##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938548_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4508280 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.823328187246577 31.0 31.0 34.0 30.0 34.0 2 32.08171142874888 33.0 31.0 34.0 30.0 34.0 3 32.145622277232114 33.0 31.0 34.0 30.0 34.0 4 35.79048351034097 37.0 35.0 37.0 35.0 37.0 5 35.683008597513904 37.0 35.0 37.0 33.0 37.0 6 35.71117100091387 37.0 35.0 37.0 33.0 37.0 7 35.633518769907816 37.0 35.0 37.0 33.0 37.0 8 35.60524989574738 37.0 35.0 37.0 33.0 37.0 9 37.1512084431313 39.0 37.0 39.0 33.0 39.0 10-11 37.082364782134206 39.0 37.0 39.0 33.0 39.0 12-13 36.96472002626278 39.0 37.0 39.0 33.0 39.0 14-15 37.99756902854304 40.0 37.0 41.0 33.0 41.0 16-17 38.090006831873794 40.0 37.0 41.0 33.0 41.0 18-19 38.217315361956224 40.0 37.5 41.0 33.0 41.0 20-21 38.16827071521733 40.0 37.5 41.0 33.0 41.0 22-23 38.04196999742696 40.0 37.0 41.0 33.0 41.0 24-25 37.92333794706629 40.0 37.0 41.0 32.5 41.0 26-27 37.71479211584019 40.0 37.0 41.0 32.0 41.0 28-29 37.58584537783811 39.5 37.0 41.0 32.0 41.0 30-31 37.35196460734471 39.0 36.0 41.0 31.5 41.0 32-33 37.265355856335454 39.0 36.0 41.0 31.0 41.0 34-35 37.14333149227643 39.0 36.0 41.0 31.0 41.0 36-37 37.02850410799684 39.0 36.0 41.0 31.0 41.0 38-39 36.8300128873983 39.0 35.5 41.0 30.0 41.0 40-41 36.62900895685273 39.0 35.0 40.0 30.0 41.0 42-43 36.381189278394416 39.0 35.0 40.0 30.0 41.0 44-45 36.11292943206722 38.0 35.0 40.0 29.5 41.0 46-47 35.72914182348922 38.0 35.0 40.0 28.5 41.0 48-49 35.34325252646242 38.0 34.0 40.0 27.0 41.0 50-51 34.95898923758063 38.0 34.0 40.0 26.0 41.0 52-53 34.37890004613733 38.0 33.0 40.0 24.0 41.0 54-55 33.965369497901634 37.0 33.0 40.0 24.0 41.0 56-57 33.57663321710275 37.0 32.0 39.5 23.0 41.0 58-59 33.05215148571074 36.0 31.5 39.0 21.5 40.0 60-61 32.56752675077857 36.0 31.0 39.0 20.0 40.0 62-63 32.07620733406088 35.0 30.5 39.0 18.5 40.0 64-65 31.435360159528692 35.0 29.5 38.0 15.0 40.0 66-67 30.788703230500325 34.0 29.0 38.0 10.0 40.0 68-69 30.07460128918346 34.0 28.0 37.0 7.5 39.0 70-71 29.285111173219057 33.5 27.0 36.5 6.0 39.0 72-73 28.71393990612828 33.0 26.0 36.0 2.0 39.0 74-75 28.003546252672862 32.5 26.0 35.0 2.0 38.0 76-77 26.83131415972389 31.0 25.0 34.0 2.0 36.0 78-79 27.34582224262912 32.0 26.0 35.0 2.0 37.0 80-81 26.968014409042915 32.0 25.0 35.0 2.0 36.0 82-83 26.291934507173465 32.0 23.5 34.5 2.0 36.0 84-85 25.51847134605659 31.0 21.0 34.0 2.0 35.5 86-87 24.62886499951201 31.0 18.5 34.0 2.0 35.0 88-89 23.666855541359453 30.0 10.0 34.0 2.0 35.0 90-91 22.813888112539594 29.5 4.5 34.0 2.0 35.0 92-93 21.735269326661168 29.0 2.0 34.0 2.0 35.0 94-95 20.5704175428323 28.0 2.0 33.0 2.0 35.0 96-97 19.192157430328194 25.5 2.0 33.0 2.0 34.0 98-99 17.414196434116782 22.0 2.0 32.0 2.0 34.0 100-101 13.068152931938567 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23517280323534706 1101 2 0.21348334232188648 1101 3 0.19082759481468514 1101 4 0.14102927726929693 1101 5 0.13752454383001123 1101 6 0.16026135133039787 1101 7 0.19427200293502267 1101 8 0.23802870420612265 1101 9 0.19550469052144592 1101 10-11 0.24981302961192142 1101 12-13 0.30829916755247666 1101 14-15 0.3311592260069318 1101 16-17 0.3115036908640647 1101 18-19 0.34565466801122113 1101 20-21 0.2984538457245307 1101 22-23 0.3266862531875674 1101 24-25 0.302682475187396 1101 26-27 0.4347133354315531 1101 28-29 0.469893540830455 1101 30-31 0.4517862661537535 1101 32-33 0.46985179802514665 1101 34-35 0.2999331145263895 1101 36-37 0.38746346219136996 1101 38-39 0.5575595749906057 1101 40-41 0.4889814372982997 1101 42-43 0.4779964213103014 1101 44-45 0.5884663980474798 1101 46-47 0.5379578399551193 1101 48-49 0.5962400770520659 1101 50-51 0.861820531630535 1101 52-53 0.7970622657109132 1101 54-55 0.8060134469097164 1101 56-57 0.9156583767474729 1101 58-59 0.917287040834573 1101 60-61 0.9153308198033727 1101 62-63 0.9715829650828738 1101 64-65 0.9918266598571144 1101 66-67 1.2424786553208946 1101 68-69 1.44258756572642 1101 70-71 1.5161447176384861 1101 72-73 1.5070591910579019 1101 74-75 1.5858332610497925 1101 76-77 1.6295540064013707 1101 78-79 1.6391800888162997 1101 80-81 1.8337543785590285 1101 82-83 1.9083250910351914 1101 84-85 2.0493221717843717 1101 86-87 2.067806964078301 1101 88-89 2.004945459917465 1101 90-91 2.128720790460992 1101 92-93 2.585127440705275 1101 94-95 2.49384986388354 1101 96-97 2.5667440992981554 1101 98-99 2.5879069275584534 1101 100-101 2.25332175118926 1102 1 0.2593804801960182 1102 2 0.25098955870906536 1102 3 0.2808315038983764 1102 4 0.19897697770998946 1102 5 0.12438292910797344 1102 6 0.2165739091086678 1102 7 0.2578268271959345 1102 8 0.2037856923949235 1102 9 0.33793076431381763 1102 10-11 0.3054587530820072 1102 12-13 0.3859723671349329 1102 14-15 0.47499022243797384 1102 16-17 0.3544242241937283 1102 18-19 0.45778805426517977 1102 20-21 0.3643942763412298 1102 22-23 0.41891144422107374 1102 24-25 0.32347333997520167 1102 26-27 0.4785764057412152 1102 28-29 0.5532115006459861 1102 30-31 0.4639967624007042 1102 32-33 0.39865352863672143 1102 34-35 0.445560835033028 1102 36-37 0.4356450238192764 1102 38-39 0.5421281672892491 1102 40-41 0.5956800936400839 1102 42-43 0.660168556483967 1102 44-45 0.5674213279965485 1102 46-47 0.4946384882302155 1102 48-49 0.6688715601793476 1102 50-51 0.7932309759775151 1102 52-53 0.7750878633197331 1102 54-55 0.6689113945642191 1102 56-57 0.8909219719766526 1102 58-59 1.0496537307465132 1102 60-61 0.9604241542798277 1102 62-63 1.1261099748864538 1102 64-65 1.2732571471887049 1102 66-67 1.320496706509985 1102 68-69 1.3585226188014197 1102 70-71 1.6676253468784807 1102 72-73 1.6777005499799138 1102 74-75 1.5694706391875535 1102 76-77 1.6272332112243433 1102 78-79 1.6258499092867815 1102 80-81 1.8926400868520759 1102 82-83 1.9835536780213587 1102 84-85 2.100133816975319 1102 86-87 1.9833282079834333 1102 88-89 1.9180731236505366 1102 90-91 2.08574546106583 1102 92-93 2.3312340494807593 1102 94-95 2.362106279871284 1102 96-97 2.6620139804461935 1102 98-99 2.863529637956848 1102 100-101 2.35941851198268 1103 1 0.22597119678673394 1103 2 0.23641777689589816 1103 3 0.2029401932484447 1103 4 0.16514051283903797 1103 5 0.146522261592132 1103 6 0.1599462059292449 1103 7 0.2144588269313843 1103 8 0.2520772924106609 1103 9 0.3167084930915465 1103 10-11 0.27341057879245767 1103 12-13 0.31016416201638464 1103 14-15 0.3951853753917476 1103 16-17 0.31341905818855764 1103 18-19 0.38710249651065 1103 20-21 0.32066182312601654 1103 22-23 0.3759218365762891 1103 24-25 0.3143809343310764 1103 26-27 0.4787276903752655 1103 28-29 0.4474602224809203 1103 30-31 0.3767333777235322 1103 32-33 0.4647090946922887 1103 34-35 0.41747626384501046 1103 36-37 0.41433718354591775 1103 38-39 0.37162812178920746 1103 40-41 0.37526296805054216 1103 42-43 0.45532780422941954 1103 44-45 0.619237853951013 1103 46-47 0.5683913766382673 1103 48-49 0.6431157887339225 1103 50-51 0.5977815477694648 1103 52-53 0.6204504371305859 1103 54-55 0.7260741758649374 1103 56-57 0.9392662832520031 1103 58-59 0.9340080030318205 1103 60-61 0.9022027426101431 1103 62-63 0.9947792832109315 1103 64-65 1.1456977696293258 1103 66-67 0.9556836779038562 1103 68-69 0.9359880755772174 1103 70-71 1.0483116154957877 1103 72-73 1.17429103363936 1103 74-75 1.237719760022884 1103 76-77 1.1931819366558258 1103 78-79 1.3235817621910506 1103 80-81 1.5521936300263093 1103 82-83 1.702711556661999 1103 84-85 1.8736107542453588 1103 86-87 1.688208335277352 1103 88-89 1.6798291828203844 1103 90-91 2.005403755724128 1103 92-93 2.1740380905606607 1103 94-95 2.075680898618316 1103 96-97 1.9919200862143676 1103 98-99 1.8029195644157383 1103 100-101 1.6725822001463708 1104 1 0.22862304839932435 1104 2 0.20578003047413063 1104 3 0.22701614119156233 1104 4 0.13073321431610907 1104 5 0.09263177841917525 1104 6 0.13493048739596247 1104 7 0.18750743158786065 1104 8 0.19282045463984332 1104 9 0.10353967576568834 1104 10-11 0.2556646749392044 1104 12-13 0.24699389674768213 1104 14-15 0.33675163743264136 1104 16-17 0.39141369634486267 1104 18-19 0.3434166967644927 1104 20-21 0.33906623467369457 1104 22-23 0.3058012786282305 1104 24-25 0.29750730465582365 1104 26-27 0.4161542838502754 1104 28-29 0.3036766309032686 1104 30-31 0.3601756780368959 1104 32-33 0.5175474803251277 1104 34-35 0.445092237104852 1104 36-37 0.40191233038680707 1104 38-39 0.5172191358559743 1104 40-41 0.46842940183973525 1104 42-43 0.45673874098717704 1104 44-45 0.4160088300482556 1104 46-47 0.5704173271152868 1104 48-49 0.6809400374325847 1104 50-51 0.6865662280887435 1104 52-53 0.7103921630004777 1104 54-55 0.7613712909502155 1104 56-57 0.9535982163503576 1104 58-59 0.9287046061022366 1104 60-61 0.8357206979366509 1104 62-63 0.8862810004154511 1104 64-65 1.0845808265262384 1104 66-67 1.083309981678923 1104 68-69 1.2389070447978519 1104 70-71 1.2979073609942127 1104 72-73 1.521575082711255 1104 74-75 1.6556054460498437 1104 76-77 1.5388378296996308 1104 78-79 1.6183479500527227 1104 80-81 1.835366473747058 1104 82-83 1.962944558509239 1104 84-85 2.1375791144668064 1104 86-87 2.101297794197823 1104 88-89 2.1346925721983894 1104 90-91 2.43964687980559 1104 92-93 2.5631080713085908 1104 94-95 2.4829271006921054 1104 96-97 2.7236290597287045 1104 98-99 2.8882250608624283 1104 100-101 2.2509910891788163 1105 1 0.1328539536694926 1105 2 0.1768404095599152 1105 3 0.17877945184632438 1105 4 0.11249049122350385 1105 5 0.06442296914801204 1105 6 0.08546780300256529 1105 7 0.14106006042916874 1105 8 0.1161981048073315 1105 9 0.2206885220715762 1105 10-11 0.20622201634527215 1105 12-13 0.21144784261040428 1105 14-15 0.2851378700856202 1105 16-17 0.2031854854549877 1105 18-19 0.24670196817908874 1105 20-21 0.2497170991640516 1105 22-23 0.3415340668436926 1105 24-25 0.2045043710062373 1105 26-27 0.2299778071426246 1105 28-29 0.19402731646180627 1105 30-31 0.27827353917748354 1105 32-33 0.3536084835916711 1105 34-35 0.42441468888687695 1105 36-37 0.4095689477431961 1105 38-39 0.39649503415611775 1105 40-41 0.45232423778422515 1105 42-43 0.4030989869143937 1105 44-45 0.4305844911597134 1105 46-47 0.5368306804224048 1105 48-49 0.6156933820011687 1105 50-51 0.6112239689704992 1105 52-53 0.6001379133697853 1105 54-55 0.61801049366332 1105 56-57 0.7584520020066847 1105 58-59 0.77079987689266 1105 60-61 0.8454717018273783 1105 62-63 0.8969433082197895 1105 64-65 1.1737075976391544 1105 66-67 1.0783295393428212 1105 68-69 1.1619385347173328 1105 70-71 1.2394221436752808 1105 72-73 1.420868718148082 1105 74-75 1.5100987673156823 1105 76-77 1.3493303083214379 1105 78-79 1.538988047424919 1105 80-81 1.6885484952604841 1105 82-83 1.9886337581014395 1105 84-85 2.0852314145729167 1105 86-87 2.0007581379133654 1105 88-89 1.846839389916802 1105 90-91 1.990825541145913 1105 92-93 2.0594873027340093 1105 94-95 2.0193009745659793 1105 96-97 2.146636102568312 1105 98-99 2.2882919002191073 1105 100-101 2.1279120054761744 1106 1 -0.10413973054951953 1106 2 -0.06386820574592633 1106 3 -0.02746778890527679 1106 4 0.011939750283410433 1106 5 0.022149570217642633 1106 6 -0.04459389328759755 1106 7 -0.029605324131942723 1106 8 -0.08721015912901464 1106 9 -0.10130871011249809 1106 10-11 -0.0030469163824875523 1106 12-13 -0.0520075124985695 1106 14-15 0.00136146949617455 1106 16-17 -0.11466438220188735 1106 18-19 -0.029597322832664474 1106 20-21 -0.08626221478852614 1106 22-23 0.04481417062167026 1106 24-25 -0.04158460228121896 1106 26-27 -0.2889794136718393 1106 28-29 -0.02962687588225066 1106 30-31 0.12524469079452416 1106 32-33 -0.022466741331172102 1106 34-35 -0.1662421180655187 1106 36-37 -0.14148082657800387 1106 38-39 -0.09328166075315636 1106 40-41 -0.03894048576680831 1106 42-43 0.0969301390555728 1106 44-45 0.09061676554643583 1106 46-47 -0.19723729334685203 1106 48-49 0.002585868580389672 1106 50-51 0.24040331054076347 1106 52-53 0.012987796145829122 1106 54-55 0.1290119068016793 1106 56-57 0.33749040772051586 1106 58-59 0.509538262850775 1106 60-61 0.6068062154638625 1106 62-63 0.4606200835630432 1106 64-65 0.6671180588947685 1106 66-67 0.4346887123444212 1106 68-69 0.5619325626454632 1106 70-71 0.33967135798244996 1106 72-73 0.69372577748981 1106 74-75 0.7299379144735809 1106 76-77 0.7178041188963888 1106 78-79 0.8755282069623966 1106 80-81 0.9131392445463575 1106 82-83 1.252284956849973 1106 84-85 1.2816261730593048 1106 86-87 1.336706255979962 1106 88-89 1.3185918175514146 1106 90-91 1.4253065202229962 1106 92-93 1.4432163047277236 1106 94-95 1.2593983470559422 1106 96-97 1.2318046258689712 1106 98-99 1.2646052772569192 1106 100-101 1.5654126858387922 1107 1 0.04959235302775511 1107 2 0.040107872228063 1107 3 0.06774534410845234 1107 4 0.09517668769394305 1107 5 0.008053414662022362 1107 6 -0.00570637631373927 1107 7 0.037543615902450256 1107 8 0.08703452341772788 1107 9 0.028469976471185987 1107 10-11 0.10962893353643466 1107 12-13 0.10730489401112209 1107 14-15 0.2206956881776847 1107 16-17 0.14169822764923623 1107 18-19 0.0705991744607104 1107 20-21 0.1424969723591616 1107 22-23 0.17344123085701568 1107 24-25 0.20510947096921228 1107 26-27 0.24008941704018838 1107 28-29 0.18812306032210557 1107 30-31 0.18439781413737677 1107 32-33 0.20871519561190865 1107 34-35 0.37804008225970875 1107 36-37 0.28850055486401516 1107 38-39 0.3270394698589456 1107 40-41 0.438690591295277 1107 42-43 0.3798437110906292 1107 44-45 0.34870817991696157 1107 46-47 0.3922677099176539 1107 48-49 0.2731591739917931 1107 50-51 0.43612119067851296 1107 52-53 0.3617408690652084 1107 54-55 0.42736891029913693 1107 56-57 0.45307552107369276 1107 58-59 0.6426694776578117 1107 60-61 0.7255512248435423 1107 62-63 0.8376693510434308 1107 64-65 0.875208199268176 1107 66-67 0.8413974231512888 1107 68-69 1.0006770806262573 1107 70-71 0.8289148469950369 1107 72-73 0.9966789890354057 1107 74-75 0.9535253917140523 1107 76-77 1.0641581498616084 1107 78-79 1.2454272134745317 1107 80-81 1.4631778763539103 1107 82-83 1.5880681882507695 1107 84-85 1.7380579140175705 1107 86-87 1.9910871441456237 1107 88-89 1.8633404157714253 1107 90-91 2.0645763895971037 1107 92-93 2.058005984086595 1107 94-95 2.0524690527469005 1107 96-97 1.9400746824152826 1107 98-99 2.0441043693663374 1107 100-101 1.8749206365387447 1108 1 0.10225600556366032 1108 2 0.06707615003224987 1108 3 0.09664116213706109 1108 4 0.05437527821869992 1108 5 0.06251033095777103 1108 6 0.05697219382872021 1108 7 0.06396056345585777 1108 8 0.10033144950850215 1108 9 0.14949106278263713 1108 10-11 0.0917639068161833 1108 12-13 0.07633544314469276 1108 14-15 0.2691621546225207 1108 16-17 0.22433285522222945 1108 18-19 0.1094367850148501 1108 20-21 0.12275934487980322 1108 22-23 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-0.559126846390491 2316 50-51 -0.6575310104987651 2316 52-53 -0.5672340504307485 2316 54-55 -0.30352788144648457 2316 56-57 -0.4059361105957109 2316 58-59 -0.5074671656600955 2316 60-61 -0.2436370497813698 2316 62-63 -0.2875255994634074 2316 64-65 -0.27580466973025963 2316 66-67 -0.16268545524360079 2316 68-69 0.10855581526318403 2316 70-71 -0.06565640426041242 2316 72-73 0.23048547776484085 2316 74-75 0.3611321987062581 2316 76-77 0.3182584201066945 2316 78-79 0.1593732285243874 2316 80-81 -0.1403699640151146 2316 82-83 0.4157562887953965 2316 84-85 0.4925236557937289 2316 86-87 0.5503288821269656 2316 88-89 0.5450623577826263 2316 90-91 0.7185900403389844 2316 92-93 0.6806880953633758 2316 94-95 0.8202097915462261 2316 96-97 0.7109762079798472 2316 98-99 0.5799526936656143 2316 100-101 0.09222274478691439 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 26.0 4 57.0 5 127.0 6 305.0 7 785.0 8 1888.0 9 3421.0 10 5606.0 11 8330.0 12 12588.0 13 17512.0 14 23694.0 15 31472.0 16 39660.0 17 49678.0 18 50959.0 19 49199.0 20 52332.0 21 57335.0 22 63430.0 23 71064.0 24 80659.0 25 92406.0 26 107100.0 27 125602.0 28 148024.0 29 173464.0 30 202710.0 31 236772.0 32 278587.0 33 334376.0 34 406847.0 35 495825.0 36 581703.0 37 525646.0 38 175418.0 39 3665.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.419899880258335 19.04287426148767 14.691372898003486 23.845852960250514 2 15.903523744155082 20.42303919328483 38.99704554055871 24.676391522001385 3 19.4693404309169 23.632819388487782 29.64484059162402 27.2529995889713 4 13.410968262840816 16.60458090446911 35.955597256603404 34.02885357608667 5 14.023529223874545 37.24221241603756 33.944403458003585 14.789854902084304 6 33.32230917334327 35.68059215487946 16.747140816453282 14.24995785532398 7 29.60332987303362 32.02054885677021 20.100282147515237 18.275839122680935 8 26.719952336578988 35.19440567010161 19.218264885558327 18.867377107761076 9 26.275102789011466 16.841382847144708 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22.89560049007783 34-35 23.024809267202055 26.586293592799965 27.89372403881573 22.495173101182246 36-37 23.148656888839007 26.167149524635736 28.030953188470765 22.65324039805449 38-39 22.52552628765053 26.62007016429199 28.013051427932524 22.84135212012496 40-41 22.88874205402988 26.440246426705183 28.06646036085511 22.60455115840983 42-43 22.676297612874784 26.418435960215504 27.77200722043683 23.133259206472882 44-45 22.625446210023544 26.54151380791691 27.972646574156208 22.860393407903338 46-47 22.968872257752544 26.937072972992453 27.368057977797505 22.725996791457494 48-49 22.844854356872244 26.708534075079633 27.51526080900033 22.931350759047795 50-51 22.865904513472987 26.912969025881267 27.498802203944745 22.722324256701004 52-53 23.12927546647502 26.808139689637734 27.20036244421376 22.86222239967349 54-55 22.570037797119966 26.740164763501824 27.50196305464612 23.187834384732092 56-57 22.793595649959148 26.931127495533495 27.349712900453486 22.925563954053867 58-59 22.915990133709528 26.54434728987552 27.32670774663508 23.21295482977987 60-61 22.521061247304957 26.84463919721047 27.727902880921327 22.906396674563247 62-63 22.424161764575405 26.87973018534785 27.75226915808246 22.943838891994286 64-65 22.23099620642252 26.8688207414029 27.53073651743931 23.36944653473527 66-67 22.310054895944806 26.80069335007891 27.409154736426377 23.480097017549912 68-69 22.541568965742883 26.578562633547392 27.560000807412045 23.319867593297683 70-71 22.565841928354693 26.58357286489732 27.311156575619716 23.539428631128267 72-73 22.553717614133745 26.54868287190587 27.401814153064546 23.495785360895837 74-75 22.379233321799 27.14998846566762 26.91078415715084 23.559994055382543 76-77 22.371669461524128 26.642200573167596 27.28592722723522 23.700202738073056 78-79 22.55314665459998 26.69583521875305 27.303162181585883 23.44785594506109 80-81 22.61410155612717 26.931728107565746 27.085822505745266 23.36834783056182 82-83 22.577590889903774 26.80301954492551 27.144628189916702 23.47476137525401 84-85 22.44818622104299 26.721504501201082 27.13868331078463 23.6916259669713 86-87 22.426408974920218 26.78512229226076 26.989744066950106 23.79872466586891 88-89 22.321705839033957 26.830742544828627 26.78002475445181 24.06752686168561 90-91 22.337389819135634 26.62404445220805 26.996242967695068 24.042322760961245 92-93 22.335071883062632 26.400010913302573 27.112070633910935 24.15284656972386 94-95 22.114049570828232 26.237266059435356 27.232624263396442 24.416060106339973 96-97 21.991568846655486 26.17476066260303 27.265475968662106 24.568194522079374 98-99 21.976884754274355 26.315634787546472 26.89491335941867 24.812567098760503 100-101 22.057807424961943 25.9009480114252 27.137639440682353 24.903605122930504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 651.0 1 450.5 2 361.0 3 496.5 4 440.5 5 445.0 6 843.5 7 1727.5 8 2662.5 9 3050.5 10 2688.5 11 2312.5 12 2480.0 13 2713.5 14 2969.0 15 3262.0 16 3548.0 17 3862.0 18 4192.0 19 4509.0 20 5067.0 21 6081.5 22 7344.5 23 8976.5 24 11176.5 25 14381.5 26 18874.0 27 24445.5 28 29997.5 29 36053.5 30 43492.5 31 52422.5 32 64649.0 33 71680.5 34 75218.5 35 83078.0 36 94208.0 37 104736.0 38 112344.5 39 119338.5 40 126434.0 41 137201.5 42 146920.5 43 154090.0 44 160483.5 45 168852.5 46 176729.0 47 185879.5 48 193499.0 49 190770.5 50 182396.5 51 170642.5 52 162787.0 53 163269.0 54 164985.0 55 155607.5 56 137235.5 57 121765.5 58 110246.0 59 95726.0 60 78163.5 61 65256.0 62 55265.5 63 43018.0 64 32740.5 65 25487.5 66 18570.0 67 14553.0 68 11438.0 69 7979.5 70 5956.5 71 4515.5 72 3529.0 73 2845.0 74 2202.5 75 1701.5 76 1118.0 77 610.5 78 366.0 79 236.5 80 134.0 81 79.5 82 39.5 83 19.5 84 13.5 85 9.0 86 4.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023889376879874365 2 0.011489969567107633 3 0.0017523312660260677 4 0.0 5 3.1053971802993605E-4 6 0.0 7 0.0 8 2.2181408430709717E-5 9 3.549025348913555E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015637892943650348 26-27 0.008739474921699629 28-29 1.2199774636890344E-4 30-31 0.0 32-33 3.6599323910671035E-4 34-35 0.010391989849787503 36-37 0.0011756146468276149 38-39 0.012732128439227377 40-41 0.012654493509719892 42-43 0.005378991544447107 44-45 7.319864782134207E-4 46-47 5.545352107677429E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.1090704215354859E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.545352107677429E-5 66-67 5.212630981216783E-4 68-69 0.0010868890131047761 70-71 3.327211264606457E-5 72-73 1.2199774636890344E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.981633793819371E-5 82-83 0.0011312518299661956 84-85 0.005789347600415236 86-87 0.004580460840941557 88-89 0.0 90-91 0.001497245069072906 92-93 4.5471887282954915E-4 94-95 7.763492950748401E-5 96-97 0.0 98-99 0.0 100-101 2.2181408430709717E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4508280.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.83800757428411 #Duplication Level Percentage of deduplicated Percentage of total 1 69.85854124255778 30.62459260119687 2 15.237331625925094 13.359485184583662 3 5.805178031518957 7.634623155473874 4 2.873454638957597 5.038661049079398 5 1.640154826118757 3.595055984519636 6 1.0027736469248667 2.637575923751089 7 0.6736715935391926 2.0672694294105822 8 0.470386811728676 1.6496656492324044 9 0.3556269672141471 1.4030979914118125 >10 1.8387135903285896 14.479917709690305 >50 0.14466731105272607 4.378970760439453 >100 0.0890216677081118 7.455547524519554 >500 0.006529797454823346 1.9331639519894244 >1k 0.003745774664484456 3.058316547008027 >5k 1.518557296412549E-4 0.45405536945848435 >10k+ 5.0618576547084965E-5 0.23000116823561675 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10365 0.2299102983843062 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8111 0.17991340378148651 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6740 0.14950269282298348 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5611 0.12445988270471221 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.2181408430709717E-5 0.0 4 0.0 0.0 0.0 2.2181408430709717E-5 0.0 5 0.0 0.0 0.0 2.2181408430709717E-5 0.0 6 0.0 0.0 0.0 2.2181408430709717E-5 0.0 7 0.0 0.0 0.0 2.2181408430709717E-5 0.0 8 0.0 0.0 0.0 2.2181408430709717E-5 0.0 9 0.0 0.0 0.0 6.654422529212916E-5 0.0 10-11 0.0 0.0 0.0 7.763492950748401E-5 0.0 12-13 0.0 0.0 0.0 9.981633793819373E-5 3.327211264606458E-5 14-15 0.0 0.0 0.0 1.1090704215354858E-4 5.54535210767743E-5 16-17 0.0 0.0 0.0 1.330884505842583E-4 6.654422529212916E-5 18-19 0.0 0.0 0.0 1.5526985901496802E-4 6.654422529212916E-5 20-21 0.0 0.0 0.0 2.2181408430709717E-4 6.654422529212916E-5 22-23 0.0 0.0 0.0 3.438118306760006E-4 6.654422529212916E-5 24-25 0.0 0.0 0.0 5.434445065523881E-4 6.654422529212916E-5 26-27 0.0 0.0 0.0 0.0012088867594736794 6.654422529212916E-5 28-29 0.0 0.0 0.0 0.003571206757344264 6.654422529212916E-5 30-31 0.0 0.0 0.0 0.01246595153805886 6.654422529212916E-5 32-33 0.0 0.0 0.0 0.031031790394562893 6.654422529212916E-5 34-35 0.0 0.0 0.0 0.05963471656596307 6.654422529212916E-5 36-37 0.0 0.0 0.0 0.10989778806995129 6.654422529212916E-5 38-39 0.0 0.0 0.0 0.18693381954980615 6.654422529212916E-5 40-41 0.0 0.0 0.0 0.2723433327122539 6.654422529212916E-5 42-43 0.0 0.0 0.0 0.3612796898151845 6.654422529212916E-5 44-45 0.0 0.0 0.0 0.47163219675796536 6.654422529212916E-5 46-47 0.0 0.0 0.0 0.6019368805841696 6.654422529212916E-5 48-49 0.0 0.0 0.0 0.7407370438393357 8.872563372283887E-5 50-51 0.0 0.0 0.0 0.8929347777866503 8.872563372283887E-5 52-53 0.0 0.0 0.0 1.0418274818777893 8.872563372283887E-5 54-55 0.0 0.0 0.0 1.2031417746901258 8.872563372283887E-5 56-57 0.0 0.0 0.0 1.3960978466288696 9.981633793819373E-5 58-59 0.0 0.0 0.0 1.600510616022075 1.1090704215354858E-4 60-61 0.0 0.0 0.0 1.8256851837064247 1.1090704215354858E-4 62-63 0.0 0.0 0.0 2.052534447727293 1.1090704215354858E-4 64-65 0.0 0.0 0.0 2.289243347795612 1.1090704215354858E-4 66-67 0.0 0.0 0.0 2.5634277374076144 1.1090704215354858E-4 68-69 0.0 0.0 0.0 2.866115236853079 1.1090704215354858E-4 70-71 0.0 0.0 0.0 3.184706806143363 1.1090704215354858E-4 72-73 0.0 0.0 0.0 3.5123262086649456 1.330884505842583E-4 74-75 0.0 0.0 0.0 3.853165730611231 1.7745126744567774E-4 76-77 0.0 0.0 0.0 4.214656143806508 1.7745126744567774E-4 78-79 0.0 0.0 0.0 4.618801405414038 1.7745126744567774E-4 80-81 0.0 0.0 0.0 5.05092851375691 1.7745126744567774E-4 82-83 0.0 0.0 0.0 5.489643500403702 1.9963267587638745E-4 84-85 0.0 2.2181408430709717E-5 0.0 5.935401084227244 1.9963267587638745E-4 86-87 0.0 2.2181408430709717E-5 0.0 6.395476767192809 1.9963267587638745E-4 88-89 0.0 2.2181408430709717E-5 0.0 6.8919188692805236 1.9963267587638745E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19280 0.0 26.518602 1 GGTATCA 14100 0.0 25.510687 1 GTACATG 16175 0.0 24.265049 1 TACATGG 16050 0.0 23.530779 2 ATCAACG 22565 0.0 22.375599 3 ACATGGG 16180 0.0 22.339987 3 TCAACGC 22765 0.0 22.178776 4 CAACGCA 22915 0.0 22.054323 5 TATCAAC 23020 0.0 21.95738 2 AACGCAG 23540 0.0 21.488945 6 GAGTACT 11435 0.0 20.22855 12-13 AGTACTT 12155 0.0 19.90954 12-13 AGAGTAC 20055 0.0 19.764034 10-11 GTACTTT 11800 0.0 19.361322 14-15 GTAAGGT 2065 0.0 18.861046 4 CATGGGG 8545 0.0 18.78783 4 ACGCAGA 27055 0.0 18.644423 7 CGCAGAG 26915 0.0 18.600224 8 TAAGGTG 2255 0.0 18.53566 5 GGTAAGG 2085 0.0 17.541286 3 >>END_MODULE