##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938547_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1910666 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80685739946176 31.0 31.0 34.0 30.0 34.0 2 32.02259630934972 31.0 31.0 34.0 30.0 34.0 3 32.074342663762266 33.0 31.0 34.0 30.0 34.0 4 35.76067402675297 37.0 35.0 37.0 35.0 37.0 5 35.660065129122515 37.0 35.0 37.0 33.0 37.0 6 35.66620434968749 37.0 35.0 37.0 33.0 37.0 7 35.599761025736576 37.0 35.0 37.0 33.0 37.0 8 35.57770536556363 37.0 35.0 37.0 33.0 37.0 9 37.15475232196522 39.0 37.0 39.0 33.0 39.0 10-11 37.05697463606931 39.0 37.0 39.0 33.0 39.0 12-13 36.95164251627443 39.0 37.0 39.0 33.0 39.0 14-15 37.9713861554034 40.0 37.0 41.0 33.0 41.0 16-17 37.90310472892698 40.0 37.0 41.0 33.0 41.0 18-19 38.159772822670206 40.0 37.5 41.0 33.0 41.0 20-21 38.13788071803235 40.0 37.0 41.0 33.0 41.0 22-23 38.0254094645532 40.0 37.0 41.0 33.0 41.0 24-25 37.89556913662566 40.0 37.0 41.0 32.5 41.0 26-27 37.7011570834463 40.0 37.0 41.0 32.0 41.0 28-29 37.58866201628123 39.5 37.0 41.0 32.0 41.0 30-31 37.31312223067768 39.0 36.0 41.0 31.5 41.0 32-33 37.0843540943315 39.0 36.0 41.0 31.0 41.0 34-35 36.972694337995236 39.0 35.5 41.0 31.0 41.0 36-37 36.909406981649326 39.0 35.0 41.0 31.0 41.0 38-39 36.73923019512568 39.0 35.0 41.0 30.0 41.0 40-41 36.556029939298654 39.0 35.0 41.0 30.0 41.0 42-43 36.31592046961635 39.0 35.0 40.0 30.0 41.0 44-45 35.97957832504477 38.5 35.0 40.0 29.5 41.0 46-47 35.54781421766023 38.0 34.5 40.0 27.5 41.0 48-49 35.15332480925499 38.0 34.0 40.0 26.5 41.0 50-51 34.76589602787719 38.0 34.0 40.0 25.5 41.0 52-53 34.277815955274235 38.0 33.0 40.0 24.0 41.0 54-55 33.94214739781835 37.0 33.0 40.0 23.0 41.0 56-57 33.608003439638324 37.0 33.0 40.0 23.0 41.0 58-59 33.13586100344068 36.0 32.0 39.0 22.0 41.0 60-61 32.73565055326258 36.0 31.0 39.0 20.5 40.0 62-63 32.265893410988625 35.0 31.0 39.0 18.5 40.0 64-65 31.7359051765196 35.0 30.5 38.0 17.5 40.0 66-67 31.234326407650528 35.0 30.0 38.0 13.5 40.0 68-69 30.760478283488585 34.0 29.0 37.0 10.0 39.5 70-71 30.207940058597366 34.0 29.0 36.5 8.0 39.0 72-73 29.732834781170546 34.0 28.5 36.0 7.0 39.0 74-75 29.163203825263025 34.0 28.0 35.5 4.0 38.5 76-77 27.94090908615111 31.5 26.0 34.5 2.0 36.0 78-79 28.643278574067892 33.0 28.0 35.0 2.0 37.0 80-81 28.295620479979235 33.0 27.0 35.0 2.0 36.5 82-83 27.878366496289775 33.0 26.0 35.0 2.0 36.0 84-85 27.351016347179467 33.0 26.0 35.0 2.0 36.0 86-87 26.783066218794914 32.0 25.0 35.0 2.0 35.0 88-89 26.0589464616003 32.0 23.5 34.0 2.0 35.0 90-91 25.473812011099795 31.0 21.5 34.0 2.0 35.0 92-93 24.675959325177715 31.0 18.5 34.0 2.0 35.0 94-95 23.898912473451666 31.0 11.5 34.0 2.0 35.0 96-97 22.860227794915488 30.0 2.0 34.0 2.0 35.0 98-99 21.53605261202115 29.5 2.0 34.0 2.0 35.0 100-101 17.60868644755284 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21465013634225372 1101 2 0.26123060880952664 1101 3 0.23824930282061985 1101 4 0.23950074647191144 1101 5 0.10561212042578205 1101 6 0.1765802443420057 1101 7 0.27646422461069875 1101 8 0.26825678667555763 1101 9 0.24404274979596607 1101 10-11 0.21487136908159243 1101 12-13 0.28342302083302684 1101 14-15 0.3161185779135707 1101 16-17 0.4719714318355557 1101 18-19 0.4907216024630401 1101 20-21 0.3707133862001868 1101 22-23 0.43388574788570367 1101 24-25 0.43401436566710316 1101 26-27 0.35682080297760166 1101 28-29 0.2763203919817343 1101 30-31 0.30728935940434354 1101 32-33 0.2733016324742863 1101 34-35 0.3895554037708564 1101 36-37 0.4491258751256382 1101 38-39 0.4101551935456129 1101 40-41 0.52569729999054 1101 42-43 0.5715808622760434 1101 44-45 0.6522714514709662 1101 46-47 0.5159864454578198 1101 48-49 0.41484965864691503 1101 50-51 0.34264367804073714 1101 52-53 0.3279506155371763 1101 54-55 0.4488939085572028 1101 56-57 0.6310631129550544 1101 58-59 0.8381662725816597 1101 60-61 0.7037324558599707 1101 62-63 0.49403574008614015 1101 64-65 0.34812779525742954 1101 66-67 0.3248068444702561 1101 68-69 0.2207574319740715 1101 70-71 0.3105164456849785 1101 72-73 0.31416522071084074 1101 74-75 0.19318827094257074 1101 76-77 0.43852929363327675 1101 78-79 0.5210272337085478 1101 80-81 0.5525142447053355 1101 82-83 0.5118477333983051 1101 84-85 0.5224124924153344 1101 86-87 0.5800378512553728 1101 88-89 0.5980959833371671 1101 90-91 0.7675249650405185 1101 92-93 0.8754288660912017 1101 94-95 0.937003641957272 1101 96-97 0.8994907243032948 1101 98-99 1.0027321094761312 1101 100-101 1.2259609758384578 1102 1 0.3006749810627518 1102 2 0.21917056740165464 1102 3 0.30673273967362036 1102 4 0.30013739243464954 1102 5 0.12310694444234116 1102 6 0.2602448406153002 1102 7 0.17358120183637027 1102 8 0.09554974733808308 1102 9 0.06511935435083416 1102 10-11 0.2826560481706224 1102 12-13 0.31335573304834696 1102 14-15 0.3350963211847926 1102 16-17 0.4701779535746837 1102 18-19 0.3378903395230779 1102 20-21 0.15584537377782226 1102 22-23 0.14752964022524395 1102 24-25 0.2849952145283936 1102 26-27 0.2528741156070069 1102 28-29 0.2593949261432229 1102 30-31 0.25857041530495906 1102 32-33 0.36087927222584426 1102 34-35 0.28199329196961287 1102 36-37 0.3986781525583467 1102 38-39 0.21593159106114257 1102 40-41 0.05103632690565263 1102 42-43 -0.047257129442385803 1102 44-45 -0.02050028765946621 1102 46-47 0.09361066906030402 1102 48-49 0.002748209371546295 1102 50-51 0.03288436127055405 1102 52-53 0.1729583795123233 1102 54-55 0.3112282770872312 1102 56-57 0.43338712951820213 1102 58-59 0.4510518833477022 1102 60-61 0.38297935026991325 1102 62-63 0.5383499222807586 1102 64-65 0.39390936875639326 1102 66-67 0.5293487699360959 1102 68-69 0.5700120903591639 1102 70-71 0.6151955347118907 1102 72-73 0.4690280571497638 1102 74-75 0.6695029707355289 1102 76-77 0.5735758774842061 1102 78-79 0.777882823770657 1102 80-81 0.7359510977074066 1102 82-83 0.6687447313279122 1102 84-85 0.7833389934505313 1102 86-87 0.7338525510483329 1102 88-89 0.6331839750555908 1102 90-91 0.7956874909204323 1102 92-93 0.952020480998037 1102 94-95 0.9452593355390526 1102 96-97 1.0319337884854853 1102 98-99 0.9797533310082258 1102 100-101 1.1620660483022256 1103 1 0.2821507406448909 1103 2 0.278145039556442 1103 3 0.3388002253893063 1103 4 0.15217397989388104 1103 5 0.06897123733819654 1103 6 0.13375130227449006 1103 7 0.21180283552037338 1103 8 0.18115361207238578 1103 9 0.24318061137037716 1103 10-11 0.28141717522131415 1103 12-13 0.37872859653199953 1103 14-15 0.4921040947937598 1103 16-17 0.5645299484740178 1103 18-19 0.48915545146795125 1103 20-21 0.491748657330632 1103 22-23 0.33326935919824763 1103 24-25 0.4528036453383706 1103 26-27 0.34253055642213326 1103 28-29 0.3521583583019421 1103 30-31 0.3955673990465982 1103 32-33 0.32830122960587005 1103 34-35 0.39166542713722663 1103 36-37 0.43859036227413384 1103 38-39 0.5950919432031725 1103 40-41 0.6441783853180709 1103 42-43 0.7020241182586346 1103 44-45 0.6804546155995581 1103 46-47 0.6172444021091792 1103 48-49 0.5876781978460102 1103 50-51 0.8276453902315382 1103 52-53 0.7319848392097228 1103 54-55 0.7177914591171941 1103 56-57 0.9233089029800396 1103 58-59 0.7724418144396878 1103 60-61 0.7663467697750477 1103 62-63 0.7030111449689045 1103 64-65 0.7345601939360229 1103 66-67 0.784418781461639 1103 68-69 0.7539739334646818 1103 70-71 0.6063507861893669 1103 72-73 0.5214707359795554 1103 74-75 0.6912867319851941 1103 76-77 0.7609438855275634 1103 78-79 0.8879139672513681 1103 80-81 0.9006167344321945 1103 82-83 0.9799833590529659 1103 84-85 1.1222261657696144 1103 86-87 1.0258449981411992 1103 88-89 0.9491142332767346 1103 90-91 1.0321984401835351 1103 92-93 1.3254847640849192 1103 94-95 1.1819546934438598 1103 96-97 1.39102645873243 1103 98-99 1.3405478576027967 1103 100-101 1.5248787705367128 1104 1 0.1974241594914794 1104 2 0.19766927889419605 1104 3 0.1689035792130653 1104 4 0.1093106198503051 1104 5 0.053934189873459104 1104 6 0.04136312323581848 1104 7 0.08450916363035077 1104 8 0.18205990172256037 1104 9 0.2495504014700174 1104 10-11 0.1832315026945892 1104 12-13 0.2241914897213988 1104 14-15 0.3645702791487153 1104 16-17 0.3360653687577937 1104 18-19 0.2694202352603625 1104 20-21 0.18103392142896269 1104 22-23 0.2584604390998706 1104 24-25 0.20849049788181873 1104 26-27 0.14410280794925256 1104 28-29 0.28569466371327223 1104 30-31 0.3293981335828562 1104 32-33 0.3411024559031688 1104 34-35 0.31642268412508656 1104 36-37 0.45149371867415056 1104 38-39 0.3438730526305207 1104 40-41 0.4481051497536086 1104 42-43 0.6858544387539425 1104 44-45 0.6618724574524748 1104 46-47 0.4257306775715435 1104 48-49 0.5052656455963458 1104 50-51 0.5044799827094266 1104 52-53 0.5821770002182944 1104 54-55 0.580049233656247 1104 56-57 0.3418288629744808 1104 58-59 0.5811268295613843 1104 60-61 0.36741370731650846 1104 62-63 0.35502628230629085 1104 64-65 0.5887506104520206 1104 66-67 0.5541614044018957 1104 68-69 0.5258727896995445 1104 70-71 0.6387849145733462 1104 72-73 0.6614313008786326 1104 74-75 0.484744240801966 1104 76-77 0.38616873276945185 1104 78-79 0.6501380664592666 1104 80-81 0.5549146175787918 1104 82-83 0.7419295907742054 1104 84-85 0.8426845735153101 1104 86-87 0.8715190522186269 1104 88-89 0.8441507102850387 1104 90-91 0.9883427619798475 1104 92-93 1.193006839368607 1104 94-95 1.2919529544718387 1104 96-97 1.2863352826812928 1104 98-99 1.3911919673181288 1104 100-101 1.7448265277688542 1105 1 0.1884950646481478 1105 2 0.19317874122234002 1105 3 0.22600155985725934 1105 4 0.08028008794828168 1105 5 0.03706016432735737 1105 6 0.15508086392069487 1105 7 0.21349068947173322 1105 8 0.13040716727389423 1105 9 0.1871654263758984 1105 10-11 0.32269576277960255 1105 12-13 0.20056100985763692 1105 14-15 0.2569875370213808 1105 16-17 0.2706315929260086 1105 18-19 0.3577223990642011 1105 20-21 0.2326723612400201 1105 22-23 0.24982662237676578 1105 24-25 0.4444022661804752 1105 26-27 0.2214395408073102 1105 28-29 0.26353494322975024 1105 30-31 0.28910140578423693 1105 32-33 0.3925970829965024 1105 34-35 0.3700939946719046 1105 36-37 0.3111600847202567 1105 38-39 0.12244940491044787 1105 40-41 0.28307139275032256 1105 42-43 0.3408663552836515 1105 44-45 0.2817357371852509 1105 46-47 0.1256585113101849 1105 48-49 0.35059492329552455 1105 50-51 0.3797774627813979 1105 52-53 0.4414939417896022 1105 54-55 0.33692055044072333 1105 56-57 0.26085378387026026 1105 58-59 0.440411138929683 1105 60-61 0.3594270478231536 1105 62-63 0.3671604081689921 1105 64-65 0.3651639963549691 1105 66-67 0.3859913461519682 1105 68-69 0.321970035974779 1105 70-71 0.42379313182411593 1105 72-73 0.28725890985936076 1105 74-75 0.4136874743414012 1105 76-77 0.4807956239572242 1105 78-79 0.6385159485253276 1105 80-81 0.5404236091946295 1105 82-83 0.8173166216971168 1105 84-85 0.699251313445604 1105 86-87 0.6380724591505746 1105 88-89 0.6946197043497335 1105 90-91 0.839464688885446 1105 92-93 0.941145143308411 1105 94-95 1.028553031229709 1105 96-97 1.249827067018817 1105 98-99 1.2963227892424634 1105 100-101 1.6326712342912835 1106 1 0.036154534279774 1106 2 0.07999766977403766 1106 3 0.007149060175791533 1106 4 0.050635869310859505 1106 5 0.0674713864251757 1106 6 -0.013622970701973713 1106 7 0.024087063555192856 1106 8 0.12312105722454447 1106 9 -0.03117714707997976 1106 10-11 0.18341853317016898 1106 12-13 0.08661152553426632 1106 14-15 0.12335811385533901 1106 16-17 0.1771067821540271 1106 18-19 0.08965624001231731 1106 20-21 0.1987559248717048 1106 22-23 0.14377352465245963 1106 24-25 0.18598016791157335 1106 26-27 0.11956155214351583 1106 28-29 0.14683146265204527 1106 30-31 0.08442197996849643 1106 32-33 -0.14429011760153543 1106 34-35 -0.05924274606232416 1106 36-37 0.08916416751265643 1106 38-39 0.027762108923226947 1106 40-41 0.03393395452496151 1106 42-43 0.10085558171795839 1106 44-45 0.07112760858349532 1106 46-47 -0.4837898663407785 1106 48-49 -0.26673208232973167 1106 50-51 -0.030614207916194403 1106 52-53 -0.1255203429098941 1106 54-55 0.02650613179044825 1106 56-57 -0.03687364773648483 1106 58-59 0.12825764352493962 1106 60-61 0.06026680526853312 1106 62-63 -0.15017680161843572 1106 64-65 -0.11791330271042 1106 66-67 -0.20140448401017963 1106 68-69 -0.40845093927251597 1106 70-71 -0.4136039668112339 1106 72-73 0.057403391775444845 1106 74-75 0.20768629945333572 1106 76-77 0.1533192542034918 1106 78-79 0.3180074429897104 1106 80-81 -0.20871566126979246 1106 82-83 0.09718099090512666 1106 84-85 0.3762227744899498 1106 86-87 0.5759849243521238 1106 88-89 0.7323961046595784 1106 90-91 0.7535463481160392 1106 92-93 0.8747104099189258 1106 94-95 0.9165646064704696 1106 96-97 1.1897621832053247 1106 98-99 1.1788145328913586 1106 100-101 1.0619349673458878 1107 1 0.06711002183034509 1107 2 0.11639837111484042 1107 3 0.08701140786055106 1107 4 0.09648014139663275 1107 5 -0.00416969432882297 1107 6 0.003350348722207741 1107 7 0.19051814652582522 1107 8 0.08274248446778643 1107 9 4.6460601990361283E-4 1107 10-11 0.049333328036382795 1107 12-13 0.12463110970934821 1107 14-15 0.1913858607297172 1107 16-17 0.18666197282186658 1107 18-19 0.15606142716085003 1107 20-21 0.1975401869353277 1107 22-23 0.2030888769354391 1107 24-25 0.19196378368270928 1107 26-27 0.16059736926963097 1107 28-29 0.052762393819584474 1107 30-31 0.1510443663551655 1107 32-33 0.2051269900294841 1107 34-35 0.4019505523300353 1107 36-37 0.3338406850971296 1107 38-39 0.3201537798183196 1107 40-41 0.26687107652737296 1107 42-43 0.46635817077490316 1107 44-45 0.4740026488979794 1107 46-47 0.4085864666637349 1107 48-49 0.4091882698954734 1107 50-51 0.45446759241598755 1107 52-53 0.5628804298676613 1107 54-55 0.45009940560016304 1107 56-57 0.4554354769427107 1107 58-59 0.4535672115322029 1107 60-61 0.3508818809442076 1107 62-63 0.6014326263517873 1107 64-65 0.3932524977856424 1107 66-67 0.4289321301609803 1107 68-69 0.7043994290994924 1107 70-71 0.6912119995126424 1107 72-73 0.873554873405169 1107 74-75 0.5791216843871183 1107 76-77 0.7684075363702512 1107 78-79 1.0429457500017527 1107 80-81 0.9557045559609598 1107 82-83 1.092921312013324 1107 84-85 1.1639539853113554 1107 86-87 1.4039000953118261 1107 88-89 1.273571643665619 1107 90-91 1.4158717876884275 1107 92-93 1.739265280171928 1107 94-95 1.8647259505739413 1107 96-97 1.8456265363718138 1107 98-99 1.8090854882843637 1107 100-101 1.9504032898744121 1108 1 0.19908698412922732 1108 2 0.15046834420595445 1108 3 0.20159493074432788 1108 4 0.1907736863783569 1108 5 0.12223080820315602 1108 6 0.11252242978041238 1108 7 0.18411235943137427 1108 8 0.10413150180090724 1108 9 0.12749003299136064 1108 10-11 0.1790551282275743 1108 12-13 0.2509668015695041 1108 14-15 0.28101827204815066 1108 16-17 0.28777222948458814 1108 18-19 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2316 70-71 -0.6235957945894732 2316 72-73 -0.7306323120312967 2316 74-75 -0.7491639848668683 2316 76-77 -0.9416401348086971 2316 78-79 -0.9981038589188067 2316 80-81 -0.9836348048555834 2316 82-83 -0.9039390219472416 2316 84-85 -1.0972093277671995 2316 86-87 -1.245128556494862 2316 88-89 -1.2984391214161093 2316 90-91 -1.2533898190879036 2316 92-93 -1.4679279045735605 2316 94-95 -1.3330356809762485 2316 96-97 -1.6987756144025532 2316 98-99 -1.6032783928217498 2316 100-101 -1.849453425309406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 12.0 4 28.0 5 55.0 6 144.0 7 421.0 8 967.0 9 1599.0 10 2782.0 11 4338.0 12 6191.0 13 8462.0 14 10902.0 15 14436.0 16 18243.0 17 23396.0 18 21342.0 19 17429.0 20 16717.0 21 18016.0 22 19450.0 23 21666.0 24 25121.0 25 29483.0 26 35224.0 27 42047.0 28 50045.0 29 60274.0 30 71720.0 31 84926.0 32 102847.0 33 126315.0 34 160560.0 35 211532.0 36 272273.0 37 294339.0 38 132555.0 39 4805.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69002817868552 19.539332923004775 15.116891300615912 22.653747597693787 2 15.954004040539452 21.000279632979097 39.06807858629296 23.977637740188495 3 18.900634647813906 25.332946731663203 30.029947672696327 25.736470947826568 4 13.108263353335095 17.101532712432583 36.18771474852996 33.60248918570236 5 13.517967160305838 38.0895106301204 33.63340562855476 14.759116581018997 6 32.05876903655584 36.39427299172121 16.91781818486329 14.62913978685966 7 28.899608827497847 33.272063249149774 20.134759293356346 17.693568629996033 8 27.11038978031744 34.61866176505993 19.298663397998396 18.972285056624237 9 27.201248151168233 16.281495562280377 19.728827539716516 36.788428746834875 10-11 25.207676276230384 26.82117125651474 26.644845305249582 21.32630716200529 12-13 26.28177818624501 24.20273873089279 27.312858448310696 22.202624634551512 14-15 23.00585240957865 25.383452680897655 25.736863481110777 25.87383142841292 16-17 21.919895994381015 28.743982464753127 26.62710280080349 22.709018740062366 18-19 22.129796625888567 26.6492678469183 29.56374897548813 21.657186551705006 20-21 22.80958576747584 25.83374069565272 29.710163890496823 21.646509646374614 22-23 22.66217643481383 25.69182682896958 29.740519797808723 21.905476938407865 24-25 22.29542098184739 26.14881095851119 29.78472446306734 21.771043596574078 26-27 21.74527113156043 26.42924603723952 29.554626310725013 22.270856520475036 28-29 21.845750522920167 26.493793127992905 29.64950152159105 22.01095482749588 30-31 21.91712279798224 26.277821916704085 29.311239096551194 22.493816188762477 32-33 21.651037678622924 26.34428749452414 29.3374170901244 22.667257736728534 34-35 22.46115643343995 26.45301385562523 29.110893419696865 21.974936291237963 36-37 22.625698470249382 25.864967129887983 29.12771444096729 22.381619958895346 38-39 22.066450136750916 26.26593715398459 29.163418325381603 22.50419438388289 40-41 22.321827650276084 26.155837018815586 29.366838510454556 22.15549682045377 42-43 22.218334636997383 26.14932008402418 28.984521159265704 22.647824119712734 44-45 22.001943461649084 26.39396788865373 29.16532431025562 22.438764339441565 46-47 22.611016263439033 26.74266459967362 28.356473606585347 22.289845530302 48-49 22.293561512059146 26.969339486859557 28.00010572229788 22.73699327878342 50-51 22.56000033496349 26.88176207543378 27.962568876867422 22.59566871273531 52-53 22.834079844410272 26.631734693557114 28.003455339656437 22.530730122376177 54-55 22.28882020898811 27.047447576329336 27.963922988953932 22.699809225728618 56-57 22.20479665205745 27.186174873054735 27.77241024857301 22.836618226314805 58-59 22.183003846629017 26.59147208419448 28.007652425554014 23.21787164362249 60-61 21.849711043165055 27.08238907271077 28.107214971114786 22.96068491300939 62-63 22.046735094084426 27.015430322511246 28.34812402986352 22.589710553540804 64-65 21.915284872792256 26.901808489310238 28.057128978071887 23.125777659825616 66-67 21.69996341361924 27.10514318674181 27.926216942059 23.26867645757995 68-69 22.29085377535776 26.709440409723396 28.015318562659854 22.984387252258994 70-71 22.166462069328272 26.618423390617973 27.74920858644131 23.46590595361245 72-73 21.975647222302182 26.959409420280373 27.55613883124462 23.508804526172824 74-75 21.95701371086415 27.34074358312607 27.397647517035846 23.304595188973934 76-77 21.972752514442583 26.907409080138816 27.862236168438525 23.257602236980077 78-79 22.01482848090265 27.06449059424912 27.693067617119034 23.227613307729197 80-81 22.120146059915978 27.07793788527229 27.34966691533498 23.45224913947675 82-83 22.17212030080699 26.967864583974148 27.476190388930238 23.383824726288623 84-85 21.83992099982438 27.032565063779344 27.534168020894324 23.593345915501953 86-87 21.972625196972803 26.97916936559644 27.313247799832453 23.73495763759831 88-89 21.931603923687508 26.980471920512144 27.070699028297263 24.01722512750308 90-91 21.748814131272958 26.891775376753152 27.371907593067334 23.987502898906556 92-93 21.80022426830111 26.642723425462723 27.376778074894304 24.180274231341862 94-95 21.563634555130054 26.259642491617218 27.716113529459495 24.460609423793233 96-97 21.862738527686123 25.96015803588164 27.576015238127148 24.60108819830508 98-99 21.497366667495786 26.261008220081926 27.377232245133605 24.864392867288682 100-101 21.44982544190457 25.945517564294935 27.81295810213121 24.791698891669288 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1062.0 1 705.5 2 467.0 3 601.0 4 549.5 5 551.0 6 979.0 7 2105.5 8 3296.0 9 3556.0 10 2925.0 11 2329.0 12 2190.5 13 2250.0 14 2392.0 15 2536.0 16 2700.0 17 2917.0 18 3129.5 19 3281.5 20 3583.0 21 4058.5 22 4743.5 23 5572.0 24 6469.5 25 7734.5 26 9577.5 27 11842.5 28 13871.5 29 16234.0 30 19291.0 31 23731.5 32 30911.5 33 34936.0 34 35289.5 35 37876.5 36 43808.5 37 49353.5 38 52820.0 39 55867.0 40 57434.0 41 58182.0 42 58907.0 43 60970.5 44 63159.5 45 65901.0 46 66346.0 47 65995.5 48 67130.0 49 66377.5 50 65200.0 51 64376.5 52 63774.0 53 64631.0 54 66385.5 55 63659.0 56 58706.5 57 54261.0 58 51327.5 59 46883.5 60 38457.0 61 31744.0 62 26908.5 63 20150.0 64 13791.0 65 10177.5 66 7516.5 67 5921.0 68 4733.5 69 3767.5 70 2938.0 71 1981.5 72 1432.5 73 1179.5 74 894.0 75 660.5 76 463.0 77 279.0 78 193.0 79 136.5 80 75.5 81 39.0 82 24.0 83 13.5 84 5.5 85 2.5 86 1.5 87 1.5 88 1.5 89 3.0 90 2.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0467554245482987E-4 2 0.05338452665196324 3 0.0 4 5.2337771227414936E-5 5 0.047313345189583106 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00599267480553901 26-27 0.0018056531073458158 28-29 7.850665684112242E-5 30-31 0.001674808679277278 32-33 3.663643985919046E-4 34-35 0.011226451928280505 36-37 0.021981863915514276 38-39 0.0039515017276698285 40-41 8.37404339638639E-4 42-43 0.001334613166299081 44-45 0.008243198968317853 46-47 0.0 48-49 0.0 50-51 5.233777122741494E-4 52-53 0.0 54-55 0.0016224709080498632 56-57 0.0 58-59 0.022086539457969106 60-61 0.0 62-63 0.009132941079183908 64-65 0.015020940342268089 66-67 0.006228194776062379 68-69 0.015910682453134144 70-71 1.3084442806853735E-4 72-73 5.2337771227414936E-5 74-75 0.02245290385656101 76-77 0.04964237600920307 78-79 0.02920447634489754 80-81 0.025697845672660737 82-83 0.03509247560798172 84-85 0.015204122541564042 86-87 0.009760994333912887 88-89 0.08345257622211313 90-91 0.02423238807829312 92-93 0.023761348137246386 94-95 0.026221223384934884 96-97 0.03768319528373876 98-99 0.053044331138985046 100-101 0.014157367117015741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1910666.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.232051482017056 #Duplication Level Percentage of deduplicated Percentage of total 1 71.23249855999845 30.79527044938559 2 14.30213149173204 12.366209699062741 3 5.566116662703859 7.219039263507787 4 2.719758260245699 4.703229165023328 5 1.5482858316460835 3.3467786391300547 6 0.9664438879225264 2.5068811150288433 7 0.6342355462572564 1.9193512651263245 8 0.45249075868678595 1.5649683019747271 9 0.3469641836831356 1.3499976106264806 >10 1.9367135256709085 15.767703202559874 >50 0.18022635188478198 5.4090295941692155 >100 0.10383694794242229 7.976276404118207 >500 0.0071480177168547114 2.0604532683989767 >1k 0.0027865153811467516 1.849636409675931 >5k 3.6345852797566323E-4 1.1651756122118921 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9479 0.49610973346466625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7239 0.3788731259152568 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5528 0.28932319934514983 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2436 0.1274948107099828 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2297 0.12021986050937213 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2041 0.1068213910751539 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2007 0.1050419068534218 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.233777122741494E-5 0.0 12-13 5.233777122741494E-5 0.0 0.0 1.0467554245482988E-4 0.0 14-15 1.0467554245482988E-4 0.0 0.0 1.0467554245482988E-4 0.0 16-17 1.0467554245482988E-4 0.0 0.0 1.0467554245482988E-4 0.0 18-19 1.3084442806853735E-4 0.0 0.0 1.0467554245482988E-4 0.0 20-21 1.5701331368224482E-4 0.0 0.0 1.831821992959523E-4 0.0 22-23 2.0935108490965977E-4 0.0 0.0 7.588976827975167E-4 0.0 24-25 2.0935108490965977E-4 0.0 0.0 0.0013084442806853736 0.0 26-27 2.0935108490965977E-4 0.0 0.0 0.0026953952182118697 0.0 28-29 2.616888561370747E-4 0.0 0.0 0.0068300791451776505 0.0 30-31 2.616888561370747E-4 0.0 0.0 0.020123873036941048 0.0 32-33 2.616888561370747E-4 0.0 0.0 0.04736568296081052 0.0 34-35 2.616888561370747E-4 0.0 0.0 0.08486569604525333 0.0 36-37 3.1402662736448964E-4 0.0 0.0 0.14510647072800792 0.0 38-39 3.1402662736448964E-4 0.0 0.0 0.2399163433064701 0.0 40-41 3.1402662736448964E-4 0.0 0.0 0.3469994232377611 0.0 42-43 3.1402662736448964E-4 0.0 0.0 0.45795549823988074 0.0 44-45 3.663643985919046E-4 0.0 0.0 0.5954468232542998 0.0 46-47 3.663643985919046E-4 0.0 0.0 0.7562546253505322 0.0 48-49 3.663643985919046E-4 0.0 0.0 0.9303300524529143 0.0 50-51 3.663643985919046E-4 0.0 0.0 1.1288472187184992 0.0 52-53 3.663643985919046E-4 0.0 0.0 1.321397879064159 0.0 54-55 4.1870216981931954E-4 0.0 0.0 1.518711276591513 0.0 56-57 4.1870216981931954E-4 0.0 0.0 1.7520592296089426 0.0 58-59 4.1870216981931954E-4 0.0 0.0 1.9970261678388583 0.0 60-61 4.1870216981931954E-4 0.0 0.0 2.2735004443476776 0.0 62-63 4.1870216981931954E-4 0.0 0.0 2.5462064013281234 0.0 64-65 4.1870216981931954E-4 0.0 0.0 2.8262919840516343 0.0 66-67 4.1870216981931954E-4 0.0 0.0 3.149791748008286 0.0 68-69 4.1870216981931954E-4 0.0 0.0 3.5073110632627578 0.0 70-71 4.1870216981931954E-4 0.0 0.0 3.882782234048232 0.0 72-73 4.1870216981931954E-4 0.0 0.0 4.27521084271139 0.0 74-75 4.1870216981931954E-4 0.0 0.0 4.666226331551407 0.0 76-77 4.1870216981931954E-4 0.0 0.0 5.093878260250614 0.0 78-79 4.1870216981931954E-4 0.0 0.0 5.567979960914153 0.0 80-81 4.710399410467345E-4 0.0 0.0 6.069166458187878 0.0 82-83 4.710399410467345E-4 0.0 0.0 6.604189324560128 0.0 84-85 4.710399410467345E-4 0.0 0.0 7.129058663314257 0.0 86-87 4.710399410467345E-4 0.0 0.0 7.6631917875756415 0.0 88-89 4.710399410467345E-4 0.0 0.0 8.234798756035854 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4035 0.0 60.550255 1 GTATCAA 7890 0.0 46.207745 1 ATCAACG 10695 0.0 33.800823 3 TATCAAC 10820 0.0 33.475273 2 TCAACGC 10915 0.0 32.98898 4 CAACGCA 11130 0.0 32.351727 5 AACGCAG 11640 0.0 31.000454 6 TAAGGTG 1045 0.0 27.729177 5 TGGTATC 1655 0.0 27.572084 2 GTAAGGT 950 0.0 27.501886 4 ACGCAGA 13620 0.0 26.319477 7 CGCAGAG 13655 0.0 26.182474 8 AGGTAAG 845 0.0 25.876022 2 GTACATG 10480 0.0 24.809752 1 GGTAAGG 950 0.0 24.50168 3 TACATGG 10580 0.0 24.17091 2 GCAGAGT 14920 0.0 23.485239 9 ACATGGG 10855 0.0 22.405958 3 AAGGTGT 1270 0.0 21.30976 6 AGTACTT 8605 0.0 21.21548 12-13 >>END_MODULE