FastQCFastQC Report
Thu 26 May 2016
SRR938545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901523
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98650.5187946714291649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69320.3645498897462718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53530.28151118866298225No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24390.12826560604315593No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23140.12169192799666373No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21480.11296208355092209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.059.35171
GTATCAA77650.047.2697561
ATCAACG106650.034.358473
TATCAAC107100.034.3028642
TCAACGC108400.033.891484
CAACGCA110800.033.2860535
AACGCAG115550.031.994956
ACGCAGA131750.027.8761047
CGCAGAG132850.027.5737618
GTACATG103550.027.2524571
TACATGG103550.027.1254982
GTATAGG5650.026.1545521
GTGTAAG8350.025.6897641
GCAGAGT142350.025.0653769
ACATGGG108450.024.4975473
TGGTATC16350.023.8361682
TAAGGTA5250.023.5371784
GAGTACT77850.022.48606712-13
AGAGTAC130150.022.3381710-11
AGTACTT82450.022.2686312-13
Produced by FastQC (version 0.11.5)