FastQCFastQC Report
Thu 26 May 2016
SRR938548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508280
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103650.2299102983843062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81110.17991340378148651No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67400.14950269282298348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56110.12445988270471221No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA192800.026.5186021
GGTATCA141000.025.5106871
GTACATG161750.024.2650491
TACATGG160500.023.5307792
ATCAACG225650.022.3755993
ACATGGG161800.022.3399873
TCAACGC227650.022.1787764
CAACGCA229150.022.0543235
TATCAAC230200.021.957382
AACGCAG235400.021.4889456
GAGTACT114350.020.2285512-13
AGTACTT121550.019.9095412-13
AGAGTAC200550.019.76403410-11
GTACTTT118000.019.36132214-15
GTAAGGT20650.018.8610464
CATGGGG85450.018.787834
ACGCAGA270550.018.6444237
CGCAGAG269150.018.6002248
TAAGGTG22550.018.535665
GGTAAGG20850.017.5412863
Produced by FastQC (version 0.11.5)