##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938550_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4506410 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.110811044711866 31.0 31.0 34.0 28.0 34.0 2 31.20982067765694 31.0 31.0 34.0 28.0 34.0 3 31.284370707503314 31.0 31.0 34.0 27.0 34.0 4 34.90901715556286 37.0 35.0 37.0 32.0 37.0 5 34.928449475302955 37.0 35.0 37.0 32.0 37.0 6 34.96252249573386 37.0 35.0 37.0 32.0 37.0 7 34.88685516852661 37.0 35.0 37.0 32.0 37.0 8 34.847430881788384 37.0 35.0 37.0 32.0 37.0 9 36.35788155094632 39.0 35.0 39.0 32.0 39.0 10-11 36.21159392953592 39.0 35.0 39.0 32.0 39.0 12-13 36.07228292587669 39.0 35.0 39.0 30.0 39.0 14-15 37.08139594488739 39.0 36.0 41.0 31.0 41.0 16-17 36.94567826717942 39.0 36.0 41.0 31.0 41.0 18-19 36.92776056772464 39.0 36.0 41.0 31.0 41.0 20-21 37.01285946019115 39.0 36.0 41.0 31.0 41.0 22-23 36.943339376576915 39.0 36.0 41.0 30.5 41.0 24-25 36.83564677869967 39.0 36.0 41.0 30.0 41.0 26-27 36.65861184401774 39.0 36.0 41.0 30.0 41.0 28-29 36.48968180880124 39.0 36.0 41.0 30.0 41.0 30-31 36.29882656038842 39.0 35.0 40.0 29.5 41.0 32-33 36.04574539378352 38.5 35.0 40.0 29.0 41.0 34-35 35.81105225667439 38.0 35.0 40.0 27.5 41.0 36-37 35.5438593026378 38.0 34.5 40.0 27.0 41.0 38-39 35.304750455462326 38.0 34.0 40.0 26.0 41.0 40-41 35.281004946287624 38.0 34.0 40.0 26.0 41.0 42-43 35.131140974744866 38.0 34.0 40.0 25.5 41.0 44-45 34.880226277680016 38.0 34.0 40.0 24.5 41.0 46-47 34.55159861175525 38.0 33.0 40.0 24.0 41.0 48-49 34.22357741528178 38.0 33.0 40.0 23.0 41.0 50-51 33.488768332220104 37.5 32.0 39.5 22.5 40.5 52-53 33.5524537270244 37.5 33.0 39.5 23.0 40.5 54-55 33.7041440969641 38.0 33.0 40.0 22.0 41.0 56-57 33.42161576509905 37.0 32.5 40.0 20.5 41.0 58-59 33.11005811721525 37.0 32.0 40.0 19.5 41.0 60-61 32.67145954318404 36.5 31.0 39.5 17.5 41.0 62-63 32.17181958587878 36.0 31.0 39.0 13.5 40.5 64-65 31.70002252347212 35.0 30.5 39.0 10.0 40.0 66-67 31.179021660257277 35.0 30.0 38.5 8.0 40.0 68-69 30.640636227063226 35.0 29.0 38.0 6.5 40.0 70-71 30.024457162131277 34.0 28.5 37.0 2.0 39.5 72-73 29.49582772095748 34.0 28.0 36.5 2.0 39.0 74-75 28.917147796139275 34.0 27.5 36.0 2.0 39.0 76-77 28.216456558546604 33.5 26.0 35.5 2.0 38.0 78-79 27.462593172836026 33.0 25.5 35.0 2.0 37.0 80-81 26.82815345252651 32.0 24.5 35.0 2.0 36.5 82-83 26.193824241469372 32.0 23.0 35.0 2.0 36.0 84-85 25.56394291686731 31.5 20.5 34.5 2.0 36.0 86-87 24.92743802716575 31.0 19.0 34.0 2.0 35.0 88-89 24.168594735055176 31.0 13.5 34.0 2.0 35.0 90-91 23.36598389849126 30.0 4.5 34.0 2.0 35.0 92-93 22.547109672666267 29.5 2.0 34.0 2.0 35.0 94-95 21.622638974261108 29.0 2.0 34.0 2.0 35.0 96-97 20.40831826220872 28.5 2.0 34.0 2.0 35.0 98-99 18.95214916086197 26.0 2.0 33.5 2.0 35.0 100-101 15.431656906495414 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6080395914674384 1101 2 0.6032795543597551 1101 3 0.6799020624387886 1101 4 0.508458010278936 1101 5 0.5279034128495894 1101 6 0.5252400923304634 1101 7 0.5070379686750286 1101 8 0.6076345859620389 1101 9 0.6253330784193949 1101 10-11 0.6203995008989196 1101 12-13 0.7042396282594012 1101 14-15 0.8976336548076063 1101 16-17 0.8617105094692761 1101 18-19 0.7728977213001045 1101 20-21 0.690913451338858 1101 22-23 0.7922816763574829 1101 24-25 0.8379682675960538 1101 26-27 0.8451611199875728 1101 28-29 0.8867620065013782 1101 30-31 0.7538839790717731 1101 32-33 0.8169323953196539 1101 34-35 0.8365063299919839 1101 36-37 0.9476569776890926 1101 38-39 0.8952520379748208 1101 40-41 0.8396593934838634 1101 42-43 0.9702606776090761 1101 44-45 0.9173663185669412 1101 46-47 1.0072671657234693 1101 48-49 1.0579851945947425 1101 50-51 0.9805380278450642 1101 52-53 1.0368458559653533 1101 54-55 1.0443675333186064 1101 56-57 0.9763337678256505 1101 58-59 0.9725323683777276 1101 60-61 0.9722180884444 1101 62-63 0.9797375374396466 1101 64-65 0.9880613222466756 1101 66-67 0.906083295429216 1101 68-69 0.9312866068097172 1101 70-71 1.1722445721572292 1101 72-73 1.159560630618337 1101 74-75 1.0328615172286852 1101 76-77 1.1171768263221757 1101 78-79 1.010035494611369 1101 80-81 1.091887418304239 1101 82-83 1.2632842724815028 1101 84-85 1.2821924620144678 1101 86-87 1.4349663882868207 1101 88-89 1.4615581081169005 1101 90-91 1.361918479767084 1101 92-93 1.2689814799997912 1101 94-95 1.1970646941288514 1101 96-97 1.3390284630715463 1101 98-99 1.4989228753174935 1101 100-101 1.3464241474332503 1102 1 0.5219477570975677 1102 2 0.5723097656859757 1102 3 0.5480079039957779 1102 4 0.4705841318222852 1102 5 0.5177313093930778 1102 6 0.558871480085763 1102 7 0.42721550676377973 1102 8 0.4254594181953735 1102 9 0.6177574650687276 1102 10-11 0.5909787474065737 1102 12-13 0.6049540247550453 1102 14-15 0.6519198053288946 1102 16-17 0.6978069763491064 1102 18-19 0.74346433945869 1102 20-21 0.6594712101076894 1102 22-23 0.7061602066156425 1102 24-25 0.6220724252039531 1102 26-27 0.5953999163574082 1102 28-29 0.5030533002509969 1102 30-31 0.5126370114620258 1102 32-33 0.5829136028131998 1102 34-35 0.5478028514973659 1102 36-37 0.5320721607468499 1102 38-39 0.5302626699024842 1102 40-41 0.5869481694325813 1102 42-43 0.5672828875117872 1102 44-45 0.5935095141459286 1102 46-47 0.5304144853785857 1102 48-49 0.3635228335478331 1102 50-51 0.5486247555390307 1102 52-53 0.6037394267776506 1102 54-55 0.5088010255787552 1102 56-57 0.35945960278797884 1102 58-59 0.5774182956754998 1102 60-61 0.6188096145302566 1102 62-63 0.3564198048768574 1102 64-65 0.46110316028448395 1102 66-67 0.626457949989927 1102 68-69 0.537655508427644 1102 70-71 0.5186038254626233 1102 72-73 0.5045643353571272 1102 74-75 0.29933827637040267 1102 76-77 0.33730183301710426 1102 78-79 0.4296898129102402 1102 80-81 0.5921997002431105 1102 82-83 0.5581360435852503 1102 84-85 0.48288243947127896 1102 86-87 0.43758540635208476 1102 88-89 0.6139211704877461 1102 90-91 0.7235414091937784 1102 92-93 0.5879247143649415 1102 94-95 0.7086806176774587 1102 96-97 0.9683232122928409 1102 98-99 0.8624270480032479 1102 100-101 0.7212798733044075 1103 1 0.6237864307999708 1103 2 0.6267923927902999 1103 3 0.5036636903745801 1103 4 0.5094045998307664 1103 5 0.5465781131375351 1103 6 0.5358021236819113 1103 7 0.5731384161987805 1103 8 0.5230212504480818 1103 9 0.4729134518018938 1103 10-11 0.5936707979248368 1103 12-13 0.5901853133473978 1103 14-15 0.7367025898200197 1103 16-17 0.6778455868071305 1103 18-19 0.7039797450193603 1103 20-21 0.5902831763322922 1103 22-23 0.7310515807568905 1103 24-25 0.840497483017046 1103 26-27 0.739702309022995 1103 28-29 0.6807527735362768 1103 30-31 0.7970396657952321 1103 32-33 0.8910241525565965 1103 34-35 0.8881087177765252 1103 36-37 0.8964658112033135 1103 38-39 0.806138375367091 1103 40-41 1.0411280321294925 1103 42-43 0.8445701256631182 1103 44-45 0.9708238484142484 1103 46-47 1.0405427001005236 1103 48-49 0.9723014323225314 1103 50-51 0.9391747282618681 1103 52-53 0.9558182239755837 1103 54-55 1.0591644997716685 1103 56-57 1.0139184528987784 1103 58-59 0.9724738879760935 1103 60-61 0.9840072170368543 1103 62-63 1.0293693710975944 1103 64-65 0.9864416505298799 1103 66-67 1.1167093826443057 1103 68-69 1.1066898366129188 1103 70-71 1.176712685470445 1103 72-73 1.2695778773808506 1103 74-75 1.1274994095553588 1103 76-77 0.9848678292610593 1103 78-79 0.9689522071207222 1103 80-81 1.001667831830062 1103 82-83 1.0713652851836422 1103 84-85 0.9936500984150491 1103 86-87 1.064817969487848 1103 88-89 1.0712048964029819 1103 90-91 1.2139988085458846 1103 92-93 1.212496969872273 1103 94-95 1.1224924559649345 1103 96-97 0.9546855374655543 1103 98-99 1.073243226497258 1103 100-101 0.7548896192164563 1104 1 0.5615355275418352 1104 2 0.5539037515330847 1104 3 0.5335151418800841 1104 4 0.48712232565674185 1104 5 0.44925018981894027 1104 6 0.490201088052693 1104 7 0.49893201342001703 1104 8 0.43954263690069695 1104 9 0.50882565671278 1104 10-11 0.5558444465843095 1104 12-13 0.5846848488473313 1104 14-15 0.6774466537150943 1104 16-17 0.6801614800316074 1104 18-19 0.7632339649910378 1104 20-21 0.7378726383588372 1104 22-23 0.7817050270956898 1104 24-25 0.7676516592845246 1104 26-27 0.7173479858545093 1104 28-29 0.7561230006836723 1104 30-31 0.6954971480218077 1104 32-33 0.6794238640173944 1104 34-35 0.6751778253185776 1104 36-37 0.7609802942238986 1104 38-39 0.7972470603742678 1104 40-41 0.8035819253493912 1104 42-43 0.7234153796393983 1104 44-45 0.7349098701656587 1104 46-47 0.9035511331793415 1104 48-49 0.8463074193394107 1104 50-51 0.7480999082842459 1104 52-53 0.8493355020051894 1104 54-55 1.0943259249860162 1104 56-57 1.1297424027968361 1104 58-59 0.9957111331017074 1104 60-61 1.053610321178688 1104 62-63 1.0813137214725188 1104 64-65 1.119140653342047 1104 66-67 1.2066422017478509 1104 68-69 1.1909611607126926 1104 70-71 1.1000940571871922 1104 72-73 1.1464838721775372 1104 74-75 1.0790769936516114 1104 76-77 1.2116285533777678 1104 78-79 1.2675107981982023 1104 80-81 1.0932396439981353 1104 82-83 1.0380116347393944 1104 84-85 1.0288156423492048 1104 86-87 1.05206638435277 1104 88-89 1.2344666021479078 1104 90-91 1.1332384642666717 1104 92-93 1.0914167326340127 1104 94-95 1.0983427141857867 1104 96-97 1.107546817838255 1104 98-99 0.9461696512882902 1104 100-101 0.6556612213298187 1105 1 0.5609020204046828 1105 2 0.5885886976637273 1105 3 0.5087599467153971 1105 4 0.5083785451329632 1105 5 0.4703134951822392 1105 6 0.5153523327710232 1105 7 0.3857904684189819 1105 8 0.4396714256308485 1105 9 0.6019054250845812 1105 10-11 0.5418129574708033 1105 12-13 0.6422647084848307 1105 14-15 0.7328456047809198 1105 16-17 0.6743348121247479 1105 18-19 0.7538690230801848 1105 20-21 0.7730364267982281 1105 22-23 0.7737429355258243 1105 24-25 0.7677004257773774 1105 26-27 0.7987438620221496 1105 28-29 0.6986584806608178 1105 30-31 0.7339679394748231 1105 32-33 0.7918996509022804 1105 34-35 0.8247585383831577 1105 36-37 0.8499915168652308 1105 38-39 0.7937141660164713 1105 40-41 0.767448966747871 1105 42-43 0.7634232436062192 1105 44-45 0.6792486882455577 1105 46-47 0.7375285536206917 1105 48-49 0.715568890211074 1105 50-51 0.601563590803778 1105 52-53 0.5785453078306233 1105 54-55 0.6110377421266762 1105 56-57 0.6802290106248776 1105 58-59 0.835359184811729 1105 60-61 0.9014353544262477 1105 62-63 0.9830735005799411 1105 64-65 1.0177500309368916 1105 66-67 0.9716617032343215 1105 68-69 0.7046516609514661 1105 70-71 0.928027525811455 1105 72-73 0.9736684671517715 1105 74-75 0.9015224968941133 1105 76-77 0.9850442031881919 1105 78-79 1.0431568762236232 1105 80-81 0.9128692521291022 1105 82-83 0.9855000473849422 1105 84-85 1.0386979717338605 1105 86-87 1.0586069313110826 1105 88-89 0.9748692191736374 1105 90-91 0.997084003219264 1105 92-93 1.1851856874811908 1105 94-95 1.2653534809559055 1105 96-97 1.4251364641281299 1105 98-99 1.4155413618699946 1105 100-101 1.114676225324473 1106 1 0.2610487253762095 1106 2 0.37491631030789563 1106 3 0.13288267243346752 1106 4 0.33982340208189044 1106 5 0.3849577058376923 1106 6 0.005323054980358677 1106 7 0.18082541847722666 1106 8 0.315179891136637 1106 9 0.27750989795431025 1106 10-11 0.11159775322410326 1106 12-13 0.18317694215013347 1106 14-15 0.4473660606387213 1106 16-17 0.18164275880118907 1106 18-19 -0.14025819838526132 1106 20-21 0.2857299192997331 1106 22-23 0.26608115208284033 1106 24-25 0.3272168837459759 1106 26-27 0.07525097610979259 1106 28-29 0.15851797897105513 1106 30-31 0.2640097947896791 1106 32-33 0.38766393987221903 1106 34-35 0.366952769797372 1106 36-37 0.21873993871699327 1106 38-39 0.23765712297075225 1106 40-41 0.37235646511363996 1106 42-43 0.03798516208740921 1106 44-45 0.29857418004498726 1106 46-47 0.14188271966784072 1106 48-49 0.3985734730965831 1106 50-51 0.1481858847166606 1106 52-53 0.4067488283147682 1106 54-55 0.14629221396545944 1106 56-57 -0.22227898748481323 1106 58-59 -0.6717652122799507 1106 60-61 0.009144276386642503 1106 62-63 0.12298592002249364 1106 64-65 0.31980353789441196 1106 66-67 -0.28686760196185546 1106 68-69 -0.4225707370091065 1106 70-71 -0.32896056900602133 1106 72-73 0.040787260878200726 1106 74-75 0.13117048474409287 1106 76-77 0.16403442247684197 1106 78-79 0.3868962397505413 1106 80-81 0.23047668251469844 1106 82-83 0.2513691083021605 1106 84-85 0.34640874835842084 1106 86-87 0.5791064617909321 1106 88-89 0.3642093607477186 1106 90-91 0.47169523090022025 1106 92-93 0.3821692834429129 1106 94-95 0.030811925443888555 1106 96-97 0.3202067926824057 1106 98-99 0.13328232807542761 1106 100-101 -0.06886874867551818 1107 1 0.38089822249200367 1107 2 0.4010037801084856 1107 3 0.30595908201891575 1107 4 0.31729878065470274 1107 5 0.26975173658242824 1107 6 0.18896457611620576 1107 7 0.23203254890787406 1107 8 0.2974389562906836 1107 9 0.3270548320283666 1107 10-11 0.42860606777586696 1107 12-13 0.34594855591104334 1107 14-15 0.4547680647030248 1107 16-17 0.48400248197744844 1107 18-19 0.4354559239898279 1107 20-21 0.5187262115000593 1107 22-23 0.5225295535740955 1107 24-25 0.4420565868676931 1107 26-27 0.4475737660449326 1107 28-29 0.5816504813871575 1107 30-31 0.6036490235039622 1107 32-33 0.5735091218990789 1107 34-35 0.6667410743786135 1107 36-37 0.6676897899658485 1107 38-39 0.6020381803973862 1107 40-41 0.6838491721164743 1107 42-43 0.6741291374679861 1107 44-45 0.6798477865929513 1107 46-47 0.6947313845362117 1107 48-49 0.7022547766176856 1107 50-51 0.7269989281434661 1107 52-53 0.696789866553452 1107 54-55 0.8063716163748396 1107 56-57 0.7411679262504762 1107 58-59 0.7475641033004692 1107 60-61 0.7404530888882093 1107 62-63 0.7610142135005802 1107 64-65 0.5932114869185021 1107 66-67 0.7276554040700596 1107 68-69 0.6398950118869102 1107 70-71 0.6430434111886605 1107 72-73 0.7464209372336192 1107 74-75 0.9135504991472843 1107 76-77 0.9492677378428631 1107 78-79 0.7532974948997619 1107 80-81 0.7489896395673448 1107 82-83 0.8739849551576846 1107 84-85 0.7964541214432295 1107 86-87 0.7748184558661215 1107 88-89 0.8361705988900994 1107 90-91 0.8480267998768447 1107 92-93 0.853201444343167 1107 94-95 0.789172275764404 1107 96-97 0.7259704704081145 1107 98-99 0.4456455742922394 1107 100-101 0.16540425449551321 1108 1 0.30103189735134706 1108 2 0.2539373214278946 1108 3 0.31595365445204493 1108 4 0.27959690554817485 1108 5 0.30118421812006346 1108 6 0.2681139719569501 1108 7 0.3250898170544261 1108 8 0.20232548495302183 1108 9 0.20585553621265973 1108 10-11 0.29665743634367203 1108 12-13 0.3739229977096983 1108 14-15 0.43018249045282886 1108 16-17 0.3130721873522262 1108 18-19 0.32462434298067677 1108 20-21 0.4070954466817156 1108 22-23 0.43342387501359525 1108 24-25 0.4000584691717677 1108 26-27 0.5015021379339615 1108 28-29 0.2812162851541089 1108 30-31 0.42918340317664416 1108 32-33 0.34711319176741284 1108 34-35 0.4945879704070393 1108 36-37 0.4571471686602706 1108 38-39 0.510163357857671 1108 40-41 0.39319437380230937 1108 42-43 0.4257296132997297 1108 44-45 0.48467579581001274 1108 46-47 0.5974542708950352 1108 48-49 0.6010657761765756 1108 50-51 0.530749697299477 1108 52-53 0.5198306058512401 1108 54-55 0.43543881012676167 1108 56-57 0.563806053770449 1108 58-59 0.7262754329121321 1108 60-61 0.7343582781791085 1108 62-63 0.755022944558057 1108 64-65 0.5683683687628793 1108 66-67 0.6164747257158183 1108 68-69 0.5890096693851952 1108 70-71 0.5756544956869014 1108 72-73 0.4961181408127793 1108 74-75 0.5669538142250836 1108 76-77 0.6742279860550191 1108 78-79 0.556246869471483 1108 80-81 0.6706302425628685 1108 82-83 0.7987558333874141 1108 84-85 0.7686382628741271 1108 86-87 0.7591784754431039 1108 88-89 0.7427702275619303 1108 90-91 0.7236710641894639 1108 92-93 0.7836351432657729 1108 94-95 0.8441267208058321 1108 96-97 0.7761348495151132 1108 98-99 0.588466068423724 1108 100-101 0.43795604099097574 1109 1 0.5635475376607388 1109 2 0.6231314120030405 1109 3 0.5649704369237227 1109 4 0.5630192459090182 1109 5 0.5080526883129011 1109 6 0.5667491297054212 1109 7 0.4683438513678979 1109 8 0.5019914884791774 1109 9 0.5647329511715213 1109 10-11 0.45782187601498947 1109 12-13 0.35225941116750903 1109 14-15 0.4747365165249775 1109 16-17 0.5860165241690609 1109 18-19 0.5315778560718059 1109 20-21 0.4693606307249283 1109 22-23 0.5221205655957419 1109 24-25 0.563540245643253 1109 26-27 0.4901800683626689 1109 28-29 0.5686693818355835 1109 30-31 0.503638859786804 1109 32-33 0.3977886159898958 1109 34-35 0.506819050416631 1109 36-37 0.6628526736969889 1109 38-39 0.596751421281553 1109 40-41 0.5942377106959142 1109 42-43 0.5498850131128776 1109 44-45 0.6293731410017713 1109 46-47 0.6659900186139325 1109 48-49 0.6496506290079864 1109 50-51 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2316 50-51 -1.1298849003944866 2316 52-53 -1.036027434946817 2316 54-55 -1.0331924347007657 2316 56-57 -0.8904042077865171 2316 58-59 -1.0070886507360726 2316 60-61 -0.8799834993719742 2316 62-63 -1.0102075254781369 2316 64-65 -1.0857464713845992 2316 66-67 -0.8901085409539 2316 68-69 -0.8510592407088033 2316 70-71 -1.235324784247016 2316 72-73 -0.9049144205003792 2316 74-75 -0.7447687480567815 2316 76-77 -0.720935102418597 2316 78-79 -0.7775502741233424 2316 80-81 -0.8388715088993557 2316 82-83 -0.6313757656617014 2316 84-85 -0.7249262923827224 2316 86-87 -0.7480008905326017 2316 88-89 -0.7942643959110249 2316 90-91 -0.8397624111120194 2316 92-93 -1.017640158261809 2316 94-95 -1.1167319536858926 2316 96-97 -1.249371360722808 2316 98-99 -1.0904740307934553 2316 100-101 -1.0802095847330104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12710.0 3 4473.0 4 4691.0 5 5386.0 6 6289.0 7 7896.0 8 10100.0 9 12785.0 10 16318.0 11 20222.0 12 24761.0 13 28644.0 14 32865.0 15 38266.0 16 43478.0 17 49716.0 18 48972.0 19 47311.0 20 50593.0 21 54938.0 22 61058.0 23 68176.0 24 76974.0 25 87069.0 26 98550.0 27 112678.0 28 129319.0 29 150115.0 30 174329.0 31 205214.0 32 243751.0 33 295159.0 34 363965.0 35 456220.0 36 559155.0 37 585580.0 38 300756.0 39 17928.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76113917541702 18.25445328032251 14.040657587333246 23.943749956927224 2 15.49047311193916 19.82341777997222 39.88138900038719 24.804720107701435 3 19.490720184708284 23.47466921232573 29.1320930645591 27.90251753840689 4 12.968883870281633 15.924262348730398 36.27333332297247 34.83352045801551 5 14.119891150952563 37.09164954798692 33.807172171732105 14.981287129328406 6 33.34066821269048 35.25638178913029 17.04158188256673 14.361368115612501 7 29.72717509391106 31.9188624385488 20.111663263625374 18.242299203914765 8 26.755987739496263 34.76338506399294 19.417466245433832 19.06316095107697 9 26.590024994455668 16.9118050241542 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22.971981562666475 34-35 23.10722931862642 26.57330206083041 27.806230367962538 22.51323825258063 36-37 23.203306481968767 26.147517590994244 27.878405488720997 22.770770438315992 38-39 22.55427816121804 26.58417429385397 27.88499677599431 22.97655076893368 40-41 22.9217504600107 26.42769786009016 27.94968404090266 22.700867638996478 42-43 22.673043660068977 26.376921637646 27.69943715046631 23.250597551818714 44-45 22.636583736665624 26.51328943835285 27.900219918734372 22.949906906247154 46-47 22.966980349554554 26.938071441134387 27.31036296481651 22.78458524449455 48-49 22.908799453517776 26.672245265413064 27.461541901464752 22.95741337960441 50-51 22.856928539891282 26.913771815756466 27.464437166992877 22.764862477359372 52-53 23.06197802149027 26.818564389532384 27.229417270445964 22.890040318531376 54-55 22.57269794375823 26.733950459604532 27.517716063177954 23.175635533459285 56-57 22.778616037040138 26.971929398621537 27.35521570978984 22.894238854548494 58-59 22.923352507625204 26.515749798627215 27.38396916164007 23.176928532107517 60-61 22.522610994357777 26.783743275679416 27.773470498987056 22.920175230975744 62-63 22.47159618904325 26.81544998660185 27.81727690685148 22.895676917503426 64-65 22.246680442076592 26.850797930004617 27.63323781090376 23.269283817015037 66-67 22.312949641086025 26.8048500820974 27.450167357080733 23.43203291973584 68-69 22.535087646296777 26.541379205122016 27.699125894979794 23.224407253601417 70-71 22.62528510206854 26.50475961756149 27.500708038613187 23.36924724175679 72-73 22.547506440394475 26.554622557483032 27.617246030282228 23.280624971840265 74-75 22.427698452288276 27.076448957917755 27.24615180592262 23.24970078387135 76-77 22.560800431703676 26.605847463147224 27.333920197626714 23.49943190752238 78-79 22.752981317355303 26.66351544404222 27.398294012399617 23.18520922620286 80-81 22.80291803957185 26.89617369314869 27.19681010717121 23.10409816010825 82-83 22.78812444554911 26.785175141293088 27.261235900511466 23.165464512646334 84-85 22.738335305068613 26.75643665603461 27.2619198998354 23.243308139061376 86-87 22.764695034223887 26.773704673015004 27.11922802677193 23.34237226598918 88-89 22.700181782404275 26.752168065096726 27.037665993394395 23.509984159104608 90-91 22.73226182285237 26.635921110800208 27.114563508522917 23.517253557824507 92-93 22.88200872258489 26.33531912211275 27.309243811440435 23.473428343861926 94-95 22.68440940400447 26.25927937674819 27.47659997731418 23.57971124193316 96-97 22.654224411658156 26.115993734772015 27.473948159145316 23.755833694424517 98-99 22.77006407978556 26.22007614288121 27.047036307143507 23.962823470189726 100-101 23.182218142154394 25.648362163685544 27.300023139227836 23.869396554932226 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 721.0 1 497.0 2 412.0 3 550.0 4 461.0 5 463.5 6 860.5 7 1770.0 8 2832.0 9 3248.0 10 2902.5 11 2556.5 12 2614.0 13 2833.0 14 3108.5 15 3304.5 16 3484.5 17 3772.0 18 4135.5 19 4607.5 20 5160.5 21 5974.5 22 7270.5 23 9006.5 24 11083.0 25 14258.0 26 18610.0 27 24093.0 28 29571.0 29 35751.5 30 43340.5 31 52187.0 32 64324.5 33 71452.0 34 75257.0 35 83132.0 36 94164.5 37 104798.5 38 112781.5 39 119752.0 40 126513.5 41 136741.0 42 146958.5 43 154918.0 44 160664.5 45 168411.5 46 175900.5 47 184691.0 48 192947.0 49 190525.0 50 181417.0 51 169762.5 52 162173.5 53 161520.0 54 163181.5 55 154349.0 56 135727.0 57 121287.5 58 110549.0 59 96197.0 60 79178.5 61 65854.0 62 55559.0 63 43891.5 64 33562.0 65 26379.0 66 19756.0 67 15391.0 68 12069.0 69 8512.5 70 6415.5 71 4916.5 72 3774.5 73 2936.0 74 2173.0 75 1686.5 76 1141.5 77 700.5 78 470.0 79 315.5 80 205.5 81 118.0 82 66.0 83 42.5 84 29.5 85 21.0 86 14.5 87 12.5 88 10.5 89 7.5 90 6.0 91 5.0 92 4.5 93 2.5 94 0.0 95 0.5 96 1.0 97 1.5 98 1.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18216274151708345 2 0.04673343082409279 3 0.04460313198310851 4 0.05066116931215757 5 0.056763587867060476 6 0.062444384776351905 7 0.03586003048990216 8 0.03412916268160243 9 0.04018720001065149 10-11 0.04160739923797435 12-13 0.03784609034686147 14-15 0.033130585099891044 16-17 0.03154395627561629 18-19 0.03914424120308627 20-21 0.04890811089093091 22-23 0.04570156732299103 24-25 0.06580626263478023 26-27 0.051260315861184395 28-29 0.04304978907822413 30-31 0.05192603424899199 32-33 0.04836444087422139 34-35 0.04734367267958308 36-37 0.04539089874201415 38-39 0.025408251801323008 40-41 0.023965861961073226 42-43 0.020925747990085234 44-45 0.004438122585383931 46-47 0.011816501383584716 48-49 0.011084211156996367 50-51 0.009020484154792839 52-53 0.009264580896988957 54-55 0.010451778688579157 56-57 0.00841024229930255 58-59 0.01028534909162726 60-61 0.010462873995042617 62-63 0.004271692988432034 64-65 0.006945661846125852 66-67 0.003927738488064779 68-69 0.008266003315277572 70-71 0.022323756604481174 72-73 0.019150498955931662 74-75 0.01969416897264119 76-77 0.012016216899926994 78-79 0.004937411376239623 80-81 0.006712660410393196 82-83 0.00582503589331641 84-85 0.005037269134410762 86-87 0.0077001426856411195 88-89 0.028814510885605174 90-91 0.03518321679563111 92-93 0.021735705361917802 94-95 0.030911523807199078 96-97 0.03430668758501779 98-99 0.020703841860816037 100-101 0.11972945204719501 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4506410.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.21994112009742 #Duplication Level Percentage of deduplicated Percentage of total 1 72.3381551598722 33.434652722257646 2 14.033001586788476 12.972090141591941 3 5.3576001130806485 7.428838853148447 4 2.5897724680109078 4.787965239436541 5 1.4647836974295028 3.3851108124435108 6 0.9447969588234495 2.6201075884360163 7 0.6128219926474832 1.9827217492087212 8 0.4326443261892343 1.5997436225928505 9 0.324731836395413 1.3508177722215384 >10 1.6841785795959316 14.003819281801347 >50 0.1275813464749809 4.058061020001012 >100 0.08097028029998032 7.109182758994565 >500 0.00544444627815516 1.7109650278036908 >1k 0.003324484381397362 2.873038426003295 >5k 1.4454279919118139E-4 0.447031898581459 >10k+ 4.818093306372712E-5 0.2358530854775447 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10591 0.23502078150900604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7816 0.17344183063680402 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6850 0.15200569854939963 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5408 0.1200068347087815 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.657183878075896E-5 0.0 12-13 0.0 0.0 0.0 1.1095306463459827E-4 5.5476532317299136E-5 14-15 0.0 0.0 0.0 1.2204837109805809E-4 6.657183878075896E-5 16-17 0.0 0.0 0.0 1.3314367756151791E-4 6.657183878075896E-5 18-19 0.0 0.0 0.0 2.5519204865957605E-4 8.876245170767861E-5 20-21 0.0 0.0 0.0 3.439545003672546E-4 8.876245170767861E-5 22-23 0.0 0.0 0.0 4.6600287146531276E-4 8.876245170767861E-5 24-25 0.0 0.0 0.0 8.32147984759487E-4 8.876245170767861E-5 26-27 0.0 1.1095306463459827E-5 0.0 0.0013203414691517193 1.1095306463459827E-4 28-29 0.0 2.2190612926919654E-5 0.0 0.0038056901169667206 1.1095306463459827E-4 30-31 0.0 2.2190612926919654E-5 0.0 0.012693030594198042 1.1095306463459827E-4 32-33 0.0 2.2190612926919654E-5 0.0 0.03284210713184109 1.1095306463459827E-4 34-35 0.0 2.2190612926919654E-5 0.0 0.06213371619537503 1.5533429048843759E-4 36-37 0.0 2.2190612926919654E-5 0.0 0.11200711874862695 1.5533429048843759E-4 38-39 2.2190612926919654E-5 2.2190612926919654E-5 0.0 0.18919716581491697 1.5533429048843759E-4 40-41 2.2190612926919654E-5 2.2190612926919654E-5 0.0 0.2749084082451441 1.5533429048843759E-4 42-43 2.2190612926919654E-5 2.2190612926919654E-5 0.0 0.36469162814746103 1.5533429048843759E-4 44-45 2.2190612926919654E-5 2.2190612926919654E-5 0.0 0.4751564992976671 1.5533429048843759E-4 46-47 2.2190612926919654E-5 2.2190612926919654E-5 0.0 0.6082779862462582 1.5533429048843759E-4 48-49 2.2190612926919654E-5 2.2190612926919654E-5 0.0 0.7471912231687752 1.5533429048843759E-4 50-51 2.2190612926919654E-5 2.2190612926919654E-5 0.0 0.8991636357987844 1.7752490341535723E-4 52-53 2.2190612926919654E-5 2.2190612926919654E-5 0.0 1.046919831972679 1.997155163422769E-4 54-55 2.2190612926919654E-5 2.2190612926919654E-5 0.0 1.2100652182113922 1.997155163422769E-4 56-57 2.2190612926919654E-5 2.2190612926919654E-5 0.0 1.4039113174344988 1.997155163422769E-4 58-59 2.2190612926919654E-5 2.2190612926919654E-5 0.0 1.610272922348388 1.997155163422769E-4 60-61 2.2190612926919654E-5 2.2190612926919654E-5 0.0 1.83703879584858 1.997155163422769E-4 62-63 2.2190612926919654E-5 2.2190612926919654E-5 0.0 2.0624621372666936 1.997155163422769E-4 64-65 2.2190612926919654E-5 2.2190612926919654E-5 0.0 2.296562008339232 2.108108228057367E-4 66-67 2.2190612926919654E-5 2.2190612926919654E-5 0.0 2.56561209477167 2.2190612926919654E-4 68-69 2.2190612926919654E-5 2.2190612926919654E-5 0.0 2.870710831903888 2.4409674219611619E-4 70-71 2.2190612926919654E-5 2.2190612926919654E-5 0.0 3.1928963409898343 2.4409674219611619E-4 72-73 2.2190612926919654E-5 2.2190612926919654E-5 0.0 3.521328507614709 2.4409674219611619E-4 74-75 2.2190612926919654E-5 2.2190612926919654E-5 0.0 3.8571501483442474 2.55192048659576E-4 76-77 2.2190612926919654E-5 2.2190612926919654E-5 0.0 4.21613878896949 2.6628735512303583E-4 78-79 2.2190612926919654E-5 2.2190612926919654E-5 0.0 4.615303534298921 2.6628735512303583E-4 80-81 2.2190612926919654E-5 2.2190612926919654E-5 0.0 5.044325749321522 2.6628735512303583E-4 82-83 2.2190612926919654E-5 2.2190612926919654E-5 0.0 5.4856526592121 2.6628735512303583E-4 84-85 2.2190612926919654E-5 3.328591939037948E-5 0.0 5.92977558633147 2.6628735512303583E-4 86-87 2.2190612926919654E-5 4.438122585383931E-5 0.0 6.3936481589557985 2.6628735512303583E-4 88-89 2.2190612926919654E-5 4.438122585383931E-5 0.0 6.896642782170287 2.6628735512303583E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 20375 0.0 26.687765 1 GTACATG 15625 0.0 26.664394 1 TACATGG 15675 0.0 26.285437 2 ACATGGG 16365 0.0 24.65445 3 TATCAAC 23705 0.0 22.79419 2 ATCAACG 23590 0.0 22.764294 3 TCAACGC 23660 0.0 22.65677 4 CAACGCA 23675 0.0 22.621338 5 GGTATCA 15110 0.0 22.405203 1 AACGCAG 24225 0.0 22.104065 6 GAGTACT 10955 0.0 22.048044 12-13 GTACCGT 345 0.0 22.034992 6 GGACCGA 1380 0.0 21.3464 6 CATGGGG 8900 0.0 21.305246 4 GTACTTT 11345 0.0 20.936207 14-15 AGGACCG 1780 0.0 20.55678 5 AGAGTAC 18490 0.0 20.424234 10-11 TAAGGTG 2085 0.0 20.284687 5 AGTACTT 11620 0.0 19.682564 12-13 ACGCAGA 27405 0.0 19.484346 7 >>END_MODULE