FastQCFastQC Report
Thu 26 May 2016
SRR938546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900814
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94830.4988915275245237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72330.38052118723873035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55840.2937688800692756No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23680.12457820702078164No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21430.11274117299220228No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20350.1070593966584842No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20330.10695417857823017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41650.059.375411
GTATCAA80500.044.426311
ATCAACG108450.032.828673
TATCAAC108900.032.7093732
TCAACGC110550.032.2042124
CAACGCA112250.031.6741395
AACGCAG117200.030.4745246
GTCTACG700.0083436127.175671
GTACATG104800.026.3199441
ACGCAGA136350.026.0551247
TGGTATC19150.025.8275282
TACATGG104800.025.7300152
CGCAGAG137350.025.727118
GCAGAGT147350.023.5620989
ACATGGG107900.023.5245233
AGGTAAG7250.022.9587552
GAGTACT81100.022.81042112-13
CATGGGA57250.021.5867584
GTACTTT84900.021.4817814-15
GTGGTAT24500.021.1581971
Produced by FastQC (version 0.11.5)