FastQCFastQC Report
Thu 26 May 2016
SRR938544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898984
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92760.4884717301462256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73430.3866804564967372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.28936526058144774No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23380.12311846755949497No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22810.12011686249067922No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20990.1105327901656886No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20310.10695192797832948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.058.1082341
GTATCAA82450.044.0316121
ATCAACG109950.032.6159973
TCAACGC111650.032.076844
TATCAAC112050.032.0513272
CAACGCA113900.031.4431845
AACGCAG117800.030.4021996
TAAGGTG10800.027.2675975
GTACATG105900.027.1020981
TACATGG105050.026.8612652
ACGCAGA134600.026.5017037
AGGTAAG9400.026.2791652
CGCAGAG135700.026.1468688
TGGTATC20300.025.7414362
ACATGGG105400.025.5067273
GTAAGGT10900.025.2743764
GGTAAGG10850.024.0775283
GCAGAGT148250.023.4207949
GAGTACT81050.022.82618512-13
TGTAAGG7500.022.1688372
Produced by FastQC (version 0.11.5)