##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938549_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4510288 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14447480959087 31.0 31.0 34.0 28.0 34.0 2 31.29241369952429 31.0 31.0 34.0 28.0 34.0 3 31.35829729720142 31.0 31.0 34.0 28.0 34.0 4 35.018452258481055 37.0 35.0 37.0 32.0 37.0 5 35.02340249669201 37.0 35.0 37.0 32.0 37.0 6 35.033296321653964 37.0 35.0 37.0 32.0 37.0 7 34.94899106221155 37.0 35.0 37.0 32.0 37.0 8 34.90482647671279 37.0 35.0 37.0 32.0 37.0 9 36.40139609710067 39.0 37.0 39.0 32.0 39.0 10-11 36.260286039383736 39.0 35.0 39.0 32.0 39.0 12-13 36.09785550279716 39.0 35.0 39.0 30.5 39.0 14-15 37.108812231059304 39.0 36.0 41.0 31.0 41.0 16-17 36.94327391066823 39.0 36.0 41.0 31.0 41.0 18-19 36.80453875672684 39.0 36.0 41.0 30.5 41.0 20-21 36.78536226511478 39.0 36.0 40.5 30.0 41.0 22-23 36.89081561975644 39.0 36.0 41.0 30.0 41.0 24-25 36.81059834759998 39.0 36.0 41.0 30.0 41.0 26-27 36.62735683397601 39.0 36.0 41.0 30.0 41.0 28-29 36.45283471920197 39.0 36.0 41.0 30.0 41.0 30-31 36.215088925585235 39.0 35.0 40.0 29.0 41.0 32-33 35.94028840286917 38.0 35.0 40.0 28.5 41.0 34-35 35.690736600412215 38.0 35.0 40.0 27.0 41.0 36-37 35.426026453299656 38.0 34.0 40.0 26.5 41.0 38-39 35.12133493470927 38.0 34.0 40.0 25.5 41.0 40-41 35.0532065801563 38.0 34.0 40.0 25.0 41.0 42-43 34.85014970219197 38.0 33.5 40.0 24.5 41.0 44-45 34.57445744041179 38.0 33.0 40.0 24.0 41.0 46-47 34.21711884030465 38.0 33.0 40.0 23.5 41.0 48-49 33.859880566385115 38.0 33.0 40.0 22.5 41.0 50-51 33.08040007644745 37.0 31.5 39.5 20.5 40.5 52-53 33.10808145732601 37.0 32.0 39.0 20.5 40.0 54-55 33.20305033736205 37.0 32.0 40.0 19.5 41.0 56-57 32.88562515298358 37.0 31.5 40.0 17.5 41.0 58-59 32.46505467056649 36.5 31.0 39.5 14.0 41.0 60-61 31.979076724147106 36.0 31.0 39.0 10.5 41.0 62-63 31.410150859545997 35.0 29.5 39.0 8.0 40.0 64-65 30.92194190260134 35.0 29.0 38.5 6.0 40.0 66-67 30.371486587996152 35.0 28.5 38.0 2.0 40.0 68-69 29.761165362389278 34.0 28.0 37.5 2.0 40.0 70-71 29.11387377036677 34.0 26.5 37.0 2.0 39.0 72-73 28.485216243397318 34.0 26.0 36.0 2.0 39.0 74-75 27.797675891206946 33.0 26.0 36.0 2.0 38.5 76-77 27.0713535366256 32.5 24.5 35.0 2.0 37.5 78-79 26.28971597822578 32.0 22.5 35.0 2.0 37.0 80-81 25.529158115845373 31.5 19.5 35.0 2.0 36.5 82-83 24.801290959690377 31.0 17.0 35.0 2.0 36.0 84-85 24.070895694465634 31.0 9.0 34.0 2.0 35.5 86-87 23.35557718265441 30.0 4.5 34.0 2.0 35.0 88-89 22.588948643634286 29.5 2.0 34.0 2.0 35.0 90-91 21.76938834061151 29.0 2.0 34.0 2.0 35.0 92-93 20.907990576211542 29.0 2.0 34.0 2.0 35.0 94-95 20.00178314555523 27.0 2.0 34.0 2.0 35.0 96-97 18.91826375610604 25.5 2.0 33.5 2.0 35.0 98-99 17.653119601231673 22.5 2.0 33.0 2.0 35.0 100-101 14.765742897127634 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5314228093146802 1101 2 0.4981874363830485 1101 3 0.5537509029905685 1101 4 0.44583369306758414 1101 5 0.5035507009689866 1101 6 0.33440816232805304 1101 7 0.40836178138819434 1101 8 0.3758151572938502 1101 9 0.5468130167427034 1101 10-11 0.5464082187766905 1101 12-13 0.6361950456753718 1101 14-15 0.7047932102701608 1101 16-17 0.815188326226874 1101 18-19 0.9163302789462477 1101 20-21 0.819143487315074 1101 22-23 0.7582395853381385 1101 24-25 0.8109268778098624 1101 26-27 0.8201576898330885 1101 28-29 0.8216195957077588 1101 30-31 0.8631323952136114 1101 32-33 0.9176004536672551 1101 34-35 1.0134825271550127 1101 36-37 1.0670338501144343 1101 38-39 1.0711950302802364 1101 40-41 1.1556954581935273 1101 42-43 1.2165851766295575 1101 44-45 1.3420102414962756 1101 46-47 1.3488717395519743 1101 48-49 1.437420120632808 1101 50-51 1.4445326095023532 1101 52-53 1.6465364234826225 1101 54-55 1.701878905598214 1101 56-57 1.8791749298022538 1101 58-59 1.975660130002865 1101 60-61 1.9464720489176202 1101 62-63 1.9494206950083708 1101 64-65 1.968264743853318 1101 66-67 2.0261279194133017 1101 68-69 2.273420662915722 1101 70-71 2.43528033115318 1101 72-73 2.443406557572949 1101 74-75 2.6303928681289754 1101 76-77 2.9041752632764357 1101 78-79 3.0677202266147034 1101 80-81 3.3835739992519223 1101 82-83 3.570006360743509 1101 84-85 3.7178245530571594 1101 86-87 4.039825278342686 1101 88-89 4.311165593587667 1101 90-91 4.569129814041364 1101 92-93 4.573873491444065 1101 94-95 4.776941297453959 1101 96-97 4.942310908694566 1101 98-99 5.358229319051656 1101 100-101 5.500454987958518 1102 1 0.5638820867817209 1102 2 0.4737206553250424 1102 3 0.4443228908078609 1102 4 0.42071559619257215 1102 5 0.3962044007796308 1102 6 0.5152704635129979 1102 7 0.5624198028473586 1102 8 0.5955252206512824 1102 9 0.627546040045246 1102 10-11 0.60154074390055 1102 12-13 0.6706599173489636 1102 14-15 0.8555487050631427 1102 16-17 0.9254873139311428 1102 18-19 0.9487648599909164 1102 20-21 1.0555143303388874 1102 22-23 0.825050974542485 1102 24-25 0.7288474326067842 1102 26-27 0.7475635383451689 1102 28-29 0.7004276504121023 1102 30-31 0.8006576913372356 1102 32-33 0.8164851542174958 1102 34-35 0.9051509781677112 1102 36-37 1.0514898510391788 1102 38-39 1.0353499924419083 1102 40-41 1.2805441805320115 1102 42-43 1.4022540069432452 1102 44-45 1.5372476314289614 1102 46-47 1.5405098271666375 1102 48-49 1.358193804485758 1102 50-51 1.4043951595834514 1102 52-53 1.4391987847503884 1102 54-55 1.5850978958912734 1102 56-57 1.730427927101104 1102 58-59 1.775837577178443 1102 60-61 1.6790789575405398 1102 62-63 2.1289791410077186 1102 64-65 2.1544562560761165 1102 66-67 2.2295839696020927 1102 68-69 2.325081811257501 1102 70-71 2.2216235959686976 1102 72-73 2.4007665583199618 1102 74-75 2.6055216388814557 1102 76-77 2.8632280154141903 1102 78-79 3.2409163332736277 1102 80-81 3.6159756731992587 1102 82-83 3.861569006744606 1102 84-85 3.876690681382609 1102 86-87 3.95454521543925 1102 88-89 4.225565668546299 1102 90-91 4.469874409565804 1102 92-93 4.434292338903571 1102 94-95 4.687750924744051 1102 96-97 4.9203777165419105 1102 98-99 5.3362672446423325 1102 100-101 5.286264784291925 1103 1 0.5762973613761879 1103 2 0.4884251606510759 1103 3 0.44686763653218975 1103 4 0.4330237686942624 1103 5 0.36574793166022346 1103 6 0.4921293733360983 1103 7 0.5110031481659263 1103 8 0.4079340691201807 1103 9 0.5809823341904305 1103 10-11 0.5201079627040102 1103 12-13 0.6701271609389039 1103 14-15 0.7889051928489152 1103 16-17 0.9580204926294584 1103 18-19 1.0279472732152755 1103 20-21 0.9420023303144021 1103 22-23 0.8402056146641499 1103 24-25 0.8971960101379892 1103 26-27 0.9861366630640305 1103 28-29 1.0284432628371718 1103 30-31 1.0042771300610696 1103 32-33 1.1218350567545912 1103 34-35 1.0520142638763161 1103 36-37 1.1894780453284923 1103 38-39 1.2986110675633142 1103 40-41 1.2527319090802322 1103 42-43 1.2873452426259817 1103 44-45 1.2408875725534116 1103 46-47 1.4661385929527242 1103 48-49 1.4203677933655001 1103 50-51 1.453013389583667 1103 52-53 1.5543021688705139 1103 54-55 1.6779674975632872 1103 56-57 1.7850019514775468 1103 58-59 1.8761862709919157 1103 60-61 1.995053081674925 1103 62-63 2.1157844691838363 1103 64-65 2.2913325776394373 1103 66-67 2.320620595457765 1103 68-69 2.3910221217311722 1103 70-71 2.493186001298099 1103 72-73 2.727907146647052 1103 74-75 2.9324763285760937 1103 76-77 3.147515832398131 1103 78-79 3.0760341914765768 1103 80-81 3.4489225719816616 1103 82-83 3.7486295439075015 1103 84-85 4.019339193734538 1103 86-87 4.155799533948635 1103 88-89 4.453784774947636 1103 90-91 4.97751828592715 1103 92-93 5.077062794638277 1103 94-95 5.190276545094676 1103 96-97 5.271862457553748 1103 98-99 5.4208540294681065 1103 100-101 5.446170503849684 1104 1 0.5097986906182648 1104 2 0.45350158213381064 1104 3 0.43984417350737814 1104 4 0.33016110023937273 1104 5 0.32419392786498236 1104 6 0.39227382407691636 1104 7 0.4050470534689268 1104 8 0.5148884865078713 1104 9 0.6568477042829954 1104 10-11 0.5800684204493294 1104 12-13 0.6077827549414749 1104 14-15 0.7065476023145294 1104 16-17 0.8613965176675862 1104 18-19 0.8226623293659543 1104 20-21 0.8653802972610762 1104 22-23 0.8209576670116405 1104 24-25 0.9501572503248212 1104 26-27 0.9016316169408611 1104 28-29 0.7717090419797117 1104 30-31 0.9544257258113618 1104 32-33 1.014536887486038 1104 34-35 0.9756343448005325 1104 36-37 1.1626214501641883 1104 38-39 1.168902819448732 1104 40-41 1.3360067391064447 1104 42-43 1.3413883870642351 1104 44-45 1.298664916272358 1104 46-47 1.306677069428936 1104 48-49 1.417503194358261 1104 50-51 1.4836613056659544 1104 52-53 1.5565768705717815 1104 54-55 1.6558087879899048 1104 56-57 1.9052650289824058 1104 58-59 1.8803442265142607 1104 60-61 2.041392073040818 1104 62-63 2.1353472672128504 1104 64-65 2.2297644240310674 1104 66-67 2.3472121939210417 1104 68-69 2.619278197009084 1104 70-71 2.792506730774342 1104 72-73 2.8964752559662834 1104 74-75 2.858168119299677 1104 76-77 3.1384052719360724 1104 78-79 3.2412782871093277 1104 80-81 3.475460909912357 1104 82-83 3.7429404900257843 1104 84-85 3.8747068674303087 1104 86-87 4.003584452246525 1104 88-89 4.187702762812414 1104 90-91 4.514730874945489 1104 92-93 4.5554784243901345 1104 94-95 4.776223713826084 1104 96-97 4.934907199807977 1104 98-99 5.200114616560434 1104 100-101 5.145159291882985 1105 1 0.44948043261930337 1105 2 0.4512183699090748 1105 3 0.4043794049842795 1105 4 0.43040342318943203 1105 5 0.3145317840524342 1105 6 0.4517030146755445 1105 7 0.4351027558099645 1105 8 0.4118266767063261 1105 9 0.4292863187634026 1105 10-11 0.4976105123497305 1105 12-13 0.5540254801768185 1105 14-15 0.6755142642434464 1105 16-17 0.733609972947086 1105 18-19 0.8082770348178343 1105 20-21 0.7707817069093252 1105 22-23 0.7217112751003256 1105 24-25 0.7086423606931191 1105 26-27 0.74265590676562 1105 28-29 0.8487338779141496 1105 30-31 0.8307973980744805 1105 32-33 0.8402563584322351 1105 34-35 0.9083130021913703 1105 36-37 1.124032202475739 1105 38-39 0.9947292597865669 1105 40-41 1.0611689539851668 1105 42-43 1.2965131674147798 1105 44-45 1.2924695219524054 1105 46-47 1.2991971435069658 1105 48-49 1.3804897755424719 1105 50-51 1.2374825399838087 1105 52-53 1.3336727515257962 1105 54-55 1.3421038356930737 1105 56-57 1.511703645154867 1105 58-59 1.6206575479524687 1105 60-61 1.8921521638024643 1105 62-63 2.0299831567286475 1105 64-65 2.1689615518579544 1105 66-67 2.2758580576584286 1105 68-69 2.370462791809242 1105 70-71 2.383393858660451 1105 72-73 2.5808679088241213 1105 74-75 2.6729711602549386 1105 76-77 2.8802090265141267 1105 78-79 3.1934830092871316 1105 80-81 3.5847163691055712 1105 82-83 3.7994172619634448 1105 84-85 4.061158059776819 1105 86-87 4.246515483374356 1105 88-89 4.595656526654452 1105 90-91 4.826257046331708 1105 92-93 4.885921310454986 1105 94-95 5.028080053139973 1105 96-97 5.216102763333094 1105 98-99 5.417610356820351 1105 100-101 5.361288065898487 1106 1 0.24740510990360676 1106 2 0.29726795068936696 1106 3 0.19457152172055459 1106 4 0.2269348335979302 1106 5 0.2910718939446113 1106 6 0.08886000960021789 1106 7 0.20231656099927164 1106 8 0.17246377266049961 1106 9 0.20036452122160142 1106 10-11 0.16550991970525075 1106 12-13 0.14695437225425678 1106 14-15 0.45481657004599185 1106 16-17 0.24211040729360178 1106 18-19 0.40140121021850916 1106 20-21 0.4776006333704359 1106 22-23 0.3199951199206126 1106 24-25 0.4685728589739355 1106 26-27 0.5856656111463749 1106 28-29 0.3574071564394856 1106 30-31 0.6304995537387512 1106 32-33 0.6105701392680132 1106 34-35 0.5368197854035017 1106 36-37 0.5691741434021012 1106 38-39 0.5722891424879037 1106 40-41 0.6686412361497176 1106 42-43 0.2804004494726229 1106 44-45 0.5744480487896269 1106 46-47 0.7890574747401118 1106 48-49 0.8411151968648696 1106 50-51 0.14618433034508627 1106 52-53 0.572810636459117 1106 54-55 0.5464035366718534 1106 56-57 0.6198203073650888 1106 58-59 -0.0632714112854913 1106 60-61 1.1774457996217436 1106 62-63 1.6580349588273222 1106 64-65 1.7862045907018427 1106 66-67 1.856910914867644 1106 68-69 1.2592605903251837 1106 70-71 1.675337562456214 1106 72-73 2.0950567447377964 1106 74-75 2.2550726944019814 1106 76-77 2.420264076168973 1106 78-79 2.6348812221897333 1106 80-81 3.066339023665769 1106 82-83 3.218644658683754 1106 84-85 3.4671891996602504 1106 86-87 3.8492556951998758 1106 88-89 3.7245016231208083 1106 90-91 4.054974380900294 1106 92-93 4.283592535249394 1106 94-95 4.168859086882172 1106 96-97 4.542317788082062 1106 98-99 4.52159656882915 1106 100-101 4.076344590574012 1107 1 0.42488101752858043 1107 2 0.33799415691674994 1107 3 0.35293677573480053 1107 4 0.3665874216021692 1107 5 0.35589109581364653 1107 6 0.3640074166906402 1107 7 0.36151462988558336 1107 8 0.35614170337917983 1107 9 0.35280489370741464 1107 10-11 0.42207816735481885 1107 12-13 0.46264947204682016 1107 14-15 0.6129179636430706 1107 16-17 0.6677503761715187 1107 18-19 0.6940847473690539 1107 20-21 0.7981658184617331 1107 22-23 0.7348673297065531 1107 24-25 0.6483596118950743 1107 26-27 0.713773083289098 1107 28-29 0.7808969695009935 1107 30-31 0.7481951767340718 1107 32-33 0.8629751069436864 1107 34-35 1.0364784723095397 1107 36-37 0.9960200653523472 1107 38-39 1.1124370850444265 1107 40-41 1.019970922355661 1107 42-43 1.1760556819892827 1107 44-45 1.3614938999302453 1107 46-47 1.2576512831632058 1107 48-49 1.4238468941891398 1107 50-51 1.3092710945713435 1107 52-53 1.516450011632493 1107 54-55 1.6459757546151508 1107 56-57 1.825791079604521 1107 58-59 1.9354904878164163 1107 60-61 2.0922344498852183 1107 62-63 2.176526959182212 1107 64-65 2.2742584834659283 1107 66-67 2.403556541216762 1107 68-69 2.625960611292019 1107 70-71 2.5454761099037455 1107 72-73 2.841899642061712 1107 74-75 2.893124469629747 1107 76-77 3.0961876285896146 1107 78-79 3.253132841211336 1107 80-81 3.5239398644443654 1107 82-83 3.6662703394294773 1107 84-85 3.89864713061824 1107 86-87 4.182099190001686 1107 88-89 4.386138061149651 1107 90-91 4.644323019279753 1107 92-93 4.8812205420578785 1107 94-95 4.884039131339016 1107 96-97 5.171843091052356 1107 98-99 5.341847052771847 1107 100-101 5.332642672364136 1108 1 0.3724734232834237 1108 2 0.3654645451004157 1108 3 0.3573480421898978 1108 4 0.33860353014092937 1108 5 0.29841599284861786 1108 6 0.3540807853540855 1108 7 0.34184091253248994 1108 8 0.3556699047896572 1108 9 0.3787395330765406 1108 10-11 0.45061126972994003 1108 12-13 0.5025076861788307 1108 14-15 0.5208253601915231 1108 16-17 0.6779596242936776 1108 18-19 0.6665666345560908 1108 20-21 0.6513343012355719 1108 22-23 0.5822963821810774 1108 24-25 0.6749843242254343 1108 26-27 0.7387378483211791 1108 28-29 0.802444124616386 1108 30-31 0.8186854155710108 1108 32-33 0.9263752970273416 1108 34-35 1.0016259194401513 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0.021915303475687153 2311 20-21 0.04396014001449089 2311 22-23 0.01602015162122683 2311 24-25 -0.05198601064539332 2311 26-27 0.08472110759151974 2311 28-29 0.036574125742795616 2311 30-31 0.10409700153692825 2311 32-33 0.22380157045380145 2311 34-35 0.18824876633211574 2311 36-37 0.28069585774304784 2311 38-39 0.3845144246515204 2311 40-41 0.1939944708687804 2311 42-43 0.4789394907086475 2311 44-45 0.49113349740986934 2311 46-47 0.5723010243288158 2311 48-49 0.6310574353114973 2311 50-51 0.6766662859008434 2311 52-53 0.7130821435748231 2311 54-55 0.8148866654412998 2311 56-57 0.8794942378656643 2311 58-59 0.9629270806507009 2311 60-61 0.8790492794533904 2311 62-63 1.066745400521576 2311 64-65 1.1092879443187655 2311 66-67 1.0116095959632858 2311 68-69 1.001577269679487 2311 70-71 1.292451521462855 2311 72-73 1.3981749741092067 2311 74-75 1.4713006395832586 2311 76-77 1.6354688091658325 2311 78-79 1.8768523182186065 2311 80-81 1.855313864441932 2311 82-83 1.9217011409388967 2311 84-85 2.162783262918051 2311 86-87 2.3824777829990644 2311 88-89 2.4518286863773184 2311 90-91 2.6879321537089744 2311 92-93 2.8478932348344266 2311 94-95 3.0339902907254626 2311 96-97 3.1272687720346504 2311 98-99 3.272285517684761 2311 100-101 3.429040246781744 2312 1 -0.22173996810929708 2312 2 -0.21785527047603992 2312 3 -0.19150922369123435 2312 4 -0.1501000896911293 2312 5 -0.22830584280158206 2312 6 -0.08222230408584608 2312 7 -0.09150406178046211 2312 8 -0.1828259384380928 2312 9 -0.167090209990171 2312 10-11 -0.17282924825396861 2312 12-13 -0.17094524581850123 2312 14-15 -0.1219909400240482 2312 16-17 -0.014942590154667812 2312 18-19 0.0778580912841349 2312 20-21 -0.01005357802432627 2312 22-23 -0.05598518599777691 2312 24-25 -0.17133422010313382 2312 26-27 -0.005799076320514018 2312 28-29 -0.014784206915408049 2312 30-31 0.08055344803545239 2312 32-33 0.181607516381753 2312 34-35 0.02391279402066715 2312 36-37 -0.012482239366569559 2312 38-39 0.18445733680972864 2312 40-41 0.2566705152654194 2312 42-43 0.3037991419138635 2312 44-45 0.328980711127528 2312 46-47 0.21667697697040467 2312 48-49 0.3388231088758076 2312 50-51 0.4770902683210565 2312 52-53 0.5686454291214247 2312 54-55 0.5974219465439674 2312 56-57 0.6496492605594995 2312 58-59 0.7598717035600728 2312 60-61 0.8254074331233099 2312 62-63 0.9815997323206318 2312 64-65 1.130010862292508 2312 66-67 1.2422254789438831 2312 68-69 1.378740829800396 2312 70-71 1.4518462396128342 2312 72-73 1.3923595819961214 2312 74-75 1.4345506554409937 2312 76-77 1.6708511030168616 2312 78-79 1.767123394402926 2312 80-81 2.052449910609603 2312 82-83 2.236315518533832 2312 84-85 2.3725033629254 2312 86-87 2.567911528131056 2312 88-89 2.5675534821579653 2312 90-91 2.783809739540139 2312 92-93 2.926839863668622 2312 94-95 3.0785451845477283 2312 96-97 3.236122929869655 2312 98-99 3.255611267567165 2312 100-101 3.134625952965882 2313 1 -0.41428801967374085 2313 2 -0.4729515845887491 2313 3 -0.35014268150502303 2313 4 -0.23055771359138078 2313 5 -0.20060894215386327 2313 6 -0.2870802064847311 2313 7 -0.28957883444540045 2313 8 -0.2097453637181772 2313 9 -0.39502657727080503 2313 10-11 -0.32848097726635217 2313 12-13 -0.3382195458796815 2313 14-15 -0.3163935393446877 2313 16-17 -0.23343479430260317 2313 18-19 -0.18730109600620182 2313 20-21 -0.28055645795343054 2313 22-23 -0.3438847366022415 2313 24-25 -0.2183957429843275 2313 26-27 -0.26559347259031085 2313 28-29 -0.13754355877312463 2313 30-31 -0.11441617029482387 2313 32-33 -0.06758494305660889 2313 34-35 0.025898679203272934 2313 36-37 -0.02400195580432296 2313 38-39 0.08206161556154257 2313 40-41 0.11622017030687459 2313 42-43 0.36181494460179664 2313 44-45 0.33912534053445853 2313 46-47 0.17125796310876495 2313 48-49 0.2863345547479099 2313 50-51 0.3503269710659467 2313 52-53 0.4992644556420913 2313 54-55 0.5414257864494445 2313 56-57 0.7315784965870975 2313 58-59 0.7656109908567359 2313 60-61 0.8953327448518991 2313 62-63 0.9512054035656519 2313 64-65 1.090722510709078 2313 66-67 1.2168683888326974 2313 68-69 1.3393311625218765 2313 70-71 1.5660006164721239 2313 72-73 1.6219379308367614 2313 74-75 1.6700866598294581 2313 76-77 1.705061074112077 2313 78-79 1.8521467081147627 2313 80-81 1.889714927382819 2313 82-83 2.086781265311057 2313 84-85 2.18465128367988 2313 86-87 2.251429472304739 2313 88-89 2.3145986537933005 2313 90-91 2.5453716527413945 2313 92-93 2.6421483220098665 2313 94-95 2.7569203981688446 2313 96-97 2.922681150854107 2313 98-99 2.8638074614190963 2313 100-101 2.649823117343372 2314 1 -0.4657365353606657 2314 2 -0.52523878190906 2314 3 -0.5721759730747387 2314 4 -0.4591048510789122 2314 5 -0.3756850116007939 2314 6 -0.3881298370045556 2314 7 -0.3003883667781082 2314 8 -0.46421620163272337 2314 9 -0.4954331333064985 2314 10-11 -0.40451910148328096 2314 12-13 -0.5522617100874143 2314 14-15 -0.5404186997936904 2314 16-17 -0.47843978036718937 2314 18-19 -0.4369258626839496 2314 20-21 -0.40114187307527516 2314 22-23 -0.5255890757569119 2314 24-25 -0.5155255596505697 2314 26-27 -0.5101605799057651 2314 28-29 -0.38190930578787885 2314 30-31 -0.46656529198037333 2314 32-33 -0.32762355309521496 2314 34-35 -0.45345006405275257 2314 36-37 -0.425785432753365 2314 38-39 -0.31011281999037266 2314 40-41 -0.26359329973838186 2314 42-43 -0.13064970547873855 2314 44-45 -0.22375980099307213 2314 46-47 -0.24746023474850887 2314 48-49 -0.1564580249652039 2314 50-51 -0.17157527649855098 2314 52-53 -0.04831410683713244 2314 54-55 -0.11256817913392325 2314 56-57 -0.07426159137219912 2314 58-59 0.31512900461316207 2314 60-61 0.39893869233201684 2314 62-63 0.44379981917330724 2314 64-65 0.3787617088476125 2314 66-67 0.580374870716664 2314 68-69 0.8199833142005168 2314 70-71 0.9077320593625089 2314 72-73 0.8031794609193526 2314 74-75 0.9709215060285388 2314 76-77 1.1112904586527215 2314 78-79 1.3653655324263063 2314 80-81 1.5937402156796274 2314 82-83 1.68676464509576 2314 84-85 1.9238705171044082 2314 86-87 2.0182267568172527 2314 88-89 2.2325005433839316 2314 90-91 2.2853349604199344 2314 92-93 2.4392890057399548 2314 94-95 2.604860769950278 2314 96-97 2.7765568572132544 2314 98-99 2.8089223966002557 2314 100-101 2.782676808309647 2315 1 -0.5233716999547084 2315 2 -0.5204013740942628 2315 3 -0.49158320227536123 2315 4 -0.40576399237446736 2315 5 -0.33746452083926215 2315 6 -0.2787275875146378 2315 7 -0.32806123896695993 2315 8 -0.41609566841184886 2315 9 -0.4715677806945351 2315 10-11 -0.36800015436083555 2315 12-13 -0.3963863104282268 2315 14-15 -0.4755066568727102 2315 16-17 -0.34772835852086814 2315 18-19 -0.2773707132783798 2315 20-21 -0.2143905394531842 2315 22-23 -0.36582980451869673 2315 24-25 -0.44373765115756214 2315 26-27 -0.4108260195736122 2315 28-29 -0.41020282518238815 2315 30-31 -0.4691292552601709 2315 32-33 -0.33623478373204563 2315 34-35 -0.3900961398412406 2315 36-37 -0.11389131536612496 2315 38-39 -0.16327639671924032 2315 40-41 -0.12035375570605567 2315 42-43 0.035377607415213674 2315 44-45 -0.08804930883522388 2315 46-47 -0.037346435179365756 2315 48-49 -0.03227552747286211 2315 50-51 0.21854099511581637 2315 52-53 0.24755985732604557 2315 54-55 0.2381434243756715 2315 56-57 0.517287112905656 2315 58-59 0.722393041840597 2315 60-61 0.8579888712881498 2315 62-63 0.7590738706773408 2315 64-65 0.8058372650934125 2315 66-67 1.0579467951219463 2315 68-69 1.1777043948333592 2315 70-71 1.2815515509048367 2315 72-73 1.41240375164897 2315 74-75 1.5683124297043207 2315 76-77 1.5983032270869124 2315 78-79 1.8002663585311005 2315 80-81 1.9219709543865875 2315 82-83 2.1058950515370434 2315 84-85 2.4481277280674654 2315 86-87 2.488606970909192 2315 88-89 2.5999950899239224 2315 90-91 2.7008152175411375 2315 92-93 2.918106231834493 2315 94-95 3.094307737099861 2315 96-97 3.140698345113769 2315 98-99 3.2404023787331404 2315 100-101 3.2589254048718654 2316 1 -0.9982402089450773 2316 2 -0.8737248122236778 2316 3 -0.758151183928419 2316 4 -0.6182661667253626 2316 5 -0.6192300708813789 2316 6 -0.4544506164083302 2316 7 -0.6202974329831434 2316 8 -0.6023940534413299 2316 9 -0.6271540314727275 2316 10-11 -0.6099378901062451 2316 12-13 -0.6267207370404506 2316 14-15 -1.1771797054500013 2316 16-17 -0.9214251212557016 2316 18-19 -0.8152481920384034 2316 20-21 -0.6070828894000826 2316 22-23 -0.8195773555129477 2316 24-25 -0.9450228069784146 2316 26-27 -0.7059486644082682 2316 28-29 -0.8508373236626312 2316 30-31 -0.6687069162079098 2316 32-33 -0.5382428540070734 2316 34-35 -0.6237948022721227 2316 36-37 -0.5170757666972818 2316 38-39 -0.5635526537229367 2316 40-41 -0.6134058462872289 2316 42-43 -0.46841692100240806 2316 44-45 -0.3778728316498885 2316 46-47 -0.22118894808814815 2316 48-49 -0.19816905980889743 2316 50-51 -0.12028524449307554 2316 52-53 -0.022061942944944235 2316 54-55 -0.0661704305882651 2316 56-57 0.18498049280824347 2316 58-59 0.45365471467173535 2316 60-61 0.2837153333674607 2316 62-63 0.549398638593992 2316 64-65 0.6492387967519591 2316 66-67 0.7964856770936031 2316 68-69 0.8992850563853416 2316 70-71 0.7412742753894008 2316 72-73 0.9878743269593322 2316 74-75 1.2425277179088141 2316 76-77 1.1530925782606047 2316 78-79 1.3931780890397611 2316 80-81 1.8416942052929457 2316 82-83 1.9646174601685296 2316 84-85 1.8839139962404623 2316 86-87 2.0532947684519485 2316 88-89 2.137506934414347 2316 90-91 2.379122576917851 2316 92-93 2.461603263273318 2316 94-95 2.5880358791513487 2316 96-97 2.4738563952474344 2316 98-99 2.625366341727972 2316 100-101 2.724349220193945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11813.0 3 4182.0 4 4738.0 5 5591.0 6 6487.0 7 8181.0 8 10611.0 9 13850.0 10 17559.0 11 21759.0 12 26800.0 13 31758.0 14 36117.0 15 42630.0 16 48883.0 17 56380.0 18 55823.0 19 55508.0 20 59977.0 21 65778.0 22 72362.0 23 81662.0 24 91926.0 25 103272.0 26 116443.0 27 131700.0 28 148397.0 29 166334.0 30 189001.0 31 215775.0 32 250612.0 33 291667.0 34 346261.0 35 414555.0 36 489340.0 37 507212.0 38 287002.0 39 22339.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78717976944623 18.209166330298796 14.036559017828054 23.96709488242692 2 15.501455714324685 19.787956084823776 39.919064634514456 24.79152356633708 3 19.484943470920108 23.493393332913385 29.105001335262738 27.916661860903773 4 12.931865124994676 15.93023348664878 36.265158532430476 34.87274285592607 5 14.098298758104729 37.06917951854457 33.81005020956331 15.022471513787385 6 33.33039027014436 35.29151299698854 17.012850020242805 14.3652467126243 7 29.7221317243464 31.922483154273213 20.105601965133342 18.24978315624704 8 26.77032283619816 34.83596040791495 19.354757865643382 19.0389588902435 9 26.5733899688883 16.92219723536811 19.77781094900338 36.726601846740216 10-11 25.353199096016127 26.11826974470662 27.081380726691258 21.447150432586 12-13 26.200344611077192 23.361605872538256 27.404198985506508 23.033850530878045 14-15 22.821891204421647 25.13186974887059 25.584322511229818 26.461916535477947 16-17 22.75470042861806 28.369135773996224 26.2091362529982 22.667027544387516 18-19 22.718725019729185 27.071042906104843 28.034022123961023 22.176209950204953 20-21 23.229040092826022 26.210298671475798 28.202968538583452 22.357692697114732 22-23 22.905990203916883 26.047745093364412 28.205866339021657 22.84039836369705 24-25 22.768105559553987 26.29741985080128 28.26463845668642 22.669836132958313 26-27 22.39834955303537 26.57629296897571 28.207287159871747 22.818070318117176 28-29 22.425524458851175 27.06449427162405 28.120043604635043 22.389937664889732 30-31 22.562367291689746 26.41924353809573 28.035136852230686 22.983252317983833 32-33 22.325837117148605 26.53599095299804 28.118284804465482 23.01988712538787 34-35 23.088343119073166 26.549986670930785 27.85001448233311 22.51165572766294 36-37 23.19174455025568 26.156244448569556 27.87581562364645 22.776195377528317 38-39 22.58608818099668 26.612499237716143 27.865671724535563 22.935740856751618 40-41 22.927036418590934 26.41760412993873 27.961042330541456 22.69431712092888 42-43 22.70976460514001 26.389402469095284 27.697872397515848 23.20296052824886 44-45 22.618006922746115 26.52425527706672 27.919336847885507 22.938400952301656 46-47 22.982412607432064 26.92831106303583 27.29221300187999 22.79706332765211 48-49 22.902038899365103 26.66553650718187 27.466356874355828 22.966067719097197 50-51 22.876107053155735 26.934386400007536 27.442490331213786 22.74701621562294 52-53 23.068915365164063 26.827987691115148 27.22505421472434 22.878042728996444 54-55 22.560668771396195 26.73458090867597 27.559591720756632 23.145158599171197 56-57 22.792152101263667 26.947784147751864 27.352569196602783 22.90749455438168 58-59 22.96073957846103 26.531746465528794 27.35925668759325 23.148257268416927 60-61 22.54208688884627 26.80984431315639 27.754209243206507 22.893859554790836 62-63 22.482374272396548 26.83354406418043 27.810961958139487 22.873119705283536 64-65 22.281562697896025 26.842251256334894 27.615110076754835 23.26107596901425 66-67 22.38651989923227 26.787746991579482 27.436591138168946 23.3891419710193 68-69 22.5699949223609 26.55955582853101 27.672855917001666 23.197593332106422 70-71 22.67626268427427 26.495226530794163 27.46737157400973 23.361139210921834 72-73 22.576655909109654 26.520733760885985 27.64594214512812 23.25666818487624 74-75 22.46729335032882 27.087584006872106 27.208741714893787 23.236380927905287 76-77 22.619310401561194 26.607283045212565 27.309207739309567 23.464198813916674 78-79 22.8148608849946 26.691367181242242 27.35751611270663 23.136255821056523 80-81 22.903878588334734 26.9194077089281 27.139896564917105 23.03681713782006 82-83 22.881967315260688 26.77758928621914 27.21638004333027 23.124063355189904 84-85 22.82248900526927 26.728608915737183 27.25429395924494 23.194608119748608 86-87 22.875722509710418 26.774866534071904 27.09146876763697 23.257942188580703 88-89 22.82928084080881 26.7599999334733 26.977076561700468 23.433642664017427 90-91 22.85408361856472 26.634695469131746 27.05642430675938 23.45479660554415 92-93 22.971629927110726 26.332494921521683 27.243339216185845 23.452535935181743 94-95 22.81190931882898 26.228514271261805 27.420778937673962 23.53879747223525 96-97 22.803986722751 26.114051866069516 27.43084433515463 23.651117076024857 98-99 22.909436706432118 26.225462668486877 26.992669525740475 23.872431099340528 100-101 23.277068768106083 25.63803657426077 27.342707752361044 23.7421869052721 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 732.0 1 497.5 2 399.0 3 532.5 4 460.0 5 485.0 6 861.5 7 1797.5 8 2919.0 9 3370.5 10 2986.5 11 2620.5 12 2727.5 13 2898.5 14 3149.5 15 3430.5 16 3596.5 17 3825.5 18 4213.0 19 4676.0 20 5263.0 21 6118.5 22 7398.0 23 9234.0 24 11493.5 25 14615.0 26 19104.0 27 24730.0 28 30078.0 29 35898.0 30 43400.0 31 52392.5 32 64169.5 33 71525.5 34 75760.5 35 83593.0 36 94279.5 37 104829.0 38 112580.5 39 119356.0 40 126398.0 41 136828.0 42 146685.0 43 154017.0 44 160183.5 45 167878.0 46 175210.5 47 183670.0 48 191697.0 49 189728.0 50 180578.0 51 168753.0 52 161206.0 53 160518.5 54 161656.0 55 153664.0 56 136263.5 57 121425.0 58 110331.0 59 96379.0 60 79900.0 61 66984.5 62 56528.5 63 44725.5 64 34694.0 65 27354.0 66 20398.0 67 15991.0 68 12657.5 69 9072.0 70 6813.0 71 5238.0 72 4125.0 73 3261.0 74 2415.5 75 1839.0 76 1328.0 77 829.5 78 518.5 79 351.0 80 211.0 81 116.0 82 69.5 83 44.5 84 32.5 85 22.5 86 16.5 87 11.5 88 7.5 89 5.5 90 6.0 91 4.0 92 4.0 93 4.5 94 2.5 95 2.5 96 2.0 97 0.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30425994969722553 2 0.03336815742143296 3 0.04017481810474187 4 0.0429240882178699 5 0.057224727112769734 6 0.0557170628571834 7 0.02840173399126619 8 0.031195347170734994 9 0.037580748723806554 10-11 0.030108942045386014 12-13 0.03186049316584661 14-15 0.026572582504709237 16-17 0.02022043825139326 18-19 0.02448845838669282 20-21 0.036328057099679664 22-23 0.02977636904783021 24-25 0.05040698066287563 26-27 0.04286865938494393 28-29 0.035319252340427036 30-31 0.03490907897677487 32-33 0.030840602640008794 34-35 0.02984288364734137 36-37 0.029155566119059357 38-39 0.018025456467524913 40-41 0.017249452806561354 42-43 0.01823608603264359 44-45 0.008258896105969287 46-47 0.015387044020248817 48-49 0.013302919902232407 50-51 0.006329972720145588 52-53 0.004711450798707311 54-55 0.006352144253315974 56-57 0.005321167960892962 58-59 0.006950775648916433 60-61 0.006052828555515746 62-63 0.0042236770689587895 64-65 0.007627007410613247 66-67 0.0025275547814241576 68-69 0.007050547548183175 70-71 0.012637773907120788 72-73 0.013081204570528535 74-75 0.010276505624474535 76-77 0.008924042101080906 78-79 0.005775684390885903 80-81 0.006862089516234883 82-83 0.011252053083971578 84-85 0.003835675238477011 86-87 0.0012083485577861104 88-89 0.018191742966302816 90-91 0.027259900032991242 92-93 0.012659945440291176 94-95 0.020586268548704652 96-97 0.017060994774613064 98-99 0.01224977207663901 100-101 0.1009026474584328 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4510288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.0455194409893 #Duplication Level Percentage of deduplicated Percentage of total 1 72.06572596435021 33.183037859204944 2 14.255990404394463 13.128489666322043 3 5.329338061587588 7.361764179673067 4 2.654375882814511 4.888884652633148 5 1.462036214192315 3.366010846201132 6 0.9230980199363544 2.5502716693750886 7 0.6397813109419624 2.062134395367183 8 0.4380474107777494 1.613609645523351 9 0.3218807409465136 1.333904932343946 >10 1.6917223750021617 14.086517003964257 >50 0.12743342882116482 4.030620374219957 >100 0.08174169358976827 7.154540525469766 >500 0.005287394277427845 1.6663217071195107 >1k 0.0033470655806437295 2.873886966048228 >5k 1.4552459046276753E-4 0.46040890073718477 >10k+ 4.850819682092251E-5 0.23959667579720473 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10727 0.23783403631874506 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8103 0.17965593327964866 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6853 0.15194151681666448 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5657 0.12542436314488123 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.434306634077469E-5 0.0 10-11 0.0 0.0 0.0 6.651459951116203E-5 0.0 12-13 0.0 0.0 0.0 9.977189926674305E-5 4.434306634077469E-5 14-15 0.0 0.0 0.0 1.1085766585193672E-4 4.434306634077469E-5 16-17 0.0 0.0 0.0 1.1085766585193672E-4 4.434306634077469E-5 18-19 0.0 0.0 0.0 1.3302919902232407E-4 4.434306634077469E-5 20-21 0.0 0.0 0.0 1.8845803194829244E-4 4.434306634077469E-5 22-23 0.0 0.0 0.0 3.3257299755581015E-4 4.434306634077469E-5 24-25 0.0 0.0 0.0 4.766879631633279E-4 4.434306634077469E-5 26-27 0.0 0.0 0.0 8.646897936451065E-4 4.434306634077469E-5 28-29 0.0 0.0 0.0 0.003281386909217327 4.434306634077469E-5 30-31 0.0 0.0 0.0 0.012072399811275909 4.434306634077469E-5 32-33 0.0 0.0 0.0 0.031051232205127476 4.434306634077469E-5 34-35 0.0 0.0 0.0 0.05945296619639366 4.434306634077469E-5 36-37 0.0 0.0 0.0 0.10758736470930459 4.434306634077469E-5 38-39 0.0 0.0 0.0 0.18226108842716918 4.434306634077469E-5 40-41 0.0 0.0 0.0 0.26608056957781856 4.434306634077469E-5 42-43 0.0 0.0 0.0 0.3533033810701224 4.434306634077469E-5 44-45 0.0 0.0 0.0 0.46352915822670304 4.434306634077469E-5 46-47 0.0 0.0 0.0 0.5952502367919743 4.434306634077469E-5 48-49 0.0 0.0 0.0 0.7325807132493535 4.434306634077469E-5 50-51 0.0 0.0 0.0 0.8814071296555785 4.434306634077469E-5 52-53 0.0 0.0 0.0 1.0291471409364545 4.434306634077469E-5 54-55 0.0 0.0 0.0 1.190245500952489 4.434306634077469E-5 56-57 0.0 0.0 0.0 1.3834482409992446 4.434306634077469E-5 58-59 0.0 0.0 0.0 1.5907188188426105 4.434306634077469E-5 60-61 0.0 0.0 0.0 1.8155381651903382 4.434306634077469E-5 62-63 0.0 0.0 0.0 2.040945057167081 4.434306634077469E-5 64-65 0.0 0.0 0.0 2.2762737102375725 4.434306634077469E-5 66-67 0.0 0.0 0.0 2.5485179660367585 6.651459951116203E-5 68-69 0.0 0.0 0.0 2.847223946674802 6.651459951116203E-5 70-71 0.0 0.0 0.0 3.16707048419081 8.868613268154938E-5 72-73 0.0 0.0 0.0 3.4925042480657553 9.977189926674305E-5 74-75 0.0 0.0 0.0 3.8221506032430748 1.3302919902232407E-4 76-77 0.0 0.0 0.0 4.181019039138964 1.4411496560751776E-4 78-79 0.0 1.1085766585193672E-5 0.0 4.581813844260056 1.5520073219271141E-4 80-81 0.0 2.2171533170387345E-5 0.0 5.007019507401745 1.5520073219271141E-4 82-83 0.0 2.2171533170387345E-5 0.0 5.4373135374060375 1.7737226536309876E-4 84-85 0.0 2.2171533170387345E-5 0.0 5.882384894268393 1.7737226536309876E-4 86-87 0.0 2.2171533170387345E-5 0.0 6.335537774971354 1.8845803194829244E-4 88-89 0.0 2.2171533170387345E-5 0.0 6.821027836803326 2.1062956511867976E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19380 0.0 27.025246 1 GTACATG 16090 0.0 26.449726 1 TACATGG 16035 0.0 25.8732 2 ACATGGG 16410 0.0 24.094595 3 GGTATCA 14235 0.0 23.73638 1 ATCAACG 22730 0.0 22.51755 3 TATCAAC 23025 0.0 22.435452 2 TCAACGC 22945 0.0 22.244421 4 CAACGCA 23020 0.0 22.151302 5 AACGCAG 23815 0.0 21.528946 6 GAGTACT 11350 0.0 20.591074 12-13 CATGGGG 9160 0.0 20.493046 4 GGACCGA 1405 0.0 19.615686 6 AGAGTAC 19080 0.0 19.60579 10-11 GTACTTT 11885 0.0 19.485615 14-15 AGTACTT 11990 0.0 19.333496 12-13 ACGCAGA 26640 0.0 19.06334 7 CGCAGAG 26515 0.0 19.027792 8 TAAGGTG 2280 0.0 18.759123 5 GAGTACA 12290 0.0 18.729277 1 >>END_MODULE