FastQCFastQC Report
Thu 26 May 2016
SRR938550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506410
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104470.2318253332475296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83810.18597952694051362No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68060.15102931158061517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.1305251852361414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146650.026.0131021
GTACATG157850.025.3426021
GTATCAA198450.024.8797531
TACATGG157650.024.6805212
ACATGGG160550.023.4511343
TAAGGTG21650.023.2753855
GTAAGGT20000.021.3924964
ATCAACG230900.021.2061713
TCAACGC234050.020.9004524
TATCAAC236350.020.7892912
CAACGCA235750.020.7699035
AACGCAG243700.020.1130456
CATGGGG88150.019.8460674
GAGTACT118650.019.61471612-13
AGTACTT125100.019.2678112-13
AGAGTAC197300.019.23416510-11
GAGTACA124800.018.5234341
GTACTTT124050.018.45456914-15
GTGTAAG18700.018.3143161
GGTAAGG22850.018.308193
Produced by FastQC (version 0.11.5)