##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938545_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1901523 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16034305133306 31.0 31.0 34.0 28.0 34.0 2 31.291568390179872 31.0 31.0 34.0 28.0 34.0 3 31.34034034823665 31.0 31.0 34.0 28.0 34.0 4 35.03132489062715 37.0 35.0 37.0 32.0 37.0 5 35.04332106422063 37.0 35.0 37.0 32.0 37.0 6 35.06088540606661 37.0 35.0 37.0 32.0 37.0 7 34.9826207729278 37.0 35.0 37.0 32.0 37.0 8 34.94138435348928 37.0 35.0 37.0 32.0 37.0 9 36.430621664844445 39.0 37.0 39.0 32.0 39.0 10-11 36.28799835710639 39.0 35.0 39.0 32.0 39.0 12-13 36.132648145723195 39.0 35.0 39.0 31.0 39.0 14-15 37.11165129214845 39.0 36.0 41.0 31.0 41.0 16-17 36.96338435033392 39.0 36.0 41.0 31.0 41.0 18-19 36.87985998591655 39.0 36.0 41.0 31.0 41.0 20-21 36.877434561664515 39.0 36.0 40.5 30.5 41.0 22-23 36.96069545306578 39.0 36.0 41.0 31.0 41.0 24-25 36.87135943136107 39.0 36.0 41.0 30.0 41.0 26-27 36.67674621868891 39.0 36.0 41.0 30.0 41.0 28-29 36.50712271163694 39.0 36.0 41.0 30.0 41.0 30-31 36.225376185299886 39.0 35.0 40.0 29.5 41.0 32-33 35.90823776520189 38.0 35.0 40.0 28.5 41.0 34-35 35.65287666780786 38.0 35.0 40.0 27.0 41.0 36-37 35.363643248070105 38.0 34.0 40.0 26.5 41.0 38-39 35.045414386257754 38.0 34.0 40.0 25.5 41.0 40-41 34.929308769864996 38.0 34.0 40.0 25.0 41.0 42-43 34.68639480037844 38.0 33.5 40.0 24.5 41.0 44-45 34.35780003712813 38.0 33.0 40.0 24.0 41.0 46-47 33.930538047659695 38.0 33.0 40.0 22.5 41.0 48-49 33.51068380450828 38.0 32.5 40.0 20.5 41.0 50-51 32.71240579261992 36.5 31.5 39.5 18.5 40.5 52-53 32.73004297081866 37.0 31.5 39.0 18.0 40.0 54-55 32.81711054770308 37.0 31.5 40.0 16.5 41.0 56-57 32.501942390389175 36.5 31.0 39.5 13.5 41.0 58-59 32.078932518828324 36.0 31.0 39.0 9.0 41.0 60-61 31.61152376279435 35.5 30.5 39.0 7.5 40.5 62-63 31.0431788098277 35.0 29.5 39.0 4.5 40.0 64-65 30.54852426186799 35.0 29.0 38.0 2.0 40.0 66-67 30.007985704090878 34.5 28.0 38.0 2.0 40.0 68-69 29.410689484166113 34.0 27.5 37.0 2.0 39.5 70-71 28.79336300428656 34.0 26.0 36.5 2.0 39.0 72-73 28.168619574940717 33.5 26.0 36.0 2.0 39.0 74-75 27.49354885531229 33.0 25.5 35.5 2.0 38.5 76-77 26.78592212663218 32.5 23.5 35.0 2.0 37.5 78-79 26.02731415817742 32.0 21.0 35.0 2.0 37.0 80-81 25.301005825330538 31.5 19.0 35.0 2.0 36.0 82-83 24.589630785428312 31.0 15.5 34.5 2.0 36.0 84-85 23.86892164859431 31.0 7.5 34.0 2.0 35.0 86-87 23.16939342832035 30.0 2.0 34.0 2.0 35.0 88-89 22.390305034438185 29.5 2.0 34.0 2.0 35.0 90-91 21.57560439710695 29.0 2.0 34.0 2.0 35.0 92-93 20.70607218529568 28.5 2.0 34.0 2.0 35.0 94-95 19.797972204385644 26.5 2.0 34.0 2.0 35.0 96-97 18.71754167580408 25.0 2.0 33.0 2.0 35.0 98-99 17.459336279392886 22.0 2.0 33.0 2.0 35.0 100-101 14.614193727869713 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5718907132620075 1101 2 0.48754154583843956 1101 3 0.47703677154100177 1101 4 0.45455489719343944 1101 5 0.4517058124438975 1101 6 0.385567851922346 1101 7 0.2423444603021565 1101 8 0.288143001347656 1101 9 0.531883446132241 1101 10-11 0.4527846624271916 1101 12-13 0.5652075187193404 1101 14-15 0.684038502864631 1101 16-17 0.8276610882930413 1101 18-19 0.8862705302774572 1101 20-21 1.0091595467163117 1101 22-23 1.0119451914848057 1101 24-25 1.0613181039651067 1101 26-27 0.9800027397286968 1101 28-29 1.0261486284192287 1101 30-31 0.9437267831404696 1101 32-33 0.9667823950385639 1101 34-35 0.9731151593483816 1101 36-37 1.0856192194564898 1101 38-39 1.057541893511889 1101 40-41 1.1284461190443338 1101 42-43 1.1180284924455108 1101 44-45 0.9548148762247379 1101 46-47 1.1590411071205438 1101 48-49 1.0760878450746887 1101 50-51 1.0724119096152904 1101 52-53 1.2227137337110463 1101 54-55 1.341982750754255 1101 56-57 1.307497507365781 1101 58-59 1.4874093328706834 1101 60-61 1.603920601215119 1101 62-63 1.3837800427767597 1101 64-65 1.470651835999302 1101 66-67 1.5790538576997015 1101 68-69 1.7239450543698034 1101 70-71 1.8620002401765952 1101 72-73 2.1173619696928974 1101 74-75 2.2550311163664922 1101 76-77 2.481153331008887 1101 78-79 2.704102082706772 1101 80-81 2.901926401391677 1101 82-83 3.1686595166169838 1101 84-85 3.227793832363222 1101 86-87 3.415216885533237 1101 88-89 3.765280596326864 1101 90-91 4.155455233166496 1101 92-93 4.159751012564339 1101 94-95 4.541424954346166 1101 96-97 4.760375773509946 1101 98-99 5.146511488153482 1101 100-101 5.12826588739218 1102 1 0.6678444082581905 1102 2 0.6361699268625891 1102 3 0.5669258113602211 1102 4 0.5568951617120348 1102 5 0.6120677074955978 1102 6 0.6082914161579751 1102 7 0.5766414257581118 1102 8 0.6528202373594212 1102 9 0.6672141260469999 1102 10-11 0.7705769210732285 1102 12-13 0.7611161457071631 1102 14-15 1.0936354555495527 1102 16-17 1.1144102494686976 1102 18-19 1.1742408550520906 1102 20-21 1.128908516854274 1102 22-23 0.8928041645041631 1102 24-25 0.8460919776079265 1102 26-27 0.880763935223591 1102 28-29 0.852864247175539 1102 30-31 1.0254735009334652 1102 32-33 1.0074106240769325 1102 34-35 1.022835621596272 1102 36-37 1.1047024074006373 1102 38-39 0.997004581528337 1102 40-41 1.1873485283357326 1102 42-43 1.3520100873886065 1102 44-45 1.416927668678646 1102 46-47 1.5627127249353947 1102 48-49 1.442674930293336 1102 50-51 1.6702881193007997 1102 52-53 1.619883737204617 1102 54-55 1.7038603874174498 1102 56-57 1.7680049499198986 1102 58-59 1.811511640416036 1102 60-61 1.767608240865254 1102 62-63 2.096748546888289 1102 64-65 2.2114458773119914 1102 66-67 2.2192808020359642 1102 68-69 2.4670304925480018 1102 70-71 2.530112355027189 1102 72-73 2.4976277759511234 1102 74-75 2.6059847744216853 1102 76-77 2.860486113393737 1102 78-79 3.3593940906818 1102 80-81 3.963930270696565 1102 82-83 4.046438138760255 1102 84-85 4.11077374596352 1102 86-87 4.4243534479313205 1102 88-89 4.78291939154396 1102 90-91 5.05311971820381 1102 92-93 5.09663680263186 1102 94-95 5.21498692074675 1102 96-97 5.3662950922758625 1102 98-99 5.689996311211413 1102 100-101 5.536373590433056 1103 1 0.6117791131991837 1103 2 0.627692419433302 1103 3 0.3779763184274998 1103 4 0.4598207941974195 1103 5 0.5005431578159119 1103 6 0.5686537496859287 1103 7 0.5424369471091879 1103 8 0.4236613226659145 1103 9 0.6248013792317408 1103 10-11 0.5893587293728615 1103 12-13 0.6732266689287982 1103 14-15 0.5803126497739228 1103 16-17 0.7874534497655645 1103 18-19 0.9914606722719057 1103 20-21 0.8287717796106193 1103 22-23 0.8555054511048255 1103 24-25 0.9610556303074276 1103 26-27 0.982121929663343 1103 28-29 0.9222220964076016 1103 30-31 0.9805095356340701 1103 32-33 0.9489907459505531 1103 34-35 0.9802585077403307 1103 36-37 1.179957302362034 1103 38-39 1.0924004892932189 1103 40-41 0.9946564079706519 1103 42-43 1.342046217885958 1103 44-45 1.0766788189958305 1103 46-47 1.2079708366505244 1103 48-49 1.2483966175663852 1103 50-51 1.4500947712248475 1103 52-53 1.597112851750289 1103 54-55 1.6039267392657734 1103 56-57 1.6776176991552845 1103 58-59 1.6929409883024036 1103 60-61 2.040927436616318 1103 62-63 2.2806208125215655 1103 64-65 2.37335951302018 1103 66-67 2.3371828937325994 1103 68-69 2.5639505768894644 1103 70-71 2.6489028354528905 1103 72-73 2.8737162034022354 1103 74-75 3.2377799146025694 1103 76-77 3.0880356428300573 1103 78-79 3.1639616793164755 1103 80-81 3.5499643158501044 1103 82-83 3.8379355225008283 1103 84-85 3.940296385758696 1103 86-87 4.114306285682897 1103 88-89 4.577121268555057 1103 90-91 4.556948058510134 1103 92-93 4.648470906876863 1103 94-95 4.919123726560706 1103 96-97 5.318545458572348 1103 98-99 5.434285060741253 1103 100-101 5.1484472066995455 1104 1 0.5483246374479194 1104 2 0.4864895857123628 1104 3 0.5679243518059529 1104 4 0.44599034460183873 1104 5 0.4752521701675221 1104 6 0.4093261791184233 1104 7 0.44893187996326134 1104 8 0.4724263029841538 1104 9 0.5351714377165564 1104 10-11 0.7527973805955384 1104 12-13 0.6619691181325322 1104 14-15 0.7881692456352383 1104 16-17 0.9214565939486832 1104 18-19 0.9555663085357011 1104 20-21 0.9717982018636704 1104 22-23 0.948905963329743 1104 24-25 0.9026150810832547 1104 26-27 0.9920697181383744 1104 28-29 0.9274206424348463 1104 30-31 0.9059760060012252 1104 32-33 1.0747137997454743 1104 34-35 0.9351538915934725 1104 36-37 1.2090692543082113 1104 38-39 1.1232366554997455 1104 40-41 1.4524246973108532 1104 42-43 1.3464817955707318 1104 44-45 1.4522170178065394 1104 46-47 1.4889296900503979 1104 48-49 1.699274643418768 1104 50-51 1.6294888801673082 1104 52-53 1.676272701153522 1104 54-55 1.9906753920614904 1104 56-57 2.1576434705304095 1104 58-59 2.16697557431673 1104 60-61 2.326415590355385 1104 62-63 2.3735093824080558 1104 64-65 2.5022022595931794 1104 66-67 2.6496909590615267 1104 68-69 2.822210651132881 1104 70-71 2.8412937804315668 1104 72-73 2.988125661195486 1104 74-75 2.970770194762661 1104 76-77 3.2124687149644764 1104 78-79 3.434337408257374 1104 80-81 3.658018058963375 1104 82-83 4.007762856171375 1104 84-85 4.0807560317979 1104 86-87 4.038339600083312 1104 88-89 4.475065304356171 1104 90-91 4.687380793431384 1104 92-93 4.990730498848929 1104 94-95 5.238374171390088 1104 96-97 5.425858368844448 1104 98-99 5.651639670530042 1104 100-101 5.431097306591882 1105 1 0.4443274369837731 1105 2 0.4080251453151078 1105 3 0.4175917591437397 1105 4 0.3539109739772215 1105 5 0.4528366719937793 1105 6 0.34241159747347183 1105 7 0.41302115300017306 1105 8 0.3242586807748964 1105 9 0.2725890432267235 1105 10-11 0.4468838044426633 1105 12-13 0.4459213226783447 1105 14-15 0.6404785080438486 1105 16-17 0.7758792517462112 1105 18-19 0.7700185872668541 1105 20-21 0.7270208790498813 1105 22-23 0.7552854884135485 1105 24-25 0.6330236501693065 1105 26-27 0.8268439021144687 1105 28-29 0.8715018939976105 1105 30-31 0.8309902962840141 1105 32-33 0.7837865480727473 1105 34-35 0.8339410056893328 1105 36-37 0.9144605184085961 1105 38-39 0.8445955003128915 1105 40-41 0.9239895468210264 1105 42-43 1.3660371682737207 1105 44-45 1.2236536242205247 1105 46-47 1.4053847363970249 1105 48-49 1.41957701220678 1105 50-51 1.2429294896338305 1105 52-53 1.378402913855595 1105 54-55 1.5048832138558978 1105 56-57 1.7247101173355048 1105 58-59 1.759830048697367 1105 60-61 2.0965634112867555 1105 62-63 2.1714372470703545 1105 64-65 2.203275700711007 1105 66-67 2.247969681938809 1105 68-69 2.386831989672782 1105 70-71 2.4496442709710173 1105 72-73 2.752042800608944 1105 74-75 2.7040855876582484 1105 76-77 3.042138740955945 1105 78-79 3.097784697359696 1105 80-81 3.391460394495077 1105 82-83 3.640357912413318 1105 84-85 3.9006307856710585 1105 86-87 4.226478488036268 1105 88-89 4.3673378524126 1105 90-91 4.5588801174877105 1105 92-93 4.504336414523554 1105 94-95 4.856554218904169 1105 96-97 5.118602511010742 1105 98-99 5.197318034835018 1105 100-101 4.801557149271678 1106 1 0.28019855287899986 1106 2 0.2569040832411922 1106 3 0.21001199595751885 1106 4 0.24738122873143453 1106 5 0.2706252618753737 1106 6 0.05106061074939561 1106 7 0.2860490683213044 1106 8 0.16800894988146808 1106 9 0.16353299729920678 1106 10-11 0.31387239432426384 1106 12-13 0.17749333559546443 1106 14-15 0.46431905343315094 1106 16-17 0.07980519361919391 1106 18-19 0.5063212783325142 1106 20-21 0.37679282020524596 1106 22-23 0.1742489616822951 1106 24-25 0.34879673951268586 1106 26-27 0.5500958576221748 1106 28-29 0.3090575452354969 1106 30-31 0.5330323486700905 1106 32-33 0.5462696481803917 1106 34-35 0.5319987021371304 1106 36-37 0.7088359758897624 1106 38-39 0.555876304044503 1106 40-41 0.6214127258665982 1106 42-43 0.27105159238566756 1106 44-45 0.6118102083611845 1106 46-47 0.8934885038889462 1106 48-49 0.9433089103050989 1106 50-51 0.31865966545012014 1106 52-53 0.8373195984626989 1106 54-55 0.5941780828907071 1106 56-57 0.8244028335177802 1106 58-59 0.34001017069234507 1106 60-61 1.378609769377892 1106 62-63 1.542694225900643 1106 64-65 1.7847406450956491 1106 66-67 1.87205493489898 1106 68-69 1.4343560657402428 1106 70-71 1.8536904855386531 1106 72-73 2.228433184534307 1106 74-75 2.5785035280959967 1106 76-77 2.7158554255630456 1106 78-79 3.0972922682244253 1106 80-81 3.6463457909787493 1106 82-83 3.68417405880729 1106 84-85 3.8430216001669315 1106 86-87 4.068593306837844 1106 88-89 3.9320669518026286 1106 90-91 4.532631534782293 1106 92-93 4.769441976059255 1106 94-95 4.695055115926056 1106 96-97 4.982534480870807 1106 98-99 5.1542342888716135 1106 100-101 4.5342393164765635 1107 1 0.3395336567264664 1107 2 0.4168817475244744 1107 3 0.41160709299531106 1107 4 0.27798333972905453 1107 5 0.33850513930131854 1107 6 0.41756902069150925 1107 7 0.28043490420827055 1107 8 0.2260845372154776 1107 9 0.33315478824234646 1107 10-11 0.3167197896255871 1107 12-13 0.42901488820015743 1107 14-15 0.598737303348031 1107 16-17 0.8104617819746593 1107 18-19 0.8000238319586614 1107 20-21 0.806025234233168 1107 22-23 0.656614913333641 1107 24-25 0.6863930622910317 1107 26-27 0.7245108388955828 1107 28-29 0.7761370140812645 1107 30-31 0.7439682696779215 1107 32-33 0.947386297822888 1107 34-35 1.0830042179696093 1107 36-37 0.9792294386068221 1107 38-39 0.8689646254609116 1107 40-41 0.8960302267178051 1107 42-43 0.9753535331415435 1107 44-45 1.0162799393792241 1107 46-47 0.9155228246243894 1107 48-49 1.1309490873565053 1107 50-51 1.321022620846783 1107 52-53 1.2946703645505409 1107 54-55 1.4258346031494682 1107 56-57 1.5270709983117854 1107 58-59 1.560264852340289 1107 60-61 1.5608184176584494 1107 62-63 1.7677595306333593 1107 64-65 1.7896086055995646 1107 66-67 1.9139699502207392 1107 68-69 2.1367498008440897 1107 70-71 2.1205318883306177 1107 72-73 2.580850617288821 1107 74-75 2.592578162076247 1107 76-77 2.614688111977756 1107 78-79 2.9876233543667468 1107 80-81 3.3035859680301627 1107 82-83 3.52340265942016 1107 84-85 3.665209819438296 1107 86-87 3.7390822411245317 1107 88-89 4.268924289221761 1107 90-91 4.382977057424444 1107 92-93 4.45659240166361 1107 94-95 4.487342483988709 1107 96-97 4.626045410390727 1107 98-99 4.866271878248529 1107 100-101 4.680842925601516 1108 1 0.30601040325966977 1108 2 0.33039471548217136 1108 3 0.2968171598105478 1108 4 0.3872379649115132 1108 5 0.2843444277145011 1108 6 0.2829115210439994 1108 7 0.2546785287648703 1108 8 0.30311123389338235 1108 9 0.28623197114892207 1108 10-11 0.31290000107699 1108 12-13 0.5015120049102535 1108 14-15 0.4500427210928635 1108 16-17 0.6460042005705375 1108 18-19 0.6096861244099685 1108 20-21 0.5842786858126559 1108 22-23 0.657625459530685 1108 24-25 0.5851566203468437 1108 26-27 0.645764311776432 1108 28-29 0.5495504945900151 1108 30-31 0.6250583003417205 1108 32-33 0.8579236700274677 1108 34-35 0.7096602711540072 1108 36-37 0.6342695409344472 1108 38-39 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0.010276273065173314 2311 14-15 0.03608198767131654 2311 16-17 -0.07091627996296523 2311 18-19 -0.004756957246733862 2311 20-21 0.047787035748946494 2311 22-23 -0.039457032824088856 2311 24-25 0.06170493146615286 2311 26-27 0.0976365911817183 2311 28-29 0.20577960049482868 2311 30-31 0.22937967457087893 2311 32-33 0.2724792458536882 2311 34-35 0.1523073788215683 2311 36-37 0.24196947080008613 2311 38-39 0.26336197216536306 2311 40-41 0.15091590893332096 2311 42-43 0.24643462680156603 2311 44-45 0.3045662594195093 2311 46-47 0.31231312822244206 2311 48-49 0.4278302223102628 2311 50-51 0.24000075447904834 2311 52-53 0.4445715616231425 2311 54-55 0.5692587744402289 2311 56-57 0.5903651537633721 2311 58-59 0.8025785985482159 2311 60-61 0.5731984913038879 2311 62-63 0.7121803464370764 2311 64-65 0.8213957428497309 2311 66-67 0.7136184831297108 2311 68-69 0.6437127954219335 2311 70-71 0.8753596404909878 2311 72-73 0.9986868604108672 2311 74-75 1.0250600638852987 2311 76-77 1.522873554501487 2311 78-79 1.7304790189111259 2311 80-81 1.5866522702161063 2311 82-83 1.7768476409691907 2311 84-85 2.03852161632463 2311 86-87 2.107060264343094 2311 88-89 2.2818326651650445 2311 90-91 2.717492675515853 2311 92-93 2.9115517837826346 2311 94-95 2.9414044453815436 2311 96-97 2.991869623866446 2311 98-99 3.305104671809083 2311 100-101 3.0387676086077615 2312 1 -0.35946786595619074 2312 2 -0.2697707618641374 2312 3 -0.24481105236962009 2312 4 -0.3210978295089575 2312 5 -0.13244760057816052 2312 6 -0.18730717115890627 2312 7 -0.14985774704424415 2312 8 -0.28629960030441026 2312 9 -0.07212846490649838 2312 10-11 -0.21019414842667317 2312 12-13 -0.25234644135620954 2312 14-15 -0.2542678114986927 2312 16-17 -0.09574015954065374 2312 18-19 -0.03932816404666539 2312 20-21 -0.11645488735361909 2312 22-23 -0.1953182006473213 2312 24-25 -0.27374117870292736 2312 26-27 -0.17964416716096565 2312 28-29 -0.19301940643736515 2312 30-31 -0.09431362406844812 2312 32-33 0.012952547808644965 2312 34-35 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100-101 3.280333244605435 2313 1 -0.4668983742774486 2313 2 -0.5810801313899105 2313 3 -0.5444265627696687 2313 4 -0.4470572052102213 2313 5 -0.2757092257240643 2313 6 -0.3939143385564847 2313 7 -0.35210510419467766 2313 8 -0.3601295620511493 2313 9 -0.3936815402144447 2313 10-11 -0.41373977226442094 2313 12-13 -0.3643649704410379 2313 14-15 -0.3542616360068749 2313 16-17 -0.30045760469619864 2313 18-19 -0.2045376828279899 2313 20-21 -0.2478216237192612 2313 22-23 -0.4165533468499163 2313 24-25 -0.2984141168279777 2313 26-27 -0.30680471951087185 2313 28-29 -0.3011893429254755 2313 30-31 -0.26176621629637253 2313 32-33 -0.28588308930205386 2313 34-35 -0.06841159266867436 2313 36-37 0.10312965862256362 2313 38-39 0.13500455236769682 2313 40-41 0.12360969981761372 2313 42-43 0.12269632799633712 2313 44-45 0.1630522610830596 2313 46-47 0.02645284642521517 2313 48-49 0.1434270821971566 2313 50-51 0.3709635182032045 2313 52-53 0.45101131582347875 2313 54-55 0.4057028996119669 2313 56-57 0.41427636338831064 2313 58-59 0.4534969902212751 2313 60-61 0.6078767168119903 2313 62-63 0.6471538109887724 2313 64-65 0.7645724342081124 2313 66-67 0.7503396330739918 2313 68-69 1.1223472059679729 2313 70-71 1.1833191862093742 2313 72-73 1.1341265241069216 2313 74-75 1.085884841463642 2313 76-77 1.310465439601419 2313 78-79 1.378329111150645 2313 80-81 1.8556053760668796 2313 82-83 1.841569660117539 2313 84-85 2.02321040066607 2313 86-87 2.210026468591078 2313 88-89 2.4135518995486898 2313 90-91 2.4288933348134876 2313 92-93 2.5008914223234875 2313 94-95 2.792893830635151 2313 96-97 2.930967611427665 2313 98-99 3.064058528459821 2313 100-101 3.103185565111721 2314 1 -0.41791335847834077 2314 2 -0.43305935166185705 2314 3 -0.6407514389455287 2314 4 -0.34469116277364265 2314 5 -0.42558083230745325 2314 6 -0.41933938925060943 2314 7 -0.32019211358081634 2314 8 -0.34287562998926546 2314 9 -0.34242009596025724 2314 10-11 -0.455541086746841 2314 12-13 -0.5779275323639084 2314 14-15 -0.6281947046462548 2314 16-17 -0.5565515930844 2314 18-19 -0.43133375398512186 2314 20-21 -0.4380082878279907 2314 22-23 -0.5544775387793877 2314 24-25 -0.4785683574402242 2314 26-27 -0.4759233482873313 2314 28-29 -0.3407655387903574 2314 30-31 -0.3583995996216913 2314 32-33 -0.3344446639156331 2314 34-35 -0.4470236431998984 2314 36-37 -0.3936751967787515 2314 38-39 -0.09080550574312696 2314 40-41 -0.14743824366249214 2314 42-43 0.09646544280118263 2314 44-45 -0.24098905294999184 2314 46-47 -0.1138584951876922 2314 48-49 -0.11755646365612193 2314 50-51 -0.015633963358745007 2314 52-53 0.04285311646823686 2314 54-55 0.14594798132098674 2314 56-57 0.2718706082176823 2314 58-59 0.36756511067387265 2314 60-61 0.33597929846376573 2314 62-63 0.4066473191601041 2314 64-65 0.6058069424179031 2314 66-67 0.7638089515194793 2314 68-69 0.9308044498584316 2314 70-71 1.1128398853539814 2314 72-73 1.236928106048623 2314 74-75 1.2517027894071653 2314 76-77 1.3513257856738505 2314 78-79 1.4964627206713281 2314 80-81 1.7635291704801368 2314 82-83 1.8147461733040586 2314 84-85 2.055774324081984 2314 86-87 2.255097461962496 2314 88-89 2.6270346341664315 2314 90-91 2.6455523104055594 2314 92-93 2.9178789105006224 2314 94-95 2.9659526228512654 2314 96-97 2.914708442089644 2314 98-99 2.9110390903489893 2314 100-101 2.8946116156455357 2315 1 -0.668598802095449 2315 2 -0.6039548286914815 2315 3 -0.3785239824013651 2315 4 -0.3718774427443634 2315 5 -0.47867825480631865 2315 6 -0.4566102037982489 2315 7 -0.423215975261499 2315 8 -0.5437349635540656 2315 9 -0.48858506702300986 2315 10-11 -0.5110062350715907 2315 12-13 -0.5099714074162591 2315 14-15 -0.7159801951391316 2315 16-17 -0.4548397117009131 2315 18-19 -0.43719468199210354 2315 20-21 -0.36252560480227203 2315 22-23 -0.412437302219125 2315 24-25 -0.4576028998020121 2315 26-27 -0.5525633548815776 2315 28-29 -0.5331004349268085 2315 30-31 -0.47302271595269474 2315 32-33 -0.5055813887898424 2315 34-35 -0.5553751481784701 2315 36-37 -0.37199432167561497 2315 38-39 -0.3862552258623211 2315 40-41 -0.38510858462333175 2315 42-43 -0.33267825432307774 2315 44-45 -0.24259327826340638 2315 46-47 -0.2659131191949058 2315 48-49 -0.4888452225473259 2315 50-51 -0.30645605441763024 2315 52-53 0.012977308111032926 2315 54-55 -0.11076054049372885 2315 56-57 0.27077926874471103 2315 58-59 0.2875599152069199 2315 60-61 0.3832363999760311 2315 62-63 0.41650330314740813 2315 64-65 0.4214196952910214 2315 66-67 0.655996143194745 2315 68-69 0.6930631291607376 2315 70-71 0.612607588033395 2315 72-73 0.7418987691450702 2315 74-75 0.6360091487752833 2315 76-77 0.8958294562212963 2315 78-79 1.2026515708995085 2315 80-81 1.1813119466745725 2315 82-83 1.5361675664718497 2315 84-85 1.7733380414052853 2315 86-87 1.973521470348512 2315 88-89 2.1704498793649982 2315 90-91 2.233088643288454 2315 92-93 2.462254359587959 2315 94-95 2.4876634718022252 2315 96-97 2.475258736957368 2315 98-99 2.654406335760701 2315 100-101 2.6184685921002444 2316 1 -0.8725557790544265 2316 2 -0.7427343053106839 2316 3 -0.7268301878968906 2316 4 -0.5826494447792143 2316 5 -0.5620743181526251 2316 6 -0.5185772178398125 2316 7 -0.4285892269034548 2316 8 -0.5643512927690253 2316 9 -0.6827189236838436 2316 10-11 -0.5595110867770074 2316 12-13 -0.4981841259071018 2316 14-15 -1.0174559037919195 2316 16-17 -0.7489780075007317 2316 18-19 -0.6586616428060736 2316 20-21 -0.4750355823456971 2316 22-23 -0.677104343652907 2316 24-25 -0.8388068069175532 2316 26-27 -0.5806452218614098 2316 28-29 -0.756526402501315 2316 30-31 -0.6602061643179269 2316 32-33 -0.5461548035228247 2316 34-35 -0.6002062441997822 2316 36-37 -0.3175016815997438 2316 38-39 -0.34803771057674027 2316 40-41 -0.3607264559657253 2316 42-43 -0.17288345194470622 2316 44-45 -0.2283330176459728 2316 46-47 -0.12136581248996237 2316 48-49 -0.07728738771947974 2316 50-51 -9.888024297950437E-4 2316 52-53 0.025835137426767574 2316 54-55 -0.20249660990755558 2316 56-57 0.04699869601583373 2316 58-59 0.13696086286842046 2316 60-61 0.16898861523261388 2316 62-63 0.35493339217844166 2316 64-65 0.3564784581303364 2316 66-67 0.5878466287860569 2316 68-69 0.899532111781614 2316 70-71 0.6099938608691851 2316 72-73 0.8338306024842232 2316 74-75 1.135492546605871 2316 76-77 1.2260851969116224 2316 78-79 1.2590033897052173 2316 80-81 1.6788772577698552 2316 82-83 2.0781925375753723 2316 84-85 2.059056068980123 2316 86-87 2.3810201228278878 2316 88-89 2.2735197438387154 2316 90-91 2.491256764211439 2316 92-93 2.647313122427274 2316 94-95 2.7282820563522527 2316 96-97 2.8493578126486874 2316 98-99 2.7865448814987737 2316 100-101 2.5757887954001877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5348.0 3 1794.0 4 1887.0 5 2221.0 6 2617.0 7 3386.0 8 4257.0 9 5664.0 10 7266.0 11 9146.0 12 11771.0 13 14159.0 14 16528.0 15 20081.0 16 23723.0 17 27536.0 18 26194.0 19 23535.0 20 25151.0 21 27263.0 22 30165.0 23 34352.0 24 38420.0 25 43154.0 26 48714.0 27 55139.0 28 62232.0 29 70225.0 30 80873.0 31 91672.0 32 107173.0 33 124577.0 34 148651.0 35 179185.0 36 205268.0 37 205226.0 38 108841.0 39 8129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.023886624513594 18.742433889003472 14.534950883247028 22.69872860323591 2 15.592143403508071 20.352690393997708 39.95083314306997 24.104333059424242 3 18.969341511175088 25.177746000780747 29.515148262839958 26.337764225204207 4 12.793339296048826 16.38212237596675 36.49492292313358 34.32961540485084 5 13.62832240248533 38.018408446079626 33.41698098116639 14.936288170268655 6 32.188628016374984 35.82911505635478 17.256243225324393 14.726013701945845 7 29.063844538887107 33.142904937829336 20.15479408746134 17.63845643582221 8 27.248762699167557 34.135930542813135 19.49180182985851 19.1235049281608 9 27.559579143630202 16.16516492344503 19.732153613942828 36.54310231898194 10-11 25.23168027346334 26.67743146254017 26.67808902866699 21.4127992353295 12-13 26.476721498098023 24.147819184109565 27.208762928628655 22.166696389163757 14-15 23.22098373211581 25.249399597215277 25.718938114918053 25.81067855575086 16-17 22.03653411897259 28.583571744549417 26.604771801147425 22.77512233533057 18-19 22.267018856054293 26.54681426498525 29.45753173031579 21.728635148644663 20-21 22.973805105354323 25.766375261560963 29.578464342878636 21.681355290206078 22-23 22.72839297376617 25.70801670628258 29.599047639839103 21.964542680112142 24-25 22.351852957758187 26.03472275020724 29.70975278725666 21.90367150477791 26-27 21.813233932404565 26.370612189065195 29.482169914558693 22.333983963971548 28-29 21.964465097046684 26.466787331126092 29.530131963710176 22.03861560811705 30-31 21.963539297957748 26.28614236071564 29.205176475910882 22.54514186541573 32-33 21.698052413473842 26.35553153238459 29.224240080907233 22.72217597323433 34-35 22.547135891137483 26.410907293737157 28.989648160554115 22.05230865457124 36-37 22.68870408352749 25.90686167495801 28.96562771077429 22.438806530740212 38-39 22.177507373040893 26.30769894323692 29.008359531933536 22.50643415178865 40-41 22.398571207656694 26.138920652687325 29.258236700670004 22.20427143898598 42-43 22.240056175638287 26.12056722666975 28.88655992594073 22.752816671751226 44-45 22.036630988339283 26.432107654544872 29.023778480556615 22.50748287655923 46-47 22.644714473866923 26.694331766596957 28.29038308597974 22.37057067355638 48-49 22.314457324047115 26.926314687699954 27.990646107107438 22.768581881145494 50-51 22.58362261815009 26.94157862336629 27.929753292929444 22.54504546555418 52-53 22.782101295214506 26.66905961194842 28.03109251100163 22.51774658183544 54-55 22.337620741124088 27.070204724773877 27.98488152301852 22.607293011083517 56-57 22.248287838737603 27.262598361836922 27.752921797540548 22.736192001884927 58-59 22.278172841921574 26.549151957605506 28.032023073779612 23.14065212669331 60-61 21.891611941625104 27.015206430842127 28.214717632985597 22.878463994547175 62-63 22.132613871982688 26.909011542673973 28.449910527512955 22.508464057830388 64-65 22.003393880233844 26.83568923038651 28.21151768191616 22.949399207463486 66-67 21.81126034435109 27.01581231900426 28.063559563819496 23.109367772825152 68-69 22.40514467762273 26.58520839441827 28.26950915978242 22.740137768176577 70-71 22.322972399039077 26.55514603881307 27.921137857536387 23.20074370461147 72-73 22.167981000173302 26.907576236120867 27.749061750751665 23.175381012954162 74-75 22.176453030842925 27.264634840657383 27.691520792709305 22.867391335790387 76-77 22.27927225488172 26.865007935140905 27.89466971537366 22.96105009460371 78-79 22.34498264436731 27.037288313873987 27.758546334280005 22.859182707478702 80-81 22.47033467306343 27.061452273035663 27.41777279425764 23.05044025964327 82-83 22.59235367117562 26.947104113177804 27.547906252144923 22.91263596350165 84-85 22.31441012437741 27.011163437212144 27.655073377649092 23.019353060761357 86-87 22.558385058053084 26.960558613825718 27.412282720021246 23.068773608099953 88-89 22.56604724336556 26.94733315204213 27.264768088399975 23.221851516192338 90-91 22.397987189532376 26.912134437054565 27.447740651286008 23.242137722127055 92-93 22.62025669980387 26.577129070992438 27.503317489679358 23.299296739524333 94-95 22.50780653521337 26.28660879060707 27.767305943855014 23.438278730324548 96-97 22.84081560947694 25.98529668567918 27.639590491078287 23.534297213765594 98-99 22.60437524226985 26.22284145124702 27.360306613039892 23.812476693443237 100-101 23.101285231199213 25.62800023058196 27.775439494214787 23.495275044004043 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1064.0 1 707.0 2 490.5 3 657.0 4 577.5 5 579.5 6 1037.5 7 2198.0 8 3553.0 9 3851.0 10 3065.5 11 2449.0 12 2412.5 13 2474.0 14 2563.5 15 2640.0 16 2733.0 17 2872.5 18 3085.0 19 3377.5 20 3698.0 21 4265.0 22 4953.0 23 5714.5 24 6757.5 25 8121.5 26 9876.0 27 11846.5 28 13713.5 29 16061.5 30 18936.5 31 23505.5 32 30227.0 33 33867.0 34 34998.0 35 38015.5 36 43795.5 37 49289.0 38 52714.5 39 54969.5 40 56374.5 41 57934.0 42 58541.5 43 59979.5 44 62008.0 45 64629.5 46 65448.0 47 65339.0 48 66199.5 49 65406.0 50 64401.5 51 63404.5 52 62312.0 53 63066.5 54 64707.0 55 62259.0 56 57307.5 57 53011.0 58 50586.5 59 46501.0 60 38741.0 61 32419.0 62 27599.5 63 21375.5 64 15389.0 65 11583.5 66 8715.0 67 6753.5 68 5455.5 69 4325.0 70 3388.0 71 2449.5 72 1773.5 73 1378.5 74 1059.5 75 791.0 76 570.0 77 398.5 78 267.0 79 163.5 80 112.0 81 73.5 82 38.0 83 21.5 84 18.0 85 15.0 86 13.0 87 9.0 88 5.5 89 4.0 90 3.5 91 4.5 92 4.0 93 3.5 94 2.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3015477593486905 2 0.03413053641738754 3 0.04086198273699556 4 0.04328109625810469 5 0.05737506198978398 6 0.05590255810736973 7 0.028713825707077957 8 0.0321321382912539 9 0.03849545864025836 10-11 0.03016003487730624 12-13 0.03186919116939422 14-15 0.02668913286875836 16-17 0.02082541205128731 18-19 0.024322608772021165 20-21 0.035524156163243885 22-23 0.029528961784842992 24-25 0.05014401613864255 26-27 0.042229307770665934 28-29 0.03468272537329288 30-31 0.03441977825143319 32-33 0.030528160847909807 34-35 0.029266014662983304 36-37 0.028608646858334082 38-39 0.0178804042864588 40-41 0.01772263601334299 42-43 0.018038172559574617 44-45 0.009571275235692652 46-47 0.017407099467111366 48-49 0.014856512385072386 50-51 0.007283635275513365 52-53 0.005285237149379734 54-55 0.007415108836443209 56-57 0.006573678046492207 58-59 0.007572877109559022 60-61 0.006494793909934299 62-63 0.005285237149379734 64-65 0.008388013187324055 66-67 0.0032868390232461036 68-69 0.00738881412425724 70-71 0.012121862317731628 72-73 0.01275293541019488 74-75 0.010281232464713812 76-77 0.00896649685541537 78-79 0.0064684991977483315 80-81 0.00762546653393096 82-83 0.012174451742103567 84-85 0.004864521754404233 86-87 0.0016039774433440983 88-89 0.018195940832690428 90-91 0.02626841747378286 92-93 0.012411104151777286 94-95 0.02058875964161359 96-97 0.01677602637464811 98-99 0.012305925303033411 100-101 0.10420594439299447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1901523.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.74595832355393 #Duplication Level Percentage of deduplicated Percentage of total 1 73.21280846548999 33.49190084812641 2 13.60470315160569 12.447203667553536 3 5.078533599562683 6.969671591710886 4 2.5248770414346637 4.620116796382731 5 1.422905268665063 3.254608255935864 6 0.8720868094703109 2.393666810432998 7 0.5915819231399692 1.8943737400650238 8 0.4030524089749459 1.4750414962540712 9 0.29889518885668215 1.2305922167293706 >10 1.7276162181979016 14.877887029303574 >50 0.16186116732622904 5.157187210654138 >100 0.09307207132103444 7.638686276690645 >500 0.005221751709725564 1.6581043091728074 >1k 0.002436817464538597 1.7151686622385254 >5k 3.4811678064837096E-4 1.1757910887494456 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9865 0.5187946714291649 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6932 0.3645498897462718 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5353 0.28151118866298225 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2439 0.12826560604315593 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2314 0.12169192799666373 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2148 0.11296208355092209 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.6294712185968825E-5 0.0 12-13 0.0 0.0 0.0 5.258942437193765E-5 0.0 14-15 0.0 0.0 0.0 1.051788487438753E-4 0.0 16-17 0.0 0.0 0.0 1.5776827311581295E-4 0.0 18-19 0.0 0.0 0.0 1.5776827311581295E-4 0.0 20-21 0.0 0.0 0.0 2.103576974877506E-4 0.0 22-23 0.0 0.0 0.0 5.784836680913141E-4 0.0 24-25 0.0 0.0 0.0 0.0010254937752527842 0.0 26-27 0.0 0.0 0.0 0.0021824611114354127 0.0 28-29 5.258942437193765E-5 0.0 0.0 0.005758541968727172 0.0 30-31 5.258942437193765E-5 0.0 0.0 0.020036570685708247 0.0 32-33 5.258942437193765E-5 0.0 0.0 0.04538467323298219 0.0 34-35 5.258942437193765E-5 0.0 0.0 0.08224985971771048 0.0 36-37 5.258942437193765E-5 0.0 0.0 0.1476973983485869 0.0 38-39 1.051788487438753E-4 0.0 0.0 0.2491949873864266 0.0 40-41 1.051788487438753E-4 0.0 0.0 0.36570685708245443 0.0 42-43 1.051788487438753E-4 0.0 0.0 0.48187689552006474 0.0 44-45 1.051788487438753E-4 0.0 0.0 0.62071297586198 0.0 46-47 2.8924183404565706E-4 0.0 0.0 0.7812684884695058 0.0 48-49 3.155365462316259E-4 0.0 0.0 0.9579426596470303 0.0 50-51 3.155365462316259E-4 0.0 0.0 1.1550215274808666 0.0 52-53 3.155365462316259E-4 0.0 0.0 1.345395243707281 0.0 54-55 3.155365462316259E-4 0.0 0.0 1.5464972025055705 0.0 56-57 3.155365462316259E-4 0.0 0.0 1.773788694641085 0.0 58-59 3.155365462316259E-4 0.0 0.0 2.0252713219876908 0.0 60-61 3.155365462316259E-4 0.0 0.0 2.3026542408374757 0.0 62-63 3.155365462316259E-4 0.0 0.0 2.574383796567278 0.0 64-65 3.155365462316259E-4 0.0 0.0 2.860338791589689 0.0 66-67 3.681259706035636E-4 0.0 0.0 3.1807924490000907 0.0 68-69 3.681259706035636E-4 0.0 0.0 3.538216471743965 0.0 70-71 3.681259706035636E-4 0.0 0.0 3.9189376094846082 0.0 72-73 3.681259706035636E-4 0.0 0.0 4.314094544215347 0.0 74-75 3.681259706035636E-4 0.0 0.0 4.7025463273386645 0.0 76-77 3.681259706035636E-4 0.0 0.0 5.115557371643677 0.0 78-79 3.681259706035636E-4 0.0 0.0 5.575583361337202 0.0 80-81 4.733048193474389E-4 0.0 0.0 6.073342263017592 0.0 82-83 4.733048193474389E-4 0.0 0.0 6.590191125744996 0.0 84-85 4.733048193474389E-4 0.0 0.0 7.10383203358571 5.258942437193765E-5 86-87 4.733048193474389E-4 0.0 0.0 7.63335494758675 5.258942437193765E-5 88-89 4.733048193474389E-4 0.0 0.0 8.182362243317593 5.258942437193765E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3815 0.0 59.3517 1 GTATCAA 7765 0.0 47.269756 1 ATCAACG 10665 0.0 34.35847 3 TATCAAC 10710 0.0 34.302864 2 TCAACGC 10840 0.0 33.89148 4 CAACGCA 11080 0.0 33.286053 5 AACGCAG 11555 0.0 31.99495 6 ACGCAGA 13175 0.0 27.876104 7 CGCAGAG 13285 0.0 27.573761 8 GTACATG 10355 0.0 27.252457 1 TACATGG 10355 0.0 27.125498 2 GTATAGG 565 0.0 26.154552 1 GTGTAAG 835 0.0 25.689764 1 GCAGAGT 14235 0.0 25.065376 9 ACATGGG 10845 0.0 24.497547 3 TGGTATC 1635 0.0 23.836168 2 TAAGGTA 525 0.0 23.537178 4 GAGTACT 7785 0.0 22.486067 12-13 AGAGTAC 13015 0.0 22.33817 10-11 AGTACTT 8245 0.0 22.26863 12-13 >>END_MODULE