FastQCFastQC Report
Thu 26 May 2016
SRR938548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508280
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99250.22015047867479393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76170.1689557880167159No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68250.1513881125395938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53480.11862617228743558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194400.026.1034761
GTACATG155500.024.3524761
GGTATCA142550.023.9216861
TACATGG154650.023.7264522
ACATGGG159200.022.5407963
ATCAACG226900.022.1414413
TATCAAC229550.021.989362
TCAACGC230650.021.7814584
CAACGCA231700.021.6002145
AACGCAG239750.020.9295466
GAGTACT110950.019.75895512-13
AGAGTAC191500.019.6707810-11
GTGTAAG18650.019.1794171
AGTACTT118100.019.08560412-13
ACGCAGA269600.018.5554497
GTACTTT115900.018.52652214-15
CGCAGAG269050.018.3991458
CATGGGA77000.017.900814
CATGGGG88200.017.6215694
TAAGGTG21450.017.5047365
Produced by FastQC (version 0.11.5)