192256706984 1101 20-21 0.7164697409513607 1101 22-23 0.7314749581427051 1101 24-25 0.6991419388463811 1101 26-27 0.7358726898080334 1101 28-29 0.7578582166158512 1101 30-31 0.8800453048438257 1101 32-33 0.9262975696134887 1101 34-35 1.1142565609121249 1101 36-37 1.033183396924052 1101 38-39 1.0921650157617577 1101 40-41 1.0852584349290382 1101 42-43 1.1751375906160888 1101 44-45 1.2887319517260991 1101 46-47 1.4186948979313811 1101 48-49 1.1910163119549324 1101 50-51 1.1990080288661282 1101 52-53 1.3223441515247139 1101 54-55 1.5231800173048633 1101 56-57 1.7177886452877758 1101 58-59 1.625230253152072 1101 60-61 1.78071417773711 1101 62-63 1.7773666811702071 1101 64-65 1.8613657166847126 1101 66-67 2.035318572410965 1101 68-69 2.0903630512077633 1101 70-71 2.509110239303414 1101 72-73 2.6147748650493483 1101 74-75 2.732227020543437 1101 76-77 2.967274884359867 1101 78-79 3.024168319079447 1101 80-81 3.3351853984300988 1101 82-83 3.5094620114502746 1101 84-85 3.4451726520775843 1101 86-87 3.5120657480713113 1101 88-89 3.9887348308355293 1101 90-91 3.8084189819497567 1101 92-93 3.6567350276963637 1101 94-95 3.7501492917713684 1101 96-97 3.66173265740362 1101 98-99 2.9656876126555485 1101 100-101 2.116329452361968 1102 1 0.766082467321894 1102 2 0.5974705435993357 1102 3 0.6472214752575773 1102 4 0.5671675519676072 1102 5 0.5225339992137776 1102 6 0.5940935930940228 1102 7 0.5617048059494962 1102 8 0.545363559707603 1102 9 0.5960269312218287 1102 10-11 0.6772236843462807 1102 12-13 0.7158414238697546 1102 14-15 0.9041966978542177 1102 16-17 0.8839423733827232 1102 18-19 0.9313263665086069 1102 20-21 0.806098752843333 1102 22-23 0.8006367719042302 1102 24-25 0.8494806998949898 1102 26-27 0.8150378958915994 1102 28-29 0.738852072743704 1102 30-31 0.5956007975786051 1102 32-33 0.8157703462290797 1102 34-35 0.7819461069063607 1102 36-37 1.1545317507055515 1102 38-39 1.2586996645621937 1102 40-41 1.2095579204268674 1102 42-43 1.307574031693342 1102 44-45 1.2393240061540212 1102 46-47 1.3123578518900345 1102 48-49 1.4530048722911388 1102 50-51 1.5358219788482295 1102 52-53 1.5311819042998174 1102 54-55 1.524980397998771 1102 56-57 1.882193387000143 1102 58-59 1.8751717355968047 1102 60-61 1.770107611709335 1102 62-63 1.8760827815075558 1102 64-65 1.915069190045564 1102 66-67 2.159175454113157 1102 68-69 2.2357012468693576 1102 70-71 2.1530732253006626 1102 72-73 2.412431430134813 1102 74-75 2.3954216455976187 1102 76-77 2.3590840285523953 1102 78-79 2.821015721126148 1102 80-81 3.1069228327038587 1102 82-83 3.466129431966287 1102 84-85 3.5699383933941853 1102 86-87 3.5012703427830587 1102 88-89 3.92934328148667 1102 90-91 3.4986061250492106 1102 92-93 3.9414447014680576 1102 94-95 3.8429348593256947 1102 96-97 3.7421440706826754 1102 98-99 3.395377874806657 1102 100-101 2.4991646797417753 1103 1 0.4892120911984854 1103 2 0.4823160959260626 1103 3 0.5379405021002306 1103 4 0.5123191312680362 1103 5 0.47464940127625965 1103 6 0.5439175959678906 1103 7 0.532328085956749 1103 8 0.4576642971451861 1103 9 0.6294947023495041 1103 10-11 0.684058384509683 1103 12-13 0.7338896290289796 1103 14-15 0.737601144840184 1103 16-17 0.7887814924898393 1103 18-19 0.84747209817737 1103 20-21 0.7620341310607515 1103 22-23 0.810765793697449 1103 24-25 0.9208603872331835 1103 26-27 0.9931495534903547 1103 28-29 0.9716264857582289 1103 30-31 0.947947685577347 1103 32-33 1.1377678334245047 1103 34-35 0.9747147831752017 1103 36-37 1.1170730007479435 1103 38-39 1.2340075564364525 1103 40-41 1.4062387640034046 1103 42-43 1.4320996275680145 1103 44-45 1.4664445172192515 1103 46-47 1.499892618310092 1103 48-49 1.3893548541003398 1103 50-51 1.407309802926246 1103 52-53 1.5353471848917515 1103 54-55 1.5885968754719677 1103 56-57 1.610951619825876 1103 58-59 1.7503571030463654 1103 60-61 1.90058686824176 1103 62-63 2.215993852785246 1103 64-65 2.455422155695654 1103 66-67 2.471528702018464 1103 68-69 2.667843518162446 1103 70-71 2.5435692888484063 1103 72-73 2.911276841333933 1103 74-75 2.91110651314386 1103 76-77 3.0999079388685473 1103 78-79 3.257285333510918 1103 80-81 3.2403733752662056 1103 82-83 3.5648871424365822 1103 84-85 3.560349138887034 1103 86-87 3.791780335809154 1103 88-89 3.753968308177196 1103 90-91 3.6394677915829945 1103 92-93 3.829289490422717 1103 94-95 3.7691864275375266 1103 96-97 3.881641656392498 1103 98-99 3.3855428383196706 1103 100-101 2.3555959290601924 1104 1 0.6391913479582243 1104 2 0.5407837983978396 1104 3 0.7146173050948796 1104 4 0.6422779998910073 1104 5 0.5591420320128009 1104 6 0.720460152522449 1104 7 0.7088055113352141 1104 8 0.6974156207717783 1104 9 0.7452045672109762 1104 10-11 0.7956459309437918 1104 12-13 0.8393963313907662 1104 14-15 0.852283288244827 1104 16-17 0.9389831652870981 1104 18-19 0.9005146854786901 1104 20-21 0.8927822821408213 1104 22-23 0.8921198292753516 1104 24-25 0.9811249999581904 1104 26-27 1.0427382830253507 1104 28-29 1.0373132688930653 1104 30-31 0.9969996891838164 1104 32-33 1.1545957546053884 1104 34-35 1.156173184533948 1104 36-37 1.3844470451081534 1104 38-39 1.2941347181289231 1104 40-41 1.2602163994125917 1104 42-43 1.4065329560222821 1104 44-45 1.3063030647766993 1104 46-47 1.1891017162346458 1104 48-49 1.2843605400866593 1104 50-51 1.4159389908738653 1104 52-53 1.595838977237623 1104 54-55 1.9206541458694169 1104 56-57 2.0961303797871835 1104 58-59 2.1540073936682553 1104 60-61 2.197131451484509 1104 62-63 2.353100521542114 1104 64-65 2.411581772114598 1104 66-67 2.6283152060073185 1104 68-69 2.842491900752673 1104 70-71 2.729470034825564 1104 72-73 2.9814753441829893 1104 74-75 2.762079540002677 1104 76-77 2.847383802657781 1104 78-79 3.200214486900901 1104 80-81 3.3934843849466745 1104 82-83 3.5542314395178884 1104 84-85 3.6909251946919817 1104 86-87 3.8018998324629543 1104 88-89 4.017774515212924 1104 90-91 3.6034915644622707 1104 92-93 3.6689581009200936 1104 94-95 3.798545084657391 1104 96-97 3.9123200859343346 1104 98-99 3.3388166759403735 1104 100-101 2.2802156002343645 1105 1 0.49552473687246135 1105 2 0.4016704697059801 1105 3 0.3936896405289936 1105 4 0.35332130854234123 1105 5 0.32251941637986903 1105 6 0.48196139218593004 1105 7 0.5295737870552841 1105 8 0.4083901831713135 1105 9 0.49120170928386386 1105 10-11 0.5376597565421548 1105 12-13 0.627017015353168 1105 14-15 0.6068664405546258 1105 16-17 0.5761810184870768 1105 18-19 0.6359817526363472 1105 20-21 0.6942585583216214 1105 22-23 0.7615031076419783 1105 24-25 0.7128768775488723 1105 26-27 0.7007208052823657 1105 28-29 0.9289730595574284 1105 30-31 0.7758435755350206 1105 32-33 0.8118765197511735 1105 34-35 0.7318385978484798 1105 36-37 0.9837491053969742 1105 38-39 1.2247923900536293 1105 40-41 1.1988321936325832 1105 42-43 1.2905657962389725 1105 44-45 1.4176918233433327 1105 46-47 1.4220544222837717 1105 48-49 1.3350238009494362 1105 50-51 1.1042020061009872 1105 52-53 1.3905269741916335 1105 54-55 1.7206370915107918 1105 56-57 1.7969064522576303 1105 58-59 1.7723278731601404 1105 60-61 2.0589361992394686 1105 62-63 2.0233823431657534 1105 64-65 2.2335498021329165 1105 66-67 2.539731116038304 1105 68-69 2.7700924301649152 1105 70-71 2.863829708935281 1105 72-73 2.7550774940898073 1105 74-75 3.0960955159044694 1105 76-77 3.144636811504398 1105 78-79 3.374887227475188 1105 80-81 3.298205550314627 1105 82-83 3.587067111683538 1105 84-85 3.741915991783287 1105 86-87 4.02364904778894 1105 88-89 4.084112086741666 1105 90-91 3.8034544976731404 1105 92-93 3.9566378636926416 1105 94-95 3.9084549725332494 1105 96-97 3.7229401919985623 1105 98-99 3.597992276176953 1105 100-101 2.4526088076419716 1106 1 0.31115834278668686 1106 2 0.40864625443258973 1106 3 0.26448370932533294 1106 4 0.39370047730640323 1106 5 0.3938503311308281 1106 6 -0.08439846867864986 1106 7 0.3379431223855036 1106 8 0.04331821496507615 1106 9 0.43889496161605024 1106 10-11 0.39554162453397623 1106 12-13 0.5226576662231608 1106 14-15 0.5434254500908864 1106 16-17 0.1543991233021842 1106 18-19 0.5940331224606226 1106 20-21 0.48213613672543687 1106 22-23 0.44837662413214474 1106 24-25 0.5998428734258212 1106 26-27 0.457361921827534 1106 28-29 0.4858204270993909 1106 30-31 0.3215690756686911 1106 32-33 0.45547879675692826 1106 34-35 0.4660546104854717 1106 36-37 0.6095202518610776 1106 38-39 0.6990301112513961 1106 40-41 0.9487102837709713 1106 42-43 0.37310995957179927 1106 44-45 1.0087096030873326 1106 46-47 1.2101008304791279 1106 48-49 0.9646347597403846 1106 50-51 0.6480876712879073 1106 52-53 0.8717712136945615 1106 54-55 0.9037285748018142 1106 56-57 1.2445988850277203 1106 58-59 1.340445612761986 1106 60-61 1.5016426620930652 1106 62-63 1.524410565769962 1106 64-65 1.44286104378752 1106 66-67 1.601539620765294 1106 68-69 1.7136015721342126 1106 70-71 1.6675092640900857 1106 72-73 1.8035009926397585 1106 74-75 1.9182367726767175 1106 76-77 2.3286259611579148 1106 78-79 2.5891906677182135 1106 80-81 2.2736900713272945 1106 82-83 2.593261280040281 1106 84-85 2.7237971949463393 1106 86-87 2.3290852523378724 1106 88-89 2.0726946032857576 1106 90-91 2.2123279620391543 1106 92-93 2.4917533129462583 1106 94-95 2.271195614404448 1106 96-97 2.357470162482855 1106 98-99 2.3800699775478673 1106 100-101 1.5056508664212922 1107 1 0.24835210884426928 1107 2 0.15094405284370893 1107 3 0.20678070785675118 1107 4 0.1323729009738841 1107 5 0.060549419587921705 1107 6 0.21528397908120667 1107 7 0.2793066613092492 1107 8 0.27529676539109715 1107 9 0.380972953086129 1107 10-11 0.3586771689480912 1107 12-13 0.30564846440712046 1107 14-15 0.28315307505609155 1107 16-17 0.4059561691224971 1107 18-19 0.30961189941256606 1107 20-21 0.479506372242021 1107 22-23 0.5412900899468909 1107 24-25 0.44349318841845076 1107 26-27 0.6074526281852997 1107 28-29 0.5089931019291001 1107 30-31 0.4716800189828234 1107 32-33 0.4768761385967153 1107 34-35 0.5455472147977787 1107 36-37 0.4808862384166943 1107 38-39 0.7462372512975364 1107 40-41 0.6439557988455036 1107 42-43 0.8893056837348823 1107 44-45 0.584671698388199 1107 46-47 0.6118222515773937 1107 48-49 0.2999772842247026 1107 50-51 0.632227816610051 1107 52-53 0.7227258701142354 1107 54-55 0.8417027834808231 1107 56-57 0.9720697134151308 1107 58-59 0.9895215917347464 1107 60-61 1.0495193814344788 1107 62-63 1.3680376842513446 1107 64-65 1.3345118994988177 1107 66-67 1.432595190007678 1107 68-69 1.562851924881162 1107 70-71 1.7388586131479862 1107 72-73 1.7827156387569794 1107 74-75 2.008329382748162 1107 76-77 2.1091132958627767 1107 78-79 2.3629461348959495 1107 80-81 2.6352744090429 1107 82-83 2.790317882727237 1107 84-85 2.6763760070929905 1107 86-87 2.523640551220918 1107 88-89 2.9670299352518263 1107 90-91 2.5422669792109716 1107 92-93 2.5127278383776357 1107 94-95 2.6827990692448367 1107 96-97 3.048470076095848 1107 98-99 2.9183818954354184 1107 100-101 1.9676628998114696 1108 1 0.3222919963229458 1108 2 0.2511764338432734 1108 3 0.1731812949245395 1108 4 0.1613808926508966 1108 5 0.18639619365661986 1108 6 0.24265400208707888 1108 7 0.1623914610219117 1108 8 0.2980283026355295 1108 9 0.3204611575442513 1108 10-11 0.38371153842304295 1108 12-13 0.3582839993478686 1108 14-15 0.47976830104312995 1108 16-17 0.404125607537658 1108 18-19 0.3667039321967209 1108 20-21 0.5215432590487765 1108 22-23 0.47958921894388595 1108 24-25 0.570641280602949 1108 26-27 0.5036438091638118 1108 28-29 0.501531984332857 1108 30-31 0.7217746898563888 1108 32-33 0.6675384012228491 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0.3633729985098402 2316 98-99 0.44063392677274393 2316 100-101 0.5551469020999287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7222.0 3 2780.0 4 3050.0 5 3618.0 6 4481.0 7 5685.0 8 7398.0 9 9771.0 10 12331.0 11 15464.0 12 19136.0 13 22677.0 14 25644.0 15 29356.0 16 34344.0 17 38935.0 18 39475.0 19 39580.0 20 43611.0 21 47506.0 22 53065.0 23 59312.0 24 65348.0 25 72274.0 26 79887.0 27 88518.0 28 97606.0 29 107299.0 30 119587.0 31 134174.0 32 148722.0 33 168077.0 34 188858.0 35 201871.0 36 193019.0 37 127705.0 38 28797.0 39 322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37581634613204 17.837919702985843 13.98538630793143 23.800877642950685 2 15.928955307612656 19.467889509338214 39.307508639942775 25.295646543106354 3 19.710986700408757 23.255696962359035 28.78988896827982 28.243427368952386 4 13.118740961060931 15.678849905858597 35.524949177286 35.67745995579447 5 14.348846214204325 37.02588000900649 33.157652786211884 15.467620990577302 6 33.842557631520705 34.731777787916684 16.733107311484176 14.69255726907843 7 30.341840623875555 31.937639729944966 19.294669670309805 18.425849975869674 8 27.686764678295955 33.75662738129548 19.062599655862194 19.49400828454637 9 27.184877442568855 16.3734340594338 19.117978713653233 37.32370978434411 10-11 25.883714951647374 25.86619264054407 26.421918492689016 21.828173915119535 12-13 26.85572034742781 23.06239764749365 26.85853394229189 23.22334806278665 14-15 23.217543766912947 24.59438767245451 25.16844741064593 27.019621149986616 16-17 23.133182051368944 27.89694411710733 25.95027047672148 23.019603354802243 18-19 23.28400895034638 26.105247991010433 28.178010265871666 22.432732792771517 20-21 23.564010145564854 25.353040972981756 28.313663280903782 22.769285600549612 22-23 23.29848272045807 25.322992899495816 28.276083018197077 23.102441361849035 24-25 23.200779882510265 25.543134746532854 28.273853364437112 22.98223200651977 26-27 22.472084476340523 25.96280658687626 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23.14979953972881 52-53 23.717208043919943 26.124988225987728 26.95078867768359 23.20701505240874 54-55 22.849413386779013 26.343158345613265 27.3164749292239 23.49095333838382 56-57 23.049682040650975 26.60716991352946 27.056389649960224 23.286758395859337 58-59 23.1113636964169 25.871227521967676 27.056680824513446 23.96072795710198 60-61 22.66098744764168 26.283641871653284 27.60591851451542 23.44945216618961 62-63 22.87681164361959 26.25289189553203 27.5795836011409 23.290712859707476 64-65 22.441144054396624 26.325073881589994 27.35788182525672 23.87590023875666 66-67 22.52164635231288 26.39313266236549 27.20671544611847 23.87850553920316 68-69 23.060860485434826 25.839516696145076 27.51520446651182 23.584418351908283 70-71 22.85356438747416 26.08303431820608 27.137239791839868 23.926161502479886 72-73 22.911665316635137 26.05923132806235 27.10741839661433 23.921684958688182 74-75 22.770558213151894 26.6294207742318 26.94062486747241 23.6593961451439 76-77 23.01087170080384 26.071070723377886 27.0229465382014 23.89511103761688 78-79 23.14880955932683 26.285484985982027 26.900246944730238 23.6654585099609 80-81 23.215777852555096 26.43298779252037 26.68980453924825 23.661429815676286 82-83 23.113811300360204 26.365425737999715 26.73984399274904 23.78091896889104 84-85 23.061865011409445 26.355835693785366 26.710734594267915 23.87156470053727 86-87 23.39344766371092 26.333873938261082 26.3911733726609 23.88150502536709 88-89 23.14634945282445 26.38903777705364 26.265982152885208 24.198630617236695 90-91 23.077019810453105 26.312830169778 26.417397476189407 24.19275254357948 92-93 23.319259008423924 25.779243293882175 26.66711277154948 24.234384926144426 94-95 23.07285229475958 25.65726614368838 26.762025271671426 24.50785628988061 96-97 23.352574398215875 25.324570422126968 26.718822995843176 24.604032183813977 98-99 23.0863752633756 25.637264000862665 26.130226834934383 25.14613390082735 100-101 24.009812533083057 24.472148573937602 26.51018216722739 25.00785672575195 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 402.0 1 303.0 2 265.0 3 349.5 4 311.0 5 290.0 6 493.0 7 1062.0 8 1696.5 9 1951.5 10 1731.0 11 1450.5 12 1490.0 13 1647.0 14 1791.0 15 1945.5 16 2129.5 17 2282.5 18 2457.5 19 2716.0 20 3123.0 21 3650.5 22 4212.5 23 5000.0 24 6041.5 25 7524.0 26 9495.0 27 11868.0 28 14158.0 29 16985.0 30 20424.5 31 25304.5 32 32163.0 33 35068.0 34 35436.5 35 37934.5 36 42216.5 37 47259.0 38 51317.0 39 54602.5 40 58642.5 41 64671.5 42 69735.0 43 73232.0 44 76849.5 45 81618.0 46 85268.0 47 88577.5 48 92834.0 49 93014.0 50 90449.0 51 88274.0 52 87546.0 53 88542.5 54 89206.0 55 86032.5 56 80096.5 57 74556.0 58 69719.0 59 62442.5 60 51834.0 61 43208.5 62 36869.0 63 28964.0 64 21795.0 65 17211.0 66 13354.0 67 10408.5 68 8032.5 69 5957.0 70 4450.5 71 3273.0 72 2482.0 73 1998.5 74 1550.5 75 1217.5 76 856.5 77 534.0 78 327.0 79 202.5 80 128.0 81 72.5 82 38.0 83 25.5 84 17.0 85 8.0 86 4.5 87 5.0 88 5.5 89 5.5 90 4.5 91 3.0 92 1.5 93 2.5 94 3.0 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38815174056735446 2 0.03042823262682159 3 0.03703380133432488 4 0.04675037982020068 5 0.06516073905659694 6 0.055572010287640555 7 0.038738464226583794 8 0.03976126196193914 9 0.037076417906631354 10-11 0.03937771281118089 12-13 0.03202635408831432 14-15 0.028510486873030313 16-17 0.023630889343939178 18-19 0.028723569734562675 20-21 0.03620277817434866 22-23 0.03522259701129979 24-25 0.043618061755674925 26-27 0.03841883993428524 28-29 0.03345400926058116 30-31 0.03643716932203426 32-33 0.030747856919120135 34-35 0.032452519811379046 36-37 0.03443419042363004 38-39 0.016194297476459674 40-41 0.013552069993458355 42-43 0.014084777147289267 44-45 0.012017873390425335 46-47 0.0205411878517199 48-49 0.01821858466101713 50-51 0.011911331959659153 52-53 0.010057511064327585 54-55 0.00841677303052838 56-57 0.00914125475973842 58-59 0.004389506947566701 60-61 0.007372667009019797 62-63 0.012486655685796535 64-65 0.007798832732084526 66-67 0.003984649510655209 68-69 0.006605568707503287 70-71 0.014084777147289267 72-73 0.011229466802755586 74-75 0.012593197116562718 76-77 0.005881086978293249 78-79 0.009844428202795222 80-81 0.011911331959659153 82-83 0.014148702005748975 84-85 0.008651164178213981 86-87 0.0025356860522351326 88-89 0.017536719504113563 90-91 0.02759423056844115 92-93 0.011527782808900898 94-95 0.022096692740906157 96-97 0.020498571279413427 98-99 0.012081798248885044 100-101 0.12663514460868397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2346505.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.87549928992646 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01525697692236 37.91298895376068 2 12.611211723685065 12.579809627395333 3 4.517633127559851 6.759576235372789 4 2.1168000707941723 4.223058417112441 5 1.1987047029490443 2.989299778038329 6 0.747164219217465 2.235911309102348 7 0.49251107715878545 1.7194965115379743 8 0.33480683246631726 1.3358926347949067 9 0.2724166632610253 1.2228225385535463 >10 1.5046886284663832 13.875850781660612 >50 0.11807160421771475 4.053214146323412 >100 0.06288905965635225 5.9137354993632325 >500 0.00405197517944056 1.4089411580119915 >1k 0.0036209139901381302 3.2632637215442615 >5k 1.7242447572086332E-4 0.5061386874281545 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6642 0.28305927325959246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5129 0.2185803993598991 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3652 0.15563572206323872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3492 0.1488170704942031 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3365 0.14340476581128103 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3331 0.14195580235286095 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3065 0.1306197941193392 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2866 0.12213909623035109 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2713 0.11561876066746077 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2518 0.10730852906769855 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2492 0.10620049818773027 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2363 0.10070296036019527 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.523314461294563E-5 2 0.0 0.0 0.0 0.0 8.523314461294563E-5 3 0.0 0.0 0.0 0.0 8.523314461294563E-5 4 0.0 0.0 0.0 0.0 8.523314461294563E-5 5 0.0 0.0 0.0 0.0 8.523314461294563E-5 6 0.0 0.0 0.0 0.0 1.7046628922589126E-4 7 0.0 0.0 0.0 0.0 1.7046628922589126E-4 8 0.0 0.0 0.0 0.0 1.7046628922589126E-4 9 0.0 0.0 0.0 0.0 1.7046628922589126E-4 10-11 0.0 0.0 0.0 0.0 2.130828615323641E-4 12-13 0.0 0.0 0.0 0.0 8.097148738229836E-4 14-15 0.0 0.0 0.0 0.0 8.949480184359292E-4 16-17 0.0 0.0 0.0 0.0 0.0010227977353553476 18-19 0.0 0.0 0.0 4.2616572306472815E-5 0.0010654143076618205 20-21 0.0 0.0 0.0 6.392485845970922E-5 0.0011506474522747661 22-23 0.0 0.0 0.0 2.3439114768560048E-4 0.0011506474522747661 24-25 0.0 0.0 0.0 4.900905815244374E-4 0.0011506474522747661 26-27 0.0 0.0 0.0 9.588728768956384E-4 0.0011719557384280025 28-29 0.0 0.0 0.0 0.0033027843537516434 0.0012358805968877117 30-31 0.0 0.0 0.0 0.011378624805828243 0.0012358805968877117 32-33 0.0 0.0 0.0 0.028744878020715917 0.0012784971691941845 34-35 0.0 0.0 0.0 0.055678551718406744 0.00136373031380713 36-37 0.0 0.0 0.0 0.10302556355089804 0.00136373031380713 38-39 0.0 0.0 0.0 0.1757507441918939 0.001512888316879785 40-41 0.0 0.0 0.0 0.25514541839885274 0.0015341966030330213 42-43 0.0 0.0 0.0 0.336734846079595 0.0015341966030330213 44-45 0.0 0.0 0.0 0.44016526706740455 0.0015555048891862577 46-47 0.0 0.0 0.0 0.5651170570699828 0.0015981214614927308 48-49 0.0 0.0 0.0 0.6965678743492982 0.0016407380337992035 50-51 0.0 0.0 0.0 0.844724387972751 0.0019603623260977497 52-53 0.0 0.0 0.0 0.9852312268671918 0.0021734451876301137 54-55 0.0 0.0 0.0 1.1421880626719312 0.0021734451876301137 56-57 0.0 0.0 0.0 1.3285290250819837 0.002216061759936587 58-59 0.0 0.0 0.0 1.5299562540885274 0.0022586783322430595 60-61 0.0 0.0 0.0 1.749751225759161 0.0023012949045495322 62-63 0.0 0.0 0.0 1.9670957445221724 0.002343911476856005 64-65 0.0 0.0 0.0 2.1895755602481133 0.002343911476856005 66-67 0.0 0.0 0.0 2.4539900831236245 0.0023865280491624776 68-69 4.2616572306472815E-5 0.0 0.0 2.747533033170609 0.002429144621468951 70-71 4.2616572306472815E-5 0.0 0.0 3.0585487778632476 0.002429144621468951 72-73 4.2616572306472815E-5 0.0 0.0 3.377917370727955 0.00249306947992866 74-75 4.2616572306472815E-5 0.0 0.0 3.69555999241425 0.002620919196848078 76-77 4.2616572306472815E-5 0.0 0.0 4.034319125678403 0.00272746062761426 78-79 4.2616572306472815E-5 0.0 0.0 4.410793925433783 0.00272746062761426 80-81 4.2616572306472815E-5 0.0 0.0 4.810835689674644 0.002770077199920733 82-83 4.2616572306472815E-5 0.0 0.0 5.2207218821182995 0.0028553103445336787 84-85 6.392485845970922E-5 0.0 0.0 5.634422257783385 0.0028553103445336787 86-87 8.523314461294563E-5 0.0 0.0 6.048037400303857 0.002897926916840152 88-89 8.523314461294563E-5 0.0 0.0 6.482342888679121 0.0029405434891466246 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5435 0.0 37.375767 1 GTATCAA 8755 0.0 37.09121 1 TATCAAC 10795 0.0 30.025143 2 ATCAACG 10945 0.0 29.396544 3 TCAACGC 11180 0.0 28.736128 4 CAACGCA 11250 0.0 28.514473 5 AACGCAG 11550 0.0 27.80846 6 GTACATG 9785 0.0 26.413046 1 TACATGG 9865 0.0 25.966597 2 TAAGGTG 1375 0.0 25.230988 5 AGGTAAG 1155 0.0 25.099855 2 GTAAGGT 1245 0.0 24.81232 4 ACGCAGA 12885 0.0 24.77659 7 ACATGGG 10210 0.0 24.39096 3 CGCAGAG 13080 0.0 24.298252 8 GGACCGA 790 0.0 23.455772 6 AAGGTAA 1280 0.0 22.724792 1 GCAGAGT 13995 0.0 22.199965 9 GAGTACT 6600 0.0 20.692968 12-13 AGAGTAC 11535 0.0 20.426235 10-11 >>END_MODULE