##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938551_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4517347 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.166612394398747 31.0 31.0 34.0 28.0 34.0 2 31.20835680765724 31.0 31.0 34.0 28.0 34.0 3 31.261218808296107 31.0 31.0 34.0 27.0 34.0 4 34.911423010010076 37.0 35.0 37.0 32.0 37.0 5 34.89103803626332 37.0 35.0 37.0 32.0 37.0 6 34.91159678457289 37.0 35.0 37.0 32.0 37.0 7 34.82258591159811 37.0 35.0 37.0 32.0 37.0 8 34.79236109158761 37.0 35.0 37.0 32.0 37.0 9 36.29245461993511 39.0 35.0 39.0 32.0 39.0 10-11 36.127694529554624 39.0 35.0 39.0 31.0 39.0 12-13 36.00056847525771 38.0 35.0 39.0 30.0 39.0 14-15 36.982552037733655 39.0 36.0 41.0 31.0 41.0 16-17 36.817458344466345 39.0 36.0 41.0 30.5 41.0 18-19 36.95546777787936 39.0 36.0 41.0 31.0 41.0 20-21 36.93697816439605 39.0 36.0 41.0 30.5 41.0 22-23 36.85806149051645 39.0 36.0 41.0 30.0 41.0 24-25 36.72689158038999 39.0 36.0 41.0 30.0 41.0 26-27 36.529174203354316 39.0 36.0 41.0 30.0 41.0 28-29 36.33424662750061 39.0 35.5 40.5 29.5 41.0 30-31 36.107843497521884 39.0 35.0 40.0 29.0 41.0 32-33 35.86111859460874 38.0 35.0 40.0 27.5 41.0 34-35 35.624382187155426 38.0 34.5 40.0 27.0 41.0 36-37 35.36679161463576 38.0 34.0 40.0 26.5 41.0 38-39 35.2614261202427 38.0 34.0 40.0 26.0 41.0 40-41 35.18964460777532 38.0 34.0 40.0 25.5 41.0 42-43 34.929158087700586 38.0 34.0 40.0 25.0 41.0 44-45 34.616111514125436 38.0 33.0 40.0 24.0 41.0 46-47 34.26276230274097 38.0 33.0 40.0 23.5 41.0 48-49 33.93276706438536 38.0 33.0 40.0 22.5 41.0 50-51 33.23660557845125 37.5 32.0 39.5 21.5 40.5 52-53 33.26658202258981 37.0 32.0 39.5 21.0 40.0 54-55 33.39577234159785 37.0 32.5 40.0 20.5 41.0 56-57 33.09173083227833 37.0 32.0 40.0 18.5 41.0 58-59 32.73907018876345 37.0 31.0 40.0 17.5 41.0 60-61 32.271175648007556 36.0 31.0 39.0 13.5 41.0 62-63 31.727586457272377 35.5 30.5 39.0 9.0 40.0 64-65 31.235491650298286 35.0 29.5 39.0 7.5 40.0 66-67 30.713346461983107 35.0 29.0 38.0 4.5 40.0 68-69 30.151006553182654 34.5 28.5 38.0 2.0 40.0 70-71 29.52910901022215 34.0 28.0 37.0 2.0 39.0 72-73 28.90856126394541 34.0 26.5 36.5 2.0 39.0 74-75 28.252438322758913 33.5 26.0 36.0 2.0 39.0 76-77 27.52315097777523 33.0 25.5 35.0 2.0 37.5 78-79 26.75807592376676 32.0 24.0 35.0 2.0 37.0 80-81 26.076232908386274 32.0 21.5 35.0 2.0 36.5 82-83 25.358817575891337 31.0 19.5 34.5 2.0 36.0 84-85 24.6649294375659 31.0 17.5 34.0 2.0 35.5 86-87 23.930528693058115 30.5 9.5 34.0 2.0 35.0 88-89 23.1313716878513 30.0 4.5 34.0 2.0 35.0 90-91 22.23396132730118 29.0 2.0 34.0 2.0 35.0 92-93 21.302066677631807 29.0 2.0 34.0 2.0 35.0 94-95 20.309950508561773 28.0 2.0 34.0 2.0 35.0 96-97 19.068077679221897 25.5 2.0 33.0 2.0 35.0 98-99 17.54381985709754 22.5 2.0 32.5 2.0 34.0 100-101 13.90265259675646 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37127472987500454 1101 2 0.34114502373390465 1101 3 0.25584134883431986 1101 4 0.17223427266597469 1101 5 0.22655267330933526 1101 6 0.4534771736124412 1101 7 0.3739168342747803 1101 8 0.4490329263023938 1101 9 0.5891158919501365 1101 10-11 0.5798263111432007 1101 12-13 0.5782285214421634 1101 14-15 0.7500402384628799 1101 16-17 0.6481915011742956 1101 18-19 0.6328551671301668 1101 20-21 0.6700880611645985 1101 22-23 0.6987400560708252 1101 24-25 0.5629794125602459 1101 26-27 0.6460960403874765 1101 28-29 0.7327421910939762 1101 30-31 0.7061832972003046 1101 32-33 0.7992488768407853 1101 34-35 0.7377362189289727 1101 36-37 0.7547020640353921 1101 38-39 0.7913281646575143 1101 40-41 0.892258822705088 1101 42-43 1.0378604852372462 1101 44-45 0.9266586328436048 1101 46-47 0.8890525461394958 1101 48-49 0.7954392013442231 1101 50-51 0.9408617040288476 1101 52-53 0.960018563278858 1101 54-55 0.9112061951008528 1101 56-57 0.7869936781395239 1101 58-59 0.9394199138720651 1101 60-61 0.8622386409331071 1101 62-63 0.7651763865057966 1101 64-65 0.6735560567723589 1101 66-67 0.8416772985405778 1101 68-69 0.8766988882342943 1101 70-71 0.8581820817568193 1101 72-73 0.9396229797665647 1101 74-75 0.782250313867376 1101 76-77 0.9177152933167712 1101 78-79 1.055939394629437 1101 80-81 0.8792264423389753 1101 82-83 0.911883361372066 1101 84-85 0.820049563932777 1101 86-87 0.9851364634956497 1101 88-89 0.9506816488196641 1101 90-91 0.778415112367913 1101 92-93 0.6860542958756746 1101 94-95 0.5528449930207238 1101 96-97 0.4793814054553458 1101 98-99 0.5580935027443488 1101 100-101 0.6958067093315403 1102 1 0.461126746506217 1102 2 0.32294448503428086 1102 3 0.36353100845822794 1102 4 0.24608800804709574 1102 5 0.3251936385532943 1102 6 0.4005679898377892 1102 7 0.3439216550065467 1102 8 0.477164920806878 1102 9 0.6090527048165626 1102 10-11 0.6921842850887927 1102 12-13 0.5506971851265874 1102 14-15 0.6800313952576431 1102 16-17 0.7304919942858845 1102 18-19 0.5977223226053141 1102 20-21 0.6712268888848669 1102 22-23 0.7338031153583415 1102 24-25 0.6745909214492869 1102 26-27 0.6050424267407521 1102 28-29 0.4033160826842632 1102 30-31 0.6291626902870107 1102 32-33 0.6477767762801889 1102 34-35 0.5675403462985713 1102 36-37 0.8107223971710269 1102 38-39 0.7322079388502019 1102 40-41 0.9319461985618887 1102 42-43 0.937792040824398 1102 44-45 1.0096603147987864 1102 46-47 0.9497826967920417 1102 48-49 1.0799584471647492 1102 50-51 1.0415418450461686 1102 52-53 0.8436690795586443 1102 54-55 0.8238450561858244 1102 56-57 0.8759493380091428 1102 58-59 1.0453297381242734 1102 60-61 0.9616960123807772 1102 62-63 0.9692541144132214 1102 64-65 0.9773745378240157 1102 66-67 0.6860719973571001 1102 68-69 0.8545033473799926 1102 70-71 0.9546187627235341 1102 72-73 0.9353279447460991 1102 74-75 0.964962408914996 1102 76-77 1.0065533348149778 1102 78-79 0.9893858873132153 1102 80-81 1.0838594274944882 1102 82-83 1.1188802512828921 1102 84-85 1.132328398051584 1102 86-87 1.322234987902707 1102 88-89 1.3143039267120855 1102 90-91 1.2834262723931325 1102 92-93 1.3429343781461718 1102 94-95 1.3185380651988616 1102 96-97 1.5633633860215 1102 98-99 1.5175247062871193 1102 100-101 1.3614428744238314 1103 1 0.35100973126162316 1103 2 0.40198562398657955 1103 3 0.32839981800512064 1103 4 0.28662246482078757 1103 5 0.26516666893377305 1103 6 0.32174143269480737 1103 7 0.28408846709763225 1103 8 0.39087312597576584 1103 9 0.4570886525577862 1103 10-11 0.6906850915332967 1103 12-13 0.599309777412337 1103 14-15 0.642008315374099 1103 16-17 0.7463608537765225 1103 18-19 0.7877360718231117 1103 20-21 0.5901882059894277 1103 22-23 0.7798262422477578 1103 24-25 0.783701687850666 1103 26-27 0.7661955689364532 1103 28-29 0.8439257488544314 1103 30-31 0.9087408419573322 1103 32-33 0.8801270699200003 1103 34-35 0.8308465631190387 1103 36-37 0.7871677836915083 1103 38-39 0.6240853827883512 1103 40-41 0.7607914699179759 1103 42-43 0.8965681547901383 1103 44-45 0.8159049274850858 1103 46-47 0.6022898431536348 1103 48-49 0.7550256802521815 1103 50-51 0.92618047270971 1103 52-53 0.8831412329858139 1103 54-55 1.0432451437342607 1103 56-57 0.8072229327538878 1103 58-59 0.8321275217973749 1103 60-61 1.0446122273503846 1103 62-63 0.9301047444388111 1103 64-65 1.0500593076274463 1103 66-67 1.0306712282235075 1103 68-69 1.0711829112830102 1103 70-71 1.1781907096186508 1103 72-73 1.1721715885238417 1103 74-75 1.1787267567231972 1103 76-77 1.1028984813117155 1103 78-79 1.0863208693775341 1103 80-81 1.168826479315527 1103 82-83 1.1417163506180543 1103 84-85 1.3211033956278406 1103 86-87 1.3167776060710672 1103 88-89 1.3228990093102162 1103 90-91 1.3333450109905378 1103 92-93 1.3134089318107236 1103 94-95 1.4246790299356427 1103 96-97 1.5985688457536291 1103 98-99 1.7346494451841856 1103 100-101 1.5924070236322212 1104 1 0.3731546936475496 1104 2 0.28331958074222285 1104 3 0.35730752088872464 1104 4 0.22694658875936824 1104 5 0.2557308382931254 1104 6 0.28112819665988553 1104 7 0.2805561117580453 1104 8 0.42780961761105374 1104 9 0.4880633343758376 1104 10-11 0.48663869110135494 1104 12-13 0.5435396421241236 1104 14-15 0.7456923237569058 1104 16-17 0.7412434750446835 1104 18-19 0.7133733402314633 1104 20-21 0.6806540471781588 1104 22-23 0.6868091009173583 1104 24-25 0.6622093428643439 1104 26-27 0.7133301641433363 1104 28-29 0.6777790831896908 1104 30-31 0.8082672914837801 1104 32-33 0.7370694339708024 1104 34-35 0.6975974474602822 1104 36-37 0.7824938140777746 1104 38-39 0.798285495783638 1104 40-41 0.8114602295024014 1104 42-43 0.9097418196505487 1104 44-45 0.7711650810584914 1104 46-47 0.6735217980000527 1104 48-49 0.4847515414238259 1104 50-51 0.6924748950282265 1104 52-53 0.7877413381883329 1104 54-55 0.950486750493944 1104 56-57 0.9737830053380563 1104 58-59 0.9408142159057533 1104 60-61 0.9582325297658727 1104 62-63 0.9352163064178391 1104 64-65 0.9185488165988822 1104 66-67 0.8914396518834913 1104 68-69 1.0499847158679465 1104 70-71 1.2028392413756137 1104 72-73 1.1538893118078306 1104 74-75 1.078943702120867 1104 76-77 1.0410773923821957 1104 78-79 1.1710740748536317 1104 80-81 1.3306870609351442 1104 82-83 1.238029308617758 1104 84-85 1.3966747037292677 1104 86-87 1.5587472865949614 1104 88-89 1.5862226295013073 1104 90-91 1.5613931425326442 1104 92-93 1.6308362015271491 1104 94-95 1.7766236900730767 1104 96-97 1.9366074589729614 1104 98-99 2.1991824768256514 1104 100-101 1.787997897174904 1105 1 0.3791085062574666 1105 2 0.2572916022578262 1105 3 0.3166571807138219 1105 4 0.2405107005802165 1105 5 0.2648222166396579 1105 6 0.32245537744823594 1105 7 0.4134201019912922 1105 8 0.40811325243132757 1105 9 0.5105803917581895 1105 10-11 0.49853755640750563 1105 12-13 0.5367208207252716 1105 14-15 0.5962758359885925 1105 16-17 0.599633587000568 1105 18-19 0.48250204993069445 1105 20-21 0.6240407908818568 1105 22-23 0.653608452819725 1105 24-25 0.6287100209339656 1105 26-27 0.5717676057820427 1105 28-29 0.5623284154410868 1105 30-31 0.5872896307279944 1105 32-33 0.6313660398363439 1105 34-35 0.5747308982862833 1105 36-37 0.6631985901866742 1105 38-39 0.7575602648840842 1105 40-41 0.8630134981713553 1105 42-43 0.9158337251085413 1105 44-45 0.9084912928696482 1105 46-47 0.8158792335429013 1105 48-49 0.906450569303523 1105 50-51 0.8935241967114465 1105 52-53 0.888196864158914 1105 54-55 0.9817589362827661 1105 56-57 0.8972177774257588 1105 58-59 0.9247733234781776 1105 60-61 0.9791624999826922 1105 62-63 0.9713726278356525 1105 64-65 1.078880810528343 1105 66-67 1.2303740740191849 1105 68-69 1.0840366445316008 1105 70-71 1.2787618054122838 1105 72-73 1.3075620978102513 1105 74-75 1.3003713609494767 1105 76-77 1.296495372839793 1105 78-79 1.3154892794907092 1105 80-81 1.3802296287027502 1105 82-83 1.414192244685605 1105 84-85 1.3985448799259146 1105 86-87 1.5251693346334925 1105 88-89 1.3966323670108878 1105 90-91 1.3210149663383035 1105 92-93 1.4022082858944778 1105 94-95 1.5224915341976555 1105 96-97 1.830376456545448 1105 98-99 1.8634025649760098 1105 100-101 1.6365898495703437 1106 1 0.20145791099001187 1106 2 0.13702907247607143 1106 3 0.12761029998717532 1106 4 0.14016067721026104 1106 5 0.23441426826207845 1106 6 0.06709491699737669 1106 7 0.24260127339725557 1106 8 0.25594325765267456 1106 9 0.12609300555842395 1106 10-11 0.08457513071574141 1106 12-13 0.18769824331926088 1106 14-15 0.34959085120075883 1106 16-17 0.2132917437776598 1106 18-19 0.20083556044063755 1106 20-21 0.19867753543168476 1106 22-23 0.15400995952553131 1106 24-25 0.04287967177553753 1106 26-27 0.10801382980129404 1106 28-29 0.36268859455387314 1106 30-31 0.10118558710843928 1106 32-33 0.2192098964901561 1106 34-35 -0.12926897345214883 1106 36-37 0.037680144365602075 1106 38-39 0.17241733033495876 1106 40-41 0.3784160684474074 1106 42-43 -0.12523369401237971 1106 44-45 0.13035449843464875 1106 46-47 0.11972070326525852 1106 48-49 0.40165702294365246 1106 50-51 0.06377197539161727 1106 52-53 0.03578400977633578 1106 54-55 0.18995365572538958 1106 56-57 -0.593412898799663 1106 58-59 -0.30198554753000195 1106 60-61 0.41143763452713245 1106 62-63 0.32916789827200077 1106 64-65 0.49851092193311075 1106 66-67 -0.011812590678626833 1106 68-69 0.045010943302834505 1106 70-71 -0.0232090980015478 1106 72-73 0.2922318023154098 1106 74-75 0.6288520209737065 1106 76-77 0.5898598586206809 1106 78-79 0.8348462836190755 1106 80-81 0.7578821335970645 1106 82-83 0.4317909113780054 1106 84-85 0.3955454126811695 1106 86-87 0.41374572210760974 1106 88-89 0.46582774726610054 1106 90-91 0.6261246274265098 1106 92-93 0.41957687829218315 1106 94-95 0.34058019888750124 1106 96-97 0.6565380432472558 1106 98-99 0.7596727318355256 1106 100-101 0.33026867320752196 1107 1 0.22205775898897429 1107 2 0.09057678525969948 1107 3 0.15702525759276398 1107 4 0.08814899242752716 1107 5 0.17049555031811536 1107 6 0.22859811125308482 1107 7 0.21887841720364065 1107 8 0.17295877097938472 1107 9 0.3454406273695696 1107 10-11 0.41372900586290484 1107 12-13 0.3916060436151909 1107 14-15 0.5716703565842565 1107 16-17 0.5761409647366946 1107 18-19 0.48469355401742575 1107 20-21 0.5878739119322702 1107 22-23 0.612747262471963 1107 24-25 0.5787002278483797 1107 26-27 0.5219103987509683 1107 28-29 0.6923653238033296 1107 30-31 0.7146944773630253 1107 32-33 0.6206908847155361 1107 34-35 0.7393546946969849 1107 36-37 0.7147822669141775 1107 38-39 0.7559263821916531 1107 40-41 0.7641944178530267 1107 42-43 0.8958748441397333 1107 44-45 0.8115929694315867 1107 46-47 0.7992852854073789 1107 48-49 0.776456101621541 1107 50-51 0.7865223671578931 1107 52-53 0.9259351695328419 1107 54-55 0.9491785930213936 1107 56-57 0.9268377460640309 1107 58-59 0.9184109907628084 1107 60-61 0.8225543858685498 1107 62-63 0.9502949256060482 1107 64-65 0.9811543093413597 1107 66-67 0.9714245894026767 1107 68-69 1.149423037224075 1107 70-71 1.2319262841612897 1107 72-73 1.2555116928822692 1107 74-75 1.1563436521235566 1107 76-77 1.2944526854835168 1107 78-79 1.2813406111475665 1107 80-81 1.2851967866777443 1107 82-83 1.0431410589349888 1107 84-85 1.2019443569441286 1107 86-87 1.3553787969251054 1107 88-89 1.3010449333647074 1107 90-91 1.2366394109295982 1107 92-93 1.377743488160462 1107 94-95 1.339300381516889 1107 96-97 1.570737901322584 1107 98-99 1.7253291714654964 1107 100-101 1.5504271335767434 1108 1 0.31984721130850247 1108 2 0.22177803390971462 1108 3 0.17697967317844387 1108 4 0.224905086395772 1108 5 0.24615427406764923 1108 6 0.2551893062159465 1108 7 0.2950459323462127 1108 8 0.3075028917642797 1108 9 0.3018484777723245 1108 10-11 0.39494581059593514 1108 12-13 0.4254794766085226 1108 14-15 0.46938534334857707 1108 16-17 0.6040477404686655 1108 18-19 0.502759834470865 1108 20-21 0.43603712884425505 1108 22-23 0.434441447755205 1108 24-25 0.5061465887771135 1108 26-27 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-1.6720750139544087 2316 66-67 -1.6658139290381762 2316 68-69 -1.7458453204484456 2316 70-71 -1.8722315027131344 2316 72-73 -1.8319108634298793 2316 74-75 -1.741595147190953 2316 76-77 -1.848026995237138 2316 78-79 -2.1899695307602336 2316 80-81 -2.3083521367637907 2316 82-83 -2.356737624927714 2316 84-85 -2.3848623272972738 2316 86-87 -2.5108457889383367 2316 88-89 -2.7281675699826344 2316 90-91 -2.736126407260901 2316 92-93 -2.8555531164938763 2316 94-95 -3.0653671991611766 2316 96-97 -3.2699635649360985 2316 98-99 -3.220398718511994 2316 100-101 -2.663317914359954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13982.0 3 4837.0 4 5157.0 5 5917.0 6 7175.0 7 8698.0 8 11268.0 9 14233.0 10 18167.0 11 22301.0 12 27121.0 13 31264.0 14 35962.0 15 40728.0 16 46710.0 17 53893.0 18 53243.0 19 52266.0 20 55856.0 21 61200.0 22 67623.0 23 75419.0 24 85154.0 25 94881.0 26 107008.0 27 121445.0 28 138562.0 29 158696.0 30 183875.0 31 215265.0 32 253738.0 33 302311.0 34 368918.0 35 452162.0 36 536923.0 37 529432.0 38 244125.0 39 11832.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7526566540328 18.173284687046156 14.082878750309943 23.9911799086111 2 15.498551975214832 19.79493868303647 39.92415625327548 24.782353088473215 3 19.473121184432408 23.446020861778912 29.17719259326401 27.903665360524666 4 12.929292257707775 15.89458718819015 36.335639321700334 34.84048123240174 5 14.129469363357527 37.057830512172316 33.8002553256777 15.012444798792457 6 33.33783142098134 35.28838392130872 17.033438074517136 14.340346583192796 7 29.738519180995016 31.951260395162883 20.07006833012892 18.24015209371318 8 26.80214202614735 34.78294757996837 19.3649030645482 19.050007329336076 9 26.567032848294346 16.921120607005054 19.753725252627238 36.75812129207336 10-11 25.34959482481389 26.117565033120094 27.07352731422051 21.459312827845505 12-13 26.220345400508954 23.32885895040301 27.43222727369879 23.018568375389247 14-15 22.839476649023236 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27.896848019520192 22.980992765955516 40-41 22.909441442215513 26.433520331978137 27.95321188054438 22.703826345261962 42-43 22.690804716565623 26.351050164163926 27.714196442668403 23.243948676602052 44-45 22.639480833502784 26.526565861772045 27.90142460404389 22.93252870068128 46-47 22.98910772333761 26.92128657165329 27.274738984431778 22.81486672057732 48-49 22.904173173199656 26.674230055804436 27.45465979194518 22.966936979050725 50-51 22.86876571853636 26.924767985547803 27.435266196734087 22.77120009918175 52-53 23.084001609508277 26.813931877064118 27.229902317016673 22.872164196410928 54-55 22.596094506283983 26.733165922876434 27.499685897836816 23.171053673002767 56-57 22.7851839847039 26.958534171023267 27.34381486335067 22.912466980922165 58-59 22.93354742317254 26.473447044205184 27.382716289928183 23.210289242694095 60-61 22.55260796822841 26.81087746521661 27.749568445935953 22.88694612061903 62-63 22.46868844886235 26.819677589792917 27.814591382638703 22.89704257870603 64-65 22.26914318429271 26.843595677953232 27.62758986516075 23.259671272593312 66-67 22.369096006335347 26.78240882697987 27.43786140828656 23.410633758398223 68-69 22.56340506213233 26.536814415970948 27.66680507428681 23.23297544760991 70-71 22.655426193345352 26.52478896156379 27.45544621105128 23.364338634039576 72-73 22.598680590446072 26.51655373395384 27.59187052754018 23.292895148059912 74-75 22.430640991233965 27.0777477221335 27.21062880364069 23.280982482991842 76-77 22.621238485220974 26.557721664927968 27.279117236162975 23.541922613688083 78-79 22.74848146908008 26.683827334386734 27.393749878932617 23.17394131760057 80-81 22.83432745765586 26.864014928080906 27.14259923542068 23.15905837884256 82-83 22.8090940698246 26.777048557191485 27.220532399779458 23.193324973204454 84-85 22.774641065069424 26.723401269040366 27.22636621524274 23.27559145064747 86-87 22.779460280188125 26.753799357104253 27.09482988989209 23.37191047281553 88-89 22.724351606904087 26.74450174081461 26.954513384629898 23.57663326765141 90-91 22.757319134353857 26.648441838050363 27.015950046696975 23.578288980898808 92-93 22.88733692546719 26.305971905006377 27.24579316337219 23.560898006154243 94-95 22.687681951416135 26.221655997351217 27.382800864175792 23.707861187056853 96-97 22.68362311055716 26.098608300853193 27.39069393171425 23.827074656875396 98-99 22.80656129587387 26.211327913783183 26.952718654137662 24.02939213620528 100-101 23.247583815735755 25.534238849066217 27.30190191090507 23.91627542429297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 704.0 1 495.0 2 403.5 3 516.5 4 458.0 5 479.5 6 824.5 7 1701.5 8 2734.0 9 3157.5 10 2772.5 11 2371.0 12 2400.0 13 2645.0 14 2975.0 15 3245.5 16 3488.5 17 3759.0 18 4133.0 19 4652.0 20 5180.5 21 6021.0 22 7263.5 23 8938.5 24 11159.5 25 14331.5 26 18891.0 27 24186.5 28 29544.0 29 35801.5 30 43305.5 31 52212.0 32 64482.5 33 72155.5 34 75903.0 35 83687.5 36 94594.0 37 104918.5 38 112862.5 39 119747.5 40 126453.0 41 137170.0 42 146743.5 43 153904.5 44 160672.5 45 168968.5 46 176642.0 47 184905.5 48 193115.0 49 191124.5 50 181390.5 51 169381.5 52 161947.5 53 161301.0 54 163000.5 55 154771.5 56 136672.5 57 121986.5 58 111116.0 59 96987.0 60 79565.5 61 66421.0 62 56515.5 63 44387.0 64 33971.0 65 26835.5 66 20256.5 67 15891.0 68 12332.5 69 8642.0 70 6427.5 71 4871.0 72 3795.0 73 3048.5 74 2248.5 75 1753.0 76 1277.0 77 774.0 78 471.0 79 310.0 80 197.5 81 127.5 82 71.5 83 43.0 84 28.5 85 18.0 86 13.5 87 11.0 88 6.5 89 4.5 90 6.0 91 6.0 92 4.5 93 2.5 94 1.0 95 2.0 96 2.0 97 1.0 98 0.5 99 0.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008566975262250165 2 0.10289225069493221 3 0.11236683832346729 4 0.1147133483436185 5 0.15560017860040418 6 0.0955870779906879 7 0.026210074187349344 8 0.02764897184121565 9 0.022336118965401594 10-11 0.05009577524153004 12-13 0.05280754389689347 14-15 0.026984865231738896 16-17 0.030072960965805817 18-19 0.035053760536881494 20-21 0.05738987950228309 22-23 0.03010616629627965 24-25 0.04702981639444568 26-27 0.05550824410876561 28-29 0.05183352086966089 30-31 0.06651027693909721 32-33 0.04811452385659105 34-35 0.060566522784280236 36-37 0.05181138398267832 38-39 0.031179805314933744 40-41 0.03603885200760534 42-43 0.03174429593298898 44-45 0.007471199356613516 46-47 0.013603117050782241 48-49 0.009131465880305408 50-51 0.008566975262250165 52-53 0.00972916182883449 54-55 0.011168059482700797 56-57 0.014167607668837484 58-59 0.019104133465948043 60-61 0.024594081437622567 62-63 0.011588660335369412 64-65 0.009142534323796688 66-67 0.01036006310783741 68-69 0.01096882749985777 70-71 0.029774112991541275 72-73 0.02736119231044239 74-75 0.02831307845069241 76-77 0.022834198922509163 78-79 0.005191099997409985 80-81 0.004925457353619282 82-83 0.006010164815764651 84-85 0.005157894666936147 86-87 0.013193584641604906 88-89 0.03641517908630885 90-91 0.033858368639823326 92-93 0.028235599346253453 94-95 0.04564626095803577 96-97 0.04196046927543976 98-99 0.024461260115727218 100-101 0.07322882213830374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4517347.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.42583193929862 #Duplication Level Percentage of deduplicated Percentage of total 1 72.73183244599302 33.76635829774898 2 13.6431049262172 12.667849928695537 3 5.377980116527909 7.490316031884428 4 2.6184387752145053 4.862527940858062 5 1.4753668343566149 3.424756635032761 6 0.9123085997167419 2.541281143633579 7 0.609913271602764 1.9821011731484397 8 0.4392967373432161 1.6315773199502688 9 0.31960041416315205 1.3353943604101857 >10 1.6558902867290919 13.86106407877449 >50 0.1266563872636673 4.0442286775337095 >100 0.08088870530074038 7.162913755329339 >500 0.005128063276003391 1.6256267363218138 >1k 0.0034027330261826422 2.920663168157247 >5k 1.437774518105275E-4 0.45218592450793177 >10k+ 4.792581727017583E-5 0.23115482801323145 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10389 0.22998011886180098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7772 0.1720478856284452 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6997 0.1548917982169623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5554 0.12294827030113029 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.2136886982558568E-5 0.0 4 0.0 0.0 0.0 2.2136886982558568E-5 0.0 5 0.0 0.0 0.0 2.2136886982558568E-5 0.0 6 0.0 0.0 0.0 2.2136886982558568E-5 4.4273773965117135E-5 7 0.0 0.0 0.0 2.2136886982558568E-5 4.4273773965117135E-5 8 0.0 0.0 0.0 2.2136886982558568E-5 4.4273773965117135E-5 9 0.0 0.0 0.0 4.4273773965117135E-5 4.4273773965117135E-5 10-11 0.0 0.0 0.0 4.4273773965117135E-5 4.4273773965117135E-5 12-13 0.0 0.0 0.0 4.4273773965117135E-5 6.641066094767571E-5 14-15 0.0 0.0 0.0 4.4273773965117135E-5 6.641066094767571E-5 16-17 0.0 0.0 0.0 6.641066094767571E-5 6.641066094767571E-5 18-19 0.0 0.0 0.0 8.854754793023427E-5 6.641066094767571E-5 20-21 0.0 0.0 0.0 1.6602665236918927E-4 6.641066094767571E-5 22-23 0.0 0.0 0.0 2.877795307732614E-4 6.641066094767571E-5 24-25 0.0 2.2136886982558568E-5 0.0 4.759430701250092E-4 6.641066094767571E-5 26-27 0.0 2.2136886982558568E-5 0.0 9.850914707238564E-4 6.641066094767571E-5 28-29 0.0 2.2136886982558568E-5 0.0 0.0032873277169099477 6.641066094767571E-5 30-31 0.0 2.2136886982558568E-5 0.0 0.011145922595718238 6.641066094767571E-5 32-33 0.0 2.2136886982558568E-5 0.0 0.029740907661067434 6.641066094767571E-5 34-35 0.0 2.2136886982558568E-5 0.0 0.05841924474697206 6.641066094767571E-5 36-37 0.0 2.2136886982558568E-5 0.0 0.10699864323019684 6.641066094767571E-5 38-39 2.2136886982558568E-5 2.2136886982558568E-5 0.0 0.18162208924840179 6.641066094767571E-5 40-41 2.2136886982558568E-5 2.2136886982558568E-5 0.0 0.26278698536995276 6.641066094767571E-5 42-43 2.2136886982558568E-5 2.2136886982558568E-5 0.0 0.35121278042178294 6.641066094767571E-5 44-45 2.2136886982558568E-5 2.2136886982558568E-5 0.0 0.45925185734016005 7.7479104438955E-5 46-47 2.2136886982558568E-5 2.2136886982558568E-5 0.0 0.5881992240135637 8.854754793023427E-5 48-49 2.2136886982558568E-5 2.2136886982558568E-5 0.0 0.7244628318347031 8.854754793023427E-5 50-51 2.2136886982558568E-5 2.2136886982558568E-5 0.0 0.8734662181143047 1.1068443491279284E-4 52-53 2.2136886982558568E-5 2.2136886982558568E-5 0.0 1.018484964737046 1.3282132189535142E-4 54-55 2.2136886982558568E-5 2.2136886982558568E-5 0.0 1.178556794507927 1.3282132189535142E-4 56-57 2.2136886982558568E-5 2.2136886982558568E-5 0.0 1.3701183460114974 1.3282132189535142E-4 58-59 2.2136886982558568E-5 2.2136886982558568E-5 0.0 1.5752055354614112 1.438897653866307E-4 60-61 2.2136886982558568E-5 2.2136886982558568E-5 0.0 1.796065256886398 1.5495820887790997E-4 62-63 2.2136886982558568E-5 2.2136886982558568E-5 0.0 2.0188951612528325 2.213688698255857E-4 64-65 2.2136886982558568E-5 2.2136886982558568E-5 0.0 2.256567848341073 2.213688698255857E-4 66-67 2.2136886982558568E-5 2.2136886982558568E-5 0.0 2.531186999803203 2.213688698255857E-4 68-69 2.2136886982558568E-5 2.2136886982558568E-5 0.0 2.835513853595927 2.3243731331686496E-4 70-71 2.2136886982558568E-5 4.4273773965117135E-5 0.0 3.1540525888314535 2.4350575680814424E-4 72-73 2.2136886982558568E-5 4.4273773965117135E-5 0.0 3.477538918307582 2.656426437907028E-4 74-75 2.2136886982558568E-5 4.4273773965117135E-5 0.0 3.8079098196352863 2.877795307732614E-4 76-77 2.2136886982558568E-5 4.4273773965117135E-5 0.0 4.167412864232038 3.0991641775581993E-4 78-79 2.2136886982558568E-5 4.4273773965117135E-5 0.0 4.5710125876980445 3.0991641775581993E-4 80-81 2.2136886982558568E-5 4.4273773965117135E-5 0.0 5.000767043133946 3.0991641775581993E-4 82-83 2.2136886982558568E-5 4.4273773965117135E-5 0.0 5.434517206670199 3.0991641775581993E-4 84-85 2.2136886982558568E-5 6.641066094767571E-5 0.0 5.870060458052038 3.0991641775581993E-4 86-87 2.2136886982558568E-5 6.641066094767571E-5 0.0 6.324641432238878 3.431217482296578E-4 88-89 2.2136886982558568E-5 6.641066094767571E-5 0.0 6.817430673357615 3.763270787034957E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 20175 0.0 27.911633 1 GTACATG 15905 0.0 27.241547 1 TACATGG 15715 0.0 27.056112 2 ACATGGG 16155 0.0 24.964977 3 ATCAACG 23145 0.0 24.165312 3 TATCAAC 23465 0.0 24.018177 2 TCAACGC 23330 0.0 23.953302 4 CAACGCA 23435 0.0 23.825684 5 AACGCAG 24190 0.0 23.200901 6 GTAAGGT 1955 0.0 23.11102 4 GGTATCA 14435 0.0 23.096613 1 TAAGGTG 2205 0.0 22.216255 5 GAGTACT 11375 0.0 21.363716 12-13 ACGCAGA 26930 0.0 20.610231 7 CGCAGAG 26950 0.0 20.54204 8 CATGGGG 8870 0.0 20.428835 4 AGAGTAC 19090 0.0 20.372458 10-11 GTACTTT 12085 0.0 20.06805 14-15 GGACCGA 1280 0.0 20.048164 6 AGTACTT 12235 0.0 19.24076 12-13 >>END_MODULE