FastQCFastQC Report
Thu 26 May 2016
SRR938543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194297
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49260.4124602171821582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.36381235153399866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28080.23511739542174184No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.15850328687085372No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16740.1401661395783461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14070.11780989150939841No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC13440.11253482174032087No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA12990.10876691476240834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12700.040.0388342
GGTATCA38350.038.4147261
TACGACC550.002576794734.55334
GTACCTG15300.034.1666681
ACCTGGG14700.033.6130753
TATAACG1308.249117E-932.9003522
GTATCAA58300.032.850191
TACGACG902.4092995E-531.6738574
ATAACGC1303.0310002E-729.2374083
TAGGACC15150.028.8514354
CGACGCG350.00836865327.15925872-73
CGGTTCG350.00839948827.13878842-43
ATCAACG70700.026.745823
CGACGTG1257.4070485E-626.5926726
TCAACGC71500.026.5130124
TATCAAC72200.026.2625622
TAACGCA1457.872386E-726.2128494
CAACGCA73300.025.926765
CTGTACG1109.4461546E-525.921491
TACAACG1109.4461546E-525.921492
Produced by FastQC (version 0.11.5)