FastQCFastQC Report
Thu 26 May 2016
SRR938543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194297
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48530.40634783475132236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40280.33726954015625926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26490.22180412409978423No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.17123043932958049No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16140.13514226360779605No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14650.12266630494759678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12930.10826452716535334No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA12190.10206841346834164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32000.044.126061
TACCTGG12800.042.714622
GTACCTG13800.039.6193541
GTACGAC853.6149868E-739.1532443
GTATCAA50550.035.1754041
ACCTGGG15200.032.5295753
TACGACG1102.7142505E-630.2547824
GAACCGA804.4896608E-429.692116
GTACATG54050.029.3791621
TAGGACC15000.029.1598454
TACATGG54450.028.3774992
TATCAAC64600.027.5250262
ATCAACG64100.027.4430473
TAACGCA1056.863421E-527.1675574
TCAACGC64950.027.0107024
TGTACGA1257.3391693E-626.6242082
ACATGGG53400.026.6206463
CAACGCA66300.026.532425
AACGCAG67550.026.2327926
CGACGTG1501.0587355E-625.3372656
Produced by FastQC (version 0.11.5)