##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938552_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2346505 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80573320747239 31.0 31.0 34.0 30.0 34.0 2 32.06111088619031 33.0 31.0 34.0 30.0 34.0 3 32.117871046513855 33.0 31.0 34.0 30.0 34.0 4 35.77337529645153 37.0 35.0 37.0 35.0 37.0 5 35.667841321454674 37.0 35.0 37.0 33.0 37.0 6 35.69995972733917 37.0 35.0 37.0 33.0 37.0 7 35.621075599668444 37.0 35.0 37.0 33.0 37.0 8 35.58836098793738 37.0 35.0 37.0 33.0 37.0 9 37.13073571119601 39.0 37.0 39.0 33.0 39.0 10-11 37.062401102916894 39.0 37.0 39.0 33.0 39.0 12-13 36.94203805233741 39.0 37.0 39.0 33.0 39.0 14-15 37.95390740697335 40.0 37.0 41.0 33.0 41.0 16-17 38.04470478434949 40.0 37.0 41.0 33.0 41.0 18-19 38.1818012320451 40.0 37.5 41.0 33.0 41.0 20-21 38.12983373144314 40.0 37.0 41.0 33.0 41.0 22-23 38.00904920296355 40.0 37.0 41.0 33.0 41.0 24-25 37.877410659683235 40.0 37.0 41.0 32.5 41.0 26-27 37.65823959463116 40.0 37.0 41.0 32.0 41.0 28-29 37.51925161037373 39.5 36.5 41.0 32.0 41.0 30-31 37.259470574322236 39.0 36.0 41.0 31.0 41.0 32-33 37.1562807238851 39.0 36.0 41.0 31.0 41.0 34-35 37.0171030958809 39.0 36.0 41.0 31.0 41.0 36-37 36.88342662811287 39.0 35.5 41.0 30.5 41.0 38-39 36.67019801790322 39.0 35.0 40.0 30.0 41.0 40-41 36.44825794106554 39.0 35.0 40.0 30.0 41.0 42-43 36.17343048491267 38.5 35.0 40.0 30.0 41.0 44-45 35.871152842205746 38.0 35.0 40.0 29.0 41.0 46-47 35.44302569140062 38.0 34.0 40.0 27.5 41.0 48-49 35.0123758525978 38.0 34.0 40.0 26.0 41.0 50-51 34.59940528573347 38.0 33.0 40.0 25.5 41.0 52-53 33.980580054165664 37.0 33.0 40.0 23.5 41.0 54-55 33.53918359432433 37.0 32.0 40.0 23.0 41.0 56-57 33.13161553033128 36.0 32.0 39.0 22.0 40.0 58-59 32.581533599971024 36.0 31.0 39.0 19.5 40.0 60-61 32.071290067568576 35.0 31.0 39.0 18.0 40.0 62-63 31.55317738508974 35.0 30.0 38.0 15.0 40.0 64-65 30.89113042588872 34.5 29.0 38.0 10.0 40.0 66-67 30.233722067500388 34.0 28.5 37.5 7.5 39.5 68-69 29.492529101791817 34.0 27.5 36.5 4.5 39.0 70-71 28.70847409232028 33.0 26.0 36.0 2.0 39.0 72-73 28.128796230990346 33.0 26.0 35.5 2.0 38.0 74-75 27.416954364043544 32.0 25.0 35.0 2.0 37.5 76-77 26.290723011457466 30.5 24.0 34.0 2.0 36.0 78-79 26.795358842192964 32.0 25.0 34.5 2.0 36.0 80-81 26.421524778340554 32.0 24.0 35.0 2.0 36.0 82-83 25.751837306973563 31.5 22.0 34.0 2.0 35.5 84-85 24.982929079631194 31.0 19.5 34.0 2.0 35.0 86-87 24.08578865163296 30.5 15.0 34.0 2.0 35.0 88-89 23.10029064502313 29.5 6.5 34.0 2.0 35.0 90-91 22.243828161457145 29.0 2.0 34.0 2.0 35.0 92-93 21.16841344893789 29.0 2.0 33.5 2.0 35.0 94-95 19.987984470521052 26.5 2.0 33.0 2.0 34.5 96-97 18.603524177446886 25.0 2.0 32.5 2.0 34.0 98-99 16.8347135420551 12.5 2.0 32.0 2.0 34.0 100-101 12.607198365228285 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23373857180433433 1101 2 0.31089394482779653 1101 3 0.28069351999183567 1101 4 0.16018914408024898 1101 5 0.1219375475062563 1101 6 0.19548093466796956 1101 7 0.2842000628218173 1101 8 0.2428711490108384 1101 9 0.33205582489842556 1101 10-11 0.30637948920114155 1101 12-13 0.22716437855673632 1101 14-15 0.31906747725147966 1101 16-17 0.3623798248634813 1101 18-19 0.31867378292930226 1101 20-21 0.35205836163586923 1101 22-23 0.32802165845858156 1101 24-25 0.29348028217102495 1101 26-27 0.4896437444394337 1101 28-29 0.511427037831858 1101 30-31 0.319070801142864 1101 32-33 0.3246125194275322 1101 34-35 0.1820467000043564 1101 36-37 0.2850852397027026 1101 38-39 0.47506276128052605 1101 40-41 0.4921953064468809 1101 42-43 0.5681502725116871 1101 44-45 0.6120710948932029 1101 46-47 0.5010438378992674 1101 48-49 0.6770610295723642 1101 50-51 0.9109379489754375 1101 52-53 0.909412713165004 1101 54-55 1.0465167833275402 1101 56-57 1.1096389448987267 1101 58-59 1.0993569409871071 1101 60-61 1.0805462841231233 1101 62-63 1.1278749983427154 1101 64-65 1.0585469662188807 1101 66-67 1.33714232105212 1101 68-69 1.4837275527101497 1101 70-71 1.5504021583550731 1101 72-73 1.6375180449674254 1101 74-75 1.7530771438774835 1101 76-77 1.7826360654718663 1101 78-79 1.7417308375757052 1101 80-81 1.890256910759586 1101 82-83 2.0598313482975783 1101 84-85 2.4285710476128592 1101 86-87 2.4720898908153934 1101 88-89 2.2553254464178742 1101 90-91 2.588856138946184 1101 92-93 2.8095571185629105 1101 94-95 2.7625516844986144 1101 96-97 2.8859159613250362 1101 98-99 2.8923203329383114 1101 100-101 2.458986262481883 1102 1 0.18983476298052793 1102 2 0.2625000208535013 1102 3 0.19061983121854098 1102 4 0.18160826546883868 1102 5 0.2075269634614756 1102 6 0.22918610418983576 1102 7 0.2675970964246517 1102 8 0.33442615174354273 1102 9 0.2361479075957007 1102 10-11 0.303062457859852 1102 12-13 0.31549073878364453 1102 14-15 0.4836105503183248 1102 16-17 0.37956193601856825 1102 18-19 0.3530905862311613 1102 20-21 0.3646795900333757 1102 22-23 0.4349309703825881 1102 24-25 0.3354273807933623 1102 26-27 0.3738754777275304 1102 28-29 0.4703677852734742 1102 30-31 0.39795887336163105 1102 32-33 0.3747520757871925 1102 34-35 0.3060737631957764 1102 36-37 0.3330988466833418 1102 38-39 0.39483387377369183 1102 40-41 0.557823734105618 1102 42-43 0.5442126346986029 1102 44-45 0.4224882562735033 1102 46-47 0.41429471896069003 1102 48-49 0.452169866571289 1102 50-51 0.574351661494994 1102 52-53 0.6592125661643777 1102 54-55 0.6160576890775218 1102 56-57 0.8208809116916598 1102 58-59 0.908080956742559 1102 60-61 0.8434287213180554 1102 62-63 0.9028891330179043 1102 64-65 0.8803799508968915 1102 66-67 1.0799758522709055 1102 68-69 1.2920835957549457 1102 70-71 1.510046341465081 1102 72-73 1.4382233652591658 1102 74-75 1.4474446642785317 1102 76-77 1.5434134726070532 1102 78-79 1.5190695609318432 1102 80-81 1.9203748881817972 1102 82-83 1.913724037843572 1102 84-85 2.1771081087311934 1102 86-87 2.143959437186549 1102 88-89 2.171576836327599 1102 90-91 2.5271293527943968 1102 92-93 2.7982853747209155 1102 94-95 2.46090848139276 1102 96-97 2.611999713987064 1102 98-99 2.7368288231665403 1102 100-101 2.219318615811069 1103 1 0.2524884569040644 1103 2 0.2874018959262372 1103 3 0.24602121555209067 1103 4 0.20874875433854356 1103 5 0.2105247338285281 1103 6 0.26357267104079796 1103 7 0.18672511107992307 1103 8 0.11361294513180553 1103 9 0.2346911774124436 1103 10-11 0.3221810334607227 1103 12-13 0.40837726577063194 1103 14-15 0.5242958989814142 1103 16-17 0.46467783019485154 1103 18-19 0.5265246883434003 1103 20-21 0.4434275132122991 1103 22-23 0.388733580510241 1103 24-25 0.4167770006194118 1103 26-27 0.4778025825679393 1103 28-29 0.44564387761491986 1103 30-31 0.55197088387105 1103 32-33 0.5155625148315224 1103 34-35 0.5610245472329609 1103 36-37 0.5603724903691187 1103 38-39 0.6141311499191886 1103 40-41 0.7599579684562556 1103 42-43 0.6603237259540933 1103 44-45 0.6049799100416564 1103 46-47 0.7950961405006112 1103 48-49 0.7124549076861584 1103 50-51 0.7485521598404077 1103 52-53 0.8298438189651662 1103 54-55 0.8542187779961594 1103 56-57 0.938831885426346 1103 58-59 1.0406562332014673 1103 60-61 1.1475372759422342 1103 62-63 1.0795962043359104 1103 64-65 1.2493935513829548 1103 66-67 1.2781028872806353 1103 68-69 1.08013531077615 1103 70-71 1.3200151742572714 1103 72-73 1.429711720856755 1103 74-75 1.3308108510196845 1103 76-77 1.3944377016516647 1103 78-79 1.5000110103857018 1103 80-81 1.6007275422514518 1103 82-83 1.9142848826297758 1103 84-85 2.027989652264022 1103 86-87 1.7966261533395205 1103 88-89 1.8396792473186885 1103 90-91 2.146709342365618 1103 92-93 2.4068537450910874 1103 94-95 2.041445884333161 1103 96-97 2.29197993779346 1103 98-99 2.434257551432662 1103 100-101 1.9166206957937675 1104 1 0.17525327532926127 1104 2 0.22024524418835512 1104 3 0.14615849336340858 1104 4 0.153363003688753 1104 5 0.08969808755577446 1104 6 0.11570411476165532 1104 7 0.24816511634585225 1104 8 0.1664769413625109 1104 9 0.13473630153843175 1104 10-11 0.29207111594000423 1104 12-13 0.26104491575639344 1104 14-15 0.32408430926972187 1104 16-17 0.40964484454536176 1104 18-19 0.43562460493738797 1104 20-21 0.3606041286949875 1104 22-23 0.3949853939198178 1104 24-25 0.3232892521227484 1104 26-27 0.39574708768792277 1104 28-29 0.42785232682603436 1104 30-31 0.4836850896561202 1104 32-33 0.5173465201043754 1104 34-35 0.4765624900405143 1104 36-37 0.38306628809697685 1104 38-39 0.5160638656034138 1104 40-41 0.6161828026619034 1104 42-43 0.6002154453738413 1104 44-45 0.6195134296455294 1104 46-47 0.6937716044952822 1104 48-49 0.663678239197786 1104 50-51 0.767311584157035 1104 52-53 0.7987522568302836 1104 54-55 0.7902841274309864 1104 56-57 0.9449386260700194 1104 58-59 1.052386080862071 1104 60-61 0.9139131330624437 1104 62-63 0.9445498286866574 1104 64-65 1.013664077880172 1104 66-67 0.9800340439739443 1104 68-69 1.2385828329766113 1104 70-71 1.44726979991324 1104 72-73 1.5233395187041339 1104 74-75 1.576494221696624 1104 76-77 1.5989877032539574 1104 78-79 1.8191102081116561 1104 80-81 2.0061336896990767 1104 82-83 2.0318973226844115 1104 84-85 2.284623894806632 1104 86-87 2.355564411236461 1104 88-89 2.2412016019492818 1104 90-91 2.3892060489973055 1104 92-93 2.371823652235854 1104 94-95 2.5327212514971755 1104 96-97 2.645681025536067 1104 98-99 2.7356007953289954 1104 100-101 2.4697388941545775 1105 1 0.1714894180431763 1105 2 0.1980940781213718 1105 3 0.20421106738998418 1105 4 0.11403518999637896 1105 5 0.0038257562860977146 1105 6 0.1101153419500065 1105 7 0.11914244842611055 1105 8 0.09891408357421483 1105 9 0.19597202532987978 1105 10-11 0.12916067726785485 1105 12-13 0.16747069070925136 1105 14-15 0.24179298017165962 1105 16-17 0.12209885602962345 1105 18-19 0.24724648931511695 1105 20-21 0.26996731861361667 1105 22-23 0.25748751951756077 1105 24-25 0.2287756326805237 1105 26-27 0.2881308623165566 1105 28-29 0.30056773271233084 1105 30-31 0.3057916037105173 1105 32-33 0.3768098465405032 1105 34-35 0.39462365073834604 1105 36-37 0.343366980236226 1105 38-39 0.3904226539441211 1105 40-41 0.4433251449161091 1105 42-43 0.28029691298733184 1105 44-45 0.4117425086820745 1105 46-47 0.38242982804256087 1105 48-49 0.4512222446485694 1105 50-51 0.4056468077368649 1105 52-53 0.4266200372963951 1105 54-55 0.5152326049246057 1105 56-57 0.5626156009837544 1105 58-59 0.6174810969405939 1105 60-61 0.7082816086754562 1105 62-63 0.6689579209798246 1105 64-65 0.5902069974376367 1105 66-67 0.7432585670840055 1105 68-69 0.8768902936470617 1105 70-71 0.9921381318366684 1105 72-73 1.1095496320379503 1105 74-75 1.0802338690647098 1105 76-77 1.0745344466564255 1105 78-79 1.1168451017425305 1105 80-81 1.3268851093670193 1105 82-83 1.692981110649157 1105 84-85 1.8653903295813947 1105 86-87 1.875509748050952 1105 88-89 1.7851911882141813 1105 90-91 1.8326317672675643 1105 92-93 1.8961588338408113 1105 94-95 1.8807176700818644 1105 96-97 2.0735759574665344 1105 98-99 2.1909611768971295 1105 100-101 1.817686562392435 1106 1 0.016111590902262662 1106 2 0.060658268273549254 1106 3 0.08386296330755982 1106 4 0.09497987955363385 1106 5 0.11724839675453325 1106 6 -0.018891271752188743 1106 7 0.06275755977427622 1106 8 0.03685733348869036 1106 9 -0.0344496606765432 1106 10-11 0.09183052536530312 1106 12-13 0.07811317985702004 1106 14-15 0.10343155689389505 1106 16-17 0.11682923567209258 1106 18-19 0.1281780494876159 1106 20-21 0.07989054367569537 1106 22-23 0.18528090266824648 1106 24-25 0.0700975109398172 1106 26-27 -0.16467563792546258 1106 28-29 0.12514300695010405 1106 30-31 0.24547470199617294 1106 32-33 0.16977911837105353 1106 34-35 0.19362735827335342 1106 36-37 0.1908755688371926 1106 38-39 0.201383362662078 1106 40-41 0.17273573716610002 1106 42-43 0.37527948827763424 1106 44-45 0.26609181817642735 1106 46-47 -0.050355999565177 1106 48-49 0.22787370734562273 1106 50-51 0.3922036946072964 1106 52-53 0.2824846351479309 1106 54-55 0.09300601534703645 1106 56-57 0.45531549914496594 1106 58-59 0.8381643363548434 1106 60-61 0.7793699330463326 1106 62-63 0.6847726009432868 1106 64-65 0.8502088922652042 1106 66-67 1.0102386233682523 1106 68-69 0.9297576217877612 1106 70-71 0.845680500492417 1106 72-73 1.0240377524034265 1106 74-75 0.7355375258360368 1106 76-77 0.988694578271545 1106 78-79 1.1140876031181648 1106 80-81 1.1781845824377655 1106 82-83 1.61749083631058 1106 84-85 1.6651110719931914 1106 86-87 1.5653731090193723 1106 88-89 1.442938205458912 1106 90-91 1.3534701170038872 1106 92-93 1.492576216490587 1106 94-95 1.2930311643848427 1106 96-97 1.2691869893624173 1106 98-99 1.5406198046977586 1106 100-101 1.735497843140152 1107 1 0.006648534145998752 1107 2 0.05747730669016704 1107 3 0.06770139400758524 1107 4 0.04687863398096681 1107 5 0.002204579344670776 1107 6 -0.0950770934046119 1107 7 0.053844020370597434 1107 8 0.07544560981192916 1107 9 0.13372648563105116 1107 10-11 0.104041248872484 1107 12-13 0.19361764798501468 1107 14-15 0.26528281446476143 1107 16-17 0.10624322780768125 1107 18-19 0.022633043456522728 1107 20-21 0.08631717711002551 1107 22-23 0.06781624935211283 1107 24-25 0.1493447121607545 1107 26-27 0.18474644899266934 1107 28-29 0.18498232711635154 1107 30-31 0.12268428967418288 1107 32-33 0.23402025650387515 1107 34-35 0.2446622426267382 1107 36-37 0.18104552727545098 1107 38-39 0.31726577394742606 1107 40-41 0.4035647004900156 1107 42-43 0.35078293255961057 1107 44-45 0.4388581541194938 1107 46-47 0.3471770114699666 1107 48-49 0.4491510672306944 1107 50-51 0.4827267199442886 1107 52-53 0.5913127473998472 1107 54-55 0.5569447189660153 1107 56-57 0.6269309681784279 1107 58-59 0.5660224664920648 1107 60-61 0.8095534734418592 1107 62-63 1.1651824273053997 1107 64-65 1.1127082123674583 1107 66-67 0.9943453337190142 1107 68-69 1.25535713162148 1107 70-71 1.1482813093307662 1107 72-73 1.4236272541423638 1107 74-75 1.5184263393584843 1107 76-77 1.5034029261360224 1107 78-79 1.550724332954001 1107 80-81 1.4890039152101195 1107 82-83 1.6283524027309895 1107 84-85 1.8346734098175865 1107 86-87 2.142209712794166 1107 88-89 2.4237489315257115 1107 90-91 2.6104035542149333 1107 92-93 2.4597573924417198 1107 94-95 2.4134623315373034 1107 96-97 2.485412709014181 1107 98-99 2.4073631265021582 1107 100-101 2.107991740058056 1108 1 0.023777382520879797 1108 2 0.03334312330579792 1108 3 0.036808345001666964 1108 4 0.06517093552821507 1108 5 0.04702670957092181 1108 6 0.01746575232598957 1108 7 0.1254090080935768 1108 8 0.05107440090435489 1108 9 0.07875127190555276 1108 10-11 0.12133195889629889 1108 12-13 0.047755686324009616 1108 14-15 0.13997704999165705 1108 16-17 0.15934677591410917 1108 18-19 0.05009866624070014 1108 20-21 0.11280063796133533 1108 22-23 0.12671089917051148 1108 24-25 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-0.6301256577366843 2316 56-57 -0.659896507870485 2316 58-59 -0.7062871070015007 2316 60-61 -0.3133726940190513 2316 62-63 -0.5813068397596517 2316 64-65 -0.4641647186778606 2316 66-67 -0.4838468504687974 2316 68-69 0.0876195966084019 2316 70-71 -0.560485849971716 2316 72-73 -0.2755496284617145 2316 74-75 -0.21969087467518733 2316 76-77 -0.22092235901181567 2316 78-79 -0.2438121385212959 2316 80-81 -0.7300073187384335 2316 82-83 -0.20495144300311807 2316 84-85 -0.07620635683905164 2316 86-87 0.11133189727960868 2316 88-89 0.04425857645092535 2316 90-91 0.025005455090298057 2316 92-93 0.15804113149134835 2316 94-95 0.4170689680659869 2316 96-97 0.36771188192836135 2316 98-99 0.5917724265051341 2316 100-101 0.4436581212186219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 19.0 4 49.0 5 88.0 6 173.0 7 475.0 8 1102.0 9 2014.0 10 3270.0 11 5227.0 12 7527.0 13 10382.0 14 13920.0 15 18530.0 16 23263.0 17 29317.0 18 29803.0 19 27836.0 20 29063.0 21 31388.0 22 34629.0 23 38740.0 24 43947.0 25 49821.0 26 57488.0 27 67583.0 28 79664.0 29 93396.0 30 108470.0 31 126582.0 32 149096.0 33 177530.0 34 214398.0 35 259383.0 36 292330.0 37 245933.0 38 72870.0 39 1192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25716753250684 18.70022946505187 14.65728799846199 23.385315003979297 2 16.401931258582874 20.2926637576175 38.356699777772285 24.94870520602734 3 19.85931123362211 23.496200227065838 29.276875424043748 27.367613115268302 4 13.654179300704666 16.39357256856474 35.291081842996284 34.66116628773431 5 14.226970284691973 37.22346591797745 33.40441789711575 15.145145900214828 6 33.81509947773391 35.234359185256366 16.44624665193554 14.504294685074184 7 30.32501528869532 32.04455136468919 19.27756386626067 18.352869480354826 8 27.664071882316744 34.26577092199367 18.859434170522764 19.210723025166825 9 26.96363649536054 16.413886037125064 19.126179788544732 37.49629767896966 10-11 25.92956332929186 26.113496455366597 26.35796642240268 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23.543694750961812 25.432312903064275 27.872566432024172 23.151425913949737 38-39 22.810828827147 25.89890372552605 27.98773260296673 23.302534844360224 40-41 23.249452417779555 25.748468922178326 28.00712038028781 22.994958279754314 42-43 22.948625853946787 25.806213772721392 27.68947288903417 23.55568748429765 44-45 23.004700437910603 25.95573333458343 27.948928684135215 23.09063754337075 46-47 23.497125937138826 26.40123349459451 27.08258754300014 23.019053025266526 48-49 23.18584447934268 26.379743490851286 27.118075606060927 23.316336423745103 50-51 23.381454546229392 26.521315744053393 27.16050040379202 22.936729305925198 52-53 23.821534580152186 26.149827083257865 27.00232899567655 23.0263093409134 54-55 22.962874573035215 26.40569698338593 27.292547853083626 23.338880590495226 56-57 23.152987102094393 26.629988003434896 27.058732029124165 23.158292865346546 58-59 23.176831074299862 25.988587281936326 27.031862280284933 23.802719363478875 60-61 22.67828110317259 26.3995601969738 27.544390487128727 23.377768212724884 62-63 22.817637294614755 26.43650876516351 27.558517880848328 23.187336059373408 64-65 22.428396639595178 26.446482275965554 27.31786407262211 23.80725701181716 66-67 22.46585541310177 26.506738569745814 27.203288994805863 23.82411702234655 68-69 23.029791883836115 26.00940643807442 27.423248800968942 23.53755287712052 70-71 22.792093516121533 26.26197818925981 27.021064736931038 23.924863557687615 72-73 22.86977809702414 26.252563127570717 26.972785194620837 23.904873580784304 74-75 22.684481814443185 26.80271297099303 26.757305013200483 23.755500201363304 76-77 22.804191766052064 26.293721939650673 27.132543932358978 23.769542361938285 78-79 22.932595498411466 26.507784982346084 27.060884166025645 23.498735353216805 80-81 23.00392733607884 26.704585528711572 26.825084066641335 23.46640306856825 82-83 22.913232438772013 26.654800645734284 26.820154989865653 23.61181192562805 84-85 22.708321657414366 26.70332922980276 26.871547701571824 23.716801411211055 86-87 23.027420084508165 26.647496878729783 26.558850460524535 23.766232576237517 88-89 22.667989200960577 26.91505025559289 26.276419611294244 24.140540932152284 90-91 22.591959332990122 26.75906637709719 26.616213617720565 24.032760672192126 92-93 22.792655670244564 26.309588724096688 26.777755972163536 24.119999633495212 94-95 22.480107104037987 26.143728317473208 26.92013883635801 24.45602574213079 96-97 22.671057594166644 25.88287687433012 26.92022816912813 24.525837362375107 98-99 22.34131186594531 26.199390156850296 26.483173911839096 24.976124065365298 100-101 22.524690976580064 25.783857268576032 26.86987668894803 24.821575065895875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 463.0 1 330.5 2 259.0 3 348.0 4 331.0 5 327.5 6 533.0 7 1159.5 8 1878.0 9 2168.0 10 1914.5 11 1612.0 12 1624.5 13 1765.5 14 1981.0 15 2113.5 16 2236.0 17 2463.5 18 2679.0 19 2916.5 20 3228.5 21 3656.5 22 4297.5 23 5130.0 24 6098.5 25 7576.0 26 9594.5 27 12014.0 28 14486.0 29 17178.5 30 20493.5 31 25585.0 32 33302.0 33 36123.5 34 35450.0 35 37905.0 36 42646.0 37 47993.5 38 52157.0 39 55257.0 40 59022.5 41 65430.5 42 70724.0 43 74755.5 44 78411.5 45 83041.5 46 86475.0 47 90327.0 48 95356.5 49 94331.5 50 91294.5 51 89363.0 52 87979.0 53 89374.5 54 91515.0 55 87167.0 56 79430.5 57 73412.5 58 68410.0 59 60689.0 60 49320.0 61 40749.0 62 34589.5 63 26513.5 64 19201.0 65 14727.0 66 11195.5 67 8841.0 68 6866.5 69 4931.0 70 3626.0 71 2625.0 72 1974.0 73 1665.0 74 1351.5 75 1031.0 76 706.5 77 439.0 78 259.5 79 145.5 80 83.5 81 49.5 82 30.0 83 15.0 84 7.0 85 3.5 86 1.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02616657539617431 2 0.01120815851660235 3 0.0020882120430171683 4 0.0 5 1.704662892258913E-4 6 0.0 7 0.0 8 1.2784971691941845E-4 9 1.704662892258913E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017472794645653855 26-27 0.009119946473585184 28-29 2.3439114768560048E-4 30-31 0.0 32-33 2.3439114768560048E-4 34-35 0.010014894492021111 36-37 0.0011719557384280025 38-39 0.012188339679651225 40-41 0.012017873390425335 42-43 0.005135296962929975 44-45 9.162563045891656E-4 46-47 4.261657230647282E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.523314461294564E-5 66-67 5.540154399841466E-4 68-69 0.0011506474522747661 70-71 2.130828615323641E-5 72-73 2.770077199920733E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4915800307265488E-4 82-83 0.0012358805968877117 84-85 0.006456410704430632 86-87 0.0044534318060264096 88-89 0.0 90-91 0.001406346886113603 92-93 6.179402984438559E-4 94-95 6.392485845970922E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2346505.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.97940617607537 #Duplication Level Percentage of deduplicated Percentage of total 1 73.60770981541226 34.580464971089434 2 13.85539987299095 13.018369167303698 3 4.982379260573597 7.022076570172232 4 2.2965517732493193 4.315625542394637 5 1.2925995418102834 3.0362779448857116 6 0.8041462917994655 2.266698916045915 7 0.5541083861674162 1.822217805753007 8 0.4042143528969312 1.519180021355552 9 0.30238094465978355 1.2785109497169607 >10 1.6819680338521263 14.676632988833122 >50 0.1371419800976654 4.437610457041919 >100 0.07257266641062138 6.480120688163588 >500 0.004641042103700705 1.5286706583956364 >1k 0.004004036324761282 3.4781457827863425 >5k 1.820016511255128E-4 0.5393975360622355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6952 0.296270410674599 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5665 0.24142288211616852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3986 0.16986965721360064 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3763 0.1603661615892572 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3601 0.15346227687560862 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3348 0.142680284082071 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3060 0.13040671125780684 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2932 0.12495179000257829 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2822 0.1202639670488663 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2543 0.10837394337536038 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2492 0.10620049818773027 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2424 0.10330257127089011 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2403 0.10240762325245417 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.2616572306472815E-5 2 0.0 0.0 0.0 0.0 4.2616572306472815E-5 3 0.0 0.0 0.0 0.0 4.2616572306472815E-5 4 0.0 0.0 0.0 0.0 4.2616572306472815E-5 5 0.0 0.0 0.0 0.0 4.2616572306472815E-5 6 0.0 0.0 0.0 0.0 4.2616572306472815E-5 7 0.0 0.0 0.0 0.0 4.2616572306472815E-5 8 0.0 0.0 0.0 0.0 4.2616572306472815E-5 9 0.0 0.0 0.0 0.0 4.2616572306472815E-5 10-11 0.0 0.0 0.0 0.0 4.2616572306472815E-5 12-13 0.0 0.0 0.0 0.0 5.113988676776738E-4 14-15 0.0 0.0 0.0 0.0 6.81865156903565E-4 16-17 0.0 0.0 0.0 2.1308286153236408E-5 8.097148738229836E-4 18-19 0.0 0.0 0.0 8.523314461294563E-5 9.37564590742402E-4 20-21 0.0 0.0 0.0 1.0654143076618204E-4 0.0010654143076618205 22-23 0.0 0.0 0.0 2.770077199920733E-4 0.0010654143076618205 24-25 0.0 0.0 0.0 5.327071538309101E-4 0.0010654143076618205 26-27 0.0 0.0 0.0 0.0010227977353553476 0.0011293391661215298 28-29 0.0 0.0 0.0 0.0034945589291307715 0.0011506474522747661 30-31 0.0 0.0 0.0 0.011698249098126789 0.0012145723107344752 32-33 0.0 0.0 0.0 0.0293202017468533 0.00136373031380713 34-35 0.0 0.0 0.0 0.05633910858915706 0.001406346886113603 36-37 0.0 0.0 0.0 0.10368612042164838 0.001406346886113603 38-39 0.0 0.0 0.0 0.17647522592110393 0.001512888316879785 40-41 0.0 0.0 0.0 0.25682877300495843 0.0015341966030330213 42-43 0.0 0.0 0.0 0.33905744927029774 0.0015981214614927308 44-45 0.0 0.0 0.0 0.44282880283655907 0.0017259711784121492 46-47 0.0 0.0 0.0 0.5686968491437265 0.0018325126091783312 48-49 0.0 0.0 0.0 0.700424674143034 0.0019177457537912768 50-51 0.0 0.0 0.0 0.848432029763414 0.0019603623260977497 52-53 0.0 0.0 0.0 0.9885979360794033 0.0020882120430171683 54-55 0.0 0.0 0.0 1.1451072978749246 0.0022160617599365864 56-57 0.0 0.0 0.0 1.3312351774234448 0.0022586783322430595 58-59 0.0 0.0 0.0 1.5317674584115526 0.0023226031907027686 60-61 0.0 0.0 0.0 1.7516050466544923 0.0023652197630092413 62-63 0.0 0.0 0.0 1.969610122288254 0.0024717611937754235 64-65 0.0 0.0 0.0 2.1924095623064943 0.0024717611937754235 66-67 0.0 0.0 0.0 2.4581451989235052 0.00249306947992866 68-69 4.2616572306472815E-5 0.0 0.0 2.7533928118627493 0.002514377766081896 70-71 4.2616572306472815E-5 0.0 0.0 3.069884786096769 0.002514377766081896 72-73 4.2616572306472815E-5 0.0 0.0 3.3951770825120766 0.002599610910694842 74-75 4.2616572306472815E-5 0.0 0.0 3.7237508549949823 0.002770077199920733 76-77 4.2616572306472815E-5 0.0 0.0 4.074037771068035 0.002812693772227206 78-79 6.392485845970922E-5 0.0 0.0 4.465193979982995 0.0028553103445336787 80-81 8.523314461294563E-5 0.0 0.0 4.883539562029487 0.0029192352029933878 82-83 8.523314461294563E-5 0.0 0.0 5.313817784321789 0.0030683932060660427 84-85 8.523314461294563E-5 0.0 0.0 5.752065305635403 0.0031536263506789885 86-87 8.523314461294563E-5 0.0 0.0 6.195405507339639 0.0032388594952919344 88-89 8.523314461294563E-5 0.0 0.0 6.663719020415469 0.00332409263990488 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6180 0.0 36.9815 1 GTATCAA 9680 0.0 34.94882 1 ATCAACG 12285 0.0 27.181236 3 TATCAAC 12430 0.0 26.903519 2 TCAACGC 12435 0.0 26.852783 4 GTGTAAG 890 0.0 26.693644 1 CAACGCA 12720 0.0 26.176447 5 AACGCAG 13055 0.0 25.50474 6 TAAGGTA 820 0.0 24.907743 4 TAAGGTG 1450 0.0 24.240606 5 AGGTAAG 1435 0.0 24.164537 2 GTACATG 10570 0.0 23.734869 1 GTAAGGT 1490 0.0 23.589853 4 TACATGG 10575 0.0 23.222967 2 ACGCAGA 14725 0.0 22.612185 7 CGCAGAG 14875 0.0 22.224503 8 AAGGTAA 1460 0.0 21.804686 1 ACATGGG 10855 0.0 21.57278 3 GAGTACT 7310 0.0 21.085169 12-13 AGTACTT 7650 0.0 21.017305 12-13 >>END_MODULE