##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938549_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4510288 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.810490593948767 31.0 31.0 34.0 30.0 34.0 2 32.04541018223227 33.0 31.0 34.0 30.0 34.0 3 32.1204914187298 33.0 31.0 34.0 30.0 34.0 4 35.78448759813121 37.0 35.0 37.0 35.0 37.0 5 35.65219892831677 37.0 35.0 37.0 33.0 37.0 6 35.68890434491101 37.0 35.0 37.0 33.0 37.0 7 35.619695238973655 37.0 35.0 37.0 33.0 37.0 8 35.60916730816302 37.0 35.0 37.0 33.0 37.0 9 37.17881452359583 39.0 37.0 39.0 33.0 39.0 10-11 37.11287771423909 39.0 37.0 39.0 33.0 39.0 12-13 37.00767456534926 39.0 37.0 39.0 33.0 39.0 14-15 38.07254448052985 40.0 37.0 41.0 33.0 41.0 16-17 37.99997217472587 40.0 37.0 41.0 33.0 41.0 18-19 37.99229594651162 40.0 37.0 41.0 33.0 41.0 20-21 37.977016323569586 40.0 37.0 41.0 33.0 41.0 22-23 38.00988451291802 40.0 37.0 41.0 33.0 41.0 24-25 37.937239373627584 40.0 37.0 41.0 33.0 41.0 26-27 37.77134508927146 40.0 37.0 41.0 32.0 41.0 28-29 37.65170893743371 40.0 37.0 41.0 32.0 41.0 30-31 37.43059068511811 39.0 36.5 41.0 32.0 41.0 32-33 37.23161370183013 39.0 36.0 41.0 31.0 41.0 34-35 37.0728364796217 39.0 36.0 41.0 31.0 41.0 36-37 37.096170798849215 39.0 36.0 41.0 31.0 41.0 38-39 36.96523570113483 39.0 36.0 41.0 31.0 41.0 40-41 36.81111649633017 39.0 35.0 41.0 30.0 41.0 42-43 36.59940074780147 39.0 35.0 40.5 30.0 41.0 44-45 36.34519780111603 39.0 35.0 40.0 30.0 41.0 46-47 36.02053981918671 38.5 35.0 40.0 29.5 41.0 48-49 35.660715568495846 38.0 35.0 40.0 28.0 41.0 50-51 35.308334523205616 38.0 34.0 40.0 27.0 41.0 52-53 34.80990415689641 38.0 33.5 40.0 26.0 41.0 54-55 34.504119914293724 38.0 33.0 40.0 25.5 41.0 56-57 34.14775586392709 37.0 33.0 40.0 24.5 41.0 58-59 33.700720441798836 37.0 32.5 39.5 23.0 41.0 60-61 33.29779661520506 36.0 32.0 39.0 22.5 40.5 62-63 32.834046517650314 36.0 31.0 39.0 22.0 40.0 64-65 32.34853417342751 35.0 31.0 39.0 20.0 40.0 66-67 31.856138676731952 35.0 30.5 38.0 18.5 40.0 68-69 31.297340990198407 35.0 30.0 38.0 17.5 40.0 70-71 30.727443236440777 34.0 29.0 37.0 13.0 39.0 72-73 30.149512403642518 34.0 29.0 36.5 9.0 39.0 74-75 29.537328214960993 34.0 28.0 36.0 7.0 38.5 76-77 28.237395815965634 31.5 26.5 34.5 7.0 36.5 78-79 28.876126092169724 33.0 28.0 35.0 2.0 37.0 80-81 28.573562486475367 33.0 27.0 35.0 2.0 37.0 82-83 28.089826525490167 33.0 26.5 35.0 2.0 36.0 84-85 27.50062157893243 33.0 26.0 35.0 2.0 36.0 86-87 26.871888225319537 32.0 25.0 35.0 2.0 35.5 88-89 26.134274795755836 32.0 24.0 34.0 2.0 35.0 90-91 25.598363341764426 31.5 22.0 34.0 2.0 35.0 92-93 24.826060774833003 31.0 19.0 34.0 2.0 35.0 94-95 24.045091244727608 31.0 12.0 34.0 2.0 35.0 96-97 23.067910186666573 30.5 2.0 34.0 2.0 35.0 98-99 21.89930709524536 30.0 2.0 34.0 2.0 35.0 100-101 18.709645703334246 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30315951187604995 1101 2 0.3581884066170957 1101 3 0.30756701257454466 1101 4 0.24112716014698066 1101 5 0.19285419122500969 1101 6 0.22368028429946918 1101 7 0.26819203460632934 1101 8 0.2571246038274566 1101 9 0.3666687295331741 1101 10-11 0.29967712941747493 1101 12-13 0.3683610023804036 1101 14-15 0.46324226757185016 1101 16-17 0.4644730523085556 1101 18-19 0.5653397011021895 1101 20-21 0.47352844835238983 1101 22-23 0.49559972465595337 1101 24-25 0.5366247206225339 1101 26-27 0.5744681064487978 1101 28-29 0.5275462810194043 1101 30-31 0.6121447279303212 1101 32-33 0.49622222052069986 1101 34-35 0.3431607001860115 1101 36-37 0.5215599313645569 1101 38-39 0.6407163053226128 1101 40-41 0.7228864583713204 1101 42-43 0.6518590982675292 1101 44-45 0.7293763680842247 1101 46-47 0.8036612894963469 1101 48-49 0.9367495138788087 1101 50-51 0.87125818853211 1101 52-53 1.0241278915835537 1101 54-55 1.1124067650912934 1101 56-57 1.0947192370687233 1101 58-59 1.1328625643356744 1101 60-61 1.199484785976118 1101 62-63 1.343419252584063 1101 64-65 1.2614410638968678 1101 66-67 1.4523809816366118 1101 68-69 1.422215490025721 1101 70-71 1.546015725490122 1101 72-73 1.5348702757223407 1101 74-75 1.6172349163823618 1101 76-77 1.3403401098746812 1101 78-79 1.6588126605250757 1101 80-81 2.010616757017978 1101 82-83 2.0337816568856333 1101 84-85 2.2115450322239525 1101 86-87 2.3657698605644555 1101 88-89 2.5694841826506725 1101 90-91 2.797737399317345 1101 92-93 3.0821272774906596 1101 94-95 2.9893693932447825 1101 96-97 3.43311822839501 1101 98-99 3.8021959741621814 1101 100-101 4.31774856333443 1102 1 0.34420992445441456 1102 2 0.3489726162333753 1102 3 0.29732891265996386 1102 4 0.2523909826573032 1102 5 0.18102096645504417 1102 6 0.2466748108381367 1102 7 0.3162041335993777 1102 8 0.33207331930760375 1102 9 0.3799452943670758 1102 10-11 0.4439307999916764 1102 12-13 0.38755588613174297 1102 14-15 0.5015258114794889 1102 16-17 0.48148750277452024 1102 18-19 0.5238602083093937 1102 20-21 0.4877443466018363 1102 22-23 0.5439307842226313 1102 24-25 0.44232634211814315 1102 26-27 0.4112684804691895 1102 28-29 0.33993876545051194 1102 30-31 0.3129543326813078 1102 32-33 0.16387106637348126 1102 34-35 0.380575941066283 1102 36-37 0.481675724420775 1102 38-39 0.5072673678322559 1102 40-41 0.5246555851386674 1102 42-43 0.4495139041043785 1102 44-45 0.5577235463328307 1102 46-47 0.6591066574360127 1102 48-49 0.6382783415586317 1102 50-51 0.6875722523235765 1102 52-53 0.7632436437473871 1102 54-55 0.683908317230852 1102 56-57 0.7518860776621707 1102 58-59 0.8144898750369549 1102 60-61 0.9056937949862842 1102 62-63 1.081733063293548 1102 64-65 0.9613497161129629 1102 66-67 0.9960860503277651 1102 68-69 1.183557829127885 1102 70-71 1.338441683716141 1102 72-73 1.4106266640299516 1102 74-75 1.4223267584166983 1102 76-77 1.4530599909454693 1102 78-79 1.6588433459103626 1102 80-81 1.886147722049195 1102 82-83 1.8874587030260876 1102 84-85 2.024726321029455 1102 86-87 2.124834687224226 1102 88-89 2.391600263563493 1102 90-91 2.564126148052342 1102 92-93 2.7789220553725826 1102 94-95 2.9485016440355025 1102 96-97 3.307005094309538 1102 98-99 3.685930498247309 1102 100-101 4.362752013427855 1103 1 0.2911004446951786 1103 2 0.29945013856416125 1103 3 0.3645262956968267 1103 4 0.22828597364542702 1103 5 0.20863892410994112 1103 6 0.2891521128446186 1103 7 0.3193694594498453 1103 8 0.30534583905264157 1103 9 0.4110250976664389 1103 10-11 0.4087893793283115 1103 12-13 0.3892560553530515 1103 14-15 0.5454365984962593 1103 16-17 0.5641571601396294 1103 18-19 0.49226570295274996 1103 20-21 0.5161173740107969 1103 22-23 0.5037944279582263 1103 24-25 0.4489626937924527 1103 26-27 0.45462646768425685 1103 28-29 0.4459530069687929 1103 30-31 0.5983997702851624 1103 32-33 0.5627682208238767 1103 34-35 0.5890454215599306 1103 36-37 0.70706886560275 1103 38-39 0.7306633609899649 1103 40-41 0.787059996520874 1103 42-43 0.856132354428901 1103 44-45 0.9716523265011929 1103 46-47 0.9205370517859208 1103 48-49 1.052232979232258 1103 50-51 1.0804505751660045 1103 52-53 1.2940246322079005 1103 54-55 1.311234498184426 1103 56-57 1.3475817975565363 1103 58-59 1.4851570598737496 1103 60-61 1.4995762764499787 1103 62-63 1.6530349045315234 1103 64-65 1.4811377457710648 1103 66-67 1.6094921722833853 1103 68-69 1.7359826205083095 1103 70-71 1.7177434392724464 1103 72-73 1.7730376545407083 1103 74-75 1.9224110630640148 1103 76-77 1.736618595680607 1103 78-79 1.977974992742741 1103 80-81 2.2111713610111003 1103 82-83 2.5188803150043846 1103 84-85 2.4949900369824647 1103 86-87 2.6641278440125085 1103 88-89 2.7958058567407846 1103 90-91 3.080269952502281 1103 92-93 3.2743194194370737 1103 94-95 3.258626769880461 1103 96-97 3.5995090188362227 1103 98-99 3.956217623657743 1103 100-101 4.259906769831797 1104 1 0.260119849017574 1104 2 0.26694974393655 1104 3 0.18521704732899735 1104 4 0.09702067827659278 1104 5 0.09787325569025285 1104 6 0.12138257082841619 1104 7 0.1355706548024429 1104 8 0.16819406243344304 1104 9 0.21676099583320507 1104 10-11 0.2709627610995824 1104 12-13 0.3245449057318339 1104 14-15 0.3934699334573182 1104 16-17 0.3554747848334401 1104 18-19 0.4327819212959554 1104 20-21 0.47173414574114503 1104 22-23 0.38045376609674264 1104 24-25 0.42225367232130395 1104 26-27 0.3520767190535352 1104 28-29 0.4686283664206101 1104 30-31 0.4573030802739524 1104 32-33 0.4408779148086168 1104 34-35 0.41192899676119055 1104 36-37 0.4833705973082161 1104 38-39 0.4225617412565441 1104 40-41 0.5431616775777215 1104 42-43 0.615802320825928 1104 44-45 0.5442631827920081 1104 46-47 0.5047830632079737 1104 48-49 0.5254728549046774 1104 50-51 0.5571490921611186 1104 52-53 0.7345116651419872 1104 54-55 0.6977534510982579 1104 56-57 0.8421579107186972 1104 58-59 1.066231514464313 1104 60-61 1.0032433038649557 1104 62-63 1.0102690816676514 1104 64-65 0.9267521554138298 1104 66-67 1.088420988275633 1104 68-69 1.23757379311445 1104 70-71 1.258242793673027 1104 72-73 1.3988735553194012 1104 74-75 1.5459052038376164 1104 76-77 1.4881364072973078 1104 78-79 1.7567269928517604 1104 80-81 1.969571875673644 1104 82-83 2.1212022584573695 1104 84-85 2.1691875234032985 1104 86-87 2.283724369926766 1104 88-89 2.4088625229948413 1104 90-91 2.625652922986408 1104 92-93 2.894820295567161 1104 94-95 3.126092420243804 1104 96-97 3.4853463732072782 1104 98-99 3.9213291357685485 1104 100-101 4.189208068866407 1105 1 0.22996283508268434 1105 2 0.21894401718765977 1105 3 0.20078593202159567 1105 4 0.16400773492733123 1105 5 0.12307615383635806 1105 6 0.18560365176178806 1105 7 0.16357886886728323 1105 8 0.22405344701733299 1105 9 0.26032878830645245 1105 10-11 0.28846708852172753 1105 12-13 0.33105031564404186 1105 14-15 0.40571467688729257 1105 16-17 0.3709448960096893 1105 18-19 0.36742758875534776 1105 20-21 0.3290374531945517 1105 22-23 0.28996638541811137 1105 24-25 0.28107776409142105 1105 26-27 0.43911855376207853 1105 28-29 0.5045478738305249 1105 30-31 0.5251375375526024 1105 32-33 0.521561548100415 1105 34-35 0.448527598534298 1105 36-37 0.596228751770532 1105 38-39 0.5943306311098979 1105 40-41 0.5021635299512823 1105 42-43 0.6473414765936738 1105 44-45 0.5566859617838347 1105 46-47 0.5922934904764077 1105 48-49 0.7041398692879639 1105 50-51 0.8187241532653289 1105 52-53 0.8460485387550349 1105 54-55 0.8849205399318123 1105 56-57 0.9759082501865706 1105 58-59 1.0205245985415203 1105 60-61 1.041068643100168 1105 62-63 1.1838312290335082 1105 64-65 1.2598455875460601 1105 66-67 1.2346679753661185 1105 68-69 1.3073204274121721 1105 70-71 1.2970773359772778 1105 72-73 1.4208680062359456 1105 74-75 1.537295093152057 1105 76-77 1.4944872588646128 1105 78-79 1.7565248455535887 1105 80-81 1.973051262677167 1105 82-83 1.9997542979295524 1105 84-85 2.1883534984438455 1105 86-87 2.3812985388967363 1105 88-89 2.5554933414317773 1105 90-91 2.576783562488874 1105 92-93 2.7666906344935676 1105 94-95 2.9045915936765603 1105 96-97 3.3113225072414565 1105 98-99 3.7447331397550236 1105 100-101 4.44720602698305 1106 1 0.1666047339552641 1106 2 0.1694961456738895 1106 3 0.14315514790060035 1106 4 0.1507840175990367 1106 5 0.057741838829684866 1106 6 0.0821521347500962 1106 7 0.08386765281002084 1106 8 0.07148374130592572 1106 9 0.0696249335438921 1106 10-11 0.20866227141802085 1106 12-13 0.11701995030338708 1106 14-15 0.07734902490931717 1106 16-17 0.03902209043248206 1106 18-19 0.13573149699524834 1106 20-21 0.3730705080941945 1106 22-23 0.25517056907889213 1106 24-25 0.13985394884686286 1106 26-27 0.15394958857382335 1106 28-29 0.31660983976319557 1106 30-31 0.3033479357579765 1106 32-33 0.3275493317908271 1106 34-35 0.2711261550225643 1106 36-37 0.3399988293126839 1106 38-39 0.2852592696093197 1106 40-41 0.30147323596397513 1106 42-43 0.48778966633530985 1106 44-45 0.1744229756389828 1106 46-47 0.0412474968208727 1106 48-49 0.4965277269177193 1106 50-51 0.29303925722604873 1106 52-53 0.5482271129184468 1106 54-55 0.6107078400665102 1106 56-57 0.5737030628840429 1106 58-59 0.9410772385774706 1106 60-61 0.7538217817558746 1106 62-63 0.9940490268213509 1106 64-65 0.5811884934497868 1106 66-67 0.7593987370165856 1106 68-69 0.6894858111246833 1106 70-71 0.7182673732482279 1106 72-73 1.151625419940956 1106 74-75 1.484306858168086 1106 76-77 1.457375660055174 1106 78-79 1.7186652229580268 1106 80-81 1.649909223834534 1106 82-83 1.9922180406658576 1106 84-85 1.9379538306059487 1106 86-87 2.179064259165031 1106 88-89 2.579913014943795 1106 90-91 2.5196319247137815 1106 92-93 2.237337252176996 1106 94-95 2.6422625391585406 1106 96-97 3.2402902971834493 1106 98-99 3.7017715147718135 1106 100-101 4.103901946134343 1107 1 0.18408654784333223 1107 2 0.1584736864332399 1107 3 0.13548748184616244 1107 4 0.15869481554977938 1107 5 0.06853045056542584 1107 6 0.06782919849528213 1107 7 0.15430768480194246 1107 8 0.1438660481157399 1107 9 0.20421147104730153 1107 10-11 0.20329663793530983 1107 12-13 0.2113594951924469 1107 14-15 0.36135567769706256 1107 16-17 0.415632950956045 1107 18-19 0.36689933788917983 1107 20-21 0.34267904588148923 1107 22-23 0.3150313846327464 1107 24-25 0.2787536571134126 1107 26-27 0.35651820233623965 1107 28-29 0.3726861238975445 1107 30-31 0.3117649812849095 1107 32-33 0.30672315103841896 1107 34-35 0.40937166124059843 1107 36-37 0.5400350632636588 1107 38-39 0.5693135607582036 1107 40-41 0.6045593875196928 1107 42-43 0.6828705177447816 1107 44-45 0.703975466484799 1107 46-47 0.7728820893907482 1107 48-49 0.6872871658048467 1107 50-51 0.7182658224416159 1107 52-53 0.8937696443430099 1107 54-55 0.8223460878069417 1107 56-57 0.8610711866689158 1107 58-59 0.865080101148969 1107 60-61 0.9887564789712613 1107 62-63 1.1900303648967778 1107 64-65 1.1758883670579365 1107 66-67 1.328565243440618 1107 68-69 1.4683263951953194 1107 70-71 1.3076260407563467 1107 72-73 1.468301686271122 1107 74-75 1.464858391728601 1107 76-77 1.4661751529983142 1107 78-79 1.678965537768402 1107 80-81 1.9569986857971031 1107 82-83 2.0730628716256376 1107 84-85 2.2364828851158016 1107 86-87 2.3329178947564415 1107 88-89 2.6759722078541337 1107 90-91 2.8532990741191533 1107 92-93 3.052835443433569 1107 94-95 3.1219020178007426 1107 96-97 3.334285574854025 1107 98-99 3.74837014819877 1107 100-101 4.039421872796588 1108 1 0.18196931390516724 1108 2 0.1717686923494881 1108 3 0.1768947435982895 1108 4 0.15557392235713507 1108 5 0.06988061122918765 1108 6 0.06687470590926381 1108 7 0.16788793979750238 1108 8 0.1399106949299096 1108 9 0.22692129884630674 1108 10-11 0.20159626776156614 1108 12-13 0.2518015978466863 1108 14-15 0.3474539853061671 1108 16-17 0.34283226782319076 1108 18-19 0.37792919785070467 1108 20-21 0.33116994823955537 1108 22-23 0.2906581989984076 1108 24-25 0.2854083931568425 1108 26-27 0.27341879598484553 1108 28-29 0.28934472964161273 1108 30-31 0.33439728231200405 1108 32-33 0.3733344418754285 1108 34-35 0.3093739593474538 1108 36-37 0.3123246053164266 1108 38-39 0.354284967410635 1108 40-41 0.3267769351952339 1108 42-43 0.4511860058816737 1108 44-45 0.4810689216550301 1108 46-47 0.5229897044241625 1108 48-49 0.5173640924137999 1108 50-51 0.4810310849726278 1108 52-53 0.5679353800551397 1108 54-55 0.6787259231187406 1108 56-57 0.7494537850193055 1108 58-59 0.9123826020980843 1108 60-61 0.8478748839825698 1108 62-63 0.9499097637285061 1108 64-65 1.020434851922687 1108 66-67 1.0841167623038395 1108 68-69 1.2223309036048562 1108 70-71 1.0491706212596021 1108 72-73 1.1815894407904608 1108 74-75 1.2817071955708528 1108 76-77 1.2482682037108965 1108 78-79 1.5843762151848395 1108 80-81 1.9094873180650787 1108 82-83 2.00995939826603 1108 84-85 2.0849373876587585 1108 86-87 2.356579170836575 1108 88-89 2.595281098455871 1108 90-91 2.632388769275277 1108 92-93 2.7820451872051883 1108 94-95 3.007979321995059 1108 96-97 3.3761710900376194 1108 98-99 3.607783301436214 1108 100-101 3.979350870450734 1109 1 0.2912355854403863 1109 2 0.33173195235616504 1109 3 0.2635011051123257 1109 4 0.224187833278215 1109 5 0.16465186790581043 1109 6 0.22700539609831338 1109 7 0.2759993311262292 1109 8 0.28487021354872866 1109 9 0.37907747590320184 1109 10-11 0.3251573051964556 1109 12-13 0.3776815291103972 1109 14-15 0.5285324249742374 1109 16-17 0.4590572084275877 1109 18-19 0.5448962497866177 1109 20-21 0.5500379195555212 1109 22-23 0.40759464689278957 1109 24-25 0.4924430541386471 1109 26-27 0.5493222753714448 1109 28-29 0.5741522483452428 1109 30-31 0.6035661452575809 1109 32-33 0.6046626165797804 1109 34-35 0.6631577205694867 1109 36-37 0.7055696445465784 1109 38-39 0.6842091941321087 1109 40-41 0.653112794272964 1109 42-43 0.8164366996900156 1109 44-45 0.7227638782490615 1109 46-47 0.7398787295413314 1109 48-49 0.7191360602664432 1109 50-51 0.7670395768724703 1109 52-53 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-0.045003505210381434 2316 62-63 0.016944488289077242 2316 64-65 0.009346095515788022 2316 66-67 0.23456606999520702 2316 68-69 0.19708701739893897 2316 70-71 0.3813513475816883 2316 72-73 0.5091761902234104 2316 74-75 0.5937944412345075 2316 76-77 0.6158013640904727 2316 78-79 0.7072431313564529 2316 80-81 0.8060934130915705 2316 82-83 1.032116087256373 2316 84-85 1.157456966479124 2316 86-87 1.1780876373404396 2316 88-89 1.452892280370218 2316 90-91 1.6637316817551842 2316 92-93 1.7008322556228954 2316 94-95 2.3270831298213075 2316 96-97 2.492250043352982 2316 98-99 2.923277405095156 2316 100-101 3.413029145557303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 23.0 4 72.0 5 131.0 6 330.0 7 726.0 8 1752.0 9 3046.0 10 5071.0 11 7840.0 12 11302.0 13 15592.0 14 20719.0 15 27085.0 16 33614.0 17 42194.0 18 41659.0 19 38124.0 20 39447.0 21 42875.0 22 46910.0 23 52780.0 24 61841.0 25 71688.0 26 85664.0 27 102646.0 28 122656.0 29 144949.0 30 172802.0 31 204869.0 32 246023.0 33 299379.0 34 374285.0 35 475504.0 36 608452.0 37 694294.0 38 390841.0 39 23093.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.365286288876966 19.05113798744958 14.696647907328291 23.886927816345167 2 15.836102300201848 20.46066144666504 38.98391855022958 24.719317702903535 3 19.453022387100035 23.714868003643236 29.644793356326755 27.18731625292998 4 13.376640250023945 16.580559822343936 35.95400116356206 34.088798764070056 5 14.000819375250783 37.24484406126051 33.99459638445122 14.759740179037486 6 33.303239172310064 35.68486092240673 16.76997123021856 14.241928675064653 7 29.57276785872654 32.04243276704282 20.13184080484439 18.25295856938626 8 26.753967817576175 35.204182083272734 19.190016247299507 18.85183385185159 9 26.252802481792735 16.86264823887078 19.768737606112957 37.115811673223526 10-11 25.269982759415804 26.313508139613255 27.066032146949375 21.35047695402156 12-13 26.09154892104451 23.390723607893776 27.52653489089832 22.991192580163393 14-15 22.63694690893353 25.28591300599873 25.568112280191418 26.509027804876318 16-17 22.600851652932143 28.483513691365165 26.353882501516534 22.561752154186163 18-19 22.581817391705364 27.147046929153966 28.15968736364507 22.111448315495597 20-21 23.081962836962962 26.21964495393642 28.354807941311066 22.343584267789552 22-23 22.818875424363146 26.102900746027746 28.356371034399576 22.72185279520953 24-25 22.795729583761574 26.40364220126226 28.31139011575771 22.489238099218454 26-27 22.368801659421095 26.644101561378303 28.367616897153518 22.619479882047084 28-29 22.360578747964656 27.089112712979745 28.19421952655795 22.356089012497648 30-31 22.523195858002858 26.518051618876665 28.12044374993349 22.838308773186988 32-33 22.2899885971274 26.58491171365243 28.20241018990672 22.922689499313456 34-35 23.020667737126377 26.59189119008406 27.909283972441834 22.478157100347726 36-37 23.116273696865044 26.184424751055563 28.069143262198114 22.630158289881276 38-39 22.526050934835688 26.596722237707283 28.05908478192563 22.8181420455314 40-41 22.854633806497333 26.47767157946132 28.063218320079887 22.604476293961465 42-43 22.636177294144684 26.432570688823503 27.80187406714877 23.129377949883047 44-45 22.604057526543116 26.568250383051694 27.977922741790955 22.849769348614235 46-47 22.939942167747233 26.942604083200045 27.414758177197633 22.702695571855088 48-49 22.822201154338703 26.739013118452746 27.515870383443364 22.922915343765187 50-51 22.85301958544554 26.931340082939272 27.5346385862721 22.681001745343092 52-53 23.084933822407795 26.821158648849032 27.255321611391558 22.83858591735162 54-55 22.586134762599364 26.74014979534562 27.553678956571943 23.120036485483077 56-57 22.799065159475404 26.895355684603732 27.38200975192715 22.923569403993714 58-59 22.91783806266917 26.575320689055776 27.34692330068501 23.159917947590042 60-61 22.51929366816487 26.881254589507364 27.759446846853237 22.840004895474525 62-63 22.41506529073088 26.9091574640023 27.748959711663645 22.926817533603174 64-65 22.216929384553712 26.910864672056416 27.554515365759347 23.317690577630522 66-67 22.278207937316495 26.845288344231594 27.429420917005658 23.447082801446253 68-69 22.520635641387152 26.58004083561128 27.598004469346577 23.301319053654996 70-71 22.604964472335247 26.586894229370717 27.322800672595633 23.485340625698402 72-73 22.511128449253896 26.601488996154792 27.464527201927503 23.42285535266381 74-75 22.357064559957145 27.18477179284338 26.961149709286858 23.497013937912612 76-77 22.381264788412626 26.64506124664323 27.306316137683446 23.667357827260698 78-79 22.548083403986617 26.711453902721953 27.365259158616922 23.375203534674505 80-81 22.59371993331244 26.957522543190994 27.149705456626755 23.29905206686981 82-83 22.547916455111316 26.805854935628787 27.235443476918995 23.410785132340898 84-85 22.458166915053813 26.766178949829566 27.187633346647903 23.588020788468718 86-87 22.457670053121543 26.819649494049408 27.057863879457845 23.664816573371205 88-89 22.28499111308832 26.864680044019394 26.878204689748507 23.972124153143774 90-91 22.345722329293093 26.69380483686889 27.04304249041489 23.917430343423124 92-93 22.360118378512368 26.485307557538583 27.170431623061813 23.984142440887236 94-95 22.141025140744894 26.324749106930646 27.352998300773695 24.181227451550765 96-97 21.974583441234792 26.264231907142072 27.39067882139677 24.370505830226364 98-99 22.02550036716059 26.392449883466423 27.011479089583634 24.570570659789354 100-101 21.936317956583792 26.03826261553958 27.335374461421324 24.690044966455304 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 727.0 1 510.5 2 381.5 3 506.5 4 463.5 5 449.0 6 873.0 7 1853.5 8 2898.5 9 3304.5 10 2895.5 11 2498.5 12 2589.5 13 2823.5 14 3072.5 15 3304.0 16 3580.0 17 3852.5 18 4146.0 19 4577.5 20 5209.0 21 6014.0 22 7195.5 23 8830.5 24 11088.0 25 14396.5 26 18793.0 27 24333.0 28 29918.0 29 36289.0 30 43896.5 31 52638.0 32 64987.5 33 72268.0 34 75938.0 35 83698.0 36 94584.5 37 105167.0 38 112691.5 39 119408.5 40 126631.0 41 137484.5 42 146916.0 43 154524.0 44 161644.0 45 170224.5 46 176961.5 47 185936.0 48 195243.5 49 191513.0 50 181833.5 51 170295.0 52 162290.0 53 162911.5 54 165969.0 55 156697.5 56 136875.0 57 121086.5 58 109790.5 59 95250.5 60 77403.5 61 64451.5 62 54528.0 63 42153.5 64 31908.0 65 24698.0 66 17939.0 67 14237.0 68 11204.0 69 7635.0 70 5511.5 71 4172.5 72 3244.5 73 2621.0 74 2070.0 75 1586.5 76 1048.5 77 603.5 78 372.5 79 235.5 80 140.0 81 70.0 82 38.5 83 26.5 84 13.5 85 5.5 86 2.0 87 1.0 88 0.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.2171533170387345E-5 2 0.04407700794273005 3 2.6605839804464814E-4 4 0.0 5 0.09790949048043053 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010753193587637862 26-27 0.011584626081527388 28-29 0.0 30-31 0.0 32-33 4.6560219657813427E-4 34-35 0.015497901686100754 36-37 0.003214872309706165 38-39 0.012526916241268851 40-41 0.00730552017964263 42-43 0.005709169791374742 44-45 0.0028046989460539995 46-47 4.434306634077469E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.2171533170387345E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3280109828906713E-4 68-69 1.995437985334861E-4 70-71 0.0 72-73 2.2171533170387345E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.325729975558102E-5 82-83 9.312043931562685E-4 84-85 0.004245848602129176 86-87 0.0014411496560751773 88-89 7.760036609635571E-5 90-91 7.870894275487508E-4 92-93 2.2171533170387345E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 8.203467273043319E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4510288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.700744765078 #Duplication Level Percentage of deduplicated Percentage of total 1 69.78735524107525 30.49759399220057 2 15.299764400217333 13.372221980394485 3 5.7911360181286 7.592308710842642 4 2.8580307107733405 4.995922824890409 5 1.6453884454265575 3.5952350246497224 6 1.020486472471108 2.6757611321804813 7 0.6781021491217393 2.074349826039399 8 0.48022643933885395 1.6789002443991576 9 0.36070424915450583 1.4186739895182143 >10 1.835770024582153 14.567571780537984 >50 0.14269689803241822 4.307992040683068 >100 0.0898982368371936 7.490974484815262 >500 0.006366289889643324 1.890748296547836 >1k 0.0038707037038249945 3.1299469344257638 >5k 1.5279093567729363E-4 0.47547470176732565 >10k+ 5.09303118924312E-5 0.23632403610754404 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10618 0.23541733920317284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8560 0.1897883239385157 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6896 0.15289489274299115 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5907 0.13096724643747806 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2171533170387345E-5 2 0.0 0.0 0.0 0.0 2.2171533170387345E-5 3 0.0 0.0 0.0 2.2171533170387345E-5 2.2171533170387345E-5 4 0.0 0.0 0.0 2.2171533170387345E-5 2.2171533170387345E-5 5 0.0 0.0 0.0 2.2171533170387345E-5 2.2171533170387345E-5 6 0.0 0.0 0.0 2.2171533170387345E-5 2.2171533170387345E-5 7 0.0 0.0 0.0 2.2171533170387345E-5 2.2171533170387345E-5 8 0.0 0.0 0.0 4.434306634077469E-5 2.2171533170387345E-5 9 0.0 0.0 0.0 6.651459951116203E-5 2.2171533170387345E-5 10-11 0.0 0.0 0.0 9.977189926674305E-5 2.2171533170387345E-5 12-13 0.0 0.0 0.0 1.219434324371304E-4 1.5520073219271141E-4 14-15 0.0 0.0 0.0 1.3302919902232407E-4 1.5520073219271141E-4 16-17 0.0 0.0 0.0 1.3302919902232407E-4 1.5520073219271141E-4 18-19 0.0 0.0 0.0 1.7737226536309876E-4 1.5520073219271141E-4 20-21 0.0 0.0 0.0 2.771441646298418E-4 1.5520073219271141E-4 22-23 0.0 0.0 0.0 4.545164299929406E-4 1.5520073219271141E-4 24-25 0.0 0.0 0.0 6.208029287708458E-4 1.5520073219271141E-4 26-27 0.0 0.0 0.0 0.0010420620590082053 1.5520073219271141E-4 28-29 0.0 0.0 0.0 0.0035031022409212006 1.5520073219271141E-4 30-31 0.0 0.0 0.0 0.012194343243713041 1.5520073219271141E-4 32-33 0.0 0.0 0.0 0.03117317563756461 1.8845803194829244E-4 34-35 0.0 0.0 0.0 0.059597081162001186 1.995437985334861E-4 36-37 0.0 0.0 0.0 0.10808622420563832 1.995437985334861E-4 38-39 0.0 0.0 0.0 0.18351378005129607 1.995437985334861E-4 40-41 0.0 0.0 0.0 0.2678875495312051 1.995437985334861E-4 42-43 0.0 0.0 0.0 0.35557596322008705 2.1062956511867976E-4 44-45 0.0 0.0 0.0 0.4663782002390978 2.3280109828906713E-4 46-47 0.0 0.0 0.0 0.5986868244333843 2.5497263145945445E-4 48-49 0.0 0.0 0.0 0.7365383319202676 2.6605839804464814E-4 50-51 0.0 0.0 0.0 0.8858857793559969 2.882299312150355E-4 52-53 0.0 0.0 0.0 1.0333929895385838 3.214872309706165E-4 54-55 0.0 0.0 0.0 1.1949680375177816 3.325729975558102E-4 56-57 0.0 0.0 0.0 1.3884811790289224 3.325729975558102E-4 58-59 0.0 0.0 0.0 1.5966718754988594 3.325729975558102E-4 60-61 0.0 0.0 0.0 1.8224335120063286 3.547445307261975E-4 62-63 0.0 1.1085766585193672E-5 0.0 2.047962347415509 3.547445307261975E-4 64-65 0.0 2.2171533170387345E-5 0.0 2.2844882632772006 3.547445307261975E-4 66-67 0.0 2.2171533170387345E-5 0.0 2.5589718439265963 3.547445307261975E-4 68-69 0.0 2.2171533170387345E-5 0.0 2.860327322778501 3.547445307261975E-4 70-71 0.0 2.2171533170387345E-5 0.0 3.182701415075933 3.769160638965849E-4 72-73 0.0 2.2171533170387345E-5 0.0 3.512148226454719 4.101733636521659E-4 74-75 0.0 2.2171533170387345E-5 0.0 3.8474815798902418 4.545164299929406E-4 76-77 0.0 2.2171533170387345E-5 0.0 4.213356220267974 4.6560219657813427E-4 78-79 0.0 2.2171533170387345E-5 0.0 4.6233965547211175 4.6560219657813427E-4 80-81 0.0 2.2171533170387345E-5 0.0 5.0601979297109185 4.6560219657813427E-4 82-83 0.0 2.2171533170387345E-5 0.0 5.50214310039625 4.6560219657813427E-4 84-85 0.0 2.2171533170387345E-5 0.0 5.961016236657171 4.6560219657813427E-4 86-87 0.0 2.2171533170387345E-5 0.0 6.429988506277205 4.766879631633279E-4 88-89 0.0 2.2171533170387345E-5 0.0 6.935421862195939 4.877737297485216E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19420 0.0 26.646109 1 GTACATG 16200 0.0 25.806433 1 TACATGG 15955 0.0 25.576706 2 ACATGGG 16100 0.0 24.331963 3 GGTATCA 14235 0.0 24.08973 1 TAAGGTG 2100 0.0 22.638962 5 ATCAACG 22775 0.0 22.440157 3 TCAACGC 23045 0.0 22.239132 4 CAACGCA 23180 0.0 22.048082 5 TATCAAC 23350 0.0 21.957659 2 AACGCAG 23920 0.0 21.384624 6 GTAAGGT 1980 0.0 21.129698 4 CATGGGG 8610 0.0 20.706366 4 AGTACTT 12375 0.0 20.187737 12-13 AGAGTAC 19780 0.0 20.097696 10-11 GAGTACT 11770 0.0 20.095552 12-13 GTACTTT 12270 0.0 19.083122 14-15 ACGCAGA 27200 0.0 18.718584 7 CGCAGAG 27065 0.0 18.689112 8 TACTTTT 14175 0.0 17.43993 14-15 >>END_MODULE