FastQCFastQC Report
Thu 26 May 2016
SRR938547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910666
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95690.5008201328751336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67200.3517098226482284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.276866809793025No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23290.12189466918864941No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23000.12037687382305438No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21250.11121776385825674No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40700.060.6536871
GTATCAA82250.046.8417781
ATCAACG109650.034.7453543
TCAACGC111100.034.291884
TATCAAC112300.034.0948682
CAACGCA113000.033.6732035
AACGCAG117850.032.3779346
AGGTAAG7600.028.1625392
ACGCAGA134700.028.1197037
CGCAGAG135550.027.7680688
TGGTATC17550.027.372672
GTACATG105450.026.2971
TACATGG104750.026.0179712
GGTAAGG9600.025.7635083
GCAGAGT146050.024.9907729
ACATGGG107000.024.4040243
GTAAGGT9750.024.3914874
TAAGGTG11050.023.674095
GTGGTAT23100.022.8557361
TAGATCG1050.002179786822.6492375
Produced by FastQC (version 0.11.5)