##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938543_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1194297 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.068939300693213 31.0 31.0 34.0 28.0 34.0 2 31.1207438350762 31.0 31.0 34.0 27.0 34.0 3 31.146384023404565 31.0 31.0 34.0 27.0 34.0 4 34.802806169654616 37.0 35.0 37.0 32.0 37.0 5 34.80133333668258 37.0 35.0 37.0 32.0 37.0 6 34.82456038991976 37.0 35.0 37.0 32.0 37.0 7 34.7507964936695 37.0 35.0 37.0 32.0 37.0 8 34.71931604952537 37.0 35.0 37.0 31.0 37.0 9 36.20071054352477 39.0 35.0 39.0 32.0 39.0 10-11 36.00070041204156 38.5 35.0 39.0 30.0 39.0 12-13 35.878781408644585 38.0 35.0 39.0 30.0 39.0 14-15 36.80945192025099 39.0 36.0 41.0 30.5 41.0 16-17 36.657014544958244 39.0 36.0 40.5 30.0 41.0 18-19 36.85617271080812 39.0 36.0 41.0 30.0 41.0 20-21 36.87657383381186 39.0 36.0 41.0 30.0 41.0 22-23 36.759081283801265 39.0 36.0 41.0 30.0 41.0 24-25 36.6442463641791 39.0 36.0 41.0 30.0 41.0 26-27 36.45128305605725 39.0 35.5 41.0 29.5 41.0 28-29 36.23697204296754 39.0 35.0 40.5 29.0 41.0 30-31 35.94830264163772 38.5 35.0 40.0 27.5 41.0 32-33 35.66133424098026 38.0 35.0 40.0 27.0 41.0 34-35 35.44187249905174 38.0 34.5 40.0 27.0 41.0 36-37 35.1243149735786 38.0 34.0 40.0 25.5 41.0 38-39 34.927829928401394 38.0 34.0 40.0 25.0 41.0 40-41 34.78896999657539 38.0 34.0 40.0 24.5 41.0 42-43 34.55438555066286 38.0 33.5 40.0 24.0 41.0 44-45 34.21031703169312 38.0 33.0 40.0 23.5 41.0 46-47 33.74917252576202 38.0 33.0 40.0 20.0 41.0 48-49 33.392845330767805 38.0 32.5 40.0 17.5 41.0 50-51 32.74195865852464 37.5 31.5 39.5 15.0 40.5 52-53 32.81488231151883 37.0 32.0 39.5 15.0 40.0 54-55 32.962447364432805 38.0 32.0 40.0 14.0 41.0 56-57 32.64738126278472 37.0 31.5 40.0 10.5 41.0 58-59 32.29531222133188 37.0 31.0 40.0 8.0 41.0 60-61 31.892563575057125 36.5 31.0 39.5 7.5 41.0 62-63 31.362639276494875 36.0 29.5 39.0 2.0 40.5 64-65 30.850942855922774 35.5 29.0 39.0 2.0 40.0 66-67 30.283147742981853 35.0 28.0 38.5 2.0 40.0 68-69 29.69349332703674 34.5 28.0 38.0 2.0 40.0 70-71 29.077106867052333 34.0 26.5 37.5 2.0 40.0 72-73 28.451516247633545 34.0 26.0 37.0 2.0 39.0 74-75 27.795012882055303 33.5 25.5 36.0 2.0 39.0 76-77 27.029390930396712 32.5 23.0 36.0 2.0 38.5 78-79 26.247366023694276 32.0 21.5 35.0 2.0 38.0 80-81 25.53809981939166 31.5 19.0 35.0 2.0 37.0 82-83 24.81225482438623 31.0 15.5 35.0 2.0 36.5 84-85 24.080572922815684 31.0 7.5 34.0 2.0 36.0 86-87 23.25876477961512 30.0 4.5 34.0 2.0 35.5 88-89 22.38276576094556 29.5 2.0 34.0 2.0 35.0 90-91 21.42646259682474 29.0 2.0 34.0 2.0 35.0 92-93 20.519127989101538 28.0 2.0 34.0 2.0 35.0 94-95 19.471239566037596 26.0 2.0 33.0 2.0 35.0 96-97 18.063040432991123 24.5 2.0 32.5 2.0 34.5 98-99 16.510044402690454 10.5 2.0 32.0 2.0 34.0 100-101 13.122428089495326 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31890434862910055 1101 2 0.21378141604450462 1101 3 0.35784503041514526 1101 4 0.07352649970341929 1101 5 0.17033405516478695 1101 6 0.17640708937106808 1101 7 0.1609820000601303 1101 8 0.2244545163212237 1101 9 0.29520371248884913 1101 10-11 0.6459056095375004 1101 12-13 0.3943243479602927 1101 14-15 0.5891792292514779 1101 16-17 0.7137884299553292 1101 18-19 0.6997167298327369 1101 20-21 0.6041644796403887 1101 22-23 0.684186205658925 1101 24-25 0.5838203862114142 1101 26-27 0.5800446423920533 1101 28-29 0.5525864016031434 1101 30-31 0.5488943988834549 1101 32-33 0.7274777233440517 1101 34-35 0.6509741696365765 1101 36-37 0.6814182153763895 1101 38-39 0.6581090444869631 1101 40-41 0.7713547305604891 1101 42-43 0.9976973345101072 1101 44-45 1.1042327029275825 1101 46-47 1.0216451539346423 1101 48-49 0.9263591425309698 1101 50-51 0.8706815329504849 1101 52-53 0.8837359287460274 1101 54-55 0.9575407135095517 1101 56-57 0.8026181564362247 1101 58-59 0.9337686246441663 1101 60-61 1.0202178869784824 1101 62-63 1.124007079070985 1101 64-65 1.066307288702422 1101 66-67 1.2069794093839938 1101 68-69 1.176394482158102 1101 70-71 1.1978365944223874 1101 72-73 1.3502944334473774 1101 74-75 1.2068091763901698 1101 76-77 1.4105391089795027 1101 78-79 1.4714162354889098 1101 80-81 1.5718040910022992 1101 82-83 1.445499703002458 1101 84-85 1.5645022825846802 1101 86-87 1.9061683378511205 1101 88-89 1.752078720850065 1101 90-91 1.254329159955521 1101 92-93 1.3068640022563827 1101 94-95 1.070876684812582 1101 96-97 0.8807883112238777 1101 98-99 1.1877048225533677 1101 100-101 1.037100850095321 1102 1 0.6098709041974253 1102 2 0.45933556690838984 1102 3 0.43973983177897225 1102 4 0.3680788500079828 1102 5 0.36752211783957733 1102 6 0.5266924971786011 1102 7 0.47958905672027896 1102 8 0.5442185281206307 1102 9 0.8988508028616025 1102 10-11 0.9522401304737897 1102 12-13 0.9397386681533746 1102 14-15 0.9995017975386489 1102 16-17 1.0966716234579934 1102 18-19 0.9685249128002127 1102 20-21 0.8672012953320731 1102 22-23 1.0388695753865491 1102 24-25 1.0661500034967801 1102 26-27 1.1014617208887785 1102 28-29 0.7196285040434773 1102 30-31 0.9916844996381613 1102 32-33 0.882339233515296 1102 34-35 0.9928410048662357 1102 36-37 0.9593115609688496 1102 38-39 1.0044772780233373 1102 40-41 1.2773655339164236 1102 42-43 1.2684669778461242 1102 44-45 1.4193957110109423 1102 46-47 1.4535591101848837 1102 48-49 1.5311593570657038 1102 50-51 1.6443991133052336 1102 52-53 1.3306041048177448 1102 54-55 1.1542422420694933 1102 56-57 1.5443834707679116 1102 58-59 1.7028909477487915 1102 60-61 1.5358521052675762 1102 62-63 1.4089685777492988 1102 64-65 1.5462597845841195 1102 66-67 1.187680861324381 1102 68-69 1.2474744346539843 1102 70-71 1.5345354412982246 1102 72-73 1.6540679460704446 1102 74-75 1.799633755940409 1102 76-77 1.6841762388113253 1102 78-79 1.6101493313016526 1102 80-81 1.8384401098507048 1102 82-83 1.855393201834012 1102 84-85 1.5839291683832464 1102 86-87 2.0795258063413335 1102 88-89 1.8894556507048677 1102 90-91 2.078077388128527 1102 92-93 2.4550002318085404 1102 94-95 2.3231216375000123 1102 96-97 1.5064252111970653 1102 98-99 2.1465179857913093 1102 100-101 1.795304887945374 1103 1 0.44760088255240404 1103 2 0.28550124151156453 1103 3 0.3969751312017742 1103 4 0.29212286155199507 1103 5 0.2993808840733436 1103 6 0.5953568189679217 1103 7 0.20937801775924214 1103 8 0.3131421392317435 1103 9 0.39047012104342116 1103 10-11 0.5280743656830182 1103 12-13 0.7049138575473108 1103 14-15 0.7281825785631781 1103 16-17 0.4784086364450033 1103 18-19 0.6707728384856395 1103 20-21 0.6460139757072554 1103 22-23 0.7731273315192979 1103 24-25 0.8144973159065998 1103 26-27 0.6823261689407261 1103 28-29 0.6696363954576228 1103 30-31 0.6039567759729323 1103 32-33 0.8025829654777752 1103 34-35 0.7539893183583075 1103 36-37 0.7442400873036306 1103 38-39 0.6553735172008217 1103 40-41 0.6734288456047324 1103 42-43 0.9064562455228895 1103 44-45 0.7569858986011369 1103 46-47 0.45271923825126237 1103 48-49 0.6316012762576193 1103 50-51 0.8302531897843082 1103 52-53 0.6334901077037429 1103 54-55 0.615463832978584 1103 56-57 0.6694499841156727 1103 58-59 0.6334352298555714 1103 60-61 0.8130222441564605 1103 62-63 0.5507040124534797 1103 64-65 0.7946612095480461 1103 66-67 0.9697267668077885 1103 68-69 1.0745938430233934 1103 70-71 1.1889309589749928 1103 72-73 1.1344397444109937 1103 74-75 1.0138811405002919 1103 76-77 1.0927654794318151 1103 78-79 1.2018227620375832 1103 80-81 1.0997631617987587 1103 82-83 1.2312251823534908 1103 84-85 1.3178577398118207 1103 86-87 1.216654909876688 1103 88-89 1.2339102412532093 1103 90-91 1.3866361904373257 1103 92-93 1.3185182242327826 1103 94-95 1.4730483600829842 1103 96-97 1.6501436455404992 1103 98-99 1.813501436368508 1103 100-101 1.4872905932123306 1104 1 0.24757272939924846 1104 2 0.2348021048749267 1104 3 0.3563172858564272 1104 4 0.21282481350394278 1104 5 0.35751714533460444 1104 6 0.39016725440335875 1104 7 0.37745234208356493 1104 8 0.5351532128053194 1104 9 0.5809965850073837 1104 10-11 0.46477669301034297 1104 12-13 0.5440537787184851 1104 14-15 0.49098053276738085 1104 16-17 0.63268791572429 1104 18-19 0.5651750532003561 1104 20-21 0.6618032024339797 1104 22-23 0.6209126168045813 1104 24-25 0.6854385696603487 1104 26-27 0.7496825878596454 1104 28-29 0.813276128941105 1104 30-31 0.6990909726696302 1104 32-33 0.7149680067690696 1104 34-35 0.581117227517467 1104 36-37 0.8889590249913155 1104 38-39 1.07045656900263 1104 40-41 0.9356754672263605 1104 42-43 0.9595014782556248 1104 44-45 0.9342569196221504 1104 46-47 0.9479449326957052 1104 48-49 0.8392777026840434 1104 50-51 0.9605553669614864 1104 52-53 1.0817821497457842 1104 54-55 1.0134790356812857 1104 56-57 1.0168716245060665 1104 58-59 1.0032503574832035 1104 60-61 0.9031944476099163 1104 62-63 0.8401066963874158 1104 64-65 0.9544538146531529 1104 66-67 1.0777175701110906 1104 68-69 1.1617747739543205 1104 70-71 1.1759922390050228 1104 72-73 0.9971813923088924 1104 74-75 0.9668419137735675 1104 76-77 0.9888920747771586 1104 78-79 1.0821014782538008 1104 80-81 1.412685459096057 1104 82-83 1.4019036733444814 1104 84-85 1.3937304875595657 1104 86-87 1.441516490207018 1104 88-89 1.324551663925881 1104 90-91 1.4293351393862785 1104 92-93 1.7609098345931464 1104 94-95 1.7028367422151156 1104 96-97 1.6950948467417 1104 98-99 2.0054472884706094 1104 100-101 1.8651645609300491 1105 1 0.31700266584505243 1105 2 0.032980419479198275 1105 3 0.14291455236622497 1105 4 0.04806151990114671 1105 5 0.1052232016757273 1105 6 0.029405786059506056 1105 7 0.25690141076168516 1105 8 0.29870727235714156 1105 9 0.1905341127711111 1105 10-11 0.25771426035800715 1105 12-13 0.27113152265444995 1105 14-15 0.4505245204084005 1105 16-17 0.3775729299085242 1105 18-19 0.3827556779666921 1105 20-21 0.3142521831478078 1105 22-23 0.5760257471236727 1105 24-25 0.5684251083756351 1105 26-27 0.4917431237820651 1105 28-29 0.37238881668987744 1105 30-31 0.46768476733899433 1105 32-33 0.42710797169330306 1105 34-35 0.36987614764589694 1105 36-37 0.6046636051817629 1105 38-39 0.3333076014834404 1105 40-41 0.5373044558569688 1105 42-43 0.36385371749649664 1105 44-45 0.3872860522942858 1105 46-47 0.23579328501466534 1105 48-49 0.1382999246619221 1105 50-51 0.19588044997778553 1105 52-53 0.13208878775075306 1105 54-55 0.16231976259616232 1105 56-57 0.03891286326312127 1105 58-59 0.38541360545452363 1105 60-61 0.3169207247413972 1105 62-63 0.4409288875777939 1105 64-65 0.505108530088684 1105 66-67 0.6490724125450598 1105 68-69 0.23519471545807136 1105 70-71 0.24204585873541262 1105 72-73 0.6125070388463989 1105 74-75 0.7030346132875671 1105 76-77 0.7589211304805445 1105 78-79 0.8657835533313154 1105 80-81 1.005847885151077 1105 82-83 0.8173067696873666 1105 84-85 0.996068344639589 1105 86-87 1.0107756522372355 1105 88-89 0.9935877540233022 1105 90-91 1.0951588149739884 1105 92-93 0.7394640728224253 1105 94-95 0.7339018797692702 1105 96-97 1.5816329435244043 1105 98-99 1.4850031512903037 1105 100-101 1.648600954504257 1106 1 0.06047066998809214 1106 2 0.01566994468874583 1106 3 -0.15404975258737608 1106 4 -0.13202981468232622 1106 5 0.2012348984248149 1106 6 -0.198652398921773 1106 7 0.10090421597207921 1106 8 0.0760896356213081 1106 9 -0.2357970567253318 1106 10-11 -0.09892124538296088 1106 12-13 -0.07396785278304208 1106 14-15 0.06626060060939665 1106 16-17 -0.24311688481142824 1106 18-19 -0.21309176459225654 1106 20-21 0.06010468222408605 1106 22-23 -0.021172413493786735 1106 24-25 -0.15814024727600184 1106 26-27 -0.16088438142482886 1106 28-29 0.13620285847323288 1106 30-31 -0.31001123219567006 1106 32-33 -0.058814089197738895 1106 34-35 -0.36135805899975537 1106 36-37 -0.05817079420643978 1106 38-39 0.062352303079514115 1106 40-41 0.10213264333015815 1106 42-43 -0.051526621838782205 1106 44-45 -0.07185695725228669 1106 46-47 -0.22677328420112985 1106 48-49 -0.12166785904339861 1106 50-51 -0.42421613280797743 1106 52-53 -0.3702557177317658 1106 54-55 -0.28384947936910265 1106 56-57 -1.183567619571484 1106 58-59 -0.7346971884583482 1106 60-61 -0.29106368135576943 1106 62-63 -0.5928512920640756 1106 64-65 -0.37319365664023607 1106 66-67 -0.6899750056897318 1106 68-69 -0.8471528173250142 1106 70-71 -0.8596255020097523 1106 72-73 -0.46846432804590776 1106 74-75 -0.34165706804740736 1106 76-77 -0.15831095176684684 1106 78-79 0.0252069517207687 1106 80-81 0.08908840550306607 1106 82-83 -0.12317207258828944 1106 84-85 -0.01989273405766312 1106 86-87 -0.09524464389621556 1106 88-89 -0.03504700612922562 1106 90-91 0.1617653762844249 1106 92-93 -0.02366948731609142 1106 94-95 -0.08112542872918382 1106 96-97 0.21267044173148975 1106 98-99 0.27751309412191816 1106 100-101 -0.10565164266184723 1107 1 0.3595560362354995 1107 2 0.28696824159988665 1107 3 0.37728310461636383 1107 4 0.32108196771698516 1107 5 0.1527277971629033 1107 6 0.35037194438746155 1107 7 0.4520747586177478 1107 8 0.2266774576383881 1107 9 0.5635085086957758 1107 10-11 0.6233617486542258 1107 12-13 0.6076964655229418 1107 14-15 0.6988004850726313 1107 16-17 0.7631032575366845 1107 18-19 0.7606738608315098 1107 20-21 0.9548278447233258 1107 22-23 0.8376008123530738 1107 24-25 0.7823930609163128 1107 26-27 0.5980033350774505 1107 28-29 0.7658297855388767 1107 30-31 0.862569708758663 1107 32-33 0.9752273469916446 1107 34-35 0.9621535947670665 1107 36-37 0.8327934844507752 1107 38-39 0.9597649950757585 1107 40-41 0.9338869139672141 1107 42-43 1.0978907866817025 1107 44-45 1.0192628814663536 1107 46-47 0.8996788357163723 1107 48-49 1.0375090222388934 1107 50-51 0.7951141904908994 1107 52-53 1.1894431195391135 1107 54-55 1.0312403631852618 1107 56-57 0.9483600982739517 1107 58-59 1.0482374401541108 1107 60-61 1.115237372888128 1107 62-63 1.4457307027467152 1107 64-65 1.1700490054321655 1107 66-67 1.3032243268678485 1107 68-69 1.2390800203772159 1107 70-71 1.3990148940129714 1107 72-73 1.5047579026427549 1107 74-75 1.3821092031217397 1107 76-77 1.5654520906754108 1107 78-79 1.6224432906544344 1107 80-81 1.5742514368385052 1107 82-83 1.3090976320090313 1107 84-85 1.5833916560810266 1107 86-87 1.863788946486828 1107 88-89 1.663407983480731 1107 90-91 1.1089853778600443 1107 92-93 1.2370376167871058 1107 94-95 1.2026380140752124 1107 96-97 1.6306820883951154 1107 98-99 1.8050796414117904 1107 100-101 1.6287029154610515 1108 1 0.159069453395972 1108 2 -0.0044996969268744635 1108 3 0.11855111059025347 1108 4 0.1682660501951858 1108 5 0.24628659660405106 1108 6 0.2251129955991047 1108 7 0.23407601120723598 1108 8 0.3176829015850018 1108 9 0.2947557987665945 1108 10-11 0.3323033105369646 1108 12-13 0.3618516902663984 1108 14-15 0.5009876990245488 1108 16-17 0.527676654463022 1108 18-19 0.700489344764641 1108 20-21 0.566850294981073 1108 22-23 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-2.101996776563066 2316 66-67 -2.2303166359699915 2316 68-69 -2.1387742125321623 2316 70-71 -2.573786054913896 2316 72-73 -2.351541158366782 2316 74-75 -2.1976639983885953 2316 76-77 -2.8349254927904077 2316 78-79 -3.204817862962418 2316 80-81 -3.036174338201242 2316 82-83 -3.1021081509798414 2316 84-85 -3.129928718521512 2316 86-87 -3.541982242901092 2316 88-89 -3.5100133698579015 2316 90-91 -3.1979558234515615 2316 92-93 -3.540335942433881 2316 94-95 -4.0091565010281265 2316 96-97 -3.9284587850150583 2316 98-99 -4.087627035853714 2316 100-101 -3.5376226012321155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4383.0 3 1478.0 4 1579.0 5 1795.0 6 2071.0 7 2565.0 8 3260.0 9 4140.0 10 5340.0 11 7050.0 12 9001.0 13 10700.0 14 11643.0 15 13100.0 16 15373.0 17 17596.0 18 16805.0 19 14737.0 20 15258.0 21 16755.0 22 18568.0 23 20326.0 24 22493.0 25 25413.0 26 28389.0 27 31991.0 28 36327.0 29 41056.0 30 47430.0 31 55247.0 32 64483.0 33 77036.0 34 92819.0 35 113109.0 36 133685.0 37 138535.0 38 69313.0 39 3448.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42107563726069 19.99768885450083 14.824826056993182 22.756409451245297 2 14.204015399096606 20.584263786223122 43.98750436902847 21.2242164456518 3 18.683986399940984 26.75256677642388 30.380255101698665 24.183191721936467 4 11.813834179300285 17.719871072121652 38.45454202520716 32.011752723370904 5 11.65197220058051 37.9956709121695 34.60863351948467 15.74372336776532 6 28.257014217850596 36.57894913288209 20.558975081046107 14.605061568221208 7 26.737751586931342 33.05574423499398 22.77341744481231 17.433086733262364 8 24.613167100924546 37.713684609012965 21.026707906508506 16.64644038355398 9 26.085882315499255 18.002757105073176 22.58506238662117 33.326298192806405 10-11 23.654694761445004 26.597065001811597 30.29264114233296 19.455599094410434 12-13 25.976905954135287 24.781823010436135 29.607101297626336 19.63416973780224 14-15 20.968902356800832 29.111377454145863 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40-41 21.326828434766114 27.480683609054424 32.17763750560677 19.014850450572695 42-43 19.474769539514572 29.376403995597673 31.909165986829873 19.239660478057882 44-45 19.16084554359604 29.701041856819632 30.93553584198477 20.20257675759956 46-47 20.731305455774184 28.382386130252062 30.596827754904478 20.289480659069277 48-49 20.050619661698207 28.938745603751464 30.42576620331603 20.5848685312343 50-51 19.07323476029082 30.222051025470865 30.470165360709927 20.234548853528388 52-53 19.264575778472533 29.209462715675965 31.03150567983175 20.49445582601975 54-55 19.60780373019811 28.55630153935047 30.987524834142473 20.84836989630895 56-57 20.151499481421403 29.226352753198043 29.168570075523636 21.45357768985692 58-59 20.062868800426102 29.81352077006953 28.922328096918616 21.201282332585752 60-61 19.601581546723214 28.049087913864163 31.828879056566006 20.520451482846617 62-63 20.11294912097648 28.060880983045912 31.104595173535337 20.721574722442273 64-65 20.592645130982255 29.035282118359035 29.461178030101614 20.910894720557096 66-67 20.383425160371083 30.200844342598938 28.939354797650445 20.476375699379535 68-69 20.755890926478457 28.035986591490136 31.014883177980966 20.19323930405044 70-71 21.41073114185373 28.02901684943887 30.189031657714416 20.37122035099298 72-73 20.48023799582988 29.435442500463786 29.45043449565965 20.633885008046683 74-75 19.953256018570283 29.664001983331804 30.505284766830787 19.877457231267126 76-77 20.237727331733687 28.547296306997815 30.954678123809167 20.260298237459327 78-79 19.981377230817667 28.293085878263696 30.586265540257084 21.139271350661552 80-81 20.143898747532432 29.12067691444577 30.240968647489936 20.494455690531865 82-83 19.876355527680794 28.878888958203632 30.920035956207787 20.32471955790779 84-85 19.885684236616548 28.681349415023494 30.766113230550747 20.66685311780921 86-87 19.049894025127475 30.15150512791096 29.225031005291623 21.57356984166994 88-89 19.91163896362363 28.666953692752532 30.17304490003786 21.24836244358597 90-91 20.116369112808016 28.580959372998898 30.88618685312975 20.416484661063336 92-93 19.551988475156886 29.298479616899474 30.61089625337904 20.538635654564604 94-95 19.62498408358297 29.447298230094425 29.996624044177274 20.93109364214533 96-97 20.84884936033691 28.84027165524997 29.457338691606793 20.85354029280633 98-99 20.7796509665548 27.950523583669113 30.640595385361436 20.629230064414653 100-101 20.304800130718405 27.92606030643411 31.157737733627727 20.61140182921975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 893.0 1 613.5 2 1001.5 3 1368.0 4 786.5 5 600.5 6 912.5 7 1510.0 8 2028.0 9 2085.0 10 1727.5 11 1419.5 12 1425.0 13 1527.5 14 1670.0 15 1809.0 16 1955.5 17 2194.0 18 2428.0 19 2646.0 20 2970.5 21 3399.0 22 3941.5 23 4592.5 24 5412.5 25 6498.0 26 7948.0 27 9711.0 28 11675.5 29 14001.5 30 17188.0 31 21682.5 32 27936.0 33 36415.5 34 47106.5 35 57890.0 36 65649.0 37 67634.5 38 63082.0 39 55195.0 40 48379.0 41 44016.0 42 40726.0 43 38146.5 44 36259.5 45 34853.5 46 33760.0 47 33074.5 48 32913.0 49 31854.0 50 29908.0 51 27945.5 52 25865.0 53 23900.5 54 22563.0 55 20930.5 56 18444.0 57 16210.0 58 14243.5 59 12230.0 60 10084.0 61 8144.5 62 6588.0 63 5235.0 64 4129.0 65 3296.0 66 2514.0 67 1956.5 68 1570.0 69 1122.0 70 821.0 71 627.0 72 524.0 73 401.0 74 292.5 75 250.5 76 172.0 77 104.5 78 67.5 79 33.5 80 18.0 81 12.0 82 6.0 83 4.0 84 4.0 85 2.0 86 2.0 87 3.5 88 2.0 89 0.5 90 0.5 91 1.5 92 1.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007033426358770054 2 0.10382677005803415 3 0.11395825326531006 4 0.11571660985500257 5 0.1589219432017329 6 0.09620722483603325 7 0.027296392773321878 8 0.025621767449805204 9 0.02436579845716769 10-11 0.05019689407241248 12-13 0.05492771061134709 14-15 0.02913848062919023 16-17 0.03232026874387192 18-19 0.037846532311476964 20-21 0.059616661517193796 22-23 0.032445865643135674 24-25 0.051159803633434565 26-27 0.058193229992204616 28-29 0.053336816554006246 30-31 0.06761299743698594 32-33 0.051201669266522476 34-35 0.063049643430403 36-37 0.05291816022312708 38-39 0.03261332817548734 40-41 0.03579511629016903 42-43 0.03206907494534442 44-45 0.006949695092594221 46-47 0.013271405688869685 48-49 0.008121932819055896 50-51 0.007493948322737141 52-53 0.009670961243308825 54-55 0.01042454263889133 56-57 0.014024987084452193 58-59 0.018672072357210977 60-61 0.02310982946453018 62-63 0.010968795869034252 64-65 0.009084842380077987 66-67 0.009503498710957157 68-69 0.010885064602858418 70-71 0.030980568485058577 72-73 0.027338258406409795 74-75 0.029012883729926477 76-77 0.023612217061585183 78-79 0.0050238759705500385 80-81 0.0045633540065829524 82-83 0.00556812920069296 84-85 0.0051076072367258725 86-87 0.012224764861671763 88-89 0.03726041344824612 90-91 0.03546019122546569 92-93 0.02968273385933315 94-95 0.04697324032464287 96-97 0.04224242378570825 98-99 0.026291617579211873 100-101 0.07468828942884391 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1194297.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.07864035999606 #Duplication Level Percentage of deduplicated Percentage of total 1 88.88729781909227 65.84650167712398 2 7.298116779932018 10.812691364916729 3 1.6563297221708149 3.6809596141879197 4 0.6959049094777986 2.062067580558458 5 0.3674244901462893 1.360915333250094 6 0.2247515472140183 0.9989573421851962 7 0.1529490223610033 0.7931178934625631 8 0.11006597218882899 0.6522830055719673 9 0.08118450167218653 0.5412633751961465 >10 0.4693541227080345 6.3430871503792785 >50 0.03508890281373377 1.7908279998528354 >100 0.019378998536707253 2.76921680762443 >500 0.0010199423777306172 0.5595337451976199 >1k 0.0011332693085895746 1.7885771104929167 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4853 0.40634783475132236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4028 0.33726954015625926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2649 0.22180412409978423 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2045 0.17123043932958049 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1614 0.13514226360779605 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1465 0.12266630494759678 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1293 0.10826452716535334 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1219 0.10206841346834164 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.373126617583398E-5 0.0 22-23 0.0 0.0 0.0 5.442532301429209E-4 0.0 24-25 0.0 0.0 0.0 0.0012141033595495928 0.0 26-27 8.373126617583398E-5 0.0 0.0 0.0025956692514508533 0.0 28-29 1.2559689926375098E-4 0.0 0.0 0.005233204135989624 0.0 30-31 1.6746253235166796E-4 0.0 0.0 0.01226663049475968 0.0 32-33 1.6746253235166796E-4 0.0 0.0 0.030938702851970656 0.0 34-35 1.6746253235166796E-4 8.373126617583398E-5 0.0 0.05861188632308379 0.0 36-37 1.6746253235166796E-4 8.373126617583398E-5 0.0 0.10458035145361665 0.0 38-39 2.5119379852750197E-4 8.373126617583398E-5 0.0 0.169974470336943 0.0 40-41 2.5119379852750197E-4 8.373126617583398E-5 0.0 0.2451651473628419 0.0 42-43 2.5119379852750197E-4 8.373126617583398E-5 0.0 0.32458425333062046 0.0 44-45 2.5119379852750197E-4 8.373126617583398E-5 0.0 0.4225079691232583 0.0 46-47 2.5119379852750197E-4 8.373126617583398E-5 0.0 0.5379733851797334 0.0 48-49 2.5119379852750197E-4 8.373126617583398E-5 0.0 0.6677149821191881 0.0 50-51 2.5119379852750197E-4 8.373126617583398E-5 0.0 0.7994661294468629 0.0 52-53 2.5119379852750197E-4 8.373126617583398E-5 0.0 0.9395485377590331 0.0 54-55 2.5119379852750197E-4 8.373126617583398E-5 0.0 1.1028663724349972 0.0 56-57 2.5119379852750197E-4 8.373126617583398E-5 0.0 1.2903825430357774 0.0 58-59 2.5119379852750197E-4 8.373126617583398E-5 0.0 1.4882395250092733 0.0 60-61 2.5119379852750197E-4 8.373126617583398E-5 0.0 1.7106716336053762 0.0 62-63 2.5119379852750197E-4 8.373126617583398E-5 0.0 1.9370391117117434 0.0 64-65 2.5119379852750197E-4 8.373126617583398E-5 0.0 2.167048899896759 0.0 66-67 2.5119379852750197E-4 8.373126617583398E-5 0.0 2.4323095511418016 0.0 68-69 2.5119379852750197E-4 8.373126617583398E-5 0.0 2.7544655977533226 0.0 70-71 2.5119379852750197E-4 8.373126617583398E-5 0.0 3.07951037304791 0.0 72-73 2.5119379852750197E-4 8.373126617583398E-5 0.0 3.3885206108698256 0.0 74-75 2.5119379852750197E-4 8.373126617583398E-5 0.0 3.7134397892651494 0.0 76-77 2.5119379852750197E-4 8.373126617583398E-5 0.0 4.080726988345445 0.0 78-79 2.5119379852750197E-4 8.373126617583398E-5 0.0 4.472631179681436 0.0 80-81 2.5119379852750197E-4 8.373126617583398E-5 0.0 4.877764911073209 0.0 82-83 2.5119379852750197E-4 8.373126617583398E-5 0.0 5.290518187686983 0.0 84-85 2.5119379852750197E-4 8.373126617583398E-5 0.0 5.7026853454375255 0.0 86-87 2.5119379852750197E-4 8.373126617583398E-5 0.0 6.120755557453464 0.0 88-89 2.5119379852750197E-4 8.373126617583398E-5 0.0 6.5770490924786715 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3200 0.0 44.12606 1 TACCTGG 1280 0.0 42.71462 2 GTACCTG 1380 0.0 39.619354 1 GTACGAC 85 3.6149868E-7 39.153244 3 GTATCAA 5055 0.0 35.175404 1 ACCTGGG 1520 0.0 32.529575 3 TACGACG 110 2.7142505E-6 30.254782 4 GAACCGA 80 4.4896608E-4 29.69211 6 GTACATG 5405 0.0 29.379162 1 TAGGACC 1500 0.0 29.159845 4 TACATGG 5445 0.0 28.377499 2 TATCAAC 6460 0.0 27.525026 2 ATCAACG 6410 0.0 27.443047 3 TAACGCA 105 6.863421E-5 27.167557 4 TCAACGC 6495 0.0 27.010702 4 TGTACGA 125 7.3391693E-6 26.624208 2 ACATGGG 5340 0.0 26.620646 3 CAACGCA 6630 0.0 26.53242 5 AACGCAG 6755 0.0 26.232792 6 CGACGTG 150 1.0587355E-6 25.337265 6 >>END_MODULE