FastQCFastQC Report
Thu 26 May 2016
SRR938542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938542_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180572
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48020.40675198124299067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41860.35457388452377325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.23497084464141113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18590.15746604188478128No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15640.13247815465723395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14090.11934892577496334No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA13640.11553721416398154No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC13130.11121727433820215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12750.044.012672
TAACGCA951.9163053E-840.0285344
ATAACGC1208.54925E-1139.6115723
GTACCTG15150.037.3542671
ACCTGGG14150.037.2880673
GGTATCA32800.036.681961
TATAACG1203.750756E-935.6670462
GTATCAA50000.033.8599171
GTACGAC856.3806167E-427.9611113
GTACATG50000.027.2020681
ATCAACG61950.027.1622223
TATCAAC62600.027.0446622
TCAACGC62850.026.8488964
TACATGG49700.026.7921492
CAACGCA63950.026.3870685
ACATGGG50100.025.90173
AACGCAG66650.025.4402756
CCTGGGG11400.025.0178364
TAGGACC15800.024.3686374
CATGGGG25550.023.441374
Produced by FastQC (version 0.11.5)