##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938546_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1900814 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.798830395819895 31.0 31.0 34.0 30.0 34.0 2 32.016686009257086 31.0 31.0 34.0 30.0 34.0 3 32.0750909873349 33.0 31.0 34.0 30.0 34.0 4 35.75871232009023 37.0 35.0 37.0 35.0 37.0 5 35.627776310570106 37.0 35.0 37.0 33.0 37.0 6 35.66297544104789 37.0 35.0 37.0 33.0 37.0 7 35.596845877608224 37.0 35.0 37.0 33.0 37.0 8 35.58690119075301 37.0 35.0 37.0 33.0 37.0 9 37.15626305361808 39.0 37.0 39.0 33.0 39.0 10-11 37.06911933519008 39.0 37.0 39.0 33.0 39.0 12-13 36.96618580250356 39.0 37.0 39.0 33.0 39.0 14-15 38.00091697556941 40.0 37.0 41.0 33.0 41.0 16-17 37.9379576328878 40.0 37.0 41.0 33.0 41.0 18-19 38.07312314618895 40.0 37.0 41.0 33.0 41.0 20-21 38.12442432557841 40.0 37.0 41.0 33.0 41.0 22-23 38.021094383774525 40.0 37.0 41.0 33.0 41.0 24-25 37.91614802921275 40.0 37.0 41.0 33.0 41.0 26-27 37.725370814819335 40.0 37.0 41.0 32.0 41.0 28-29 37.60471040301681 39.5 37.0 41.0 32.0 41.0 30-31 37.34184933402216 39.0 36.0 41.0 31.5 41.0 32-33 37.10279674918219 39.0 36.0 41.0 31.0 41.0 34-35 36.937380774762815 39.0 35.0 41.0 31.0 41.0 36-37 36.912189988078794 39.0 35.0 41.0 31.0 41.0 38-39 36.78185345857091 39.0 35.0 41.0 30.0 41.0 40-41 36.59460499554402 39.0 35.0 41.0 30.0 41.0 42-43 36.358926491492596 39.0 35.0 40.5 30.0 41.0 44-45 36.0531374979351 39.0 35.0 40.0 29.5 41.0 46-47 35.638510659117614 38.0 35.0 40.0 27.5 41.0 48-49 35.23883162687143 38.0 34.0 40.0 27.0 41.0 50-51 34.86381466045599 38.0 34.0 40.0 26.0 41.0 52-53 34.37469736649667 38.0 33.0 40.0 24.0 41.0 54-55 34.062478759100046 37.5 33.0 40.0 24.0 41.0 56-57 33.728062556357436 37.0 33.0 40.0 23.0 41.0 58-59 33.28782221721852 36.5 32.0 39.5 22.0 41.0 60-61 32.92483062519531 36.0 31.5 39.0 21.5 40.5 62-63 32.47113526099871 35.5 31.0 39.0 19.5 40.0 64-65 31.975792476275952 35.0 31.0 38.5 18.0 40.0 66-67 31.492442185295353 35.0 30.0 38.0 17.0 40.0 68-69 30.96646042169302 34.5 29.5 37.5 13.0 39.5 70-71 30.413489957460328 34.0 29.0 37.0 9.0 39.0 72-73 29.99690106449132 34.0 29.0 36.0 7.5 39.0 74-75 29.51353604297948 34.0 28.5 36.0 7.0 38.5 76-77 28.24058166659126 31.5 26.5 34.5 4.0 36.5 78-79 28.95872268407114 33.5 28.5 35.0 2.0 37.0 80-81 28.71783141327873 33.0 28.0 35.0 2.0 36.5 82-83 28.28329126363758 33.0 27.0 35.0 2.0 36.0 84-85 27.79128126160687 33.0 26.0 35.0 2.0 36.0 86-87 27.26880010353459 32.5 26.0 35.0 2.0 35.0 88-89 26.657551975101192 32.0 25.0 34.5 2.0 35.0 90-91 26.152467574418118 32.0 24.0 34.0 2.0 35.0 92-93 25.46233008595265 31.5 21.5 34.0 2.0 35.0 94-95 24.74193556023893 31.0 19.0 34.0 2.0 35.0 96-97 23.786299974642443 31.0 6.5 34.0 2.0 35.0 98-99 22.599769361968086 30.5 2.0 34.0 2.0 35.0 100-101 18.938337996247924 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37139363787151325 1101 2 0.3488075433110538 1101 3 0.2775803089261828 1101 4 0.17452232692966163 1101 5 0.19996311014104862 1101 6 0.20056294924090423 1101 7 0.28458282031189697 1101 8 0.2746148468213718 1101 9 0.38152672603981586 1101 10-11 0.3925972509015381 1101 12-13 0.41238702725038223 1101 14-15 0.56532016109729 1101 16-17 0.539097940392196 1101 18-19 0.4352382298828914 1101 20-21 0.3797964929867348 1101 22-23 0.44987692686488856 1101 24-25 0.4496400447190112 1101 26-27 0.5022399046266841 1101 28-29 0.4779302691646947 1101 30-31 0.35696568035268683 1101 32-33 0.45749747249649886 1101 34-35 0.3584211417928458 1101 36-37 0.5355683814795 1101 38-39 0.55699745399032 1101 40-41 0.6012857637149622 1101 42-43 0.6764487983378658 1101 44-45 0.7914442853814663 1101 46-47 0.8715145526671435 1101 48-49 0.9372413482944211 1101 50-51 0.9010485930345666 1101 52-53 0.9842176188902698 1101 54-55 0.8237362637200007 1101 56-57 0.8595645120373518 1101 58-59 0.8832055989670522 1101 60-61 0.9723777977667822 1101 62-63 0.9432846080095132 1101 64-65 0.9685464367123764 1101 66-67 0.7533819983821708 1101 68-69 0.8637504884417133 1101 70-71 0.7558282201932904 1101 72-73 0.6552305679590056 1101 74-75 0.7489138533909703 1101 76-77 0.8506636092646751 1101 78-79 0.8767816506949444 1101 80-81 1.0045186949765572 1101 82-83 0.9416270485435483 1101 84-85 0.9442189902348197 1101 86-87 0.8435632477993167 1101 88-89 0.9340078778252874 1101 90-91 1.0051612660204725 1101 92-93 1.1291069564507268 1101 94-95 1.3348000807839462 1101 96-97 1.1188027326784713 1101 98-99 0.8526759506813271 1101 100-101 0.8093727591179878 1102 1 0.3415081155462758 1102 2 0.3112618570330383 1102 3 0.3893762241841259 1102 4 0.3009174895817637 1102 5 0.1585501293578062 1102 6 0.1796933907394731 1102 7 0.16955819158586394 1102 8 0.24267947585634175 1102 9 0.3763117060802088 1102 10-11 0.3402534408370883 1102 12-13 0.43202155106868645 1102 14-15 0.5637565327633851 1102 16-17 0.3847833758647923 1102 18-19 0.38829515136059456 1102 20-21 0.4378238147062419 1102 22-23 0.41072092106114155 1102 24-25 0.4271413848966361 1102 26-27 0.37949262298435116 1102 28-29 0.491792462744975 1102 30-31 0.4222210092043639 1102 32-33 0.2974079447691196 1102 34-35 0.31138555854087 1102 36-37 0.39186535571474224 1102 38-39 0.4283002049440725 1102 40-41 0.5018143922612595 1102 42-43 0.44255998024640064 1102 44-45 0.4584653487259729 1102 46-47 0.49391867160260006 1102 48-49 0.6719293581016785 1102 50-51 0.7117558311873751 1102 52-53 0.696882657333262 1102 54-55 0.6670936342405867 1102 56-57 0.604515631976497 1102 58-59 0.6893142813170385 1102 60-61 0.8443426508843643 1102 62-63 0.48930088392707205 1102 64-65 0.6111250121957035 1102 66-67 0.41622191815758214 1102 68-69 0.36787353131721545 1102 70-71 0.2720081224085895 1102 72-73 0.22238162941128437 1102 74-75 0.2691465861632416 1102 76-77 0.31956815316018705 1102 78-79 0.605216551412969 1102 80-81 0.5358904365545065 1102 82-83 0.477073562028 1102 84-85 0.5036951431474179 1102 86-87 0.30685343917351915 1102 88-89 0.5811342807112965 1102 90-91 0.5936721434883943 1102 92-93 0.8095815776815982 1102 94-95 0.6576191194158163 1102 96-97 0.6474035451052096 1102 98-99 0.4715466346862236 1102 100-101 0.67339905643977 1103 1 0.2996106442225539 1103 2 0.29511793150282983 1103 3 0.33039074964383275 1103 4 0.19759189873662564 1103 5 0.18047447766006286 1103 6 0.13913418402751887 1103 7 0.3041999627202756 1103 8 0.2724077398144189 1103 9 0.3004175833744185 1103 10-11 0.2536775094308723 1103 12-13 0.46071950895046854 1103 14-15 0.5812773203751362 1103 16-17 0.6056885210224863 1103 18-19 0.5659970810041415 1103 20-21 0.4775282927682625 1103 22-23 0.376279495431568 1103 24-25 0.4575377956932911 1103 26-27 0.4861430163007867 1103 28-29 0.4465141815576388 1103 30-31 0.5766493215488921 1103 32-33 0.6257822423362356 1103 34-35 0.5262678559673901 1103 36-37 0.6864023191154658 1103 38-39 0.5579704577262916 1103 40-41 0.7046725102617728 1103 42-43 0.6109750301718151 1103 44-45 0.6534001452811751 1103 46-47 0.6869952052935915 1103 48-49 0.7219464231106656 1103 50-51 0.605496668394423 1103 52-53 0.7848441557823094 1103 54-55 0.8112412230812893 1103 56-57 0.7540083955257728 1103 58-59 0.7980499234688949 1103 60-61 0.7995814115475355 1103 62-63 0.8292769239317934 1103 64-65 0.6851483894513173 1103 66-67 0.6906008191709994 1103 68-69 0.497043330948717 1103 70-71 0.3155557587488147 1103 72-73 0.1585759057514302 1103 74-75 0.44942452736434646 1103 76-77 0.45270610117415444 1103 78-79 0.5446579425511899 1103 80-81 0.5178862772254611 1103 82-83 0.415490067160146 1103 84-85 0.5334574057278125 1103 86-87 0.7362008528638313 1103 88-89 0.6240347935156336 1103 90-91 0.7467150530346487 1103 92-93 0.6147123147027465 1103 94-95 0.7658341063370209 1103 96-97 0.5102225531929392 1103 98-99 0.5786081321169441 1103 100-101 0.3612892399042238 1104 1 0.30431947689631755 1104 2 0.30487587084083145 1104 3 0.3373006422943874 1104 4 0.2372296234689486 1104 5 0.17771033414834392 1104 6 0.12578425651054204 1104 7 0.16364070144112475 1104 8 0.21085543783756577 1104 9 0.29781005912406755 1104 10-11 0.342113787299553 1104 12-13 0.2865216000040576 1104 14-15 0.3649893833211735 1104 16-17 0.407394086133678 1104 18-19 0.4520635602870442 1104 20-21 0.3759467179056557 1104 22-23 0.4248459164483833 1104 24-25 0.4245587202130565 1104 26-27 0.40499372924471544 1104 28-29 0.4986865746160518 1104 30-31 0.6078421916434635 1104 32-33 0.4398257694392953 1104 34-35 0.5406423344858382 1104 36-37 0.6209284759305049 1104 38-39 0.5915009635226056 1104 40-41 0.5862044890249436 1104 42-43 0.6040199230321193 1104 44-45 0.6372306049307426 1104 46-47 0.622537522792534 1104 48-49 0.8100508173064256 1104 50-51 0.6573092100546774 1104 52-53 0.5285204200400173 1104 54-55 0.632522797477769 1104 56-57 0.6427187571018464 1104 58-59 0.7533663549736076 1104 60-61 0.4733327691475253 1104 62-63 0.5552020729764635 1104 64-65 0.6075029922397057 1104 66-67 0.45820766753465847 1104 68-69 0.41761545738147987 1104 70-71 0.3662188269014983 1104 72-73 0.198153966003229 1104 74-75 0.3251974853708077 1104 76-77 0.14771898963420682 1104 78-79 0.20182910036839985 1104 80-81 0.1595199652827759 1104 82-83 0.1631186371237412 1104 84-85 0.1423837713685323 1104 86-87 0.27043894210883934 1104 88-89 0.23016158985932123 1104 90-91 0.49407373444962843 1104 92-93 0.3429806929941961 1104 94-95 0.3264285137242311 1104 96-97 0.30765791278796684 1104 98-99 0.33046402375133255 1104 100-101 0.24432261938430244 1105 1 0.13936204131964303 1105 2 0.23474371928575977 1105 3 0.12941857169821702 1105 4 0.11662702302444927 1105 5 0.12900190599327033 1105 6 0.13518879819439888 1105 7 0.11435824135497796 1105 8 0.18615964370830795 1105 9 0.10005029193083459 1105 10-11 0.19706204013975537 1105 12-13 0.26429760334001173 1105 14-15 0.2773559120249871 1105 16-17 0.33561214728981525 1105 18-19 0.24609522520145077 1105 20-21 0.178083589475996 1105 22-23 0.11182284426852362 1105 24-25 0.17194962531147695 1105 26-27 0.19929466570638965 1105 28-29 0.21489033377106637 1105 30-31 0.19832142401993025 1105 32-33 0.31264176425791845 1105 34-35 0.39923351639718163 1105 36-37 0.38458302759559615 1105 38-39 0.14637380258516686 1105 40-41 0.08103997608704816 1105 42-43 0.21370083403055418 1105 44-45 0.2250805581298323 1105 46-47 0.01823050886759603 1105 48-49 0.02308453613724737 1105 50-51 0.01574867718979789 1105 52-53 0.194175211254759 1105 54-55 0.3391831648759478 1105 56-57 0.4115990399478733 1105 58-59 0.2965922014932616 1105 60-61 0.11189493893500213 1105 62-63 0.16355437065681855 1105 64-65 0.25739009337845076 1105 66-67 0.007455082394724144 1105 68-69 -0.0057113548781373424 1105 70-71 0.13737172611763881 1105 72-73 0.03439161699714077 1105 74-75 0.049947399975796 1105 76-77 -0.02358207547449709 1105 78-79 0.0537435012946581 1105 80-81 0.2589703319268182 1105 82-83 0.41105695752583316 1105 84-85 0.22263157216969276 1105 86-87 0.3973949315154215 1105 88-89 0.24509928477066723 1105 90-91 0.054271229824990996 1105 92-93 0.2905122226074148 1105 94-95 0.1686163117750077 1105 96-97 0.29016102915142383 1105 98-99 -0.0876539145247115 1105 100-101 -0.06831096281449689 1106 1 0.1793201314208268 1106 2 0.151883530232098 1106 3 0.1660310683752968 1106 4 0.1530280997358915 1106 5 0.059037768034094995 1106 6 0.01737318021044132 1106 7 0.16596993599652876 1106 8 0.15742296585848692 1106 9 0.0935280143313193 1106 10-11 0.1363072821986151 1106 12-13 0.11286068442608865 1106 14-15 0.18623815790583365 1106 16-17 0.015705965608084682 1106 18-19 0.3051104446049351 1106 20-21 0.22140824495497213 1106 22-23 0.36836496020261933 1106 24-25 0.28798985405314426 1106 26-27 0.10348599567401351 1106 28-29 0.24504261849538267 1106 30-31 0.21285541303856093 1106 32-33 0.17176918361367655 1106 34-35 -0.03437664672493668 1106 36-37 -0.24759398589616666 1106 38-39 -0.15176703371966482 1106 40-41 0.21968414494867972 1106 42-43 0.38765142864212265 1106 44-45 0.2046198666561807 1106 46-47 -0.21235328797425268 1106 48-49 -0.2072718283689099 1106 50-51 -0.13925729981700385 1106 52-53 -0.1477756112142572 1106 54-55 -0.4597680673963751 1106 56-57 -0.07441436736544915 1106 58-59 -0.03372060369825647 1106 60-61 -0.414133522840217 1106 62-63 -0.21866203609932455 1106 64-65 -0.6212904136440365 1106 66-67 -0.1710427419067848 1106 68-69 -0.94917847793986 1106 70-71 -0.7095250765663152 1106 72-73 -0.40644225055420335 1106 74-75 -0.11372126922904258 1106 76-77 -0.07957308247403105 1106 78-79 -0.2611053220937194 1106 80-81 -0.943339870475203 1106 82-83 -0.35758470036945766 1106 84-85 -0.18922118090936024 1106 86-87 -0.16221019493695366 1106 88-89 0.024127019329213795 1106 90-91 0.005900410541450185 1106 92-93 -0.24198226091854735 1106 94-95 -0.23759852831951278 1106 96-97 -0.3250873666358203 1106 98-99 -0.38460487586206327 1106 100-101 -0.7690801517270209 1107 1 0.1262679829357296 1107 2 0.17455527437191165 1107 3 0.1773629324762922 1107 4 0.10062257023510313 1107 5 0.025912172182714244 1107 6 0.07362529107406601 1107 7 0.08394551624354563 1107 8 0.06518731011202306 1107 9 0.15245477644820937 1107 10-11 0.12862213541076528 1107 12-13 0.10099389007443449 1107 14-15 0.26592513018992747 1107 16-17 0.36170002740134777 1107 18-19 0.2368480541026372 1107 20-21 0.2302487555808952 1107 22-23 0.2842411663340627 1107 24-25 0.2634290205666119 1107 26-27 0.3308382993476542 1107 28-29 0.3346378243232877 1107 30-31 0.24874249946960703 1107 32-33 0.23510925389742 1107 34-35 0.15089679294604252 1107 36-37 0.10494856270178587 1107 38-39 0.10435019563020376 1107 40-41 0.14672174290344486 1107 42-43 0.20750150922901867 1107 44-45 0.1679426759538103 1107 46-47 0.13451290785403813 1107 48-49 0.04548511984599202 1107 50-51 -0.046101414408582286 1107 52-53 0.12390456141697825 1107 54-55 0.17621724099279135 1107 56-57 0.20540599012400662 1107 58-59 0.032916689009582 1107 60-61 -0.027898117191007543 1107 62-63 -0.011852443479973829 1107 64-65 0.14901769763000416 1107 66-67 0.2512440768988178 1107 68-69 0.21742236897068778 1107 70-71 0.038662252694205534 1107 72-73 -0.0327878593165849 1107 74-75 -0.038740988436085644 1107 76-77 0.07298303977864151 1107 78-79 0.21239612505025818 1107 80-81 0.3744474421121673 1107 82-83 0.19234817951118188 1107 84-85 0.2471752916504819 1107 86-87 0.3304250285901418 1107 88-89 0.25666670356539356 1107 90-91 0.40846641911743475 1107 92-93 0.41063050729473005 1107 94-95 0.35843588036078344 1107 96-97 0.20160330153000316 1107 98-99 0.07847611931127574 1107 100-101 -0.1596738227700314 1108 1 0.23223029371732196 1108 2 0.19657655788653727 1108 3 0.19834564876655492 1108 4 0.13540745877712368 1108 5 0.1938409724038408 1108 6 0.18557714708109074 1108 7 0.29830497838360515 1108 8 0.15420735791457219 1108 9 0.25930728770782707 1108 10-11 0.17188120407408292 1108 12-13 0.18625192626779352 1108 14-15 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2316 68-69 -0.7182475757778448 2316 70-71 -0.7059672531724637 2316 72-73 -0.6557405540453267 2316 74-75 -0.485092315778477 2316 76-77 -0.3727771787969729 2316 78-79 -0.3616396438767282 2316 80-81 -0.6314267084494602 2316 82-83 -0.6464435605694234 2316 84-85 -0.5540631485311991 2316 86-87 -0.4102558891254944 2316 88-89 -0.2850128508143115 2316 90-91 -0.33735715637602937 2316 92-93 -0.3846182531834401 2316 94-95 -0.4177884293640979 2316 96-97 -0.0796879506586059 2316 98-99 0.013670139228313616 2316 100-101 0.05696886354487063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 33.0 5 70.0 6 165.0 7 388.0 8 954.0 9 1623.0 10 2650.0 11 4190.0 12 6043.0 13 8209.0 14 10350.0 15 13776.0 16 17697.0 17 22380.0 18 20533.0 19 16352.0 20 15585.0 21 16343.0 22 17592.0 23 19961.0 24 22779.0 25 27148.0 26 32678.0 27 39418.0 28 47599.0 29 57612.0 30 68346.0 31 81417.0 32 98315.0 33 122572.0 34 157366.0 35 208657.0 36 274477.0 37 309846.0 38 151276.0 39 6400.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7501730846526 19.507376878415663 15.089456882456057 22.652993154475684 2 15.855387162942092 20.955134038894467 39.17874958088995 24.010729217273493 3 18.927514166411072 25.328190934443885 30.03268625456979 25.711608644575257 4 13.161519222817173 17.105776788260187 36.100428553240874 33.63227543568176 5 13.486452574929563 38.14440372005937 33.64117949660307 14.727964208407993 6 32.10745501664024 36.36331592675559 16.948265322119894 14.580963734484278 7 28.889780904391486 33.283056627318615 20.166255088609404 17.660907379680495 8 27.146422532662324 34.624166278236586 19.36049503002398 18.868916159077113 9 27.25621759940741 16.284339235716907 19.701138564846428 36.75830460002925 10-11 25.247709665438073 26.871987474839727 26.607390307520884 21.27291255220132 12-13 26.254620388949157 24.264788664224906 27.29443806705969 22.186152879766247 14-15 23.01540287476839 25.369683724972564 25.769775475138545 25.845137925120497 16-17 21.949543721794978 28.708832110874603 26.67173116359623 22.66989300373419 18-19 22.114762412313883 26.666575477663777 29.546262811616497 21.672399298405843 20-21 22.804756278099802 25.7987893607686 29.73352468994862 21.662929671182976 22-23 22.62572771454756 25.721532985342073 29.751622199752315 21.901117100358057 24-25 22.332517364974596 26.138186424832792 29.74249385393272 21.786802356259887 26-27 21.755342335372205 26.450909915510195 29.599133135698512 22.194614613419088 28-29 21.881809582631444 26.474157913399203 29.637828845957575 22.006203658011778 30-31 21.923423333371915 26.256303878233222 29.340745596360296 22.47952719203457 32-33 21.655196142284304 26.36046977768472 29.306760156438244 22.677573923592735 34-35 22.465979092874026 26.454833115506926 29.091348187621147 21.987839603997905 36-37 22.600569599126267 25.915813280703155 29.118289277126784 22.365327843043797 38-39 21.99803391690629 26.279591536635728 29.224822943353185 22.4975516031048 40-41 22.328504171731662 26.148577675862615 29.366849589587467 22.156068562818255 42-43 22.165690677696368 26.150866447777183 28.98860852259067 22.694834351935782 44-45 22.02861510910589 26.404977025632174 29.14732846033331 22.419079404928624 46-47 22.617625922367996 26.67683424048855 28.356640891744274 22.34889894539918 48-49 22.296737082113243 27.007429448646736 28.014392781197948 22.68144068804207 50-51 22.500702330685694 26.895608933856757 28.02378349486062 22.579905240596922 52-53 22.806413462863805 26.64148096552319 28.029044398873324 22.523061172739677 54-55 22.30368147541001 27.029235895779387 27.978539720351385 22.688542908459215 56-57 22.162294680068644 27.186852579999936 27.800615946641805 22.85023679328961 58-59 22.16295229307023 26.652607777510056 28.015287134880108 23.169152794539603 60-61 21.840169527370907 27.073822057287032 28.135472487050283 22.950535928291774 62-63 22.049500898036314 26.973207268044114 28.39344091531312 22.583850918606448 64-65 21.88849093072757 26.971865737520872 28.046510600195496 23.09313273155606 66-67 21.675862295728802 27.140656955907012 27.938763028967877 23.24471771939631 68-69 22.282568380670593 26.76638118932467 27.996972842387365 22.954077587617377 70-71 22.224344122406634 26.659764353339522 27.68477366514942 23.431117859104425 72-73 22.03246136456838 26.973687844704386 27.533500787952104 23.460350002775126 74-75 21.96874602144134 27.299909407232903 27.447504069309254 23.283840502016503 76-77 21.981003927790933 26.854810623238258 27.892024153862504 23.272161295108308 78-79 22.000495577158 27.071270518840873 27.715389301636034 23.21284460236509 80-81 22.12753062475849 27.0461947279303 27.371796102726737 23.454478544584475 82-83 22.155133705158157 26.909328703015646 27.52732986094579 23.40820773088041 84-85 21.83387251469311 27.02448176244699 27.520924954267358 23.620720768592548 86-87 21.97373858589674 27.014212898064923 27.318325934152597 23.693722581885744 88-89 21.921900198941348 27.018907450844022 27.11234122899614 23.94685112121849 90-91 21.64613318637438 26.875443079929973 27.49782652442513 23.980597209270517 92-93 21.800779566908442 26.622988543238925 27.411262316531097 24.164969573321535 94-95 21.5685340956738 26.26686580952466 27.755965473720984 24.40863462108056 96-97 21.827911621021308 25.931863927769893 27.62697980970258 24.613244641506217 98-99 21.46220180170417 26.251945550624033 27.430073944733607 24.855778702938192 100-101 21.41312127675861 25.96412009910494 27.80046543230043 24.822293191836025 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1101.0 1 732.5 2 501.0 3 642.5 4 552.0 5 529.0 6 971.5 7 2127.5 8 3328.0 9 3617.0 10 2977.0 11 2384.5 12 2318.0 13 2358.0 14 2445.5 15 2592.0 16 2696.5 17 2804.5 18 3001.0 19 3255.5 20 3584.0 21 4012.0 22 4662.5 23 5510.5 24 6490.0 25 7748.0 26 9527.5 27 11699.0 28 13659.5 29 16020.5 30 19069.5 31 23572.0 32 30781.5 33 34602.0 34 34946.5 35 37811.5 36 43883.0 37 49305.5 38 52742.0 39 55466.0 40 56621.0 41 57797.5 42 58751.5 43 60453.5 44 62619.0 45 65444.0 46 65736.0 47 65582.5 48 66823.0 49 66533.0 50 66002.0 51 64480.0 52 63011.5 53 64141.0 54 66298.5 55 63459.5 56 58481.0 57 53974.0 58 50698.0 59 46404.0 60 38105.5 61 31625.0 62 26849.0 63 19877.0 64 13720.0 65 10053.5 66 7419.5 67 5905.5 68 4683.0 69 3701.0 70 2908.5 71 1939.0 72 1369.0 73 1147.0 74 870.0 75 636.5 76 475.0 77 294.5 78 186.5 79 128.0 80 69.5 81 25.0 82 13.0 83 9.0 84 6.0 85 5.5 86 3.5 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.260904012701926E-4 2 0.0502416333213034 3 7.89135601905289E-4 4 0.0 5 0.08401663708284977 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.630452006350963E-4 26-27 0.008969841341656785 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0114687707476902 36-37 1.8413164044456742E-4 38-39 0.004235027730225051 40-41 0.0054976446932735136 42-43 3.6826328088913484E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.89135601905289E-5 68-69 0.0011047898426674045 70-71 1.0521808025403853E-4 72-73 2.6304520063509633E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012363124429849528 82-83 4.4717684107966374E-4 84-85 0.004892640731812791 86-87 0.00213066612514428 88-89 1.3152260031754816E-4 90-91 0.0029724107671765883 92-93 7.89135601905289E-4 94-95 5.2609040127019265E-5 96-97 0.0 98-99 1.3152260031754816E-4 100-101 2.6304520063509633E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1900814.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.4345974346791 #Duplication Level Percentage of deduplicated Percentage of total 1 71.36693025271896 30.99793885675669 2 14.385387777359504 12.496470541027263 3 5.436848986163135 7.084420410814168 4 2.749075452797662 4.776199424392984 5 1.4769834330476144 3.207609041605672 6 0.9456734372736814 2.464496703159096 7 0.6364577522932455 1.9351000378526482 8 0.4632612695626986 1.6097253400427336 9 0.33182061942742236 1.2971245522820352 >10 1.9139276214125867 15.701881889742065 >50 0.17833873135602996 5.367363063029445 >100 0.10545471758297519 8.08812049236895 >500 0.006681446854915266 1.9559353681017886 >1k 0.0027940595938736566 1.8409610454307213 >5k 3.6444255572265087E-4 1.1766532333936681 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9483 0.4988915275245237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7233 0.38052118723873035 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5584 0.2937688800692756 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2368 0.12457820702078164 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2143 0.11274117299220228 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2035 0.1070593966584842 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2033 0.10695417857823017 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.2609040127019265E-5 0.0 16-17 0.0 0.0 0.0 1.0521808025403853E-4 0.0 18-19 0.0 0.0 0.0 1.0521808025403853E-4 0.0 20-21 0.0 0.0 0.0 1.578271203810578E-4 0.0 22-23 0.0 0.0 0.0 4.99785881206683E-4 0.0 24-25 5.2609040127019265E-5 0.0 0.0 0.0013941395633660105 0.0 26-27 5.2609040127019265E-5 0.0 0.0 0.002814583646795531 0.0 28-29 5.2609040127019265E-5 0.0 0.0 0.006286780295178803 0.0 30-31 5.2609040127019265E-5 0.0 0.0 0.019833608127886263 0.0 32-33 5.2609040127019265E-5 0.0 0.0 0.04584877847069729 0.0 34-35 5.2609040127019265E-5 0.0 0.0 0.08567382184685088 0.0 36-37 1.0521808025403853E-4 0.0 0.0 0.15164555816613304 0.0 38-39 1.0521808025403853E-4 0.0 0.0 0.25433840449407463 0.0 40-41 1.0521808025403853E-4 0.0 0.0 0.365895874083419 0.0 42-43 1.0521808025403853E-4 0.0 0.0 0.4781109566743511 0.0 44-45 1.0521808025403853E-4 0.0 0.0 0.6171303452099994 2.6304520063509633E-5 46-47 1.0521808025403853E-4 0.0 0.0 0.7818229453276333 5.2609040127019265E-5 48-49 1.0521808025403853E-4 0.0 0.0 0.958589320154418 5.2609040127019265E-5 50-51 1.0521808025403853E-4 0.0 0.0 1.1587404133176629 5.2609040127019265E-5 52-53 1.0521808025403853E-4 0.0 0.0 1.354472347110238 5.2609040127019265E-5 54-55 1.0521808025403853E-4 0.0 0.0 1.5570171515992621 5.2609040127019265E-5 56-57 1.0521808025403853E-4 0.0 0.0 1.79294239204888 5.2609040127019265E-5 58-59 1.0521808025403853E-4 0.0 0.0 2.0483855863856224 5.2609040127019265E-5 60-61 1.0521808025403853E-4 0.0 0.0 2.3246882651327274 5.2609040127019265E-5 62-63 1.0521808025403853E-4 0.0 0.0 2.6000176766374827 5.2609040127019265E-5 64-65 1.0521808025403853E-4 0.0 0.0 2.886842163409992 1.0521808025403853E-4 66-67 1.0521808025403853E-4 0.0 0.0 3.2099142788300172 1.0521808025403853E-4 68-69 1.0521808025403853E-4 0.0 0.0 3.5675768381335575 1.0521808025403853E-4 70-71 1.0521808025403853E-4 0.0 0.0 3.946677581288858 1.0521808025403853E-4 72-73 1.0521808025403853E-4 0.0 0.0 4.341797777162837 1.0521808025403853E-4 74-75 1.0521808025403853E-4 0.0 0.0 4.730710106301826 1.0521808025403853E-4 76-77 1.0521808025403853E-4 0.0 0.0 5.152634608120521 1.0521808025403853E-4 78-79 1.0521808025403853E-4 0.0 0.0 5.624564002579947 1.0521808025403853E-4 80-81 2.630452006350963E-4 0.0 0.0 6.131872976524794 1.0521808025403853E-4 82-83 2.630452006350963E-4 0.0 0.0 6.667748659258612 1.0521808025403853E-4 84-85 3.156542407621156E-4 0.0 0.0 7.196522121575283 1.0521808025403853E-4 86-87 3.682632808891349E-4 0.0 0.0 7.748364647987652 1.0521808025403853E-4 88-89 3.682632808891349E-4 0.0 0.0 8.320619481969302 1.0521808025403853E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4165 0.0 59.37541 1 GTATCAA 8050 0.0 44.42631 1 ATCAACG 10845 0.0 32.82867 3 TATCAAC 10890 0.0 32.709373 2 TCAACGC 11055 0.0 32.204212 4 CAACGCA 11225 0.0 31.674139 5 AACGCAG 11720 0.0 30.474524 6 GTCTACG 70 0.00834361 27.17567 1 GTACATG 10480 0.0 26.319944 1 ACGCAGA 13635 0.0 26.055124 7 TGGTATC 1915 0.0 25.827528 2 TACATGG 10480 0.0 25.730015 2 CGCAGAG 13735 0.0 25.72711 8 GCAGAGT 14735 0.0 23.562098 9 ACATGGG 10790 0.0 23.524523 3 AGGTAAG 725 0.0 22.958755 2 GAGTACT 8110 0.0 22.810421 12-13 CATGGGA 5725 0.0 21.586758 4 GTACTTT 8490 0.0 21.48178 14-15 GTGGTAT 2450 0.0 21.158197 1 >>END_MODULE