FastQCFastQC Report
Thu 26 May 2016
SRR938544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898984
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93550.49263184945212807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66590.3506611956709483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49930.26293007208064945No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22530.11864238982529605No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22200.11690461846966588No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20610.10853172011981144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.061.5594441
GTATCAA79700.046.4400671
ATCAACG104750.035.118813
TATCAAC105500.034.5988432
TCAACGC106750.034.37184
CAACGCA108000.034.0179865
AACGCAG110750.033.0787476
TGGTATC14450.030.5891862
ACGCAGA127700.028.6813037
CGCAGAG128400.028.4509478
GTACATG101200.027.3832551
TAAGGTG10000.026.6158815
TACATGG101350.026.5427252
GCAGAGT138050.025.9797239
GTGTAAG7350.025.9574531
AGGTAAG8050.025.387822
ACATGGG103750.024.9667053
ACGATAG1650.024.4891594-95
GAGTACT75400.024.38071412-13
GGTAAGG9100.024.0253263
Produced by FastQC (version 0.11.5)