FastQCFastQC Report
Thu 26 May 2016
SRR938552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346505
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.296270410674599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56650.24142288211616852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39860.16986965721360064No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37630.1603661615892572No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36010.15346227687560862No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33480.142680284082071No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30600.13040671125780684No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29320.12495179000257829No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28220.1202639670488663No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25430.10837394337536038No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24920.10620049818773027No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG24240.10330257127089011No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24030.10240762325245417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61800.036.98151
GTATCAA96800.034.948821
ATCAACG122850.027.1812363
TATCAAC124300.026.9035192
TCAACGC124350.026.8527834
GTGTAAG8900.026.6936441
CAACGCA127200.026.1764475
AACGCAG130550.025.504746
TAAGGTA8200.024.9077434
TAAGGTG14500.024.2406065
AGGTAAG14350.024.1645372
GTACATG105700.023.7348691
GTAAGGT14900.023.5898534
TACATGG105750.023.2229672
ACGCAGA147250.022.6121857
CGCAGAG148750.022.2245038
AAGGTAA14600.021.8046861
ACATGGG108550.021.572783
GAGTACT73100.021.08516912-13
AGTACTT76500.021.01730512-13
Produced by FastQC (version 0.11.5)