##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938542_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1180572 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.01409740363146 31.0 31.0 34.0 28.0 34.0 2 31.121757927513105 31.0 31.0 34.0 27.0 34.0 3 31.170132783091585 31.0 31.0 34.0 27.0 34.0 4 34.79672480797444 37.0 35.0 37.0 32.0 37.0 5 34.83616416448976 37.0 35.0 37.0 32.0 37.0 6 34.87327075349915 37.0 35.0 37.0 32.0 37.0 7 34.81207668824943 37.0 35.0 37.0 32.0 37.0 8 34.77850990875609 37.0 35.0 37.0 32.0 37.0 9 36.27366225863395 39.0 35.0 39.0 32.0 39.0 10-11 36.09508356965945 39.0 35.0 39.0 30.5 39.0 12-13 35.95822957007281 38.0 35.0 39.0 30.0 39.0 14-15 36.912854531532176 39.0 36.0 41.0 31.0 41.0 16-17 36.7909602294481 39.0 36.0 41.0 30.0 41.0 18-19 36.82752132017362 39.0 36.0 41.0 30.5 41.0 20-21 36.949444421856526 39.0 36.0 41.0 30.5 41.0 22-23 36.84906723181644 39.0 36.0 41.0 30.0 41.0 24-25 36.749379961577944 39.0 36.0 41.0 30.0 41.0 26-27 36.57707746753269 39.0 36.0 41.0 30.0 41.0 28-29 36.388951711543214 39.0 35.0 41.0 29.5 41.0 30-31 36.13918634356905 39.0 35.0 40.0 29.0 41.0 32-33 35.840983015013066 38.0 35.0 40.0 27.0 41.0 34-35 35.61854634871909 38.0 35.0 40.0 27.0 41.0 36-37 35.30023539436816 38.0 34.0 40.0 25.5 41.0 38-39 35.03098413311513 38.0 34.0 40.0 25.0 41.0 40-41 34.89862456504135 38.0 34.0 40.0 25.0 41.0 42-43 34.76664743869921 38.0 34.0 40.0 24.0 41.0 44-45 34.4796950122483 38.0 33.0 40.0 24.0 41.0 46-47 34.05065341207482 38.0 33.0 40.0 22.5 41.0 48-49 33.691357240388555 38.0 33.0 40.0 19.5 41.0 50-51 33.00086187034759 37.5 32.0 39.5 18.0 40.5 52-53 33.10202512002657 37.5 32.5 39.5 18.0 40.5 54-55 33.28275700253775 38.0 32.5 40.0 16.5 41.0 56-57 32.986741172922954 38.0 32.0 40.0 13.5 41.0 58-59 32.67828222251586 37.0 31.5 40.0 10.5 41.0 60-61 32.29587818447329 37.0 31.0 40.0 8.0 41.0 62-63 31.819931778832633 36.0 30.5 39.0 7.5 41.0 64-65 31.34077887668012 36.0 30.0 39.0 2.0 40.0 66-67 30.78873884862592 35.0 29.0 39.0 2.0 40.0 68-69 30.233990811233877 35.0 28.0 38.0 2.0 40.0 70-71 29.645046638409177 34.5 28.0 38.0 2.0 40.0 72-73 29.100807066405096 34.0 27.0 37.0 2.0 39.0 74-75 28.510740556272722 34.0 26.0 36.5 2.0 39.0 76-77 27.762219500377782 33.5 25.5 36.0 2.0 39.0 78-79 26.996907431312955 32.5 24.0 35.5 2.0 38.0 80-81 26.31768075136459 32.0 22.0 35.0 2.0 37.0 82-83 25.680540873407125 32.0 20.5 35.0 2.0 36.5 84-85 25.004769721795874 31.0 18.0 35.0 2.0 36.0 86-87 24.288107375069035 31.0 10.5 34.0 2.0 36.0 88-89 23.44973411193896 30.5 4.5 34.0 2.0 35.0 90-91 22.613923589582 29.5 2.0 34.0 2.0 35.0 92-93 21.808260741403316 29.0 2.0 34.0 2.0 35.0 94-95 20.817935288995503 29.0 2.0 34.0 2.0 35.0 96-97 19.4248144119969 26.5 2.0 33.5 2.0 35.0 98-99 17.922045415273274 24.5 2.0 33.0 2.0 34.5 100-101 14.608939564888885 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6557383411864066 1101 2 0.7367477609536408 1101 3 0.6736967902147875 1101 4 0.6226027862240002 1101 5 0.47855882656170223 1101 6 0.4559477144876709 1101 7 0.4366512497856547 1101 8 0.5344644937314271 1101 9 0.7003777598878003 1101 10-11 0.7575293742122184 1101 12-13 0.6664852377621813 1101 14-15 0.9389035126724323 1101 16-17 0.9015535016469798 1101 18-19 0.7028400906372099 1101 20-21 0.8186652275880206 1101 22-23 0.8145323704522127 1101 24-25 1.0978181904739088 1101 26-27 0.987039830652968 1101 28-29 1.0489640514616454 1101 30-31 1.0431130717283281 1101 32-33 0.9181712977856265 1101 34-35 0.9125010718198396 1101 36-37 0.956686629994195 1101 38-39 0.9984056890840236 1101 40-41 0.9587444984992928 1101 42-43 0.9463108552075354 1101 44-45 0.9194430154788336 1101 46-47 1.0707770242633003 1101 48-49 1.0887176412046742 1101 50-51 1.0644785006981365 1101 52-53 1.2885696033216405 1101 54-55 1.363340760024066 1101 56-57 1.3556607805509913 1101 58-59 1.327619156836839 1101 60-61 1.3857305822175192 1101 62-63 1.136950177555331 1101 64-65 1.3013364682650455 1101 66-67 1.212221270691753 1101 68-69 1.4117817894112115 1101 70-71 1.5944342387683221 1101 72-73 1.5869862976452822 1101 74-75 1.3767229388299 1101 76-77 1.2015614347224925 1101 78-79 1.2017794994277224 1101 80-81 1.3283453950759636 1101 82-83 1.265142500106613 1101 84-85 1.3509959774256473 1101 86-87 1.2628802446692902 1101 88-89 1.4977816084565454 1101 90-91 1.977353223543286 1101 92-93 1.9663337417717592 1101 94-95 1.891623955742105 1101 96-97 1.8815750523738082 1101 98-99 2.131913298829261 1101 100-101 2.0552409838743095 1102 1 0.6257560159719517 1102 2 0.719337017020397 1102 3 0.5551997150757444 1102 4 0.590524930753979 1102 5 0.6436904513057584 1102 6 0.5426469782916428 1102 7 0.4924136379380286 1102 8 0.6066398685704968 1102 9 0.6294593831516266 1102 10-11 0.6684261315411746 1102 12-13 0.7979135508643296 1102 14-15 0.943108575390319 1102 16-17 0.8266212923307847 1102 18-19 0.7089350512029853 1102 20-21 0.7890617620818645 1102 22-23 0.8399119702771429 1102 24-25 0.7588587704693168 1102 26-27 0.5494120605163957 1102 28-29 0.7697664499196435 1102 30-31 0.8199358990059054 1102 32-33 0.7312074322804207 1102 34-35 0.7791528561410459 1102 36-37 0.8989183257094382 1102 38-39 0.793740233808272 1102 40-41 0.671328401722441 1102 42-43 0.8768311051989244 1102 44-45 1.057454366067688 1102 46-47 0.8877893854114163 1102 48-49 1.03966311021059 1102 50-51 0.8207876950271711 1102 52-53 0.7266490446415901 1102 54-55 0.6930559231337696 1102 56-57 0.7574977100510836 1102 58-59 0.9379209403979232 1102 60-61 0.7890125394539851 1102 62-63 0.7244511677113756 1102 64-65 0.553381790607844 1102 66-67 0.7536345974320433 1102 68-69 0.8806996908724791 1102 70-71 0.8975836151100332 1102 72-73 1.0103210151967659 1102 74-75 0.7090413122055459 1102 76-77 0.6603861454969113 1102 78-79 0.7661968516024231 1102 80-81 0.8059066587311108 1102 82-83 1.0659995151721873 1102 84-85 1.086125433827977 1102 86-87 1.0641573259372983 1102 88-89 1.1119664039803219 1102 90-91 1.4882997487156508 1102 92-93 1.3634427261476993 1102 94-95 1.3416544805528154 1102 96-97 1.5832632384319325 1102 98-99 1.489699285900823 1102 100-101 1.3408159904953543 1103 1 0.6673398235204786 1103 2 0.5633103312427394 1103 3 0.6508219059736611 1103 4 0.40665916331639096 1103 5 0.6203989311751457 1103 6 0.6659982993705285 1103 7 0.44244345931038254 1103 8 0.5869371963540502 1103 9 0.46333693675494914 1103 10-11 0.643128194673892 1103 12-13 0.5655504800065287 1103 14-15 0.7928274476520158 1103 16-17 0.8856264766616775 1103 18-19 0.7985170299182798 1103 20-21 0.7325134791442665 1103 22-23 0.8056668306862349 1103 24-25 0.8107008928018047 1103 26-27 0.6983278914727222 1103 28-29 0.7228986432380751 1103 30-31 0.7873115549574408 1103 32-33 0.8918457520319762 1103 34-35 0.895147711361048 1103 36-37 0.8134508262591993 1103 38-39 0.7317220625465879 1103 40-41 0.7990050083192131 1103 42-43 0.968635448519656 1103 44-45 0.9954611118677832 1103 46-47 1.0727621823080895 1103 48-49 1.0229018378109416 1103 50-51 0.8779418976258171 1103 52-53 0.9888402115036357 1103 54-55 0.8888569631640237 1103 56-57 0.937859663991695 1103 58-59 0.8833486200103877 1103 60-61 0.9679376094986196 1103 62-63 1.166793086752289 1103 64-65 0.9647585255077153 1103 66-67 1.0320159218173863 1103 68-69 1.1123789268125321 1103 70-71 1.385684729337754 1103 72-73 1.2749655238718596 1103 74-75 1.1638231732173843 1103 76-77 1.171134495829218 1103 78-79 0.9604154604551809 1103 80-81 1.2158599570381767 1103 82-83 1.37763918440017 1103 84-85 1.4764836383440567 1103 86-87 1.512455213761477 1103 88-89 1.3524730034421104 1103 90-91 1.4998205279631414 1103 92-93 1.5123138645963827 1103 94-95 1.2852758709406835 1103 96-97 1.4160044949163009 1103 98-99 1.8566825179047726 1103 100-101 1.398649281904742 1104 1 0.34575192651664466 1104 2 0.5021060665577366 1104 3 0.4937480194777102 1104 4 0.6338151722149519 1104 5 0.5089627357853601 1104 6 0.6236502376485618 1104 7 0.5225275933568625 1104 8 0.4987842995868803 1104 9 0.5029678001051678 1104 10-11 0.5479375026683968 1104 12-13 0.6673485346285801 1104 14-15 0.6712620337267055 1104 16-17 0.7416083067842649 1104 18-19 0.7297676215782474 1104 20-21 0.8221935981862529 1104 22-23 0.8301674696789902 1104 24-25 0.7450480847836332 1104 26-27 0.789307443704466 1104 28-29 0.7247912155389216 1104 30-31 0.6853367419219865 1104 32-33 0.7107578668613428 1104 34-35 0.8094953175048474 1104 36-37 1.0634212432611392 1104 38-39 1.0133226210735913 1104 40-41 0.9479117686259357 1104 42-43 0.9744233620899649 1104 44-45 0.9997247614630851 1104 46-47 1.0865441615289981 1104 48-49 0.9649916399598482 1104 50-51 0.6723030823657226 1104 52-53 0.8613019902701424 1104 54-55 0.9385209003060666 1104 56-57 1.1655563021797946 1104 58-59 0.9703470402782912 1104 60-61 1.0385322573426095 1104 62-63 1.074738192541087 1104 64-65 1.0791781662517828 1104 66-67 1.194179563129449 1104 68-69 0.9766210695682496 1104 70-71 0.9589056078344669 1104 72-73 0.8618352580982034 1104 74-75 0.5465362003864094 1104 76-77 0.9815045199689436 1104 78-79 1.229841136130858 1104 80-81 1.078429181253636 1104 82-83 1.26039436734143 1104 84-85 1.141138907667667 1104 86-87 1.2208326750669158 1104 88-89 1.378899495608536 1104 90-91 1.3504152941391219 1104 92-93 1.4355067871599267 1104 94-95 1.1397578430616626 1104 96-97 1.0611172357219054 1104 98-99 0.6499262048929886 1104 100-101 0.7224041335164237 1105 1 0.49464952311407373 1105 2 0.5275170416460639 1105 3 0.4966735094972883 1105 4 0.4526963973201603 1105 5 0.3859219702071286 1105 6 0.5493441976368558 1105 7 0.4943120860093799 1105 8 0.5198302466580174 1105 9 0.53689574866155 1105 10-11 0.6153707508624748 1105 12-13 0.6616649263741934 1105 14-15 0.8175970135858321 1105 16-17 0.7209614499982493 1105 18-19 0.614928776696722 1105 20-21 0.6502644484911855 1105 22-23 0.768515580487545 1105 24-25 0.8925128241899785 1105 26-27 0.9186000377139578 1105 28-29 0.8125007115670044 1105 30-31 0.9760520382966291 1105 32-33 0.9221364759033506 1105 34-35 0.8529441186165272 1105 36-37 0.9472764765127053 1105 38-39 0.7606362336036838 1105 40-41 0.8390416969514263 1105 42-43 1.106889690825028 1105 44-45 1.0787020917284025 1105 46-47 0.9199877867737243 1105 48-49 0.6754954149671661 1105 50-51 0.3998858118479163 1105 52-53 0.5164598542123571 1105 54-55 0.8129048596192519 1105 56-57 0.7839398423602688 1105 58-59 0.7522844601086192 1105 60-61 1.0439250747020168 1105 62-63 1.0798697552028322 1105 64-65 1.0064677841377794 1105 66-67 1.1760844228184517 1105 68-69 1.1903892976214472 1105 70-71 1.218451425508924 1105 72-73 1.0158535170940866 1105 74-75 1.0133097365162094 1105 76-77 1.1054988827747145 1105 78-79 1.226443770197065 1105 80-81 1.2204223401077456 1105 82-83 1.4760838547055606 1105 84-85 1.408230168891425 1105 86-87 1.4658554741982712 1105 88-89 1.4156158018398308 1105 90-91 1.476350958675436 1105 92-93 1.456032686185516 1105 94-95 1.3467661497655392 1105 96-97 1.364341111658625 1105 98-99 1.305065432901177 1105 100-101 1.0224096188102116 1106 1 0.4340285329986422 1106 2 0.47559350627961905 1106 3 0.19898520687257104 1106 4 0.37713972082995895 1106 5 0.5013859162272141 1106 6 0.013541737031758316 1106 7 0.2683599921456974 1106 8 0.3188379891685784 1106 9 0.4993405992528608 1106 10-11 0.3146562701911151 1106 12-13 0.31766113976483723 1106 14-15 0.6047360160077417 1106 16-17 0.4345673316805332 1106 18-19 0.08100752849760795 1106 20-21 0.3541084097820857 1106 22-23 0.4316113409361435 1106 24-25 0.4633155093658843 1106 26-27 0.16208696596530814 1106 28-29 0.18813616058771032 1106 30-31 0.41460842802717934 1106 32-33 0.6303419478302743 1106 34-35 0.5458791209140657 1106 36-37 0.253448718869528 1106 38-39 0.3455167446577505 1106 40-41 0.5244891629289512 1106 42-43 0.17986311200585448 1106 44-45 0.5104588228065836 1106 46-47 0.30095869276146914 1106 48-49 0.6298740275341501 1106 50-51 0.2719547309022303 1106 52-53 0.7965100198765143 1106 54-55 0.4626770066198489 1106 56-57 -0.09044802943397201 1106 58-59 -0.4106271522752536 1106 60-61 0.48463976266950937 1106 62-63 0.7107852094750307 1106 64-65 0.8877537802475182 1106 66-67 0.2772463176433 1106 68-69 -7.795416238565167E-4 1106 70-71 0.3145563311719073 1106 72-73 0.7982274724079517 1106 74-75 0.8121205477005269 1106 76-77 0.9202773695820419 1106 78-79 1.0579258338991622 1106 80-81 1.2002358426868511 1106 82-83 1.2811854125360007 1106 84-85 1.0897016950401053 1106 86-87 1.0116903766481649 1106 88-89 0.9851978582865648 1106 90-91 1.0733942248333044 1106 92-93 1.0578247612543556 1106 94-95 1.0894750265314421 1106 96-97 1.1784279203692059 1106 98-99 0.9139860764865695 1106 100-101 0.18722904660378603 1107 1 0.3640278637441945 1107 2 0.508132657664973 1107 3 0.3269433115439675 1107 4 0.5606024433570624 1107 5 0.40008087004866155 1107 6 0.3767561661577119 1107 7 0.3520502611859868 1107 8 0.2281707424813888 1107 9 0.3411489247782242 1107 10-11 0.3327947389369328 1107 12-13 0.23737904901393136 1107 14-15 0.37830213834745763 1107 16-17 0.356830080977808 1107 18-19 0.46752372445531165 1107 20-21 0.5356771422141549 1107 22-23 0.5753069640120287 1107 24-25 0.5472249592387328 1107 26-27 0.6000855605309638 1107 28-29 0.5375990838911449 1107 30-31 0.6500354123666341 1107 32-33 0.6557284422754677 1107 34-35 0.8709845284900197 1107 36-37 0.6441644865043799 1107 38-39 0.6970192208992216 1107 40-41 0.6708166291316005 1107 42-43 0.7494070150978089 1107 44-45 0.89219152257904 1107 46-47 0.7471506349378885 1107 48-49 0.7796330959319491 1107 50-51 0.7646799350248372 1107 52-53 0.6678625831210567 1107 54-55 0.6781351112912475 1107 56-57 0.7681418514387275 1107 58-59 0.6509676810803882 1107 60-61 0.6398699862004946 1107 62-63 0.6725858385742178 1107 64-65 0.9579870154235373 1107 66-67 0.6446536259018991 1107 68-69 0.6697280878768801 1107 70-71 0.852715546805694 1107 72-73 0.44915271668582335 1107 74-75 0.7695827348240734 1107 76-77 0.7966309367082616 1107 78-79 0.49025417013260153 1107 80-81 0.4884335404515454 1107 82-83 0.41494428015747076 1107 84-85 0.43217258247150525 1107 86-87 0.18249080497101033 1107 88-89 0.3155167580322491 1107 90-91 0.4130542435724287 1107 92-93 0.4358146555121536 1107 94-95 0.2704765658166792 1107 96-97 0.32331481636706627 1107 98-99 0.5891835065494817 1107 100-101 0.5472461125922727 1108 1 0.20624972022872257 1108 2 0.18432752270776476 1108 3 0.32664377237346187 1108 4 0.3765537270963648 1108 5 0.3539255046767025 1108 6 0.3021182464737109 1108 7 0.22848870753226436 1108 8 0.2543820657203639 1108 9 0.32585596888618795 1108 10-11 0.3454395710633307 1108 12-13 0.3113405368348694 1108 14-15 0.42373169726779736 1108 16-17 0.30003942923324445 1108 18-19 0.3192139812031769 1108 20-21 0.43376799145708134 1108 22-23 0.38825627276779073 1108 24-25 0.44753601914656826 1108 26-27 0.5360779897607202 1108 28-29 0.18625115762386457 1108 30-31 0.40442975074714127 1108 32-33 0.2570961183781648 1108 34-35 0.1136951934011492 1108 36-37 0.3106440783411202 1108 38-39 0.32895141313095166 1108 40-41 0.31600919002692507 1108 42-43 0.23390833175350423 1108 44-45 0.5530703902551437 1108 46-47 0.49216709313406426 1108 48-49 0.44011367526045575 1108 50-51 0.36319869736848176 1108 52-53 0.43055185237714966 1108 54-55 0.39237638844726774 1108 56-57 0.5012261174031778 1108 58-59 0.46016122946748084 1108 60-61 0.5295309473591558 1108 62-63 0.6092728036828383 1108 64-65 0.49805778566711467 1108 66-67 0.45722768778471945 1108 68-69 0.45271525048385897 1108 70-71 0.43668690954433487 1108 72-73 0.318354494171011 1108 74-75 0.37527068742446446 1108 76-77 0.3204772171559256 1108 78-79 0.3216399502510967 1108 80-81 0.3800563185950594 1108 82-83 0.3372138654109271 1108 84-85 0.4319355844464887 1108 86-87 0.5551504494739774 1108 88-89 0.4630266329499584 1108 90-91 0.3637981540398414 1108 92-93 0.47518101495915843 1108 94-95 0.41393772447368704 1108 96-97 0.5597269295994565 1108 98-99 0.25853176198068795 1108 100-101 0.2608948946857552 1109 1 0.5039736307536309 1109 2 0.6768183880495364 1109 3 0.5368496363784523 1109 4 0.5283655085775152 1109 5 0.7388680231688127 1109 6 0.6689839447180788 1109 7 0.4915276523678429 1109 8 0.5798646430307599 1109 9 0.6072497840541757 1109 10-11 0.5167273350772277 1109 12-13 0.47506569502041174 1109 14-15 0.5629813185136712 1109 16-17 0.5851263198847008 1109 18-19 0.7313055951413219 1109 20-21 0.5298735078994596 1109 22-23 0.5917247693367216 1109 24-25 0.6078479343056387 1109 26-27 0.39185376732066146 1109 28-29 0.4789024364760195 1109 30-31 0.2586316935329904 1109 32-33 0.15800869301500597 1109 34-35 0.1321463118397901 1109 36-37 0.14105313279259235 1109 38-39 0.23623953361931882 1109 40-41 0.25345326809483026 1109 42-43 0.20465247220445093 1109 44-45 0.35666187051536724 1109 46-47 0.5745911195131796 1109 48-49 0.5232096560108417 1109 50-51 0.4991847948642487 1109 52-53 0.2911792474399775 1109 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-1.682981202973128 2316 76-77 -1.6254500940075864 2316 78-79 -1.3846044598635459 2316 80-81 -1.5021942708837592 2316 82-83 -1.4633666695919167 2316 84-85 -1.524169789974195 2316 86-87 -1.8107427125717201 2316 88-89 -1.867909215493004 2316 90-91 -2.094245589353932 2316 92-93 -2.0504194403624147 2316 94-95 -2.1058102225138704 2316 96-97 -2.1098364495370596 2316 98-99 -2.0677500495487067 2316 100-101 -2.00512022849499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3936.0 3 1319.0 4 1405.0 5 1595.0 6 1874.0 7 2277.0 8 2929.0 9 3821.0 10 4933.0 11 6288.0 12 8400.0 13 10136.0 14 10617.0 15 12448.0 16 13968.0 17 16370.0 18 15322.0 19 13279.0 20 13711.0 21 15028.0 22 16242.0 23 18412.0 24 20542.0 25 22604.0 26 25791.0 27 29327.0 28 33213.0 29 38372.0 30 44571.0 31 52097.0 32 61615.0 33 73832.0 34 91396.0 35 113498.0 36 137878.0 37 151488.0 38 84884.0 39 5154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44651447764474 20.00054310791809 14.837708322195379 22.715234092241786 2 14.191792802282688 20.642908171350655 44.01409137017982 21.151207656186838 3 18.643455917995166 26.849362896473643 30.368940398024453 24.13824078750674 4 11.838572547264794 17.73552732832294 38.47449209626187 31.95140802815039 5 11.640344379257913 38.06148515673867 34.651933870232284 15.646236593771137 6 28.257042477358546 36.67801034916787 20.52135734295074 14.54358983052284 7 26.75833515517887 33.03844856718204 22.837893031398448 17.365323246240642 8 24.594907652586972 37.62565001614184 21.092849038649746 16.686593292621442 9 26.09879074970129 18.074859965934223 22.607557178811426 33.21879210555306 10-11 23.590933434570093 26.643882746137525 30.338445450786054 19.426738368506328 12-13 26.004047926020483 24.76700275101357 29.60019421910067 19.628755103865274 14-15 20.9737799153338 29.075577631583403 27.030480509488513 22.92016194359428 16-17 19.616086264295404 30.30591762152947 30.401453329006333 19.67654278516879 18-19 20.43456228539663 27.53227736330634 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19.177373776390823 29.693720754512405 30.957778071432685 20.171127397664087 46-47 20.733848426151557 28.37946535788305 30.62825595151616 20.258430264449235 48-49 20.006785636983672 28.972170840482892 30.390716299803167 20.630327222730273 50-51 19.04717934946739 30.137060569852437 30.576845450960576 20.2389146297196 52-53 19.20208426693594 29.263898995563604 31.07107126610491 20.462945471395546 54-55 19.625555829464023 28.611334897153018 30.914072089884897 20.84903718349806 56-57 20.101137211233947 29.28921438860866 29.176124366833534 21.43352403332386 58-59 20.074251659331864 29.820449067936245 28.97356505385681 21.131734218875085 60-61 19.569058994951035 28.064704686388126 31.86858290129104 20.497653417369794 62-63 20.06486925135649 28.053409976972176 31.20767520648591 20.674045565185423 64-65 20.587348325952227 29.002356644517707 29.525401273367375 20.884893756162683 66-67 20.305532778553324 30.198478985674203 28.993763807381107 20.502224428391365 68-69 20.743283996801257 28.062693991515204 31.090867658818905 20.103154352864635 70-71 21.3545523403491 28.08211343953286 30.142278110369112 20.42105610974893 72-73 20.436724027573565 29.557239542891907 29.390379922284676 20.61565650724986 74-75 19.955419096080234 29.69226298497195 30.574519242988803 19.777798675959016 76-77 20.24061652318739 28.519492115558283 31.03942983295861 20.200461528295715 78-79 19.942525206106644 28.34240068444559 30.6334298305616 21.081644278886166 80-81 20.09778134691412 29.132708339157183 30.31022684628684 20.459283467641853 82-83 19.86117764356928 28.794923174599944 31.076326459559773 20.267572722271 84-85 19.81189533702721 28.723995415552388 30.837356301878927 20.626752945541472 86-87 18.970862115922127 30.151163918546242 29.31908255458509 21.55889141094654 88-89 19.857482990315283 28.635793629947216 30.303971327136697 21.20275205260081 90-91 20.111369228012034 28.565745170970793 30.988066130086683 20.334819470930494 92-93 19.559163298175637 29.35200427687514 30.654553372402464 20.434279052546767 94-95 19.60390141892888 29.434301033904823 30.09394281918274 20.867854727983556 96-97 20.871699077273227 28.84547514772568 29.55013521660713 20.73269055839396 98-99 20.76289513136062 28.002460350777902 30.691986364708878 20.542658153152598 100-101 20.28040644687191 27.990650856912907 31.127074648548465 20.60186804766672 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 970.0 1 642.5 2 978.5 3 1340.0 4 746.0 5 542.5 6 889.0 7 1526.0 8 2070.5 9 2168.0 10 1814.5 11 1443.0 12 1430.5 13 1537.5 14 1683.0 15 1867.5 16 1986.0 17 2183.0 18 2413.5 19 2676.0 20 3048.5 21 3378.5 22 3896.5 23 4660.5 24 5471.5 25 6532.5 26 7910.0 27 9615.0 28 11560.0 29 13730.0 30 16920.0 31 21366.0 32 27482.0 33 36018.5 34 46736.5 35 57275.5 36 65133.0 37 67262.0 38 62574.5 39 54685.5 40 47825.5 41 43550.0 42 40133.5 43 37425.0 44 35592.5 45 34394.0 46 33245.5 47 32569.5 48 32760.5 49 31648.0 50 29574.0 51 27470.0 52 25298.5 53 23558.5 54 22260.0 55 20527.0 56 18157.5 57 16093.0 58 14140.0 59 11905.0 60 9733.0 61 7951.0 62 6448.0 63 5169.5 64 4103.0 65 3201.0 66 2485.5 67 1944.0 68 1507.5 69 1081.0 70 779.5 71 604.0 72 491.5 73 389.0 74 277.0 75 205.5 76 143.5 77 81.5 78 54.5 79 42.5 80 30.0 81 15.5 82 8.0 83 5.0 84 3.0 85 2.5 86 1.5 87 0.0 88 2.0 89 2.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18372449964932255 2 0.047349928678640525 3 0.04607935814164659 4 0.052855734338947566 5 0.0601403387510461 6 0.06496850679162305 7 0.03811711610981795 8 0.034644223308701205 9 0.04252176063806359 10-11 0.04235235123313106 12-13 0.03909122018817997 14-15 0.03455951860623494 16-17 0.03261131044951091 18-19 0.041335894803535916 20-21 0.05128869734332171 22-23 0.047053462220008606 24-25 0.06861080899767232 26-27 0.05323690550004574 28-29 0.045571129926849016 30-31 0.05387219076854271 32-33 0.050102831508794035 34-35 0.04925578448413142 36-37 0.04760404278603931 38-39 0.026427867169473783 40-41 0.02473377312014854 42-43 0.022150279694927544 44-45 0.004065825718380582 46-47 0.011646896589111042 48-49 0.011646896589111042 50-51 0.00919046021758944 52-53 0.00923281256882257 54-55 0.010926906618147813 56-57 0.008174003787994294 58-59 0.010757497213215289 60-61 0.011181020725546598 62-63 0.003684654557282402 64-65 0.00677637619730097 66-67 0.003727006908515533 68-69 0.009148107866356309 70-71 0.02354790728562087 72-73 0.020202071538203515 74-75 0.020837356806700482 76-77 0.01236688656007427 78-79 0.004616406284411285 80-81 0.0068610808997672315 82-83 0.00597168152387148 84-85 0.004997577445509465 86-87 0.008343413192926818 88-89 0.03066310229278689 90-91 0.03756653554378725 92-93 0.022954974368357032 94-95 0.032696015151977176 96-97 0.036295965006793315 98-99 0.021387937372731182 100-101 0.12083125806812291 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1180572.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.8544563658232 #Duplication Level Percentage of deduplicated Percentage of total 1 88.65056304971884 65.47239140561119 2 7.5109054739907 11.094276811933375 3 1.655333743276408 3.6676132114104676 4 0.6935267371863207 2.0488016060023546 5 0.36306086434329554 1.3406831381889988 6 0.2367532599432486 1.0491169983567032 7 0.15789961072199854 0.8163112937173816 8 0.10993615914014045 0.6495420214593358 9 0.08980002571264642 0.5968918872580001 >10 0.47506270531683753 6.350356033522376 >50 0.03540244726543407 1.7989675444321063 >100 0.01956882808047763 2.7718052123014836 >500 0.0011511027914687627 0.6438965728838006 >1k 0.0010359925123218866 1.699346262922404 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4933 0.41784829726607103 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3913 0.3314495007504837 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2621 0.222011025164073 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1966 0.16652944504867132 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1539 0.13036053709557738 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1387 0.11748542232070557 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1304 0.1104549320160058 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1207 0.10223857587677838 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.470470246626211E-5 0.0 0.0 0.0 0.0 3 8.470470246626211E-5 0.0 0.0 0.0 0.0 4 8.470470246626211E-5 0.0 0.0 0.0 0.0 5 8.470470246626211E-5 0.0 0.0 0.0 0.0 6 8.470470246626211E-5 0.0 0.0 0.0 0.0 7 8.470470246626211E-5 0.0 0.0 0.0 0.0 8 8.470470246626211E-5 0.0 0.0 0.0 0.0 9 8.470470246626211E-5 0.0 0.0 0.0 0.0 10-11 8.470470246626211E-5 0.0 0.0 0.0 0.0 12-13 8.470470246626211E-5 0.0 0.0 0.0 0.0 14-15 8.470470246626211E-5 0.0 0.0 8.470470246626211E-5 0.0 16-17 8.470470246626211E-5 0.0 0.0 8.470470246626211E-5 0.0 18-19 8.470470246626211E-5 0.0 0.0 2.117617561656553E-4 0.0 20-21 8.470470246626211E-5 0.0 0.0 2.964664586319174E-4 0.0 22-23 8.470470246626211E-5 0.0 0.0 5.929329172638348E-4 0.0 24-25 8.470470246626211E-5 0.0 0.0 0.0011435134832945386 0.0 26-27 8.470470246626211E-5 0.0 0.0 0.002498788722754733 0.0 28-29 8.470470246626211E-5 0.0 0.0 0.004531701581945023 0.0 30-31 8.470470246626211E-5 0.0 0.0 0.011901010696509827 0.0 32-33 8.470470246626211E-5 0.0 0.0 0.03172191107361516 0.0 34-35 8.470470246626211E-5 0.0 0.0 0.05899682526775157 0.0 36-37 8.470470246626211E-5 0.0 0.0 0.10549970692172947 0.0 38-39 8.470470246626211E-5 0.0 0.0 0.17597401937365953 0.0 40-41 8.470470246626211E-5 0.0 0.0 0.25263177510562673 0.0 42-43 8.470470246626211E-5 0.0 0.0 0.33132244369678426 0.0 44-45 8.470470246626211E-5 0.0 0.0 0.4298340126650471 0.0 46-47 1.2705705369939316E-4 0.0 0.0 0.5515123177578327 0.0 48-49 1.6940940493252423E-4 0.0 0.0 0.6798822943454529 0.0 50-51 1.6940940493252423E-4 0.0 0.0 0.820492100439448 0.0 52-53 1.6940940493252423E-4 0.0 0.0 0.9559772720342342 0.0 54-55 1.6940940493252423E-4 0.0 0.0 1.112638619245586 0.0 56-57 1.6940940493252423E-4 0.0 0.0 1.3028853809848107 0.0 58-59 1.6940940493252423E-4 0.0 0.0 1.5144353753943003 0.0 60-61 1.6940940493252423E-4 0.0 0.0 1.7423757297310116 0.0 62-63 1.6940940493252423E-4 0.0 0.0 1.9709937216874533 0.0 64-65 1.6940940493252423E-4 0.0 0.0 2.206726908651061 0.0 66-67 2.5411410739878637E-4 0.0 0.0 2.4768078524647374 0.0 68-69 2.5411410739878637E-4 8.470470246626211E-5 0.0 2.7965257519236437 0.0 70-71 2.5411410739878637E-4 8.470470246626211E-5 0.0 3.124290598116845 0.0 72-73 2.5411410739878637E-4 8.470470246626211E-5 0.0 3.4447284875467146 0.0 74-75 2.5411410739878637E-4 8.470470246626211E-5 0.0 3.7830814215481983 0.0 76-77 2.5411410739878637E-4 8.470470246626211E-5 0.0 4.1457446051575 0.0 78-79 3.3881880986504846E-4 8.470470246626211E-5 0.0 4.54072263275768 0.0 80-81 3.3881880986504846E-4 8.470470246626211E-5 0.0 4.942646445960094 0.0 82-83 3.3881880986504846E-4 8.470470246626211E-5 0.0 5.346518467319232 0.0 84-85 3.3881880986504846E-4 8.470470246626211E-5 0.0 5.7597503583008915 0.0 86-87 3.3881880986504846E-4 8.470470246626211E-5 0.0 6.184036212954398 0.0 88-89 3.3881880986504846E-4 8.470470246626211E-5 0.0 6.638900465198226 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1315 0.0 46.63707 2 GTACCTG 1560 0.0 39.357727 1 ACCTGGG 1475 0.0 39.321953 3 CGACGTC 50 0.0016090174 38.03271 4 GGTATCA 3380 0.0 37.738358 1 GTATCAA 5165 0.0 35.661987 1 TACGACG 90 2.3988674E-5 31.693924 4 GTACGAC 125 2.1378219E-7 30.426168 3 TAACGCA 65 0.005819614 29.255932 4 TATCAAC 6405 0.0 28.873386 2 GTACATG 5290 0.0 28.521248 1 TACATGG 5325 0.0 28.390614 2 ATCAACG 6490 0.0 28.348728 3 TGTACGA 135 4.193589E-7 28.172377 2 TCAACGC 6540 0.0 28.131992 4 CAACGCA 6615 0.0 27.741167 5 AACGCAG 6770 0.0 27.169338 6 TAGGACC 1385 0.0 27.117186 4 ACATGGG 5335 0.0 26.822506 3 TAACCGT 90 8.902852E-4 26.411604 5 >>END_MODULE