FastQCFastQC Report
Thu 26 May 2016
SRR938547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910666
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94790.49610973346466625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72390.3788731259152568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55280.28932319934514983No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24360.1274948107099828No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22970.12021986050937213No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20410.1068213910751539No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20070.1050419068534218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40350.060.5502551
GTATCAA78900.046.2077451
ATCAACG106950.033.8008233
TATCAAC108200.033.4752732
TCAACGC109150.032.988984
CAACGCA111300.032.3517275
AACGCAG116400.031.0004546
TAAGGTG10450.027.7291775
TGGTATC16550.027.5720842
GTAAGGT9500.027.5018864
ACGCAGA136200.026.3194777
CGCAGAG136550.026.1824748
AGGTAAG8450.025.8760222
GTACATG104800.024.8097521
GGTAAGG9500.024.501683
TACATGG105800.024.170912
GCAGAGT149200.023.4852399
ACATGGG108550.022.4059583
AAGGTGT12700.021.309766
AGTACTT86050.021.2154812-13
Produced by FastQC (version 0.11.5)