##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938548_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4508280 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.117873778913467 31.0 31.0 34.0 28.0 34.0 2 31.292681022474202 31.0 31.0 34.0 28.0 34.0 3 31.357204965086463 31.0 31.0 34.0 28.0 34.0 4 35.01816080633856 37.0 35.0 37.0 32.0 37.0 5 35.0127339029519 37.0 35.0 37.0 32.0 37.0 6 34.98672731063731 37.0 35.0 37.0 32.0 37.0 7 34.902628053270874 37.0 35.0 37.0 32.0 37.0 8 34.861013069285846 37.0 35.0 37.0 32.0 37.0 9 36.3464239133328 39.0 35.0 39.0 32.0 39.0 10-11 36.18541694393427 39.0 35.0 39.0 32.0 39.0 12-13 36.012063248068 38.0 35.0 39.0 30.0 39.0 14-15 37.18692006707658 39.0 36.0 41.0 31.0 41.0 16-17 37.119469065807806 39.0 36.0 41.0 31.0 41.0 18-19 37.103230500323846 39.0 36.0 41.0 31.0 41.0 20-21 37.03801904495728 39.0 36.0 41.0 31.0 41.0 22-23 36.89159590797377 39.0 36.0 41.0 30.0 41.0 24-25 36.75861503722041 39.0 36.0 41.0 30.0 41.0 26-27 36.5549584320406 39.0 36.0 41.0 30.0 41.0 28-29 36.33576452660438 39.0 35.5 40.5 29.5 41.0 30-31 36.08925820933926 39.0 35.0 40.0 29.0 41.0 32-33 35.81271183245052 38.0 35.0 40.0 27.5 41.0 34-35 35.527514040831534 38.0 34.0 40.0 27.0 41.0 36-37 35.34042827863398 38.0 34.0 40.0 26.5 41.0 38-39 35.123193878818526 38.0 34.0 40.0 25.5 41.0 40-41 34.87181042437471 38.0 34.0 40.0 25.0 41.0 42-43 34.49200393498185 38.0 33.0 40.0 24.0 41.0 44-45 34.064500873947495 38.0 33.0 40.0 23.5 41.0 46-47 33.61427362541812 38.0 32.5 40.0 21.0 41.0 48-49 33.165961630599696 37.0 31.5 40.0 18.5 41.0 50-51 32.44167443015962 36.5 30.5 39.5 17.5 40.5 52-53 32.45091897575128 37.0 31.0 39.0 16.0 40.0 54-55 32.46244432466484 37.0 31.0 39.0 14.0 40.5 56-57 32.055633301392106 36.0 31.0 39.0 9.0 41.0 58-59 31.618609092602945 36.0 30.0 39.0 7.5 40.0 60-61 31.07486768789871 35.5 29.0 39.0 4.5 40.0 62-63 30.380570971634413 35.0 28.0 38.5 2.0 40.0 64-65 29.743355226383454 34.0 28.0 38.0 2.0 40.0 66-67 29.066729772773655 34.0 26.5 37.5 2.0 40.0 68-69 28.245226782719797 34.0 26.0 37.0 2.0 39.0 70-71 27.422020038684376 33.0 24.0 36.0 2.0 39.0 72-73 26.54577710346296 32.0 21.5 35.5 2.0 38.5 74-75 25.635077013850072 31.5 19.0 35.0 2.0 38.0 76-77 24.70614547011277 31.0 14.0 35.0 2.0 37.0 78-79 23.732175574720294 30.0 7.5 34.0 2.0 36.0 80-81 22.773285377128303 29.5 2.0 34.0 2.0 36.0 82-83 21.781902077954342 29.0 2.0 34.0 2.0 35.0 84-85 20.801378463626925 27.5 2.0 33.5 2.0 35.0 86-87 19.82537719485036 26.0 2.0 33.0 2.0 35.0 88-89 18.74259495860949 25.0 2.0 32.0 2.0 34.5 90-91 17.58961344459528 21.5 2.0 32.0 2.0 34.0 92-93 16.420618284578598 15.5 2.0 31.0 2.0 34.0 94-95 15.147872248396283 4.0 2.0 31.0 2.0 34.0 96-97 13.698466155607015 2.0 2.0 30.5 2.0 34.0 98-99 11.944695205266754 2.0 2.0 29.0 2.0 34.0 100-101 8.427652896448313 2.0 2.0 12.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4854177335268517 1101 2 0.4323391244961492 1101 3 0.4073733687414496 1101 4 0.3518065845744971 1101 5 0.3315697747537101 1101 6 0.41022150328799967 1101 7 0.41124490340892805 1101 8 0.392219187997334 1101 9 0.4363433339944365 1101 10-11 0.5493708915058946 1101 12-13 0.39305579669478874 1101 14-15 0.4556221189618057 1101 16-17 0.506745627328776 1101 18-19 0.4192936425596798 1101 20-21 0.5552284720392606 1101 22-23 0.6198686462250436 1101 24-25 0.6685353182273985 1101 26-27 0.6526773567640518 1101 28-29 0.7427635491351552 1101 30-31 0.7993618945803718 1101 32-33 0.9207825307825388 1101 34-35 0.8884369165877288 1101 36-37 0.9984395060859654 1101 38-39 1.0592604596922683 1101 40-41 1.0386101212817849 1101 42-43 1.1545398894401941 1101 44-45 1.1149375771150432 1101 46-47 1.0657997884762338 1101 48-49 1.0519937147446967 1101 50-51 1.1546646558605502 1101 52-53 1.153272157862041 1101 54-55 1.3979775138917248 1101 56-57 1.6786664425788302 1101 58-59 1.602852564450373 1101 60-61 1.683031932838194 1101 62-63 1.584888917710657 1101 64-65 1.5569277826072074 1101 66-67 1.8464265388526506 1101 68-69 1.9809617728499767 1101 70-71 2.2799516669029387 1101 72-73 2.2913150235729525 1101 74-75 2.1981035798117077 1101 76-77 2.575094322296451 1101 78-79 2.7918460256031317 1101 80-81 3.1710730297703016 1101 82-83 3.1778442564220732 1101 84-85 3.104649627456201 1101 86-87 3.1594540778690323 1101 88-89 3.671652449192422 1101 90-91 3.8207471689044787 1101 92-93 3.819152714571061 1101 94-95 3.6325282765929874 1101 96-97 3.5758719713599945 1101 98-99 3.383566051934407 1101 100-101 2.726401170049547 1102 1 0.5974147675223556 1102 2 0.4400407465872682 1102 3 0.4242107516252247 1102 4 0.4044276481565632 1102 5 0.4374127449224545 1102 6 0.4328508111581968 1102 7 0.4879821045407269 1102 8 0.4908680535337808 1102 9 0.5138413009902365 1102 10-11 0.663835630269638 1102 12-13 0.6993478139223086 1102 14-15 0.7425683820742606 1102 16-17 0.7722623867596283 1102 18-19 0.7370898034924735 1102 20-21 0.6435268456103884 1102 22-23 0.5883090787554579 1102 24-25 0.7251589824217959 1102 26-27 0.6464395678663095 1102 28-29 0.690659380219877 1102 30-31 0.5828043990272107 1102 32-33 0.7247193059466852 1102 34-35 0.7200618119393667 1102 36-37 0.8105521540385325 1102 38-39 0.8870218177875557 1102 40-41 0.9817418522990593 1102 42-43 1.14778488692302 1102 44-45 1.0909239190521873 1102 46-47 1.193743912819805 1102 48-49 1.1049382920010302 1102 50-51 1.177533624752968 1102 52-53 1.2267021070567097 1102 54-55 1.2410343420683532 1102 56-57 1.496582306441681 1102 58-59 1.6599099723553508 1102 60-61 1.4884468302745013 1102 62-63 1.5256255694784038 1102 64-65 1.600048841468773 1102 66-67 1.784699345179245 1102 68-69 1.9153918057378192 1102 70-71 1.8042239636707755 1102 72-73 1.9188197268487492 1102 74-75 1.9223113779743883 1102 76-77 2.037285643109996 1102 78-79 2.5656359064857988 1102 80-81 3.0043022888534985 1102 82-83 3.013847127761423 1102 84-85 3.163803128395326 1102 86-87 3.3683315444255726 1102 88-89 3.2171143653527814 1102 90-91 3.0483487508585974 1102 92-93 3.1576110747921042 1102 94-95 3.107021346007036 1102 96-97 3.0669153662716013 1102 98-99 2.917538815164116 1102 100-101 2.205439410431147 1103 1 0.3772688922040679 1103 2 0.3772663131231795 1103 3 0.3678174883354117 1103 4 0.29391467557461937 1103 5 0.3103643678740724 1103 6 0.3434711010888307 1103 7 0.3589799410558001 1103 8 0.31318530688552215 1103 9 0.3239853007894098 1103 10-11 0.4437353455141775 1103 12-13 0.4210764149612558 1103 14-15 0.5096610112358562 1103 16-17 0.46003621677484574 1103 18-19 0.5315587057199949 1103 20-21 0.5355665523052622 1103 22-23 0.6343415773741725 1103 24-25 0.6734937411013817 1103 26-27 0.6065391357245034 1103 28-29 0.6441177216092555 1103 30-31 0.5850859185501136 1103 32-33 0.7676185772942361 1103 34-35 0.7085173521190455 1103 36-37 0.7700511997444792 1103 38-39 0.7482384721076585 1103 40-41 0.8477706795347828 1103 42-43 0.9663796753453937 1103 44-45 1.067224520352795 1103 46-47 1.131586898076577 1103 48-49 1.0844363878784335 1103 50-51 1.100303211315662 1103 52-53 1.2238346084995513 1103 54-55 1.3435134488578058 1103 56-57 1.3599784370791852 1103 58-59 1.42774764967578 1103 60-61 1.5320566325049931 1103 62-63 1.7294583207249445 1103 64-65 1.7863256053317436 1103 66-67 1.9867317670392524 1103 68-69 2.0688915195823654 1103 70-71 2.0502220717378457 1103 72-73 2.3706923252730725 1103 74-75 2.2993693800323918 1103 76-77 2.410317011831019 1103 78-79 2.5629874598718345 1103 80-81 2.693232447611244 1103 82-83 2.7323448333280957 1103 84-85 2.8322511459812745 1103 86-87 2.879733982552146 1103 88-89 3.321459185214845 1103 90-91 3.1786427475319066 1103 92-93 3.189894171730373 1103 94-95 3.0903328767323597 1103 96-97 2.96241615401377 1103 98-99 2.726673154470868 1103 100-101 2.2887916920592897 1104 1 0.5317331505705312 1104 2 0.5872307772645975 1104 3 0.5456594520577589 1104 4 0.4001707353546138 1104 5 0.37908802297186384 1104 6 0.46415763761513773 1104 7 0.43184421145132745 1104 8 0.4420143499456728 1104 9 0.5282270493251744 1104 10-11 0.6062542035773575 1104 12-13 0.5651911399273004 1104 14-15 0.7194468771975622 1104 16-17 0.7659804119441631 1104 18-19 0.7299354782665475 1104 20-21 0.6978397612974376 1104 22-23 0.7086041428726944 1104 24-25 0.817959982035724 1104 26-27 0.7577567164236427 1104 28-29 0.8370127774970584 1104 30-31 0.7026614385147383 1104 32-33 0.786955965238775 1104 34-35 0.8888622521023919 1104 36-37 0.9134408394907254 1104 38-39 0.9135737739007084 1104 40-41 0.9319143689265417 1104 42-43 1.035979837069469 1104 44-45 1.07461159055741 1104 46-47 1.0960846484746227 1104 48-49 1.0833078278185937 1104 50-51 1.0890676761253602 1104 52-53 1.068080957038326 1104 54-55 1.3811374359243374 1104 56-57 1.4935054420484164 1104 58-59 1.628886512405792 1104 60-61 1.5932766023098175 1104 62-63 1.7732806726184975 1104 64-65 1.9167268358603913 1104 66-67 2.1152427638866342 1104 68-69 2.25485794624322 1104 70-71 2.3033177456744873 1104 72-73 2.4664916481927293 1104 74-75 2.4761866735933324 1104 76-77 2.729898405988351 1104 78-79 2.812067152974784 1104 80-81 2.946273879381671 1104 82-83 3.234872830138162 1104 84-85 3.3513771342718712 1104 86-87 3.4183881252558237 1104 88-89 3.491037711331554 1104 90-91 3.467791808234683 1104 92-93 3.424006396210519 1104 94-95 3.423297495135378 1104 96-97 3.447202061743056 1104 98-99 3.3330609770488646 1104 100-101 2.2351720298866287 1105 1 0.5192115643191535 1105 2 0.41003574158226286 1105 3 0.3288403812548566 1105 4 0.30532854905746376 1105 5 0.37762796013766575 1105 6 0.4490670273744115 1105 7 0.5003194418780623 1105 8 0.5074846701504043 1105 9 0.5077351948841269 1105 10-11 0.5900868815208895 1105 12-13 0.5608593254338246 1105 14-15 0.6598982119040997 1105 16-17 0.6733384628357086 1105 18-19 0.6572849986876648 1105 20-21 0.6603436624272021 1105 22-23 0.6793376072839905 1105 24-25 0.7334422657050794 1105 26-27 0.5790847130114543 1105 28-29 0.6516578787183747 1105 30-31 0.7452042989271135 1105 32-33 0.8618189055462864 1105 34-35 0.8395938439714001 1105 36-37 0.8798214233078028 1105 38-39 0.926586382352113 1105 40-41 0.9594820925392966 1105 42-43 1.0990361381742701 1105 44-45 1.201109104237375 1105 46-47 1.2602103792862707 1105 48-49 1.2279112649740043 1105 50-51 1.1049985897179369 1105 52-53 1.1179558608104614 1105 54-55 1.4132190267530333 1105 56-57 1.5331063304656993 1105 58-59 1.5231482105935896 1105 60-61 1.7492201035477741 1105 62-63 1.6820194633722991 1105 64-65 1.9676789715989038 1105 66-67 2.091756402236303 1105 68-69 2.252428842774858 1105 70-71 2.412845084791897 1105 72-73 2.381932839961866 1105 74-75 2.462138705301715 1105 76-77 2.5441299874543404 1105 78-79 2.796604374954267 1105 80-81 3.0166271136783216 1105 82-83 3.2014222028365005 1105 84-85 3.1567710963632933 1105 86-87 3.4714346475286746 1105 88-89 3.5661258768642945 1105 90-91 3.4109989010087496 1105 92-93 3.481672636353668 1105 94-95 3.4252020266877192 1105 96-97 3.4435545054107415 1105 98-99 3.2948286049539046 1105 100-101 2.424708679700416 1106 1 0.20145828295544632 1106 2 0.10887343024429796 1106 3 0.011934075754904683 1106 4 0.026369796167180937 1106 5 0.07628875451898409 1106 6 -0.2780021317377077 1106 7 0.016484202521453994 1106 8 -0.2432891289339807 1106 9 -0.06515311074080898 1106 10-11 0.047108727712576126 1106 12-13 0.20111350890796587 1106 14-15 0.3432420026628549 1106 16-17 -0.20650052809077835 1106 18-19 0.24859641527319098 1106 20-21 0.30001923529253105 1106 22-23 0.18558875382099416 1106 24-25 0.229552053048927 1106 26-27 0.16803212148504088 1106 28-29 0.2651009621015987 1106 30-31 0.14639148129856494 1106 32-33 0.29421386763748814 1106 34-35 0.12622877550172262 1106 36-37 0.2164493244244028 1106 38-39 0.3949197358443399 1106 40-41 0.4582924407035378 1106 42-43 0.15057904940055522 1106 44-45 0.5495667473806307 1106 46-47 0.8054569628579813 1106 48-49 0.6730156275598986 1106 50-51 0.5339575693957528 1106 52-53 0.6064814444607975 1106 54-55 0.5087386373235532 1106 56-57 1.0488433950084186 1106 58-59 0.9443964895281454 1106 60-61 1.2899254178349828 1106 62-63 1.2667269024067664 1106 64-65 1.0863135241420458 1106 66-67 1.3758932186230588 1106 68-69 1.4965712734131493 1106 70-71 1.3150356256348203 1106 72-73 1.4262715174175717 1106 74-75 1.7697222538312225 1106 76-77 1.9041048845935933 1106 78-79 2.2939860120176014 1106 80-81 2.1672887537251704 1106 82-83 2.2973906007488445 1106 84-85 2.2481405705589026 1106 86-87 1.975422832401847 1106 88-89 1.9196762357933714 1106 90-91 1.8002521702771226 1106 92-93 2.0150666227544782 1106 94-95 1.973895352481625 1106 96-97 1.9039228537322117 1106 98-99 1.798532221862379 1106 100-101 1.3100483267988459 1107 1 0.31794544374652745 1107 2 0.2914238077306024 1107 3 0.21496319070814351 1107 4 0.2648034349022126 1107 5 0.2720031144015209 1107 6 0.2666662190318263 1107 7 0.32650148865086237 1107 8 0.35879784119816094 1107 9 0.46259334927139406 1107 10-11 0.3771042284911488 1107 12-13 0.38167858219058104 1107 14-15 0.3635384531727581 1107 16-17 0.34735310247534557 1107 18-19 0.48435386978479045 1107 20-21 0.5687728321791425 1107 22-23 0.5074606789858294 1107 24-25 0.5508474705674828 1107 26-27 0.5574767823067788 1107 28-29 0.5406384260465202 1107 30-31 0.6124440558072806 1107 32-33 0.6831983329188702 1107 34-35 0.6317111539183315 1107 36-37 0.7703652419552114 1107 38-39 0.6813710685794376 1107 40-41 0.6964186438166067 1107 42-43 0.9660657105548651 1107 44-45 0.9361385686332824 1107 46-47 0.8813909991184303 1107 48-49 0.7233888272965956 1107 50-51 0.9348661927661936 1107 52-53 1.1795497535045243 1107 54-55 1.2539761151770747 1107 56-57 1.4617517781394014 1107 58-59 1.382730502645913 1107 60-61 1.34495991442029 1107 62-63 1.521908239134362 1107 64-65 1.4831803676867708 1107 66-67 1.7573085125658103 1107 68-69 1.7704254566832844 1107 70-71 1.9260334872354328 1107 72-73 2.0459303563138356 1107 74-75 2.2140066477304465 1107 76-77 2.3235226506654953 1107 78-79 2.787055635289949 1107 80-81 2.820341787256009 1107 82-83 2.874059651929425 1107 84-85 2.7806241446170255 1107 86-87 2.777959815676276 1107 88-89 3.052562613258221 1107 90-91 2.9236450660093674 1107 92-93 2.8186593233831623 1107 94-95 2.8171934187647256 1107 96-97 2.8891001366584277 1107 98-99 2.531646180234665 1107 100-101 1.8921023147738456 1108 1 0.2956979087077052 1108 2 0.2548399306293554 1108 3 0.11012253091239899 1108 4 0.14507618312686077 1108 5 0.1435749007691527 1108 6 0.20276583659372704 1108 7 0.14213531137531987 1108 8 0.26652920364288235 1108 9 0.2984533578420425 1108 10-11 0.233497800326127 1108 12-13 0.27686949964589047 1108 14-15 0.27419148782986014 1108 16-17 0.4108224768181401 1108 18-19 0.3420108917731781 1108 20-21 0.3927682794732661 1108 22-23 0.41480631731894846 1108 24-25 0.4580088670067042 1108 26-27 0.42895149619842243 1108 28-29 0.41238378601368453 1108 30-31 0.4081597228052516 1108 32-33 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0.3833291394104137 2316 92-93 0.3451200123010416 2316 94-95 0.3513600724886192 2316 96-97 0.4156007863855926 2316 98-99 0.3411792923760206 2316 100-101 0.5506672913733137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12394.0 3 4723.0 4 5316.0 5 6068.0 6 7472.0 7 9571.0 8 12538.0 9 16221.0 10 20725.0 11 26379.0 12 32336.0 13 38529.0 14 44163.0 15 50929.0 16 58440.0 17 67181.0 18 68121.0 19 69882.0 20 76611.0 21 85931.0 22 95172.0 23 106401.0 24 118610.0 25 131872.0 26 146894.0 27 162622.0 28 180874.0 29 201393.0 30 224047.0 31 252115.0 32 285349.0 33 323683.0 34 368736.0 35 408562.0 36 410993.0 37 299130.0 38 77099.0 39 1198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7569264745472 18.215484084445038 14.037298807972798 23.99029063303496 2 15.50514770389021 19.823751990766482 39.88238003593475 24.78872026940856 3 19.473362358985874 23.529372608345845 29.108808032629675 27.888457000038606 4 12.964167415041933 15.898042552096129 36.27224571828381 34.86554431457813 5 14.149862335993395 37.09552805604662 33.779384690164434 14.975224917795558 6 33.3857302422486 35.2525559860195 17.009332686194888 14.352381085537012 7 29.7483771817126 31.941861234003145 20.07464054358588 18.23512104069838 8 26.783887595720934 34.79249277169656 19.376158026356958 19.04746160622555 9 26.578844248155985 16.909295531631912 19.783542271962965 36.728317948249135 10-11 25.34752886606448 26.104960729371268 27.066922494539213 21.48058791002504 12-13 26.19355206592885 23.311150631994543 27.449154273186583 23.04614302889002 14-15 22.8303823351458 25.112205823720206 25.56237143949383 26.495040401640164 16-17 22.773443423503753 28.32580357064612 26.22137846184292 22.679374544007207 18-19 22.741151743758945 27.02964480065035 28.060666530438098 22.16853692515261 20-21 23.245182040167876 26.210211822709283 28.18344802949298 22.361158107629855 22-23 22.925880583751628 26.043334832891695 28.185924527652983 22.84486005570369 24-25 22.7949328887915 26.29871768912213 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22.99623306980684 50-51 22.907265585095143 26.933682062783504 27.391156536153783 22.76789581596757 52-53 23.11462372556501 26.78263396541168 27.183354481485754 22.91938782753756 54-55 22.596285664849134 26.67727387675967 27.506965487561946 23.21947497082925 56-57 22.85210094006718 26.87694830601014 27.33346680205793 22.937483951864746 58-59 22.978382741684932 26.502486296366705 27.332390440253135 23.186740521695228 60-61 22.566415556413293 26.736010363843672 27.755225178461306 22.94234890128173 62-63 22.47987613386177 26.761062206042258 27.821187396560536 22.937874263535434 64-65 22.300606825043147 26.765530793617483 27.629178667167704 23.304683714171663 66-67 22.3776316072588 26.71222362980296 27.435883045937683 23.474261717000555 68-69 22.577773577857428 26.45834470195576 27.653957766843195 23.309923953343617 70-71 22.724583410515482 26.414818084914042 27.422042333899398 23.43855617067108 72-73 22.61530492498608 26.434271035065652 27.54644696006726 23.40397707988101 74-75 22.492900351358152 26.958333384171212 27.137678334200537 23.411087930270096 76-77 22.65875948455359 26.4925538261691 27.235801438470062 23.61288525080725 78-79 22.857502860537537 26.512202815771623 27.274421270775225 23.35587305291562 80-81 22.94603866883034 26.693993586622195 27.036691131858028 23.323276612689437 82-83 22.888389917243003 26.614323818794688 27.104904912477284 23.392381351485025 84-85 22.85507031779016 26.515344357246207 27.10203302959065 23.527552295372985 86-87 22.885306839918773 26.524262123408963 26.955909905739322 23.63452113093294 88-89 22.835171727060732 26.50510409799797 26.824342469340973 23.835381705600327 90-91 22.888295590527544 26.423499141206353 26.839744768059155 23.848460500206947 92-93 23.02247358634027 26.062904745557606 27.033223728176253 23.881397939925876 94-95 22.818308327246125 25.925962902137094 27.16653327498487 24.089195495631905 96-97 22.79901448401454 25.802018486355976 27.112948646657063 24.286018382972415 98-99 22.898332970444507 25.849445467792165 26.64573040314379 24.606491158619537 100-101 23.787180690697582 24.800755614614783 26.88037253061939 24.531691164068242 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 584.0 1 431.5 2 369.5 3 467.5 4 448.0 5 467.5 6 751.0 7 1595.0 8 2449.5 9 2788.5 10 2608.5 11 2306.5 12 2339.5 13 2509.5 14 2842.0 15 3124.0 16 3332.5 17 3743.5 18 4104.5 19 4497.0 20 5168.0 21 5980.5 22 7193.0 23 8942.0 24 11142.0 25 14312.5 26 18678.0 27 23872.5 28 29389.0 29 35678.5 30 42926.5 31 51937.0 32 63434.5 33 70700.5 34 75004.5 35 82537.5 36 93283.5 37 103285.5 38 111181.5 39 118717.0 40 125607.5 41 135447.0 42 145590.5 43 153164.5 44 159154.0 45 167126.0 46 174789.0 47 182961.0 48 190094.0 49 188649.0 50 180684.0 51 170259.0 52 163479.0 53 162100.0 54 162053.0 55 153351.0 56 137250.0 57 123584.0 58 112241.0 59 98322.0 60 81735.5 61 68398.5 62 57914.0 63 46213.5 64 35919.0 65 28335.5 66 21561.0 67 16948.5 68 13179.0 69 9370.0 70 7067.5 71 5429.0 72 4183.5 73 3289.5 74 2433.0 75 1884.0 76 1385.0 77 844.0 78 525.5 79 347.5 80 208.0 81 128.0 82 84.5 83 51.0 84 28.5 85 16.5 86 18.5 87 14.5 88 8.5 89 6.0 90 5.0 91 2.5 92 1.0 93 1.0 94 2.0 95 2.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38504706894869 2 0.02710568110232727 3 0.0324292191256976 4 0.041057787005243686 5 0.06088796614229817 6 0.05077324389789454 7 0.035800793207165484 8 0.03748658024789942 9 0.0340262805327087 10-11 0.03557897912285839 12-13 0.027493855749864692 14-15 0.02575261518805398 16-17 0.020950340262805327 18-19 0.025486438286885467 20-21 0.03205213518237554 22-23 0.030998518281916826 24-25 0.03920563940127943 26-27 0.03389319208212444 28-29 0.029567817438136055 30-31 0.0320632258865909 32-33 0.027083499693896566 34-35 0.028525291241892698 36-37 0.03049943659222586 38-39 0.014218282804084928 40-41 0.012000141961013957 42-43 0.01246595153805886 44-45 0.010968706468985954 46-47 0.01958618364431668 48-49 0.01693550533684687 50-51 0.010813436609970986 52-53 0.008872563372283888 54-55 0.007541678866441303 56-57 0.007929853513978723 58-59 0.003748658024789942 60-61 0.006654422529212916 62-63 0.01132360900387731 64-65 0.006842964500873947 66-67 0.0033493926730371673 68-69 0.0057228033751231065 70-71 0.012088867594736796 72-73 0.009981633793819373 74-75 0.010791255201540277 76-77 0.005024089009555751 78-79 0.00856202365425395 80-81 0.011423425341815504 82-83 0.012521405059135633 84-85 0.007818946471825175 86-87 0.0020074174629792292 88-89 0.015005722803375124 90-91 0.024077918851535398 92-93 0.009593459146281952 94-95 0.019464185897947774 96-97 0.01760094758976816 98-99 0.010458534075079632 100-101 0.12101067369373686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4508280.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.76319143470158 #Duplication Level Percentage of deduplicated Percentage of total 1 72.84152631138276 34.06302239295045 2 13.764669021229606 12.873597049501328 3 5.275320519255505 7.4007246996406355 4 2.57851612030554 4.823185718052478 5 1.4389904870730406 3.364589380985554 6 0.9135321320813229 2.5631806784561997 7 0.6209519873293818 2.0326387658669587 8 0.4246822327058147 1.58875972375508 9 0.303129665877016 1.2757779535449625 >10 1.6254418941172535 13.72375841796668 >50 0.12486760584587582 4.026682478820777 >100 0.07987127690430211 7.124056424901978 >500 0.005062242208402863 1.6403143032909442 >1k 0.003247475701701672 2.8360959719584957 >5k 1.9102798245303123E-4 0.6636160403072979 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9925 0.22015047867479393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7617 0.1689557880167159 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6825 0.1513881125395938 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5348 0.11862617228743558 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.2181408430709717E-5 0.0 4 0.0 0.0 0.0 2.2181408430709717E-5 0.0 5 0.0 0.0 0.0 2.2181408430709717E-5 0.0 6 0.0 0.0 0.0 2.2181408430709717E-5 0.0 7 0.0 0.0 0.0 2.2181408430709717E-5 0.0 8 0.0 0.0 0.0 2.2181408430709717E-5 0.0 9 0.0 0.0 0.0 2.2181408430709717E-5 0.0 10-11 0.0 0.0 0.0 5.54535210767743E-5 0.0 12-13 0.0 0.0 0.0 7.763492950748401E-5 0.0 14-15 0.0 0.0 0.0 8.872563372283887E-5 0.0 16-17 0.0 0.0 0.0 1.330884505842583E-4 0.0 18-19 0.0 0.0 0.0 1.9963267587638745E-4 0.0 20-21 0.0 0.0 0.0 2.5508619695316175E-4 0.0 22-23 0.0 0.0 0.0 3.6599323910671035E-4 0.0 24-25 0.0 0.0 0.0 5.656259149830978E-4 0.0 26-27 0.0 0.0 0.0 0.0011867053510429698 2.2181408430709717E-5 28-29 0.0 0.0 0.0 0.0036155695742056838 2.2181408430709717E-5 30-31 0.0 0.0 0.0 0.012432679425412796 2.2181408430709717E-5 32-33 0.0 0.0 0.0 0.03102069969034754 2.2181408430709717E-5 34-35 0.0 0.0 0.0 0.05953490022802488 2.2181408430709717E-5 36-37 0.0 0.0 0.0 0.10925452722546071 2.2181408430709717E-5 38-39 0.0 0.0 0.0 0.18611310743786988 2.2181408430709717E-5 40-41 0.0 0.0 0.0 0.2707684527136735 2.2181408430709717E-5 42-43 1.1090704215354859E-5 0.0 0.0 0.3593166351690667 2.2181408430709717E-5 44-45 2.2181408430709717E-5 0.0 0.0 0.4692255139432333 2.2181408430709717E-5 46-47 2.2181408430709717E-5 0.0 0.0 0.598543125094271 2.2181408430709717E-5 48-49 2.2181408430709717E-5 0.0 0.0 0.7366445739838696 2.2181408430709717E-5 50-51 2.2181408430709717E-5 0.0 0.0 0.8889975777901994 2.2181408430709717E-5 52-53 2.2181408430709717E-5 0.0 0.0 1.038067733148784 3.327211264606458E-5 54-55 2.2181408430709717E-5 0.0 0.0 1.1996149307496429 6.654422529212916E-5 56-57 2.2181408430709717E-5 0.0 0.0 1.3923491886040795 8.872563372283887E-5 58-59 2.2181408430709717E-5 0.0 0.0 1.5971168605321764 1.2199774636890344E-4 60-61 2.2181408430709717E-5 0.0 0.0 1.822346881737603 1.330884505842583E-4 62-63 2.2181408430709717E-5 0.0 0.0 2.0490630573078867 1.5526985901496802E-4 64-65 2.2181408430709717E-5 0.0 0.0 2.2849734266726998 1.5526985901496802E-4 66-67 2.2181408430709717E-5 0.0 0.0 2.558070927271598 1.5526985901496802E-4 68-69 2.2181408430709717E-5 0.0 0.0 2.8586511929161453 1.7745126744567774E-4 70-71 2.2181408430709717E-5 0.0 0.0 3.174115183617699 1.9963267587638745E-4 72-73 3.327211264606458E-5 0.0 0.0 3.4960450548768045 1.9963267587638745E-4 74-75 4.4362816861419435E-5 0.0 0.0 3.829143265280772 1.9963267587638745E-4 76-77 4.4362816861419435E-5 1.1090704215354859E-5 0.0 4.181472756794165 1.9963267587638745E-4 78-79 4.4362816861419435E-5 2.2181408430709717E-5 0.0 4.572719529399238 1.9963267587638745E-4 80-81 4.4362816861419435E-5 2.2181408430709717E-5 0.0 4.988909295784645 1.9963267587638745E-4 82-83 4.4362816861419435E-5 2.2181408430709717E-5 0.0 5.408481726955735 2.4399549273780688E-4 84-85 4.4362816861419435E-5 2.2181408430709717E-5 0.0 5.832922977277365 2.5508619695316175E-4 86-87 4.4362816861419435E-5 2.2181408430709717E-5 0.0 6.2692867346305015 2.661769011685166E-4 88-89 4.4362816861419435E-5 2.2181408430709717E-5 0.0 6.7310592953410175 2.661769011685166E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19440 0.0 26.103476 1 GTACATG 15550 0.0 24.352476 1 GGTATCA 14255 0.0 23.921686 1 TACATGG 15465 0.0 23.726452 2 ACATGGG 15920 0.0 22.540796 3 ATCAACG 22690 0.0 22.141441 3 TATCAAC 22955 0.0 21.98936 2 TCAACGC 23065 0.0 21.781458 4 CAACGCA 23170 0.0 21.600214 5 AACGCAG 23975 0.0 20.929546 6 GAGTACT 11095 0.0 19.758955 12-13 AGAGTAC 19150 0.0 19.67078 10-11 GTGTAAG 1865 0.0 19.179417 1 AGTACTT 11810 0.0 19.085604 12-13 ACGCAGA 26960 0.0 18.555449 7 GTACTTT 11590 0.0 18.526522 14-15 CGCAGAG 26905 0.0 18.399145 8 CATGGGA 7700 0.0 17.90081 4 CATGGGG 8820 0.0 17.621569 4 TAAGGTG 2145 0.0 17.504736 5 >>END_MODULE