FastQCFastQC Report
Thu 26 May 2016
SRR938550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506410
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105910.23502078150900604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78160.17344183063680402No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68500.15200569854939963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54080.1200068347087815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA203750.026.6877651
GTACATG156250.026.6643941
TACATGG156750.026.2854372
ACATGGG163650.024.654453
TATCAAC237050.022.794192
ATCAACG235900.022.7642943
TCAACGC236600.022.656774
CAACGCA236750.022.6213385
GGTATCA151100.022.4052031
AACGCAG242250.022.1040656
GAGTACT109550.022.04804412-13
GTACCGT3450.022.0349926
GGACCGA13800.021.34646
CATGGGG89000.021.3052464
GTACTTT113450.020.93620714-15
AGGACCG17800.020.556785
AGAGTAC184900.020.42423410-11
TAAGGTG20850.020.2846875
AGTACTT116200.019.68256412-13
ACGCAGA274050.019.4843467
Produced by FastQC (version 0.11.5)