##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938547_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1910666 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.185389283108613 31.0 31.0 34.0 28.0 34.0 2 31.21625286680142 31.0 31.0 34.0 28.0 34.0 3 31.24701439184033 31.0 31.0 34.0 27.0 34.0 4 34.929196416328125 37.0 35.0 37.0 32.0 37.0 5 34.917067137846175 37.0 35.0 37.0 32.0 37.0 6 34.945621055694716 37.0 35.0 37.0 32.0 37.0 7 34.85846034838114 37.0 35.0 37.0 32.0 37.0 8 34.830422480956905 37.0 35.0 37.0 32.0 37.0 9 36.3253226885285 39.0 35.0 39.0 32.0 39.0 10-11 36.16130134727891 39.0 35.0 39.0 31.0 39.0 12-13 36.03528481691724 38.0 35.0 39.0 30.0 39.0 14-15 36.990218855624164 39.0 36.0 41.0 31.0 41.0 16-17 36.84087119360474 39.0 36.0 41.0 31.0 41.0 18-19 37.01524311418113 39.0 36.0 41.0 31.0 41.0 20-21 37.004552077652505 39.0 36.0 41.0 31.0 41.0 22-23 36.92192041937209 39.0 36.0 41.0 30.5 41.0 24-25 36.78618450320464 39.0 36.0 41.0 30.0 41.0 26-27 36.57691925223979 39.0 36.0 41.0 30.0 41.0 28-29 36.37981834606363 39.0 35.5 40.5 30.0 41.0 30-31 36.10683683071767 38.5 35.0 40.0 29.0 41.0 32-33 35.826085773233004 38.0 35.0 40.0 28.0 41.0 34-35 35.573449258007415 38.0 34.5 40.0 27.0 41.0 36-37 35.28716269614888 38.0 34.0 40.0 26.5 41.0 38-39 35.14513263961362 38.0 34.0 40.0 25.5 41.0 40-41 35.03764446533303 38.0 34.0 40.0 25.0 41.0 42-43 34.73070489557044 38.0 33.5 40.0 24.5 41.0 44-45 34.36162940042896 38.0 33.0 40.0 23.5 41.0 46-47 33.93287785515626 38.0 33.0 40.0 22.5 41.0 48-49 33.534052524093696 38.0 32.5 40.0 19.5 41.0 50-51 32.81041976986035 36.5 31.5 39.5 18.5 40.5 52-53 32.8241497990753 37.0 31.5 39.0 18.0 40.0 54-55 32.93957290285168 37.0 32.0 40.0 17.5 41.0 56-57 32.63682270998699 37.0 31.5 40.0 14.0 41.0 58-59 32.27110415949203 36.0 31.0 39.0 11.0 41.0 60-61 31.822630433576563 36.0 31.0 39.0 8.0 40.5 62-63 31.275556010312634 35.0 30.0 39.0 7.0 40.0 64-65 30.77614690374979 35.0 29.0 38.0 2.0 40.0 66-67 30.259151259299117 35.0 29.0 38.0 2.0 40.0 68-69 29.713900545673603 34.0 28.0 37.5 2.0 39.5 70-71 29.116130448754518 34.0 27.5 37.0 2.0 39.0 72-73 28.506924287133387 34.0 26.0 36.0 2.0 39.0 74-75 27.871703636323666 33.0 26.0 35.5 2.0 38.5 76-77 27.166290707010013 33.0 24.5 35.0 2.0 37.5 78-79 26.424606393791485 32.0 23.0 35.0 2.0 37.0 80-81 25.768331566061256 32.0 20.5 35.0 2.0 36.0 82-83 25.07316741910936 31.0 18.5 34.5 2.0 36.0 84-85 24.392692652719 31.0 15.0 34.0 2.0 35.0 86-87 23.660176346886374 30.5 7.5 34.0 2.0 35.0 88-89 22.855593808651015 30.0 2.0 34.0 2.0 35.0 90-91 21.96600033705525 29.0 2.0 34.0 2.0 35.0 92-93 21.049986496855023 29.0 2.0 34.0 2.0 35.0 94-95 20.06024522339331 27.0 2.0 33.5 2.0 35.0 96-97 18.823073211121148 25.5 2.0 33.0 2.0 34.5 98-99 17.320293290402404 21.0 2.0 32.5 2.0 34.0 100-101 13.75181899923901 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43244036186468904 1101 2 0.2644248774813036 1101 3 0.2745136643208923 1101 4 0.2551227123268234 1101 5 0.12666923729366175 1101 6 0.3800930338448012 1101 7 0.4428928924382518 1101 8 0.39902525907859143 1101 9 0.6985931730770716 1101 10-11 0.5258848689001994 1101 12-13 0.6126833382659171 1101 14-15 0.7481243313976691 1101 16-17 0.5017055225593126 1101 18-19 0.5950138135078831 1101 20-21 0.7102957667122212 1101 22-23 0.6848884030014091 1101 24-25 0.5790843598801558 1101 26-27 0.7515841656609439 1101 28-29 0.812293503601289 1101 30-31 0.7867398737430662 1101 32-33 0.790157686249735 1101 34-35 0.760014002613012 1101 36-37 0.8499465512392064 1101 38-39 0.6399575757622742 1101 40-41 0.8324937032647526 1101 42-43 0.8306435306599482 1101 44-45 0.903145252926123 1101 46-47 0.8365278928025788 1101 48-49 0.798064676940605 1101 50-51 0.9426814430262809 1101 52-53 0.8572017927564133 1101 54-55 0.9612384991586893 1101 56-57 0.9059932251417493 1101 58-59 1.002936559314655 1101 60-61 0.837432989298275 1101 62-63 0.7285211172962285 1101 64-65 0.6025356530666137 1101 66-67 0.8251399772019177 1101 68-69 0.7361404220646968 1101 70-71 0.7771134533069386 1101 72-73 0.8859305262287407 1101 74-75 0.927107592426772 1101 76-77 0.9917628043737281 1101 78-79 1.0623200493500384 1101 80-81 1.1530626372281922 1101 82-83 1.1514103221310066 1101 84-85 1.2047439238954283 1101 86-87 1.327707023133602 1101 88-89 1.1747642335113504 1101 90-91 0.876854937778571 1101 92-93 0.841118487444426 1101 94-95 0.8162690738670726 1101 96-97 0.7647367387359516 1101 98-99 0.98069746370345 1101 100-101 1.044215258991688 1102 1 0.4342519560675875 1102 2 0.3922043805868931 1102 3 0.3779556933063972 1102 4 0.269853561188107 1102 5 0.2759549515793793 1102 6 0.18153713322368503 1102 7 0.29859165020222633 1102 8 0.28663913071419955 1102 9 0.6422474173834871 1102 10-11 0.7268424258360113 1102 12-13 0.6588194666303124 1102 14-15 0.7130958634887676 1102 16-17 0.8518711540292898 1102 18-19 0.6182177472552937 1102 20-21 0.6179510462153317 1102 22-23 0.616930328467248 1102 24-25 0.7105750431099764 1102 26-27 0.6733000041702581 1102 28-29 0.45516876240045434 1102 30-31 0.6792942215691511 1102 32-33 0.8265112059184716 1102 34-35 0.740798164103694 1102 36-37 0.8967141495828841 1102 38-39 0.8311894598202443 1102 40-41 1.0540568502626826 1102 42-43 0.888529141425991 1102 44-45 0.9407824164872025 1102 46-47 1.0475113296555847 1102 48-49 1.2679197494043635 1102 50-51 1.1286804078295987 1102 52-53 1.0773234283671798 1102 54-55 0.9973228676887018 1102 56-57 1.1919714860113118 1102 58-59 1.2574500168715943 1102 60-61 1.181858455136787 1102 62-63 1.2446158377931233 1102 64-65 1.3113891727353497 1102 66-67 1.0007766231646507 1102 68-69 1.261065370304859 1102 70-71 1.3486998922303357 1102 72-73 1.1614118926883705 1102 74-75 1.1218539692383622 1102 76-77 1.43415669671327 1102 78-79 1.2603247077351334 1102 80-81 1.3324984550335763 1102 82-83 1.3125930343463281 1102 84-85 1.3345058286573348 1102 86-87 1.500734973444164 1102 88-89 1.4473988090807062 1102 90-91 1.5258715009255681 1102 92-93 1.729645920156642 1102 94-95 1.7685180386703863 1102 96-97 1.803934461303239 1102 98-99 1.9575452897904064 1102 100-101 2.0086743687225344 1103 1 0.31982050850879773 1103 2 0.2978901502232283 1103 3 0.2413706863175058 1103 4 0.09222487975894467 1103 5 0.17619183821220474 1103 6 0.466922257920217 1103 7 0.313262927084935 1103 8 0.38011763680802346 1103 9 0.3519833108580741 1103 10-11 0.4357440663862988 1103 12-13 0.45314693910388115 1103 14-15 0.4775604163223335 1103 16-17 0.6827022190382408 1103 18-19 0.7459756618682079 1103 20-21 0.5846280123008114 1103 22-23 0.6133314172629412 1103 24-25 0.6952514697020931 1103 26-27 0.6987454540341602 1103 28-29 0.78647114843249 1103 30-31 0.8254066814136749 1103 32-33 0.802829408915116 1103 34-35 0.7439934361800056 1103 36-37 0.764701103652385 1103 38-39 0.9421431369579878 1103 40-41 0.9924707397646344 1103 42-43 0.7809512214181424 1103 44-45 0.9045044302688083 1103 46-47 0.4836735700244006 1103 48-49 0.6730223757561689 1103 50-51 0.7781127136732948 1103 52-53 0.9512045926919512 1103 54-55 0.950846004597409 1103 56-57 0.8728925483227954 1103 58-59 0.7570861040733448 1103 60-61 0.9263087849634069 1103 62-63 0.914802943901659 1103 64-65 1.1014806413834286 1103 66-67 1.1234731091010417 1103 68-69 1.2806548648976666 1103 70-71 1.3406060203845342 1103 72-73 1.2528177633570934 1103 74-75 1.477156943808609 1103 76-77 1.292101717434722 1103 78-79 1.2920925294080519 1103 80-81 1.3583392064007036 1103 82-83 1.5648807314453208 1103 84-85 1.7420072386968144 1103 86-87 1.6444834714455823 1103 88-89 1.7140811700998562 1103 90-91 1.810758329930696 1103 92-93 1.9705414791453322 1103 94-95 1.8816390077966538 1103 96-97 2.0897075307752715 1103 98-99 2.2545059803419143 1103 100-101 1.6805172290182746 1104 1 0.32965352119041214 1104 2 0.2922748347562205 1104 3 0.37196472467978126 1104 4 0.17851469554751986 1104 5 0.2907238477185885 1104 6 0.30461820280308416 1104 7 0.23532569753418642 1104 8 0.3033608840300417 1104 9 0.3327635296373117 1104 10-11 0.3978096339122388 1104 12-13 0.44467520107955494 1104 14-15 0.5207370673566558 1104 16-17 0.5393851621149679 1104 18-19 0.6169793615541792 1104 20-21 0.615874303183908 1104 22-23 0.6076700972451761 1104 24-25 0.5665969832002844 1104 26-27 0.5596635760547883 1104 28-29 0.5424501298733233 1104 30-31 0.6545586611275169 1104 32-33 0.680591637154734 1104 34-35 0.5466803779722866 1104 36-37 0.6605040164765228 1104 38-39 0.7507922685300841 1104 40-41 0.5902754946109781 1104 42-43 0.7116002812231343 1104 44-45 0.8075852630247837 1104 46-47 0.9805250185696863 1104 48-49 0.793931996135818 1104 50-51 0.8984891413095184 1104 52-53 0.9573149171252453 1104 54-55 1.1523210945133044 1104 56-57 0.9839907781596864 1104 58-59 1.0297951004472594 1104 60-61 1.0056620345868588 1104 62-63 1.0182423361263382 1104 64-65 1.1365190873865068 1104 66-67 0.9564098832067351 1104 68-69 1.1624323703284993 1104 70-71 1.3726831534545383 1104 72-73 1.2797635488341967 1104 74-75 1.386711996994471 1104 76-77 1.3535486157619054 1104 78-79 1.4667998714583277 1104 80-81 1.4879156240999407 1104 82-83 1.591672401677343 1104 84-85 1.6996499287117075 1104 86-87 1.6156381580671848 1104 88-89 1.6678806399434158 1104 90-91 1.7296495466498527 1104 92-93 1.733999420404885 1104 94-95 1.7186747873759671 1104 96-97 1.9941422774604156 1104 98-99 1.9882491222135101 1104 100-101 2.001698460265672 1105 1 0.4147779437375867 1105 2 0.3154803740293666 1105 3 0.3006399698616953 1105 4 0.19763023578230587 1105 5 0.3174773449233328 1105 6 0.5134108777109745 1105 7 0.36678871470768115 1105 8 0.3514792332333059 1105 9 0.5069265064104016 1105 10-11 0.4463995617661922 1105 12-13 0.5302583515426065 1105 14-15 0.7198173301585129 1105 16-17 0.590243316859187 1105 18-19 0.5708790108879498 1105 20-21 0.6124753560200631 1105 22-23 0.5905741438404917 1105 24-25 0.6432107539322018 1105 26-27 0.6933714288933785 1105 28-29 0.6210837762159045 1105 30-31 0.643613877991605 1105 32-33 0.6164289289475562 1105 34-35 0.5767437335388408 1105 36-37 0.5700485604443699 1105 38-39 0.6936086644505295 1105 40-41 0.817451217869106 1105 42-43 0.8131371135949834 1105 44-45 0.8041022807186522 1105 46-47 0.6188389213032011 1105 48-49 0.48064566472604753 1105 50-51 0.6093857269703449 1105 52-53 0.6888899233990031 1105 54-55 0.7581682654890116 1105 56-57 0.8153687783369961 1105 58-59 0.8618053856556074 1105 60-61 0.9472245453258914 1105 62-63 0.9458040434778532 1105 64-65 1.0500872108644543 1105 66-67 1.0646757357432506 1105 68-69 0.9137658867487097 1105 70-71 1.014344600412624 1105 72-73 0.8997050880980986 1105 74-75 1.0442234536411448 1105 76-77 1.090916415632357 1105 78-79 1.0678818744518281 1105 80-81 1.0980197092763433 1105 82-83 1.1954966648465302 1105 84-85 1.0765840725943114 1105 86-87 1.2228632454738424 1105 88-89 1.4099009833785807 1105 90-91 1.3902776674652415 1105 92-93 1.8029889512433286 1105 94-95 1.9618379798681325 1105 96-97 1.8532546180066198 1105 98-99 1.8008448349195945 1105 100-101 1.813418879101441 1106 1 0.3029817631385754 1106 2 0.1580744073569491 1106 3 0.09173765582832161 1106 4 0.10625252731014712 1106 5 0.2292245906451953 1106 6 0.15646928498220092 1106 7 0.25837287423988897 1106 8 0.27599295698578175 1106 9 0.1391679380148929 1106 10-11 0.06071577578524057 1106 12-13 0.3117904811230616 1106 14-15 0.6644782137149292 1106 16-17 0.342378861958764 1106 18-19 0.14088506009569102 1106 20-21 0.36186916677288394 1106 22-23 0.3708055246271229 1106 24-25 0.036280295579281585 1106 26-27 0.0711265526794378 1106 28-29 0.25269576323125165 1106 30-31 0.0022786210891396763 1106 32-33 0.42111196286484187 1106 34-35 0.07823700837582237 1106 36-37 0.22032302985612517 1106 38-39 0.2565711638726711 1106 40-41 0.47647307845431897 1106 42-43 0.15969456190047282 1106 44-45 0.28833064874050507 1106 46-47 0.4340635312617849 1106 48-49 0.806504167386457 1106 50-51 0.4615660350796702 1106 52-53 0.46405840482799476 1106 54-55 0.6245327818399105 1106 56-57 -0.11935178547396674 1106 58-59 0.03076488809839617 1106 60-61 0.6901437869931435 1106 62-63 0.48969984037782055 1106 64-65 0.6334633904035876 1106 66-67 0.2505001531194182 1106 68-69 0.11986994890727587 1106 70-71 0.10738908266696612 1106 72-73 0.6205187882851568 1106 74-75 0.8215608832972556 1106 76-77 0.8221521734971766 1106 78-79 1.0519147566597127 1106 80-81 0.7570018243680181 1106 82-83 0.6809655996560267 1106 84-85 0.5648667628399231 1106 86-87 0.41049819692796063 1106 88-89 0.5519594109453791 1106 90-91 0.5928012526102364 1106 92-93 0.2937288150149442 1106 94-95 0.39411067228988017 1106 96-97 0.5765694654572115 1106 98-99 0.8416051070155532 1106 100-101 0.4627415707902909 1107 1 0.19057577337714093 1107 2 0.06842837645635313 1107 3 0.12650319095786955 1107 4 0.05142876072342517 1107 5 0.1165817040121695 1107 6 0.2986861984733409 1107 7 0.1613205331630212 1107 8 0.04478554983516858 1107 9 0.3172749901878831 1107 10-11 0.34313440354829794 1107 12-13 0.29923481561093723 1107 14-15 0.38002140616028157 1107 16-17 0.5475538767451198 1107 18-19 0.5204152531535087 1107 20-21 0.5899101136880276 1107 22-23 0.6392713993964065 1107 24-25 0.6045844659097099 1107 26-27 0.4688836225540385 1107 28-29 0.5060732507797283 1107 30-31 0.6176187703289102 1107 32-33 0.6590570111949532 1107 34-35 0.6639478460662716 1107 36-37 0.7556793626715432 1107 38-39 0.7565706386024473 1107 40-41 0.77782310172379 1107 42-43 0.8082702654211431 1107 44-45 0.774909731849803 1107 46-47 0.6561357012896565 1107 48-49 0.6840617081132834 1107 50-51 0.7538996636148667 1107 52-53 0.8351560410069254 1107 54-55 0.6972297870642521 1107 56-57 0.7043500616913079 1107 58-59 0.6327697394942007 1107 60-61 0.6300842581185186 1107 62-63 0.8448916375478781 1107 64-65 0.7441197050446569 1107 66-67 0.6922669888180444 1107 68-69 0.9458823284759497 1107 70-71 0.8465173021794925 1107 72-73 0.9466103523402865 1107 74-75 0.6597667545577259 1107 76-77 1.0201332185958236 1107 78-79 0.8539665409501431 1107 80-81 0.9421949031974215 1107 82-83 0.814764007246815 1107 84-85 0.8292279134338472 1107 86-87 0.8253304886504367 1107 88-89 0.7830106815215281 1107 90-91 0.6921143567366563 1107 92-93 0.9637700802438403 1107 94-95 1.0177221204493883 1107 96-97 1.2231575464454778 1107 98-99 0.8047264097697528 1107 100-101 0.8946683880414259 1108 1 0.3948039218982764 1108 2 0.25835704732657305 1108 3 0.36747126297748167 1108 4 0.25915593770766066 1108 5 0.2125124662729121 1108 6 0.33670962254576864 1108 7 0.2685241147004547 1108 8 0.3061156393112867 1108 9 0.3878189137511754 1108 10-11 0.40630798273010527 1108 12-13 0.4000146624832652 1108 14-15 0.5233904642991902 1108 16-17 0.4335095287965629 1108 18-19 0.5260036779675161 1108 20-21 0.4772158218879454 1108 22-23 0.512994286412706 1108 24-25 0.7842121036728855 1108 26-27 0.6860397552015414 1108 28-29 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-1.4726704154528427 2316 62-63 -1.7285517514978679 2316 64-65 -1.9090636683565627 2316 66-67 -1.7091359737765686 2316 68-69 -1.7852747989852098 2316 70-71 -1.7877212447317667 2316 72-73 -1.6255710272698671 2316 74-75 -1.9126390470536734 2316 76-77 -1.9987952414544807 2316 78-79 -2.3356451641481364 2316 80-81 -2.121859418136772 2316 82-83 -2.08429250113695 2316 84-85 -2.1164079305935353 2316 86-87 -2.26994304051669 2316 88-89 -2.6584102438010966 2316 90-91 -2.443597842336647 2316 92-93 -2.7509463980929354 2316 94-95 -3.1515884735469974 2316 96-97 -3.276126477869223 2316 98-99 -3.477260171094919 2316 100-101 -3.1091184249576536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6261.0 3 1989.0 4 2070.0 5 2361.0 6 2984.0 7 3586.0 8 4650.0 9 5895.0 10 7660.0 11 9471.0 12 12180.0 13 14636.0 14 16633.0 15 19757.0 16 23310.0 17 27248.0 18 25343.0 19 22736.0 20 23852.0 21 25735.0 22 28306.0 23 31588.0 24 35371.0 25 39982.0 26 45302.0 27 51364.0 28 59505.0 29 67782.0 30 77991.0 31 91517.0 32 107935.0 33 129539.0 34 157911.0 35 192794.0 36 224455.0 37 213419.0 38 93143.0 39 4405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98385792461274 18.728209637614597 14.526297607738147 22.76163483003452 2 15.573229802762736 20.30933425546797 39.98281476358511 24.134621178184183 3 18.969055959170948 25.109971730911816 29.5758064811928 26.345165828724433 4 12.7900871519059 16.439023009947643 36.4828085634398 34.28808127470667 5 13.61932220271014 37.862972767541216 33.55180457631117 14.965900453437476 6 32.130801941974426 35.96461402813003 17.20450612669146 14.70007790320409 7 29.055968053255743 33.12035154820569 20.209676684750484 17.61400371378808 8 27.22985622207052 34.13589799431254 19.490327552340947 19.14391823127599 9 27.53165550932547 16.232727409786772 19.719597204976097 36.51601987591166 10-11 25.283954043694102 26.671990532246916 26.680316708908485 21.3637387151505 12-13 26.452699076001434 24.15553233994978 27.174983701073764 22.21678488297502 14-15 23.22165152196527 25.279523006037813 25.71763477754644 25.78119069445048 16-17 22.101643234826852 28.575972222622152 26.544081325896506 22.77830321665449 18-19 22.288558776831422 26.551376143186435 29.47131022548931 21.688754854492835 20-21 22.97728035058126 25.763826354814274 29.570972097881427 21.687921196723043 22-23 22.79713169737224 25.666638482405823 29.580646993703567 21.955582826518373 24-25 22.356757288889458 26.03850491005284 29.67961797230933 21.925119828748375 26-27 21.777946752927782 26.381175470045054 29.46540836039467 22.375469416632495 28-29 21.99450783372058 26.4376221768731 29.492927683859012 22.074942305547307 30-31 22.026413721748543 26.270531545184127 29.18754748020071 22.51550725286662 32-33 21.69657068005939 26.32495738190448 29.220998029264905 22.757473908771225 34-35 22.496787059837672 26.399684519127337 29.005596329026023 22.09793209200897 36-37 22.722507413728188 25.861382789258457 28.94469775082437 22.47141204618898 38-39 22.112965963198068 26.298333198519085 29.00595303071592 22.58274780756693 40-41 22.450929651326103 26.143094286118934 29.211599443227566 22.194376619327397 42-43 22.22400231621642 26.149057217718326 28.868988485147863 22.757951980917394 44-45 22.06542667299304 26.41434624752783 29.031669641859377 22.48855743761975 46-47 22.646377029319247 26.671370741370726 28.286555620143528 22.395696609166507 48-49 22.325310553926396 26.985222435517453 27.970646389225845 22.71882062133031 50-51 22.634893583165926 26.865605237565337 27.916086104937644 22.583415074331093 52-53 22.83657432149144 26.573258929576227 28.034595548650938 22.555571200281396 54-55 22.291962083988288 27.067659530588088 27.961331279348713 22.679047106074908 56-57 22.235811755332918 27.263985535827906 27.712876342896987 22.787326365942192 58-59 22.235893908413136 26.562288970063978 28.02754997450628 23.174267147016607 60-61 21.882562284100672 27.007685407410882 28.180717142403182 22.929035166085264 62-63 22.09541526820168 26.915277623652923 28.468097201255425 22.521209906889975 64-65 21.949292404067453 26.853601942545417 28.14997568677659 23.047129966610537 66-67 21.797315179089257 27.03552158555812 28.0687630728773 23.09840016247532 68-69 22.412713285011197 26.60462170413399 28.169285388071085 22.813379622783728 70-71 22.290714026183718 26.557374131040053 27.903836669560146 23.248075173216087 72-73 22.1406880405297 26.945475001249932 27.71051127804197 23.2033256801784 74-75 22.1856850385992 27.261706783255242 27.647764214926244 22.904843963219314 76-77 22.249229269589954 26.840735608451677 27.877144865906782 23.032890256051587 78-79 22.291776799593837 27.027955008191274 27.770377426631843 22.909890765583047 80-81 22.49929798382 27.042134317612792 27.36128016346967 23.09728753509754 82-83 22.497880851930237 26.947005726868444 27.548139794944454 23.006973626256865 84-85 22.23992785384017 27.062111437134334 27.6391655666436 23.05879514238189 86-87 22.511445330943587 26.965990119274323 27.33675288658941 23.185811663192673 88-89 22.453482938399173 26.916084071694197 27.24855916551369 23.381873824392937 90-91 22.324903309442767 26.891990875196797 27.390692679790508 23.392413135569925 92-93 22.516239396783142 26.553269017076396 27.480658659201058 23.4498329269394 94-95 22.36055108010748 26.228956237771573 27.756205620606565 23.654287061514378 96-97 22.79579706001552 25.942373204398773 27.57507830638295 23.686751429202758 98-99 22.459635347818416 26.17350793053317 27.32716138898065 24.039695332667765 100-101 23.009748326458286 25.51972698505316 27.79942588503716 23.671098803451393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1007.0 1 677.0 2 445.0 3 592.0 4 553.0 5 574.0 6 1005.5 7 2060.0 8 3248.0 9 3558.0 10 2940.5 11 2376.0 12 2328.0 13 2351.0 14 2409.0 15 2519.5 16 2621.0 17 2805.5 18 3074.5 19 3337.5 20 3642.0 21 4116.5 22 4753.0 23 5650.5 24 6657.0 25 7919.5 26 9808.0 27 12063.0 28 13990.5 29 16278.0 30 19121.5 31 23711.5 32 30510.5 33 34101.5 34 35044.0 35 38034.0 36 43976.0 37 49380.5 38 52963.5 39 55804.0 40 57106.5 41 58274.0 42 59115.0 43 60713.0 44 62491.5 45 65033.5 46 65873.5 47 65597.5 48 66652.5 49 66220.5 50 64798.0 51 63733.5 52 63106.0 53 63941.0 54 65401.5 55 62605.5 56 57966.5 57 53852.0 58 51153.0 59 46794.5 60 38786.0 61 32526.0 62 27560.0 63 21200.5 64 15150.5 65 11219.5 66 8380.5 67 6425.5 68 5205.0 69 4206.5 70 3350.5 71 2363.0 72 1673.5 73 1305.0 74 962.5 75 752.5 76 554.5 77 351.5 78 258.5 79 182.0 80 104.0 81 68.5 82 48.0 83 26.5 84 20.5 85 17.5 86 10.0 87 5.5 88 3.5 89 1.5 90 0.5 91 2.0 92 3.5 93 1.5 94 1.0 95 2.0 96 1.0 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006856248030791357 2 0.10734476878742805 3 0.11624218989608859 4 0.11901609177114157 5 0.15785071802188347 6 0.098761374306132 7 0.025488494587751077 8 0.026064210071252644 9 0.021667837288149784 10-11 0.05369855327932773 12-13 0.053960242135464805 14-15 0.028105383149121824 16-17 0.0325802625890658 18-19 0.03708131091462349 20-21 0.06026694356836831 22-23 0.03226623596170131 24-25 0.05032276703515946 26-27 0.05835661491856766 28-29 0.0542742687628293 30-31 0.06895501359211918 32-33 0.05089848251866103 34-35 0.06280532547289792 36-37 0.054064917677919634 38-39 0.03299896475888513 40-41 0.038180404110399205 42-43 0.03425507126834308 44-45 0.007615145713588874 46-47 0.013895678260878667 48-49 0.008818914451819418 50-51 0.00837404339638639 52-53 0.009891838761981424 54-55 0.012194700695987682 56-57 0.015204122541564042 58-59 0.02051640632114666 60-61 0.025514663473364785 62-63 0.012456389552124757 64-65 0.009577812134616935 66-67 0.011618985212486117 68-69 0.01169749186932724 70-71 0.032658769245906924 72-73 0.029335320772966078 74-75 0.03074844059610628 76-77 0.02433706362074795 78-79 0.005024426037831835 80-81 0.005102932694672957 82-83 0.005783323720629351 84-85 0.005233777122741494 86-87 0.014654575943676184 88-89 0.039174821763720086 90-91 0.037002804257782365 92-93 0.030015711798922467 94-95 0.04843860727097253 96-97 0.045010483255576855 98-99 0.027058627724573528 100-101 0.07675334150500401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1910666.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.039493348516295 #Duplication Level Percentage of deduplicated Percentage of total 1 73.71106768454204 33.93620210374508 2 13.350594033349125 12.293091703942368 3 5.027817493386899 6.9443451013302 4 2.4246461326392947 4.465179179846103 5 1.3369491492220489 3.077623073145652 6 0.8577500203982167 2.369422581528805 7 0.5943220663533154 1.9153600774524948 8 0.4073332043820528 1.500273148302188 9 0.30701913575809103 1.2721504912741668 >10 1.7232680904559712 14.948358682406166 >50 0.1587793378528406 5.108898413909342 >100 0.09234044821138365 7.626997684307689 >500 0.005484982613298577 1.7648398283107176 >1k 0.0022854094222077405 1.641996538526376 >5k 3.4281141333116107E-4 1.1352613919727232 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9569 0.5008201328751336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6720 0.3517098226482284 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5290 0.276866809793025 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2329 0.12189466918864941 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2300 0.12037687382305438 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2125 0.11121776385825674 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.233777122741494E-5 0.0 0.0 0.0 0.0 8 5.233777122741494E-5 0.0 0.0 0.0 0.0 9 5.233777122741494E-5 0.0 0.0 0.0 0.0 10-11 5.233777122741494E-5 0.0 0.0 5.233777122741494E-5 0.0 12-13 5.233777122741494E-5 0.0 0.0 1.0467554245482988E-4 0.0 14-15 5.233777122741494E-5 0.0 0.0 1.0467554245482988E-4 0.0 16-17 5.233777122741494E-5 0.0 0.0 1.0467554245482988E-4 0.0 18-19 5.233777122741494E-5 0.0 0.0 1.0467554245482988E-4 0.0 20-21 5.233777122741494E-5 0.0 0.0 1.831821992959523E-4 0.0 22-23 5.233777122741494E-5 0.0 0.0 7.588976827975167E-4 0.0 24-25 5.233777122741494E-5 0.0 0.0 0.0013084442806853736 0.0 26-27 5.233777122741494E-5 0.0 0.0 0.0026953952182118697 0.0 28-29 7.850665684112241E-5 0.0 0.0 0.006725403602722821 0.0 30-31 1.0467554245482988E-4 0.0 0.0 0.019836015295190263 0.0 32-33 1.0467554245482988E-4 0.0 0.0 0.046632954163626714 0.0 34-35 1.0467554245482988E-4 0.0 0.0 0.0834002384508857 0.0 36-37 1.0467554245482988E-4 0.0 0.0 0.14301295987891133 0.0 38-39 1.5701331368224482E-4 0.0 0.0 0.23763965025807754 0.0 40-41 1.5701331368224482E-4 0.0 0.0 0.3440161702777984 0.0 42-43 1.5701331368224482E-4 0.0 0.0 0.45403016539782465 0.0 44-45 1.5701331368224482E-4 0.0 0.0 0.5906317483013777 0.0 46-47 1.5701331368224482E-4 0.0 0.0 0.7500787683456973 0.0 48-49 1.5701331368224482E-4 0.0 0.0 0.9227410756249392 0.0 50-51 1.5701331368224482E-4 0.0 0.0 1.1207610330638635 0.0 52-53 1.5701331368224482E-4 0.0 0.0 1.3130761734389997 0.0 54-55 1.5701331368224482E-4 0.0 0.0 1.5092643088849647 0.0 56-57 1.5701331368224482E-4 0.0 0.0 1.7415393375922323 0.0 58-59 1.5701331368224482E-4 0.0 0.0 1.986506275822148 0.0 60-61 1.831821992959523E-4 0.0 0.0 2.262378667961852 0.0 62-63 2.0935108490965977E-4 0.0 0.0 2.533985531746522 0.0 64-65 2.0935108490965977E-4 0.0 0.0 2.8134953989865314 0.0 66-67 2.0935108490965977E-4 0.0 0.0 3.136105420832317 0.0 68-69 2.0935108490965977E-4 0.0 0.0 3.4912171986103275 0.0 70-71 2.878577417507822E-4 0.0 0.0 3.865379925115117 0.0 72-73 3.1402662736448964E-4 0.0 0.0 4.254275734220424 2.616888561370747E-5 74-75 3.1402662736448964E-4 0.0 0.0 4.6387228327714 5.233777122741494E-5 76-77 3.1402662736448964E-4 0.0 0.0 5.058262406930359 5.233777122741494E-5 78-79 3.1402662736448964E-4 0.0 0.0 5.521791877805959 5.233777122741494E-5 80-81 3.1402662736448964E-4 0.0 0.0 6.008742501305827 5.233777122741494E-5 82-83 3.1402662736448964E-4 0.0 0.0 6.528770596221422 5.233777122741494E-5 84-85 3.1402662736448964E-4 0.0 0.0 7.038488150205216 5.233777122741494E-5 86-87 3.1402662736448964E-4 0.0 0.0 7.556710592013466 5.233777122741494E-5 88-89 3.1402662736448964E-4 0.0 0.0 8.108612389606556 5.233777122741494E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4070 0.0 60.653687 1 GTATCAA 8225 0.0 46.841778 1 ATCAACG 10965 0.0 34.745354 3 TCAACGC 11110 0.0 34.29188 4 TATCAAC 11230 0.0 34.094868 2 CAACGCA 11300 0.0 33.673203 5 AACGCAG 11785 0.0 32.377934 6 AGGTAAG 760 0.0 28.162539 2 ACGCAGA 13470 0.0 28.119703 7 CGCAGAG 13555 0.0 27.768068 8 TGGTATC 1755 0.0 27.37267 2 GTACATG 10545 0.0 26.297 1 TACATGG 10475 0.0 26.017971 2 GGTAAGG 960 0.0 25.763508 3 GCAGAGT 14605 0.0 24.990772 9 ACATGGG 10700 0.0 24.404024 3 GTAAGGT 975 0.0 24.391487 4 TAAGGTG 1105 0.0 23.67409 5 GTGGTAT 2310 0.0 22.855736 1 TAGATCG 105 0.0021797868 22.649237 5 >>END_MODULE