##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938546_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1900814 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.127238646180004 31.0 31.0 34.0 28.0 34.0 2 31.215732838668064 31.0 31.0 34.0 28.0 34.0 3 31.27070139424478 31.0 31.0 34.0 27.0 34.0 4 34.92053036225533 37.0 35.0 37.0 32.0 37.0 5 34.95179223217001 37.0 35.0 37.0 32.0 37.0 6 34.99682820097074 37.0 35.0 37.0 32.0 37.0 7 34.923054017910225 37.0 35.0 37.0 32.0 37.0 8 34.88611142384263 37.0 35.0 37.0 32.0 37.0 9 36.392848537521296 39.0 37.0 39.0 32.0 39.0 10-11 36.246488346571525 39.0 35.0 39.0 32.0 39.0 12-13 36.110171221382004 39.0 35.0 39.0 31.0 39.0 14-15 37.093885303875076 39.0 36.0 41.0 31.0 41.0 16-17 36.97091824870819 39.0 36.0 41.0 31.0 41.0 18-19 36.99916535757839 39.0 36.0 41.0 31.0 41.0 20-21 37.08122651663972 39.0 36.0 41.0 31.0 41.0 22-23 37.005337976256484 39.0 36.0 41.0 31.0 41.0 24-25 36.890328564499214 39.0 36.0 41.0 30.0 41.0 26-27 36.70223993510149 39.0 36.0 41.0 30.0 41.0 28-29 36.52931612456558 39.0 36.0 41.0 30.0 41.0 30-31 36.28674241666991 39.0 35.0 40.0 30.0 41.0 32-33 35.99342386998413 38.0 35.0 40.0 29.0 41.0 34-35 35.746374974090045 38.0 35.0 40.0 27.5 41.0 36-37 35.45142896674793 38.0 34.5 40.0 27.0 41.0 38-39 35.19530106575394 38.0 34.0 40.0 26.0 41.0 40-41 35.12400187498619 38.0 34.0 40.0 25.5 41.0 42-43 34.9269389324784 38.0 34.0 40.0 25.0 41.0 44-45 34.60348803196946 38.0 33.0 40.0 24.0 41.0 46-47 34.19884612592289 38.0 33.0 40.0 23.0 41.0 48-49 33.80759848149266 38.0 33.0 40.0 21.5 41.0 50-51 33.0422040241707 37.0 32.0 39.5 20.0 40.5 52-53 33.09158891927353 37.0 32.0 39.5 20.0 40.0 54-55 33.230761926206355 37.0 32.5 40.0 18.5 41.0 56-57 32.94295207211226 37.0 32.0 40.0 17.5 41.0 58-59 32.62322720687032 36.5 31.5 39.5 14.5 41.0 60-61 32.190937935011 36.0 31.0 39.0 11.5 41.0 62-63 31.693650720165152 35.5 30.5 39.0 8.0 40.0 64-65 31.212591026791678 35.0 30.0 38.5 7.0 40.0 66-67 30.695899230540178 35.0 29.0 38.0 3.5 40.0 68-69 30.16300095643235 34.5 29.0 37.5 2.0 40.0 70-71 29.567644703795324 34.0 28.0 37.0 2.0 39.0 72-73 29.052913383424155 34.0 27.5 36.0 2.0 39.0 74-75 28.48530761031853 34.0 26.0 36.0 2.0 38.5 76-77 27.807408036767406 33.0 26.0 35.0 2.0 37.5 78-79 27.078878575178845 33.0 25.0 35.0 2.0 37.0 80-81 26.47375755860384 32.0 23.5 35.0 2.0 36.5 82-83 25.855489016810694 32.0 21.5 35.0 2.0 36.0 84-85 25.241007799816288 31.0 19.5 34.5 2.0 35.5 86-87 24.614689285748106 31.0 17.5 34.0 2.0 35.0 88-89 23.850359372353108 31.0 9.0 34.0 2.0 35.0 90-91 23.05502800379206 30.0 3.5 34.0 2.0 35.0 92-93 22.242033676098767 29.5 2.0 34.0 2.0 35.0 94-95 21.319757219801623 29.0 2.0 34.0 2.0 35.0 96-97 20.113736536031407 28.0 2.0 34.0 2.0 35.0 98-99 18.674053589672635 25.5 2.0 33.0 2.0 35.0 100-101 15.217754604080147 10.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6723977402182122 1101 2 0.6141940429878794 1101 3 0.6374936895976013 1101 4 0.5954224878540728 1101 5 0.6042221286514575 1101 6 0.6232664179173426 1101 7 0.4693447979394705 1101 8 0.4722446376690499 1101 9 0.6517867547937328 1101 10-11 0.7123371272964079 1101 12-13 0.7587018325510186 1101 14-15 0.9605749919385929 1101 16-17 1.0000807068864432 1101 18-19 0.9564418704178976 1101 20-21 0.8697881905103273 1101 22-23 0.952762763668531 1101 24-25 0.9401998286177786 1101 26-27 0.991563142572538 1101 28-29 0.9177962483793891 1101 30-31 0.8656798439983788 1101 32-33 1.0498160089925719 1101 34-35 1.1683666308550755 1101 36-37 1.2769763640112117 1101 38-39 1.1639967656828887 1101 40-41 1.0927722674221627 1101 42-43 1.2286944704587341 1101 44-45 1.3325253810206164 1101 46-47 1.4444150033362675 1101 48-49 1.405353078601351 1101 50-51 1.394690023813105 1101 52-53 1.3872560485249608 1101 54-55 1.3935199227682347 1101 56-57 1.3368377903637807 1101 58-59 1.4463130732246015 1101 60-61 1.358785089017104 1101 62-63 1.355632466485016 1101 64-65 1.0055383591124922 1101 66-67 1.1395946503404737 1101 68-69 1.325182354881779 1101 70-71 1.4938008325722159 1101 72-73 1.432382483582991 1101 74-75 1.3341689166582071 1101 76-77 1.3482594841991151 1101 78-79 1.3056671752440003 1101 80-81 1.3961892575724804 1101 82-83 1.1599452901775038 1101 84-85 1.520357812131941 1101 86-87 1.6776158413886897 1101 88-89 1.7332738397469534 1101 90-91 1.4801448171337164 1101 92-93 1.5261304062454464 1101 94-95 1.433297453605018 1101 96-97 1.5872627472171246 1101 98-99 1.8126140793790633 1101 100-101 1.5220792788958857 1102 1 0.6116918185463724 1102 2 0.6932587306501361 1102 3 0.638996233214371 1102 4 0.6049590760198598 1102 5 0.6515789332607582 1102 6 0.6392628904504534 1102 7 0.7039929622559598 1102 8 0.6710560835407904 1102 9 0.8123247637057815 1102 10-11 0.7472633675004658 1102 12-13 0.7425180686581925 1102 14-15 0.8230834744643261 1102 16-17 0.92615924708808 1102 18-19 0.9491778794724794 1102 20-21 0.8584473212888426 1102 22-23 0.8143951719896734 1102 24-25 0.8342838439760172 1102 26-27 0.7831626248269075 1102 28-29 0.8034358281867497 1102 30-31 0.8721583346301287 1102 32-33 0.8617686381454988 1102 34-35 0.8849112509590356 1102 36-37 0.8143677587192428 1102 38-39 0.9013960241705874 1102 40-41 0.8037099072982699 1102 42-43 0.9794842712769167 1102 44-45 1.0239999906664536 1102 46-47 1.0836974615257091 1102 48-49 1.0150387626255508 1102 50-51 1.048757967571639 1102 52-53 1.109669891866531 1102 54-55 1.0706326026565804 1102 56-57 1.111164866726348 1102 58-59 1.2795321360095073 1102 60-61 1.2940094858318503 1102 62-63 1.2042303089306792 1102 64-65 1.2164210578493382 1102 66-67 1.441951010856858 1102 68-69 1.2241972058403334 1102 70-71 1.2182006313437093 1102 72-73 1.2171836580946547 1102 74-75 1.217853083394207 1102 76-77 1.2454117778775853 1102 78-79 1.1428279575917841 1102 80-81 1.2723327968280138 1102 82-83 1.3700512651404928 1102 84-85 1.1976461983474387 1102 86-87 1.2701432145387308 1102 88-89 1.5093332295387114 1102 90-91 1.3015506573241602 1102 92-93 1.1873129642416984 1102 94-95 1.1226820180586934 1102 96-97 1.3244659378053 1102 98-99 1.3783635274224757 1102 100-101 1.1930833115404003 1103 1 0.7142277215842725 1103 2 0.6864849568260531 1103 3 0.6121139988577617 1103 4 0.5840320206388938 1103 5 0.5944931112118468 1103 6 0.5703570449539939 1103 7 0.5006920409425675 1103 8 0.5046556572933341 1103 9 0.5640277333458315 1103 10-11 0.6062379033535734 1103 12-13 0.5566113024067718 1103 14-15 0.6889983281175063 1103 16-17 0.7847021482578711 1103 18-19 0.691910304402036 1103 20-21 0.7485166357747843 1103 22-23 0.7383123322933471 1103 24-25 0.8710905554026098 1103 26-27 0.7263502699575 1103 28-29 0.6799111138162317 1103 30-31 0.7732307201607114 1103 32-33 0.9166108796106158 1103 34-35 0.9720600673635147 1103 36-37 0.9861670676438621 1103 38-39 0.913282566661799 1103 40-41 0.8041761975821231 1103 42-43 0.8130449190705704 1103 44-45 0.8166926519899249 1103 46-47 0.847753099696277 1103 48-49 0.9697253331040372 1103 50-51 0.9899984935352464 1103 52-53 1.1544618449346373 1103 54-55 1.28382548061861 1103 56-57 1.1530487057522336 1103 58-59 1.1039519174919903 1103 60-61 1.1882962719305894 1103 62-63 0.9665961991476415 1103 64-65 0.9238412325332774 1103 66-67 1.1590593147000838 1103 68-69 1.120866359169522 1103 70-71 1.2238081046022344 1103 72-73 1.3655700703399383 1103 74-75 1.2189888344730484 1103 76-77 1.1535164581574975 1103 78-79 1.0793040775835934 1103 80-81 1.122063813524786 1103 82-83 1.3289042271760216 1103 84-85 1.2656470563609794 1103 86-87 1.1823625493417218 1103 88-89 0.9990741863157844 1103 90-91 0.9989842704973633 1103 92-93 1.0704790801963853 1103 94-95 1.0186948501570754 1103 96-97 0.9696817732138641 1103 98-99 0.8480563014177029 1103 100-101 0.5109738793371026 1104 1 0.5026798478305814 1104 2 0.6066100657836486 1104 3 0.5395974163273358 1104 4 0.5175312958086806 1104 5 0.424100677413783 1104 6 0.590614068348998 1104 7 0.5105814285892194 1104 8 0.33494097470370576 1104 9 0.5057663003728194 1104 10-11 0.6200227289981228 1104 12-13 0.5948198763790771 1104 14-15 0.7074318558265276 1104 16-17 0.7304121322696489 1104 18-19 0.6084825799960001 1104 20-21 0.7221043821820956 1104 22-23 0.6677334469936511 1104 24-25 0.7030590637439857 1104 26-27 0.664209713492987 1104 28-29 0.6565948874081755 1104 30-31 0.6703276702802725 1104 32-33 0.5749431641542486 1104 34-35 0.5236996752560117 1104 36-37 0.6673569079015422 1104 38-39 0.7549805804871781 1104 40-41 0.6777163988565178 1104 42-43 0.5724348497572223 1104 44-45 0.6671967702374673 1104 46-47 0.8311071757591648 1104 48-49 0.6203576367589534 1104 50-51 0.37750950582706366 1104 52-53 0.4431978435921877 1104 54-55 0.5640811988530956 1104 56-57 0.7690602284550323 1104 58-59 0.6335781288440856 1104 60-61 0.7286504365579347 1104 62-63 0.6906246702919496 1104 64-65 0.8080590451774867 1104 66-67 0.772327203556344 1104 68-69 0.6693157818690594 1104 70-71 0.5708307071855216 1104 72-73 0.4366910633806391 1104 74-75 0.5472739933996458 1104 76-77 0.5638673132706948 1104 78-79 0.38905455886153106 1104 80-81 0.6571828861150166 1104 82-83 0.5391730436285016 1104 84-85 0.4895137808276111 1104 86-87 0.4898476448748106 1104 88-89 0.7495088513789696 1104 90-91 0.6208135461798925 1104 92-93 0.7952295847807562 1104 94-95 0.5732901804792156 1104 96-97 0.7900683804025022 1104 98-99 0.71959727646583 1104 100-101 0.665650782191209 1105 1 0.3744231925647057 1105 2 0.4205855696142251 1105 3 0.4271386166485094 1105 4 0.4270285520780064 1105 5 0.32619052627569545 1105 6 0.38735708037527417 1105 7 0.35754646764912223 1105 8 0.2617692042987372 1105 9 0.45919163851488776 1105 10-11 0.4394785964118242 1105 12-13 0.4659532567351192 1105 14-15 0.6340449343500154 1105 16-17 0.5172728341501127 1105 18-19 0.5864107417186304 1105 20-21 0.48472753953516445 1105 22-23 0.7489319050037011 1105 24-25 0.7760320966643164 1105 26-27 0.6749526279249665 1105 28-29 0.6007159398296267 1105 30-31 0.6790250968960123 1105 32-33 0.6067877407946085 1105 34-35 0.6379896989004479 1105 36-37 0.6614138586119864 1105 38-39 0.6779653690846175 1105 40-41 0.6462613121972112 1105 42-43 0.7075998506666252 1105 44-45 0.6635582772709085 1105 46-47 0.8432913805652404 1105 48-49 0.855927654015403 1105 50-51 0.6675912632342502 1105 52-53 0.5648473647146943 1105 54-55 0.5514400752765383 1105 56-57 0.6994342364311521 1105 58-59 0.7483099885034861 1105 60-61 0.7465465696055915 1105 62-63 0.953497136721726 1105 64-65 0.854470014776858 1105 66-67 0.9464389845192436 1105 68-69 0.7966608176462451 1105 70-71 0.7469804345868916 1105 72-73 0.7506838680190278 1105 74-75 0.6522810246253847 1105 76-77 0.5590683353491883 1105 78-79 0.7012622750218185 1105 80-81 0.7915382979407859 1105 82-83 0.8192859868507973 1105 84-85 0.7258703194239366 1105 86-87 0.958321580542421 1105 88-89 0.7839130603549869 1105 90-91 0.8742087188109018 1105 92-93 0.8341346654513195 1105 94-95 0.8504069786763822 1105 96-97 0.9674216229731378 1105 98-99 1.190952018439809 1105 100-101 1.0253576444013657 1106 1 0.16850581412693444 1106 2 0.25385655389602846 1106 3 0.12141996164224977 1106 4 0.46443684937641905 1106 5 0.4387288638572713 1106 6 0.08682189878933144 1106 7 0.12476628376476384 1106 8 0.2554113014714332 1106 9 0.2845224710844434 1106 10-11 0.03649202016261199 1106 12-13 0.0890593334437213 1106 14-15 0.2921471085748095 1106 16-17 0.20274029049586062 1106 18-19 0.06109645998821378 1106 20-21 0.31551986561390066 1106 22-23 0.41014968967387944 1106 24-25 0.40537506235930465 1106 26-27 0.35221140652972593 1106 28-29 0.24918886985528843 1106 30-31 0.30072917015954204 1106 32-33 0.43490982703922043 1106 34-35 0.43445802189800986 1106 36-37 0.31370524293664204 1106 38-39 0.42868847637996765 1106 40-41 0.663538234375963 1106 42-43 0.18376975976737953 1106 44-45 0.5496270051972161 1106 46-47 0.22139926823039247 1106 48-49 0.6057829758753783 1106 50-51 0.204494533215275 1106 52-53 0.5916162214768192 1106 54-55 0.4222684277708666 1106 56-57 0.08745774882529389 1106 58-59 -0.1599596779460981 1106 60-61 0.5031235443275861 1106 62-63 0.6177440933794962 1106 64-65 0.6967330076106819 1106 66-67 0.11610194821906106 1106 68-69 0.16752020296394932 1106 70-71 0.1531761448587865 1106 72-73 0.6219088455025208 1106 74-75 0.6008380975301328 1106 76-77 0.5169725229948945 1106 78-79 0.6089132688373624 1106 80-81 0.5955851481517236 1106 82-83 0.374790812950792 1106 84-85 0.3037434345696006 1106 86-87 0.7689371867367427 1106 88-89 0.8402230394340435 1106 90-91 0.8767252115520563 1106 92-93 0.9107966502710241 1106 94-95 0.733111975272692 1106 96-97 0.9127124557920929 1106 98-99 0.7476637237940622 1106 100-101 0.1253434174814263 1107 1 0.508355392381727 1107 2 0.44076845125782427 1107 3 0.46746906620855455 1107 4 0.459093959620148 1107 5 0.4090506556651974 1107 6 0.3283891322006127 1107 7 0.41807799900445275 1107 8 0.31724571143428193 1107 9 0.2346088294545794 1107 10-11 0.42515358299260697 1107 12-13 0.3688673998285097 1107 14-15 0.5505240307966943 1107 16-17 0.515340222520706 1107 18-19 0.5078500586098968 1107 20-21 0.5793441519816795 1107 22-23 0.6253383420985443 1107 24-25 0.6185738918186203 1107 26-27 0.6758468801540758 1107 28-29 0.6619666488206093 1107 30-31 0.5994957310870888 1107 32-33 0.39698535641175425 1107 34-35 0.5763997064847501 1107 36-37 0.6503815786056393 1107 38-39 0.6148492803678423 1107 40-41 0.6437332567992158 1107 42-43 0.6585652464737493 1107 44-45 0.686318517708493 1107 46-47 0.67183725907298 1107 48-49 0.7896082027798599 1107 50-51 0.5676926679599816 1107 52-53 0.410839990121886 1107 54-55 0.49137943889056857 1107 56-57 0.45169635229928673 1107 58-59 0.44516106345333384 1107 60-61 0.45225894527410304 1107 62-63 0.61708865686359 1107 64-65 0.5890153695548577 1107 66-67 0.612277544284197 1107 68-69 0.6220586132004051 1107 70-71 0.4437418669985931 1107 72-73 0.4164686422610906 1107 74-75 0.5488884076934859 1107 76-77 0.3645334334344241 1107 78-79 0.45904569213017865 1107 80-81 0.39614380557832973 1107 82-83 0.5614868143872478 1107 84-85 0.45055132781013185 1107 86-87 0.2381974011791108 1107 88-89 0.40495547811746135 1107 90-91 0.4966634140154049 1107 92-93 0.648223918995189 1107 94-95 0.4243228200884097 1107 96-97 0.5462716070004703 1107 98-99 0.5498156824617482 1107 100-101 0.3286121031668845 1108 1 0.3505216281751018 1108 2 0.30847205921190834 1108 3 0.3454729847165936 1108 4 0.320907610986751 1108 5 0.37396293636695077 1108 6 0.3347343511330223 1108 7 0.3133818866196876 1108 8 0.3953130414832202 1108 9 0.4036595261685605 1108 10-11 0.326006996647223 1108 12-13 0.5217735588776691 1108 14-15 0.5469784720434632 1108 16-17 0.3940626019168363 1108 18-19 0.5114830114739775 1108 20-21 0.47051206249010846 1108 22-23 0.5253228956975562 1108 24-25 0.5004353457314394 1108 26-27 0.4585536180367811 1108 28-29 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-0.8693453824049691 2316 68-69 -0.8873234117538864 2316 70-71 -1.3046419907726055 2316 72-73 -1.1273158668919514 2316 74-75 -1.0052386062418144 2316 76-77 -0.9272759186265951 2316 78-79 -1.094736345343609 2316 80-81 -1.2089230448303034 2316 82-83 -0.9620532838907714 2316 84-85 -0.891269370041119 2316 86-87 -1.0455613877809036 2316 88-89 -1.302669261136483 2316 90-91 -1.3791402269476372 2316 92-93 -1.4758748616430495 2316 94-95 -1.471662634286325 2316 96-97 -1.653873080592163 2316 98-99 -1.4001975964854374 2316 100-101 -1.2908880652939363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5627.0 3 1872.0 4 1870.0 5 2088.0 6 2623.0 7 3321.0 8 4268.0 9 5438.0 10 6863.0 11 8847.0 12 11048.0 13 13307.0 14 14934.0 15 18772.0 16 21906.0 17 25954.0 18 23461.0 19 20779.0 20 21344.0 21 23519.0 22 25845.0 23 29005.0 24 32656.0 25 36433.0 26 41802.0 27 47663.0 28 54735.0 29 63214.0 30 73712.0 31 86748.0 32 103970.0 33 125743.0 34 156009.0 35 195032.0 36 234335.0 37 235229.0 38 114166.0 39 6676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.086091779790905 18.75393975936635 14.468588390807263 22.691380070035482 2 15.639264118845903 20.33584367788988 39.99153627191727 24.033355931346954 3 19.02171052493068 25.137375072898266 29.505112879149937 26.335801523021114 4 12.770520045920309 16.388560347781596 36.50627719675568 34.33464240954242 5 13.604963396216284 38.040246001009656 33.4333333157864 14.921457286987666 6 32.13219505081894 35.917807397081575 17.24411674748248 14.705880804617003 7 28.98963674668264 33.20398783419005 20.171740227643166 17.634635191484143 8 27.226539434338203 34.10795802006793 19.53865650323712 19.126846042356746 9 27.526705488046215 16.23866306242579 19.75006105314571 36.48457039638229 10-11 25.291662192916526 26.706656729678123 26.65249803681317 21.349183040592177 12-13 26.437760718469843 24.14764862597485 27.222481866152147 22.192108789403157 14-15 23.20405614566169 25.304966136509677 25.71146485317576 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22.379289005453806 26.164722436805516 29.265456837886596 22.190531719854086 42-43 22.212355875698766 26.13284733025872 28.92896993500577 22.725826859036744 44-45 22.021629120895742 26.377599699483014 29.10619082355176 22.494580356069488 46-47 22.64226491575703 26.745104373731305 28.27882523911197 22.333805471399693 48-49 22.252826753797507 27.024739686097476 27.95889697409752 22.7635365860075 50-51 22.517771635990176 26.927757530222703 27.984348438237415 22.570122395549713 52-53 22.739159887143128 26.62640824460214 28.079841632083973 22.554590236170757 54-55 22.324364410848364 27.052377949599038 28.025166544469606 22.598091095082996 56-57 22.19541844884722 27.252650350973052 27.772579248775877 22.779351951403854 58-59 22.237424843702524 26.539436421488222 28.040286800771852 23.1828519340374 60-61 21.90889216854633 26.987590793457873 28.195416722569917 22.908100315425877 62-63 22.10419736211807 26.897304429693968 28.531755833746992 22.46674237444097 64-65 21.966161548668424 26.841474463323088 28.233767108368696 22.958596879639792 66-67 21.7775731788319 27.029008476972756 28.079179792054397 23.114238552140947 68-69 22.36464395939503 26.611971458245424 28.24156842855143 22.78181615380811 70-71 22.3509047314721 26.582974214764967 27.827494691166805 23.238626362596136 72-73 22.15991233643655 26.92749997040171 27.75259511246693 23.159992580694812 74-75 22.1370018998297 27.2453890125595 27.68984023908229 22.92776884852851 76-77 22.284559753970488 26.810271574278445 27.939187145010614 22.96598152674045 78-79 22.29876385568855 27.04370501841813 27.798028998134118 22.8595021277592 80-81 22.466010074849784 27.068825866199536 27.41672885953321 23.04843519941747 82-83 22.488308890977596 26.989264198676644 27.55871334534472 22.96371356500104 84-85 22.23728387312823 27.08111431196768 27.640274813602204 23.041327001301884 86-87 22.482694904450724 26.93323428046501 27.447342869101053 23.136727945983214 88-89 22.482059394435986 26.92263062047538 27.299664224591936 23.295645760496697 90-91 22.340949579102208 26.884961620542025 27.45598269569313 23.318106104662636 92-93 22.534948163162642 26.579679075724616 27.52448713050927 23.360885630603466 94-95 22.365524171744468 26.282435354269733 27.84899458394413 23.503045890041673 96-97 22.761893609770922 25.978098838104284 27.641750356226098 23.618257195898696 98-99 22.441730259010537 26.177339761501084 27.469761406366917 23.91116857312146 100-101 22.95849488724981 25.623430001437985 27.77893366777474 23.639141443537465 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1090.0 1 735.0 2 487.0 3 660.5 4 600.0 5 550.5 6 1001.5 7 2078.5 8 3276.0 9 3655.0 10 3037.5 11 2429.5 12 2330.0 13 2416.0 14 2511.5 15 2568.0 16 2695.5 17 2872.0 18 3106.0 19 3352.5 20 3676.0 21 4136.5 22 4761.0 23 5751.5 24 6720.5 25 7961.5 26 9808.5 27 11926.0 28 13815.0 29 15994.0 30 18930.0 31 23473.0 32 30211.0 33 33986.0 34 34881.0 35 37980.0 36 43874.0 37 49219.0 38 52575.5 39 55076.0 40 56661.5 41 58137.0 42 58851.0 43 60317.0 44 62259.5 45 64719.0 46 65643.5 47 65826.5 48 66446.0 49 65498.5 50 64807.0 51 63894.0 52 62865.0 53 63574.5 54 65012.5 55 62238.5 56 57529.5 57 53558.5 58 50791.5 59 46495.5 60 38637.5 61 32125.5 62 27184.5 63 20853.5 64 14833.0 65 11006.0 66 8220.5 67 6399.5 68 5048.0 69 4063.5 70 3213.0 71 2218.5 72 1619.5 73 1314.0 74 985.5 75 721.0 76 552.5 77 375.0 78 247.5 79 156.0 80 87.5 81 43.0 82 27.5 83 23.0 84 18.0 85 10.0 86 9.0 87 8.0 88 4.5 89 4.0 90 3.5 91 1.5 92 2.0 93 2.5 94 2.5 95 1.0 96 0.0 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18360555004329726 2 0.049557715799652145 3 0.047663790355079456 4 0.05266164916714628 5 0.0603425690256911 6 0.06455129223585264 7 0.03866764449335916 8 0.03514283880484887 9 0.04366550330542599 10-11 0.04429681178695022 12-13 0.04082461513856695 14-15 0.03603719248700819 16-17 0.03372239472141935 18-19 0.042139841141742435 20-21 0.05060989660219254 22-23 0.0472429180340633 24-25 0.06755000752309274 26-27 0.053056216968098924 28-29 0.04537529710955412 30-31 0.053687525449623165 32-33 0.05032054688149393 34-35 0.04882118923787388 36-37 0.0472429180340633 38-39 0.027356700866050018 40-41 0.02577842966223944 42-43 0.021806447132649485 44-45 0.004708509091368224 46-47 0.012626169630484624 48-49 0.011784424988452315 50-51 0.009101363941974333 52-53 0.0092854955824189 54-55 0.010285067344832267 56-57 0.008917232301529767 58-59 0.010074631184324188 60-61 0.010995289386547027 62-63 0.004445463890733128 64-65 0.00715482945727462 66-67 0.004024591569716974 68-69 0.008417446420323082 70-71 0.023332109296333045 72-73 0.01928121320655256 74-75 0.02001773976833083 76-77 0.012047470189087412 78-79 0.005550253733400533 80-81 0.007391570137846207 82-83 0.006181562214924764 84-85 0.005655471813654571 86-87 0.007654615338481304 88-89 0.03080259299436978 90-91 0.03729980945005666 92-93 0.022516669174364245 94-95 0.03316999980008565 96-97 0.036116106047198726 98-99 0.022516669174364245 100-101 0.1223160182953198 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1900814.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.663966625848 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4025119672309 33.5184985672504 2 13.402747710289098 12.24045248274604 3 5.125089744212275 7.0209578110255535 4 2.4736754551975997 4.518313337172901 5 1.359565649393155 3.1041580219769194 6 0.884742848792731 2.4240520751837367 7 0.5999448711926978 1.917710380284442 8 0.4302645916001583 1.5718070360890994 9 0.3151069844069494 1.2950131337577486 >10 1.7428834697127855 14.992478899538947 >50 0.1612724996591599 5.138039667647741 >100 0.094065896470693 7.685268156449896 >500 0.00545758080813476 1.7585549872463653 >1k 0.002322374811972238 1.659823511707311 >5k 3.4835622179583574E-4 1.154871931922986 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9811 0.516147292686186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6744 0.3547953666166179 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.2748822346636757 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2333 0.12273689061633596 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2302 0.12110601037239835 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2007 0.10558634353492766 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.2609040127019265E-5 0.0 14-15 0.0 0.0 0.0 5.2609040127019265E-5 0.0 16-17 0.0 0.0 0.0 1.0521808025403853E-4 0.0 18-19 0.0 0.0 0.0 1.0521808025403853E-4 0.0 20-21 0.0 0.0 0.0 1.3152260031754816E-4 5.2609040127019265E-5 22-23 5.2609040127019265E-5 0.0 0.0 4.471768410796638E-4 5.2609040127019265E-5 24-25 2.630452006350963E-4 0.0 0.0 0.0013941395633660105 5.2609040127019265E-5 26-27 2.630452006350963E-4 0.0 0.0 0.002761974606668512 5.2609040127019265E-5 28-29 2.630452006350963E-4 0.0 0.0 0.006313084815242312 5.2609040127019265E-5 30-31 2.630452006350963E-4 0.0 0.0 0.019623171967378186 5.2609040127019265E-5 32-33 2.630452006350963E-4 0.0 0.0 0.04521746998917306 5.2609040127019265E-5 34-35 2.630452006350963E-4 0.0 0.0 0.0846216410443105 5.2609040127019265E-5 36-37 2.630452006350963E-4 0.0 0.0 0.14980424176168736 5.2609040127019265E-5 38-39 2.630452006350963E-4 0.0 0.0 0.25160273440746966 5.2609040127019265E-5 40-41 2.630452006350963E-4 0.0 0.0 0.36216063223440065 5.2609040127019265E-5 42-43 2.89349720698606E-4 0.0 0.0 0.4734024475829829 5.2609040127019265E-5 44-45 3.156542407621156E-4 0.0 0.0 0.6112644372358369 5.2609040127019265E-5 46-47 3.945678009526445E-4 0.0 0.0 0.7756413831127086 5.2609040127019265E-5 48-49 4.208723210161541E-4 0.0 0.0 0.9524866714996838 5.2609040127019265E-5 50-51 4.208723210161541E-4 0.0 0.0 1.1519275426212139 5.2609040127019265E-5 52-53 4.208723210161541E-4 0.0 0.0 1.3473175176529635 5.2609040127019265E-5 54-55 4.208723210161541E-4 0.0 0.0 1.5489416639397646 5.2609040127019265E-5 56-57 4.208723210161541E-4 0.0 0.0 1.7849984269897003 5.2609040127019265E-5 58-59 4.208723210161541E-4 0.0 0.0 2.0405205348866327 5.2609040127019265E-5 60-61 4.208723210161541E-4 0.0 0.0 2.3169021271939285 5.2609040127019265E-5 62-63 4.208723210161541E-4 0.0 0.0 2.5911793578961433 5.2609040127019265E-5 64-65 4.208723210161541E-4 0.0 0.0 2.8766097051052864 5.2609040127019265E-5 66-67 4.208723210161541E-4 0.0 0.0 3.1989979030036606 5.2609040127019265E-5 68-69 4.208723210161541E-4 0.0 0.0 3.5542667509814216 5.2609040127019265E-5 70-71 4.208723210161541E-4 0.0 0.0 3.930000515568593 5.2609040127019265E-5 72-73 4.208723210161541E-4 0.0 0.0 4.320990901792601 5.2609040127019265E-5 74-75 4.208723210161541E-4 0.0 0.0 4.704773849519206 5.2609040127019265E-5 76-77 4.208723210161541E-4 0.0 0.0 5.119596130920753 5.2609040127019265E-5 78-79 4.208723210161541E-4 0.0 0.0 5.5840024326420155 5.2609040127019265E-5 80-81 4.734813611431734E-4 0.0 0.0 6.081447211563047 5.2609040127019265E-5 82-83 4.734813611431734E-4 0.0 0.0 6.607353481192794 1.0521808025403853E-4 84-85 4.734813611431734E-4 0.0 0.0 7.1223170704761225 1.0521808025403853E-4 86-87 4.734813611431734E-4 0.0 0.0 7.660165592214704 1.0521808025403853E-4 88-89 4.734813611431734E-4 0.0 0.0 8.217979244681489 1.0521808025403853E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4200 0.0 56.22406 1 GTATCAA 7975 0.0 44.773434 1 ATCAACG 10520 0.0 34.017487 3 TCAACGC 10620 0.0 33.697174 4 CAACGCA 10720 0.0 33.33762 5 TATCAAC 10775 0.0 33.124218 2 AACGCAG 11050 0.0 32.507202 6 ACGCAGA 12680 0.0 28.138859 7 GTACATG 10145 0.0 27.8756 1 CGCAGAG 12780 0.0 27.807154 8 TACATGG 10185 0.0 27.297182 2 ACATGGG 10600 0.0 25.331772 3 GTATCGA 115 1.2623692E-4 24.839521 1 GCAGAGT 13875 0.0 24.720413 9 TGGTATC 1970 0.0 24.365656 2 CATGGGA 5555 0.0 23.356165 4 GTGTAAG 820 0.0 22.643345 1 GAGTACT 7755 0.0 22.540928 12-13 AGTACTT 8165 0.0 22.10717 12-13 TAAGGTA 560 0.0 22.065222 4 >>END_MODULE