FastQCFastQC Report
Thu 26 May 2016
SRR938546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900814
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98110.516147292686186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67440.3547953666166179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.2748822346636757No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23330.12273689061633596No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23020.12110601037239835No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20070.10558634353492766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.056.224061
GTATCAA79750.044.7734341
ATCAACG105200.034.0174873
TCAACGC106200.033.6971744
CAACGCA107200.033.337625
TATCAAC107750.033.1242182
AACGCAG110500.032.5072026
ACGCAGA126800.028.1388597
GTACATG101450.027.87561
CGCAGAG127800.027.8071548
TACATGG101850.027.2971822
ACATGGG106000.025.3317723
GTATCGA1151.2623692E-424.8395211
GCAGAGT138750.024.7204139
TGGTATC19700.024.3656562
CATGGGA55550.023.3561654
GTGTAAG8200.022.6433451
GAGTACT77550.022.54092812-13
AGTACTT81650.022.1071712-13
TAAGGTA5600.022.0652224
Produced by FastQC (version 0.11.5)