FastQCFastQC Report
Thu 26 May 2016
SRR938542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180572
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49330.41784829726607103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.3314495007504837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26210.222011025164073No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19660.16652944504867132No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15390.13036053709557738No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC13870.11748542232070557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13040.1104549320160058No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA12070.10223857587677838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13150.046.637072
GTACCTG15600.039.3577271
ACCTGGG14750.039.3219533
CGACGTC500.001609017438.032714
GGTATCA33800.037.7383581
GTATCAA51650.035.6619871
TACGACG902.3988674E-531.6939244
GTACGAC1252.1378219E-730.4261683
TAACGCA650.00581961429.2559324
TATCAAC64050.028.8733862
GTACATG52900.028.5212481
TACATGG53250.028.3906142
ATCAACG64900.028.3487283
TGTACGA1354.193589E-728.1723772
TCAACGC65400.028.1319924
CAACGCA66150.027.7411675
AACGCAG67700.027.1693386
TAGGACC13850.027.1171864
ACATGGG53350.026.8225063
TAACCGT908.902852E-426.4116045
Produced by FastQC (version 0.11.5)