FastQCFastQC Report
Thu 26 May 2016
SRR938551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4517347
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103890.22998011886180098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77720.1720478856284452No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69970.1548917982169623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55540.12294827030113029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA201750.027.9116331
GTACATG159050.027.2415471
TACATGG157150.027.0561122
ACATGGG161550.024.9649773
ATCAACG231450.024.1653123
TATCAAC234650.024.0181772
TCAACGC233300.023.9533024
CAACGCA234350.023.8256845
AACGCAG241900.023.2009016
GTAAGGT19550.023.111024
GGTATCA144350.023.0966131
TAAGGTG22050.022.2162555
GAGTACT113750.021.36371612-13
ACGCAGA269300.020.6102317
CGCAGAG269500.020.542048
CATGGGG88700.020.4288354
AGAGTAC190900.020.37245810-11
GTACTTT120850.020.0680514-15
GGACCGA12800.020.0481646
AGTACTT122350.019.2407612-13
Produced by FastQC (version 0.11.5)