##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938551_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4517347 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.815160535597553 31.0 31.0 34.0 30.0 34.0 2 32.0387827191491 31.0 31.0 34.0 30.0 34.0 3 32.10765832246228 33.0 31.0 34.0 30.0 34.0 4 35.77295191181904 37.0 35.0 37.0 35.0 37.0 5 35.66842064601192 37.0 35.0 37.0 33.0 37.0 6 35.66542751752301 37.0 35.0 37.0 33.0 37.0 7 35.60371275441094 37.0 35.0 37.0 33.0 37.0 8 35.58165002599977 37.0 35.0 37.0 33.0 37.0 9 37.16750982379702 39.0 37.0 39.0 33.0 39.0 10-11 37.069610658645445 39.0 37.0 39.0 33.0 39.0 12-13 36.96146388577189 39.0 37.0 39.0 33.0 39.0 14-15 38.02538868499586 40.0 37.0 41.0 33.0 41.0 16-17 37.93847129742302 40.0 37.0 41.0 33.0 41.0 18-19 38.1698047548705 40.0 37.5 41.0 33.0 41.0 20-21 38.13502405283455 40.0 37.0 41.0 33.0 41.0 22-23 38.028258511024276 40.0 37.0 41.0 33.0 41.0 24-25 37.910583025833525 40.0 37.0 41.0 32.5 41.0 26-27 37.72554687519024 40.0 37.0 41.0 32.0 41.0 28-29 37.62193074829098 40.0 37.0 41.0 32.0 41.0 30-31 37.397249093328455 39.0 36.0 41.0 31.5 41.0 32-33 37.21885522630872 39.0 36.0 41.0 31.0 41.0 34-35 37.13809056510381 39.0 36.0 41.0 31.0 41.0 36-37 37.109670565489 39.0 36.0 41.0 31.0 41.0 38-39 36.9638892031097 39.0 36.0 41.0 31.0 41.0 40-41 36.816455322117164 39.0 35.5 41.0 30.0 41.0 42-43 36.6199798244412 39.0 35.0 40.5 30.0 41.0 44-45 36.349704372942796 39.0 35.0 40.0 30.0 41.0 46-47 36.00801189282116 38.5 35.0 40.0 29.5 41.0 48-49 35.68573855406724 38.0 35.0 40.0 28.0 41.0 50-51 35.33333469844136 38.0 34.0 40.0 27.0 41.0 52-53 34.86185641705187 38.0 34.0 40.0 26.0 41.0 54-55 34.53921261749429 38.0 33.0 40.0 26.0 41.0 56-57 34.20862444262086 37.0 33.0 40.0 25.0 41.0 58-59 33.75420528907786 37.0 32.5 39.5 23.5 41.0 60-61 33.355129017097866 36.0 32.0 39.0 23.0 40.5 62-63 32.891104446924274 36.0 31.0 39.0 22.0 40.0 64-65 32.384241569221935 35.0 31.0 39.0 20.5 40.0 66-67 31.883082813872832 35.0 30.5 38.0 19.5 40.0 68-69 31.39406691582471 35.0 30.0 38.0 18.0 40.0 70-71 30.820668967869857 34.0 29.0 37.0 14.5 39.0 72-73 30.320916679635193 34.0 29.0 36.5 11.5 39.0 74-75 29.733748370448406 34.0 28.5 36.0 8.0 39.0 76-77 28.488973173856248 31.5 27.0 34.5 7.0 36.5 78-79 29.193485246982355 33.5 28.5 35.0 7.0 37.0 80-81 28.818345369527734 33.0 28.0 35.0 2.0 37.0 82-83 28.38050353448606 33.0 27.0 35.0 2.0 36.0 84-85 27.82646451556633 33.0 26.0 35.0 2.0 36.0 86-87 27.225576317249924 32.5 25.5 35.0 2.0 35.5 88-89 26.486276015546295 32.0 24.5 34.0 2.0 35.0 90-91 25.887931124175317 31.5 23.5 34.0 2.0 35.0 92-93 25.058112095440087 31.0 20.0 34.0 2.0 35.0 94-95 24.254532361583024 31.0 16.5 34.0 2.0 35.0 96-97 23.186436640798238 30.5 2.0 34.0 2.0 35.0 98-99 21.826911016576766 30.0 2.0 34.0 2.0 35.0 100-101 17.82391921630107 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21572911648913617 1101 2 0.27389715911585455 1101 3 0.2749467747004388 1101 4 0.19048687641437567 1101 5 0.0371588261501401 1101 6 0.14132291941712083 1101 7 0.17615027774862568 1101 8 0.20022696998364609 1101 9 0.27664764527401076 1101 10-11 0.19400983490976387 1101 12-13 0.2907591165197161 1101 14-15 0.3428503514737429 1101 16-17 0.29329989198187434 1101 18-19 0.38776787949325353 1101 20-21 0.29872773745655223 1101 22-23 0.24188582554379678 1101 24-25 0.3518898355135889 1101 26-27 0.289957869919121 1101 28-29 0.27646432624865724 1101 30-31 0.36805174312168276 1101 32-33 0.3050053033277891 1101 34-35 0.29789857917357665 1101 36-37 0.37684524860833335 1101 38-39 0.4072331671159546 1101 40-41 0.5806939819835506 1101 42-43 0.5630758126034365 1101 44-45 0.48002690416079474 1101 46-47 0.39510667642163355 1101 48-49 0.4479422622813942 1101 50-51 0.5543366694873484 1101 52-53 0.6319848881632097 1101 54-55 0.667302416182217 1101 56-57 0.611949133659202 1101 58-59 0.6244708925394491 1101 60-61 0.5182533722809666 1101 62-63 0.5507898754028773 1101 64-65 0.580702280100553 1101 66-67 0.4063152965091952 1101 68-69 0.574740840807376 1101 70-71 0.5089000653385263 1101 72-73 0.4056730640142696 1101 74-75 0.5166047082068879 1101 76-77 0.512792329711484 1101 78-79 0.6530123476310017 1101 80-81 0.6727989508104635 1101 82-83 0.5717253732639662 1101 84-85 0.5890703691553227 1101 86-87 0.7316548170258166 1101 88-89 0.9027638385712287 1101 90-91 0.9719569248100797 1101 92-93 1.0261260500299692 1101 94-95 0.9111302398078998 1101 96-97 0.961739640069645 1101 98-99 1.0918947344005225 1101 100-101 1.3748384280542432 1102 1 0.30503806802051514 1102 2 0.25000871820786585 1102 3 0.22183440924281683 1102 4 0.1848861589797295 1102 5 0.10699981683858084 1102 6 0.12835764552409046 1102 7 0.127864880449863 1102 8 0.22425011697979613 1102 9 0.2574015129290217 1102 10-11 0.3361429993615772 1102 12-13 0.3497218422225714 1102 14-15 0.5057768973664238 1102 16-17 0.4561319877030243 1102 18-19 0.3995327101034647 1102 20-21 0.398025363698288 1102 22-23 0.376765378968166 1102 24-25 0.40620941074039507 1102 26-27 0.3974275095990478 1102 28-29 0.3016560797415053 1102 30-31 0.37548412035360457 1102 32-33 0.30674765797773773 1102 34-35 0.29443672625391315 1102 36-37 0.4257319277353133 1102 38-39 0.4156820452200378 1102 40-41 0.25030943352023627 1102 42-43 0.3242921836339008 1102 44-45 0.41338646152656366 1102 46-47 0.5590657507164067 1102 48-49 0.5290325521551793 1102 50-51 0.7155062675705253 1102 52-53 0.6684135304193859 1102 54-55 0.6086332998603936 1102 56-57 0.592081700664572 1102 58-59 0.6644512229560249 1102 60-61 0.6083949134558182 1102 62-63 0.6384289703525852 1102 64-65 0.6361705989124502 1102 66-67 0.5560079982455939 1102 68-69 0.7414250317382916 1102 70-71 0.6613907247809543 1102 72-73 0.7623589450076729 1102 74-75 0.7474574850544045 1102 76-77 0.6396537838761738 1102 78-79 0.8583055485282891 1102 80-81 0.9123638439093327 1102 82-83 0.9187678574437967 1102 84-85 0.8546181021952997 1102 86-87 0.661657854149194 1102 88-89 0.7245614663720694 1102 90-91 0.8610033241974158 1102 92-93 1.20627395856204 1102 94-95 1.158115754537949 1102 96-97 1.0187872318553204 1102 98-99 1.0473579393701797 1102 100-101 1.1758436410551276 1103 1 0.20946713762739932 1103 2 0.2124224110186077 1103 3 0.2023531778064651 1103 4 0.1797211536768728 1103 5 0.09194883015592836 1103 6 0.08969995697913191 1103 7 0.17036283128759067 1103 8 0.1560480034782401 1103 9 0.2746028420200801 1103 10-11 0.23404218939163002 1103 12-13 0.30212601634406866 1103 14-15 0.3900083954142062 1103 16-17 0.3688494559667177 1103 18-19 0.3617348471079538 1103 20-21 0.3452232848635717 1103 22-23 0.3007558837818678 1103 24-25 0.41989525874102895 1103 26-27 0.38423266731843597 1103 28-29 0.4443501919621582 1103 30-31 0.435334582920774 1103 32-33 0.4448235019534934 1103 34-35 0.49035074076110874 1103 36-37 0.49560160395298425 1103 38-39 0.5039949456880493 1103 40-41 0.6396598711155335 1103 42-43 0.6826955704070343 1103 44-45 0.6385644815804454 1103 46-47 0.5946740507783943 1103 48-49 0.5419418925158865 1103 50-51 0.6897950246471183 1103 52-53 0.5843529280978856 1103 54-55 0.5554813210600429 1103 56-57 0.529677232756363 1103 58-59 0.649692289636782 1103 60-61 0.6732113114545442 1103 62-63 0.7465514382784235 1103 64-65 0.6925021137060767 1103 66-67 0.6526315076452995 1103 68-69 0.714700782877447 1103 70-71 0.5773923464835669 1103 72-73 0.6695278694251705 1103 74-75 0.6539655069003842 1103 76-77 0.5905102602571937 1103 78-79 0.7987403234729875 1103 80-81 0.7473852756494637 1103 82-83 0.7667939339517318 1103 84-85 0.6748695248574172 1103 86-87 0.7845630227386913 1103 88-89 0.8833878178643602 1103 90-91 0.9423436379028587 1103 92-93 1.265696197628035 1103 94-95 1.0264302120754856 1103 96-97 0.9434787705044307 1103 98-99 1.2181006321603611 1103 100-101 1.3425428005313655 1104 1 0.15285504824422702 1104 2 0.24730194300657615 1104 3 0.21019416058720708 1104 4 0.15907952966175998 1104 5 0.13773112774545382 1104 6 0.1548146917324189 1104 7 0.15270712182465473 1104 8 0.17659411028940752 1104 9 0.29122606394450656 1104 10-11 0.261205645711712 1104 12-13 0.2580021612563641 1104 14-15 0.35477743693024166 1104 16-17 0.4114111372030962 1104 18-19 0.3859516032524226 1104 20-21 0.3657474327371162 1104 22-23 0.32515245829892336 1104 24-25 0.30925318942426117 1104 26-27 0.32783298682236506 1104 28-29 0.3690264437468329 1104 30-31 0.4077416888218579 1104 32-33 0.4770955422978176 1104 34-35 0.505110542267893 1104 36-37 0.41068244011815835 1104 38-39 0.42629234039507935 1104 40-41 0.45778245152727237 1104 42-43 0.5186542909440703 1104 44-45 0.524261587398037 1104 46-47 0.4868808822623407 1104 48-49 0.399367492351125 1104 50-51 0.5164124576500839 1104 52-53 0.4972204759051948 1104 54-55 0.5671102901588867 1104 56-57 0.463298564931911 1104 58-59 0.5637685809419892 1104 60-61 0.46978193212154196 1104 62-63 0.4690326312415394 1104 64-65 0.5147393370276419 1104 66-67 0.4848418458797319 1104 68-69 0.607881043768554 1104 70-71 0.617942572335 1104 72-73 0.5607935002088702 1104 74-75 0.6845989763290348 1104 76-77 0.6999514345176436 1104 78-79 0.9426554434122032 1104 80-81 0.7757874498347697 1104 82-83 0.9517621087994215 1104 84-85 1.078346050502617 1104 86-87 1.1870389834074224 1104 88-89 1.118478116689598 1104 90-91 1.0729800017922386 1104 92-93 1.362778135914791 1104 94-95 1.192646233472228 1104 96-97 1.286366273391934 1104 98-99 1.6282822039081957 1104 100-101 2.075767480262069 1105 1 0.20209758243129272 1105 2 0.20911530894038322 1105 3 0.2010857853932535 1105 4 0.1396278667869595 1105 5 0.06986193150859066 1105 6 0.1335015341276602 1105 7 0.20682147546357044 1105 8 0.13183236346743854 1105 9 0.2161084706018741 1105 10-11 0.22835212553648176 1105 12-13 0.2281846179681395 1105 14-15 0.346402802565116 1105 16-17 0.28250406534831995 1105 18-19 0.3116620178693381 1105 20-21 0.3108730062303593 1105 22-23 0.27023655948445224 1105 24-25 0.3618983355717802 1105 26-27 0.2421613710398205 1105 28-29 0.28310557573405504 1105 30-31 0.3307677259524908 1105 32-33 0.3268908976399274 1105 34-35 0.3863942310000752 1105 36-37 0.41506624085695165 1105 38-39 0.40470422609698176 1105 40-41 0.42128920029550443 1105 42-43 0.4117636016445019 1105 44-45 0.43566797820221126 1105 46-47 0.5239797180933436 1105 48-49 0.6363142926893701 1105 50-51 0.5850283646178198 1105 52-53 0.5916388524154854 1105 54-55 0.583036440791858 1105 56-57 0.607817659530248 1105 58-59 0.6003326959530213 1105 60-61 0.6707501196190506 1105 62-63 0.7141073763162851 1105 64-65 0.636834307775537 1105 66-67 0.4088704093694737 1105 68-69 0.5476945010146572 1105 70-71 0.7793326592401435 1105 72-73 0.8681322211767544 1105 74-75 0.7543862798722785 1105 76-77 0.6916707273462883 1105 78-79 0.888352555511851 1105 80-81 0.7909295228215321 1105 82-83 0.9491855697728049 1105 84-85 0.9969864826137318 1105 86-87 1.0441935030025142 1105 88-89 1.0896161361635919 1105 90-91 1.2748851601157796 1105 92-93 1.4396109465035458 1105 94-95 1.565265217026237 1105 96-97 1.55504318073762 1105 98-99 1.4600929073512283 1105 100-101 1.572226252473616 1106 1 -0.06167024002936827 1106 2 0.08135187719182824 1106 3 0.054154754886099 1106 4 0.09323914988362247 1106 5 0.030910087906825368 1106 6 -0.08069942131890429 1106 7 -0.06675810170010976 1106 8 0.004786828874550508 1106 9 -0.28474784897122163 1106 10-11 0.043562870220497985 1106 12-13 0.02002206603675205 1106 14-15 0.007022921719702424 1106 16-17 0.019972649688831723 1106 18-19 -0.03498192525891142 1106 20-21 0.027397502709241905 1106 22-23 0.185222192861211 1106 24-25 0.16218207855070688 1106 26-27 0.004367333180802291 1106 28-29 0.13258051514935687 1106 30-31 0.09768569236531732 1106 32-33 0.08406169966993104 1106 34-35 -0.012197204742157908 1106 36-37 0.07093595896436966 1106 38-39 0.028350446174428612 1106 40-41 0.04182691547701012 1106 42-43 0.10718301952692144 1106 44-45 0.02082510730390652 1106 46-47 -0.6059352606214645 1106 48-49 -0.32436115458455816 1106 50-51 0.039490912392956545 1106 52-53 -0.05323205323475122 1106 54-55 0.16327656156196468 1106 56-57 0.05004634112488304 1106 58-59 -0.00979420845300183 1106 60-61 0.046130321731098434 1106 62-63 -0.14954363212498123 1106 64-65 -0.12601107300559278 1106 66-67 -0.1293529932812767 1106 68-69 -0.29502602693283464 1106 70-71 -0.18241391509862126 1106 72-73 0.1688841035633466 1106 74-75 0.10171107645872368 1106 76-77 0.14766059366117545 1106 78-79 0.44662050454912006 1106 80-81 -0.10690577216833219 1106 82-83 0.2769239261804586 1106 84-85 0.33083367917427964 1106 86-87 0.5375187699494575 1106 88-89 0.63938512237333 1106 90-91 0.5653967705960135 1106 92-93 0.7303277142221525 1106 94-95 1.1070155575167888 1106 96-97 1.206234747049308 1106 98-99 1.0475022983650994 1106 100-101 1.1323297154440617 1107 1 0.1090428482676451 1107 2 0.10355524583521714 1107 3 0.1163719927924376 1107 4 0.03621841775954948 1107 5 0.03165835317953025 1107 6 0.02694245701086828 1107 7 0.071285552731851 1107 8 0.15381305737136586 1107 9 0.15264364591003243 1107 10-11 0.09055849698237495 1107 12-13 0.13105297814279737 1107 14-15 0.21712977873463757 1107 16-17 0.14595674709116935 1107 18-19 0.11000315744096412 1107 20-21 0.13068128123260436 1107 22-23 0.1985349029142398 1107 24-25 0.2108385471739993 1107 26-27 0.22094728130652896 1107 28-29 0.18145773700005208 1107 30-31 0.18081022627395527 1107 32-33 0.1756278997342875 1107 34-35 0.23097054347035595 1107 36-37 0.25524396687146833 1107 38-39 0.22761537048523195 1107 40-41 0.18503763083723612 1107 42-43 0.06350377688944064 1107 44-45 0.17835614560218005 1107 46-47 0.1408134238661134 1107 48-49 0.25067296009495266 1107 50-51 0.2726625960920117 1107 52-53 0.33763412453802033 1107 54-55 0.12447176121899162 1107 56-57 0.21586535722472888 1107 58-59 0.36824587172065293 1107 60-61 0.2677927986255568 1107 62-63 0.40454844209981644 1107 64-65 0.2853162363716635 1107 66-67 0.23547076774153552 1107 68-69 0.3410090084033648 1107 70-71 0.48586518409322466 1107 72-73 0.47034013852263357 1107 74-75 0.42194981504973583 1107 76-77 0.4403011625386064 1107 78-79 0.6985131979000947 1107 80-81 0.764002640789684 1107 82-83 0.8471392562313653 1107 84-85 1.0098339134973315 1107 86-87 1.0776353753736778 1107 88-89 1.1209757671324532 1107 90-91 1.3500062377225817 1107 92-93 1.5342556197210406 1107 94-95 1.5559016912482342 1107 96-97 1.5463870950565912 1107 98-99 1.5242226121773719 1107 100-101 1.593252615555425 1108 1 0.16651337712695025 1108 2 0.17420601957781656 1108 3 0.15673603455017115 1108 4 0.12836061597097626 1108 5 0.042919644915933475 1108 6 0.1452348041194611 1108 7 0.15584896305968954 1108 8 0.15332087421823104 1108 9 0.23844977797087807 1108 10-11 0.2285436912116694 1108 12-13 0.28856480133156737 1108 14-15 0.33756734928854115 1108 16-17 0.37464958583986885 1108 18-19 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-0.9033378361739217 2316 72-73 -1.058877998337696 2316 74-75 -0.9540512044596312 2316 76-77 -0.9974414575104795 2316 78-79 -1.1402535283031519 2316 80-81 -1.1175991469117683 2316 82-83 -1.123941280900624 2316 84-85 -1.129334885134508 2316 86-87 -1.4760883669298757 2316 88-89 -1.5855450437100238 2316 90-91 -1.3726691215067852 2316 92-93 -1.6283958714092073 2316 94-95 -1.4941274445767405 2316 96-97 -1.5475960392226895 2316 98-99 -1.515337676485398 2316 100-101 -1.9359438283677477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 35.0 4 77.0 5 148.0 6 290.0 7 777.0 8 1758.0 9 3090.0 10 5093.0 11 7785.0 12 11105.0 13 15659.0 14 20286.0 15 27021.0 16 33817.0 17 41747.0 18 41029.0 19 36842.0 20 38027.0 21 41228.0 22 45016.0 23 50681.0 24 59550.0 25 69153.0 26 82993.0 27 99309.0 28 119394.0 29 143773.0 30 169616.0 31 201345.0 32 243406.0 33 298171.0 34 378198.0 35 492445.0 36 644732.0 37 730520.0 38 349595.0 39 13631.0 >>END_MODULE >>Per base sequence content fail #Base G 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22.604827568039383 18-19 22.579248395131035 27.150360598820498 28.171136731360242 22.099254274688217 20-21 23.076232576332966 26.22171818990217 28.343870860485147 22.358178373279713 22-23 22.81852600652551 26.104448031112064 28.334916489700703 22.74210947266172 24-25 22.801565129088612 26.38578999690397 28.319354696953546 22.493290177053872 26-27 22.37759900187348 26.659804842002778 28.30484964014714 22.657746515976598 28-29 22.371898439868477 27.118836546302045 28.179116775157787 22.330148238671686 30-31 22.537804913144445 26.491597801326904 28.120380229651982 22.85021705587667 32-33 22.314166949374382 26.5748498420803 28.21527772781584 22.895705480729482 34-35 23.009833686125706 26.60483999698018 27.892692670353775 22.492633646540344 36-37 23.117368831670046 26.234584883595673 28.023104282914428 22.62494200181985 38-39 22.50850091650506 26.628036199736115 28.021998335237186 22.841464548521635 40-41 22.86121134305891 26.45132047904722 28.09338542934939 22.594082748544484 42-43 22.653656918948673 26.435158594902084 27.807566478895097 23.103618007254145 44-45 22.60764733275839 26.550763505680425 27.975061991354583 22.866527170206602 46-47 22.95995857745708 26.92155373496878 27.413988785895793 22.704498901678352 48-49 22.8284433318937 26.69906695235057 27.533694002253977 22.938795713501754 50-51 22.827591191544276 26.932537521983452 27.531432640846553 22.708438645625716 52-53 23.099686608090988 26.793370090896275 27.260967554628856 22.845975746383886 54-55 22.569133792762383 26.74694149025956 27.51857951483345 23.165345202144614 56-57 22.82162517070307 26.93299850553876 27.359122511509522 22.88625381224865 58-59 22.951408424352447 26.55452102014034 27.313871638636343 23.18019891687087 60-61 22.527248847609005 26.842834964858795 27.72525555375755 22.904660633774647 62-63 22.391355435933715 26.896704712138437 27.780891379622773 22.931048472305076 64-65 22.21129236518859 26.89135604027737 27.558759974140294 23.33859162039374 66-67 22.28034150153037 26.823570641158284 27.434378377133843 23.4617094801775 68-69 22.48881658310677 26.574371258333525 27.60666963274864 23.330142525811056 70-71 22.60390926745094 26.56279659999214 27.35937113468815 23.47392299786877 72-73 22.49867150933474 26.57698566049147 27.46702183107797 23.457320999095817 74-75 22.35713182581317 27.130846041306516 27.006243416292886 23.50577871658743 76-77 22.360347217431258 26.656314120882048 27.292183975583555 23.69115468610314 78-79 22.537140235368465 26.728059883629975 27.306710292047732 23.428089588953828 80-81 22.615599704883856 26.911556826243725 27.108725323215065 23.364118145657358 82-83 22.579096104938344 26.805409016185884 27.167751405550767 23.447743473325012 84-85 22.43060551759517 26.7653591228049 27.176927631891388 23.62710772770854 86-87 22.408535252293586 26.82900991290358 27.03064198700411 23.73181284779872 88-89 22.311555039047708 26.82362848389401 26.870198379558165 23.994618097500126 90-91 22.343705646316618 26.68317592411764 27.004028117107676 23.969090312458068 92-93 22.333488586150203 26.46094764297107 27.161843810056908 24.04371996082182 94-95 22.0946862774448 26.300641519026357 27.31908508032052 24.285587123208323 96-97 21.940841558395544 26.222421036081023 27.37345731641152 24.46328008911191 98-99 22.017537982628127 26.360339818485144 26.984385027211726 24.637737171675 100-101 21.94590399944207 26.039120440455044 27.30896557602715 24.706009984075735 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 661.0 1 466.5 2 383.5 3 509.0 4 447.0 5 455.0 6 854.5 7 1787.5 8 2770.0 9 3136.0 10 2751.5 11 2367.5 12 2490.0 13 2735.5 14 2979.0 15 3227.5 16 3500.5 17 3824.5 18 4188.0 19 4556.0 20 5099.0 21 6007.5 22 7168.0 23 8816.0 24 11141.0 25 14448.0 26 18768.5 27 24111.5 28 29869.5 29 36080.5 30 43498.0 31 52514.5 32 65468.0 33 72817.5 34 75997.5 35 83602.0 36 94691.0 37 105132.5 38 112750.0 39 120097.0 40 126822.0 41 137644.0 42 147304.5 43 154819.0 44 162085.5 45 170276.5 46 177280.5 47 186771.0 48 195352.5 49 191392.0 50 182392.0 51 171026.0 52 162468.0 53 163006.0 54 166554.0 55 157463.5 56 137481.0 57 121306.5 58 109893.0 59 95599.0 60 77709.5 61 64672.5 62 54748.5 63 42173.5 64 31955.0 65 24724.5 66 17935.0 67 14255.0 68 11117.0 69 7502.5 70 5455.0 71 4155.0 72 3329.0 73 2673.5 74 2027.5 75 1596.5 76 1064.5 77 570.0 78 358.0 79 252.5 80 144.0 81 74.5 82 37.0 83 15.5 84 7.0 85 7.5 86 5.0 87 3.0 88 1.5 89 1.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.763270787034957E-4 2 0.05053851298118121 3 2.2136886982558568E-5 4 0.0 5 0.04544702897519274 6 2.2136886982558568E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006098712363694886 26-27 0.0018594985065349197 28-29 7.7479104438955E-5 30-31 0.00170454029765701 32-33 4.3166929615989205E-4 34-35 0.011644002552825806 36-37 0.02305556779233475 38-39 0.004139597865738452 40-41 0.0010404336881802527 42-43 0.0014942398713227033 44-45 0.008555906818758886 46-47 0.0 48-49 0.0 50-51 6.309012790029192E-4 52-53 0.0 54-55 0.0016270611932180548 56-57 0.0 58-59 0.021871244338767866 60-61 0.0 62-63 0.009463519185043788 64-65 0.014765303617366566 66-67 0.006541450103346056 68-69 0.0160824483928288 70-71 9.961599142151356E-5 72-73 5.534221745639642E-5 74-75 0.021904449669241703 76-77 0.04855726159624222 78-79 0.029253896147451146 80-81 0.025656652012785382 82-83 0.035208718745759406 84-85 0.016016037731881125 86-87 0.010105488907537985 88-89 0.08193968716594054 90-91 0.024195617471936517 92-93 0.023409757984055687 94-95 0.026365032396227252 96-97 0.03712355946975072 98-99 0.05068240274656784 100-101 0.014488592530084582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4517347.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.67044948749822 #Duplication Level Percentage of deduplicated Percentage of total 1 69.85861391106876 30.507570700699688 2 15.21080215209381 13.285251340946841 3 5.830906113647594 7.639148727071755 4 2.8268014790302245 4.937907648046987 5 1.6145253820884855 3.5253524572389483 6 1.0137224199201411 2.6561828240080185 7 0.6922374682288611 2.116122497274949 8 0.49268856254461 1.7212744786938 9 0.36039483693459706 1.4164744069716728 >10 1.853919239319957 14.60935465717817 >50 0.1451656611420309 4.384299608000298 >100 0.08974925014442685 7.495512702384842 >500 0.006456978641492058 1.9096427409142462 >1k 0.0038131758181501776 3.099172551996193 >5k 1.5252703272599635E-4 0.46579842078423306 >10k+ 5.084234424199878E-5 0.23093423778906566 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10401 0.23024576150559167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8325 0.1842895841298001 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6907 0.15289947838853202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5747 0.12722068948876408 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2136886982558568E-5 2 0.0 0.0 0.0 0.0 2.2136886982558568E-5 3 0.0 0.0 0.0 0.0 2.2136886982558568E-5 4 0.0 0.0 0.0 2.2136886982558568E-5 2.2136886982558568E-5 5 0.0 0.0 0.0 2.2136886982558568E-5 2.2136886982558568E-5 6 0.0 0.0 0.0 2.2136886982558568E-5 2.2136886982558568E-5 7 0.0 0.0 0.0 2.2136886982558568E-5 2.2136886982558568E-5 8 0.0 0.0 0.0 2.2136886982558568E-5 2.2136886982558568E-5 9 0.0 0.0 0.0 6.641066094767571E-5 2.2136886982558568E-5 10-11 0.0 0.0 0.0 9.961599142151357E-5 2.2136886982558568E-5 12-13 0.0 0.0 0.0 1.3282132189535142E-4 1.3282132189535142E-4 14-15 0.0 0.0 0.0 1.3282132189535142E-4 1.3282132189535142E-4 16-17 0.0 0.0 0.0 1.5495820887790997E-4 1.5495820887790997E-4 18-19 0.0 0.0 0.0 1.7709509586046854E-4 1.7709509586046854E-4 20-21 0.0 0.0 0.0 2.5457420029942356E-4 1.9923198284302712E-4 22-23 0.0 0.0 0.0 3.8739552219477496E-4 1.9923198284302712E-4 24-25 0.0 0.0 0.0 5.866275050378021E-4 1.9923198284302712E-4 26-27 0.0 0.0 0.0 0.0011179127926192077 1.9923198284302712E-4 28-29 0.0 0.0 0.0 0.0033980121518227403 1.9923198284302712E-4 30-31 0.0 0.0 0.0 0.01128981236110487 1.9923198284302712E-4 32-33 0.0 0.0 0.0 0.030050824078823257 1.9923198284302712E-4 34-35 0.0 0.0 0.0 0.05907228291295754 2.4350575680814424E-4 36-37 0.0 0.0 0.0 0.10760740762221721 2.4350575680814424E-4 38-39 0.0 0.0 0.0 0.18247435939723028 2.4350575680814424E-4 40-41 0.0 0.0 0.0 0.26462434698950515 2.4350575680814424E-4 42-43 0.0 0.0 0.0 0.3536589064333557 2.4350575680814424E-4 44-45 0.0 0.0 0.0 0.4622846108567706 2.4350575680814424E-4 46-47 0.0 0.0 0.0 0.592449506314215 2.5457420029942356E-4 48-49 0.0 0.0 0.0 0.7293882891883223 2.6564264379070284E-4 50-51 0.0 0.0 0.0 0.8785244967898194 2.6564264379070284E-4 52-53 0.0 0.0 0.0 1.0240966655871244 2.877795307732614E-4 54-55 0.0 0.0 0.0 1.1843455904538658 2.877795307732614E-4 56-57 0.0 0.0 0.0 1.3767483436627739 2.877795307732614E-4 58-59 0.0 0.0 0.0 1.581691643347301 2.877795307732614E-4 60-61 0.0 0.0 0.0 1.8031822660512908 2.877795307732614E-4 62-63 0.0 0.0 0.0 2.025857212208847 2.877795307732614E-4 64-65 0.0 0.0 0.0 2.2627551082526978 2.877795307732614E-4 66-67 0.0 0.0 0.0 2.5381711876462 3.0991641775581993E-4 68-69 0.0 0.0 0.0 2.843029326726506 3.0991641775581993E-4 70-71 0.0 0.0 0.0 3.1635603817904623 3.0991641775581993E-4 72-73 0.0 0.0 0.0 3.4910866931409075 3.431217482296578E-4 74-75 0.0 0.0 0.0 3.8264494624831786 3.763270787034957E-4 76-77 0.0 0.0 0.0 4.192538230957241 3.763270787034957E-4 78-79 0.0 0.0 0.0 4.604339671050287 3.763270787034957E-4 80-81 0.0 0.0 0.0 5.043679398549635 3.763270787034957E-4 82-83 0.0 0.0 0.0 5.488243431376867 3.763270787034957E-4 84-85 0.0 0.0 0.0 5.9359398337121325 3.763270787034957E-4 86-87 0.0 0.0 0.0 6.404522388915441 3.9846396568605423E-4 88-89 0.0 0.0 0.0 6.9140913903669565 4.3166929615989205E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19770 0.0 25.582499 1 GGTATCA 14555 0.0 25.27765 1 GTACATG 15725 0.0 24.817638 1 TACATGG 15650 0.0 24.541449 2 ACATGGG 15895 0.0 23.342808 3 ATCAACG 22540 0.0 22.257307 3 TCAACGC 22830 0.0 22.05782 4 CAACGCA 22950 0.0 21.942484 5 TATCAAC 23095 0.0 21.734472 2 AACGCAG 23555 0.0 21.387226 6 GAGTACT 11555 0.0 20.915586 12-13 GTACTTT 11710 0.0 20.33463 14-15 CATGGGG 8430 0.0 19.94979 4 TAAGGTG 2110 0.0 19.813574 5 GTAAGGT 2170 0.0 19.046804 4 AGAGTAC 19200 0.0 19.029596 10-11 ACGCAGA 26715 0.0 18.821878 7 CGCAGAG 26710 0.0 18.754297 8 AGTACTT 12205 0.0 18.712399 12-13 GAGTACA 12460 0.0 18.121058 1 >>END_MODULE