FastQCFastQC Report
Thu 26 May 2016
SRR938551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4517347
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104010.23024576150559167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83250.1842895841298001No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69070.15289947838853202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57470.12722068948876408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA197700.025.5824991
GGTATCA145550.025.277651
GTACATG157250.024.8176381
TACATGG156500.024.5414492
ACATGGG158950.023.3428083
ATCAACG225400.022.2573073
TCAACGC228300.022.057824
CAACGCA229500.021.9424845
TATCAAC230950.021.7344722
AACGCAG235550.021.3872266
GAGTACT115550.020.91558612-13
GTACTTT117100.020.3346314-15
CATGGGG84300.019.949794
TAAGGTG21100.019.8135745
GTAAGGT21700.019.0468044
AGAGTAC192000.019.02959610-11
ACGCAGA267150.018.8218787
CGCAGAG267100.018.7542978
AGTACTT122050.018.71239912-13
GAGTACA124600.018.1210581
Produced by FastQC (version 0.11.5)