FastQCFastQC Report
Thu 26 May 2016
SRR938545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901523
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96220.5060154413067841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74640.3925274635121426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57140.30049597086125174No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24080.12663533388762588No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22660.11916763562681072No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20990.11038520175669714No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20020.10528402759261918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39800.061.5498961
GTATCAA80550.047.1238251
ATCAACG110500.034.073333
TATCAAC111900.033.709032
TCAACGC113250.033.2879184
CAACGCA114650.032.8814355
AACGCAG119350.031.5981246
TGGTATC17950.028.0895392
TAAGGTG10700.027.5460625
ACGCAGA138000.027.2245457
GTACATG107150.026.9463351
CGCAGAG139050.026.8481738
TACATGG108700.026.4308152
AGGTAAG9600.025.2698172
ACATGGG108000.025.2661913
TAAGGTA4400.024.8500234
GCAGAGT152500.023.9819269
GTAAGGT11550.023.8724864
GAGTACT83250.022.84975212-13
ACCGTAT1252.257727E-422.7984038
Produced by FastQC (version 0.11.5)