FastQCFastQC Report
Thu 26 May 2016
SRR938549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510288
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107270.23783403631874506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81030.17965593327964866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68530.15194151681666448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56570.12542436314488123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA193800.027.0252461
GTACATG160900.026.4497261
TACATGG160350.025.87322
ACATGGG164100.024.0945953
GGTATCA142350.023.736381
ATCAACG227300.022.517553
TATCAAC230250.022.4354522
TCAACGC229450.022.2444214
CAACGCA230200.022.1513025
AACGCAG238150.021.5289466
GAGTACT113500.020.59107412-13
CATGGGG91600.020.4930464
GGACCGA14050.019.6156866
AGAGTAC190800.019.6057910-11
GTACTTT118850.019.48561514-15
AGTACTT119900.019.33349612-13
ACGCAGA266400.019.063347
CGCAGAG265150.019.0277928
TAAGGTG22800.018.7591235
GAGTACA122900.018.7292771
Produced by FastQC (version 0.11.5)