FastQCFastQC Report
Thu 26 May 2016
SRR938549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510288
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106180.23541733920317284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85600.1897883239385157No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68960.15289489274299115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59070.13096724643747806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194200.026.6461091
GTACATG162000.025.8064331
TACATGG159550.025.5767062
ACATGGG161000.024.3319633
GGTATCA142350.024.089731
TAAGGTG21000.022.6389625
ATCAACG227750.022.4401573
TCAACGC230450.022.2391324
CAACGCA231800.022.0480825
TATCAAC233500.021.9576592
AACGCAG239200.021.3846246
GTAAGGT19800.021.1296984
CATGGGG86100.020.7063664
AGTACTT123750.020.18773712-13
AGAGTAC197800.020.09769610-11
GAGTACT117700.020.09555212-13
GTACTTT122700.019.08312214-15
ACGCAGA272000.018.7185847
CGCAGAG270650.018.6891128
TACTTTT141750.017.4399314-15
Produced by FastQC (version 0.11.5)