##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938542_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1180572 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.819002144723065 31.0 31.0 34.0 30.0 34.0 2 32.05601691383499 33.0 31.0 34.0 30.0 34.0 3 32.11568121215817 33.0 31.0 34.0 30.0 34.0 4 35.763373178425375 37.0 35.0 37.0 35.0 37.0 5 35.64880159786951 37.0 35.0 37.0 33.0 37.0 6 35.68629698146322 37.0 35.0 37.0 33.0 37.0 7 35.64013630680721 37.0 35.0 37.0 33.0 37.0 8 35.63196992644244 37.0 35.0 37.0 33.0 37.0 9 37.216591618300285 39.0 37.0 39.0 33.0 39.0 10-11 37.11376010950624 39.0 37.0 39.0 33.0 39.0 12-13 37.03046277567145 39.0 37.0 39.0 33.0 39.0 14-15 38.082287230257876 40.0 37.0 41.0 33.0 41.0 16-17 38.030503857452146 40.0 37.0 41.0 33.0 41.0 18-19 38.160436635800274 40.0 37.5 41.0 33.0 41.0 20-21 38.23413269161051 40.0 37.5 41.0 33.0 41.0 22-23 38.12464762843774 40.0 37.0 41.0 33.0 41.0 24-25 38.036342976116664 40.0 37.0 41.0 33.0 41.0 26-27 37.859299136350856 40.0 37.0 41.0 33.0 41.0 28-29 37.747326296066646 40.0 37.0 41.0 32.5 41.0 30-31 37.50379222952942 39.0 36.5 41.0 32.0 41.0 32-33 37.276151306315924 39.0 36.0 41.0 31.5 41.0 34-35 37.11622543987152 39.0 36.0 41.0 31.0 41.0 36-37 37.0595927228496 39.0 36.0 41.0 31.0 41.0 38-39 36.93152641262032 39.0 36.0 41.0 31.0 41.0 40-41 36.73981341248141 39.0 35.0 41.0 30.0 41.0 42-43 36.572319180871645 39.0 35.0 41.0 30.0 41.0 44-45 36.321833399403005 39.0 35.0 41.0 30.0 41.0 46-47 35.92519939486961 39.0 35.0 40.0 29.0 41.0 48-49 35.581978481617384 39.0 35.0 40.0 27.5 41.0 50-51 35.28561705681653 38.0 34.5 40.0 27.0 41.0 52-53 34.873232212859534 38.0 34.0 40.0 26.0 41.0 54-55 34.62366632445967 38.0 34.0 40.0 24.5 41.0 56-57 34.31220374530312 38.0 33.0 40.0 24.0 41.0 58-59 33.92860875914387 38.0 33.0 40.0 23.0 41.0 60-61 33.634810922163155 37.0 33.0 40.0 22.5 41.0 62-63 33.240308087943816 37.0 32.0 39.5 21.5 41.0 64-65 32.774722761508826 36.0 31.0 39.0 20.0 40.0 66-67 32.288906987460315 36.0 31.0 39.0 18.5 40.0 68-69 31.778853386324595 35.0 30.5 38.0 17.0 40.0 70-71 31.250941492767915 35.0 29.5 38.0 13.0 40.0 72-73 30.827813127873608 34.0 29.0 37.5 10.0 39.0 74-75 30.3429350348814 34.0 29.0 37.0 8.0 39.0 76-77 29.005259738499642 32.5 27.5 35.5 7.5 38.0 78-79 29.74243205835815 34.0 29.0 36.0 7.0 39.0 80-81 29.452807198544434 34.0 29.0 36.0 2.0 37.5 82-83 28.9806703868972 34.0 28.5 35.0 2.0 37.0 84-85 28.45376520872933 33.0 27.5 35.0 2.0 36.5 86-87 27.870821517027338 33.0 26.0 35.0 2.0 36.0 88-89 27.19925298922895 32.5 26.0 35.0 2.0 36.0 90-91 26.63120504297917 32.0 25.0 34.5 2.0 35.0 92-93 25.943241072971407 32.0 24.0 34.0 2.0 35.0 94-95 25.178303822214993 31.5 20.5 34.0 2.0 35.0 96-97 24.08508248543926 31.0 12.0 34.0 2.0 35.0 98-99 22.831521076224067 31.0 2.0 34.0 2.0 35.0 100-101 19.287928224623318 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40156430028148193 1101 2 0.3398606665150652 1101 3 0.30675249507129365 1101 4 0.27767251077741406 1101 5 0.0729567648659426 1101 6 0.10659006207765032 1101 7 0.18293176275112444 1101 8 0.26020123265440986 1101 9 0.2398734131009661 1101 10-11 0.19606743044269592 1101 12-13 0.28761816175013877 1101 14-15 0.40465354641730755 1101 16-17 0.4505731161014239 1101 18-19 0.4002321435098892 1101 20-21 0.5104550639608192 1101 22-23 0.4761743829418563 1101 24-25 0.4661212855451353 1101 26-27 0.42181102187909403 1101 28-29 0.4035219993924599 1101 30-31 0.4189411321915202 1101 32-33 0.47514660114835294 1101 34-35 0.35355690313238597 1101 36-37 0.5882842456711472 1101 38-39 0.6210700301148293 1101 40-41 0.5682605036211754 1101 42-43 0.6198614589287317 1101 44-45 0.5067234985037885 1101 46-47 0.5846613837100989 1101 48-49 0.6342312100227048 1101 50-51 0.4241857046967965 1101 52-53 0.6136347105010742 1101 54-55 0.4592671568957627 1101 56-57 0.5767889714585976 1101 58-59 0.745716567502491 1101 60-61 0.6460706730468218 1101 62-63 0.6675283793266757 1101 64-65 0.6461786243816832 1101 66-67 0.7977090412046266 1101 68-69 0.6363672152337116 1101 70-71 0.4544931378879298 1101 72-73 0.33889502954156114 1101 74-75 0.4811699457025398 1101 76-77 0.3655486155431653 1101 78-79 0.3977416016930988 1101 80-81 0.3596181585411493 1101 82-83 0.47081317725057303 1101 84-85 0.5664635252396018 1101 86-87 0.5782897967606786 1101 88-89 0.8266920174520926 1101 90-91 1.1658492115585766 1101 92-93 0.7042332692885118 1101 94-95 0.75186830384866 1101 96-97 0.9617937770738365 1101 98-99 0.7166397004396465 1101 100-101 1.1642547705133097 1102 1 0.30301916956888775 1102 2 0.34779951767800554 1102 3 0.28282232367628524 1102 4 0.22978976445659072 1102 5 0.1242696517769204 1102 6 0.25517107844583364 1102 7 0.20566094487022468 1102 8 0.1775682096899871 1102 9 0.16310371419642422 1102 10-11 0.40556844780303436 1102 12-13 0.4043125527861591 1102 14-15 0.35388154474983935 1102 16-17 0.3605497284155206 1102 18-19 0.3589720409177204 1102 20-21 0.5056935135404785 1102 22-23 0.457891101496692 1102 24-25 0.46858207336529745 1102 26-27 0.5113843386338175 1102 28-29 0.43245335897473325 1102 30-31 0.32850968019467786 1102 32-33 0.33630912036138483 1102 34-35 0.42502336624697534 1102 36-37 0.336305333794634 1102 38-39 0.5343523634105551 1102 40-41 0.5132610357050282 1102 42-43 0.5719619099598177 1102 44-45 0.6984921212387363 1102 46-47 0.4523629334743404 1102 48-49 0.4534819239352146 1102 50-51 0.40728744153051366 1102 52-53 0.37533995524764663 1102 54-55 0.20343403759615342 1102 56-57 0.1731029505417041 1102 58-59 0.35109477510800247 1102 60-61 0.3565774489146989 1102 62-63 0.21622325363886574 1102 64-65 0.24283585756163717 1102 66-67 0.14653161564235972 1102 68-69 0.39409783357915984 1102 70-71 0.29698711493871244 1102 72-73 0.12001733441680784 1102 74-75 0.20905466033356745 1102 76-77 0.42721273380820435 1102 78-79 0.39191468337076785 1102 80-81 0.5244861417352986 1102 82-83 0.2483269134153936 1102 84-85 0.2638822384287991 1102 86-87 0.32671454850155257 1102 88-89 0.3350481343761196 1102 90-91 0.38014858513985317 1102 92-93 0.4239078900087385 1102 94-95 0.32814192457128755 1102 96-97 0.339479300312302 1102 98-99 0.4699836274425415 1102 100-101 0.5859864354237025 1103 1 0.3359761366166616 1103 2 0.35282643601490804 1103 3 0.3718846036744452 1103 4 0.14580977404372675 1103 5 0.20313333883760976 1103 6 0.16409565805940218 1103 7 0.2523436533785741 1103 8 0.20631537982368542 1103 9 0.29073285972313556 1103 10-11 0.34397663515402144 1103 12-13 0.42882382241725026 1103 14-15 0.5488049504929506 1103 16-17 0.6017045669925167 1103 18-19 0.5063766323016381 1103 20-21 0.4707131228725885 1103 22-23 0.478507399311745 1103 24-25 0.4878967513340271 1103 26-27 0.718975103975211 1103 28-29 0.6328703835769645 1103 30-31 0.6588272580347976 1103 32-33 0.60480390834487 1103 34-35 0.6289718899703871 1103 36-37 0.6234788214080851 1103 38-39 0.7378887668423033 1103 40-41 0.6953102306861751 1103 42-43 0.797752018123596 1103 44-45 0.7901679241613877 1103 46-47 0.7735209203023103 1103 48-49 0.7665414701757385 1103 50-51 0.5924583883946895 1103 52-53 0.8418945346871993 1103 54-55 0.905800130142083 1103 56-57 0.8645395496712638 1103 58-59 0.7473286600971747 1103 60-61 0.7191321675733846 1103 62-63 0.7338487130224749 1103 64-65 0.7104401176672823 1103 66-67 0.7263666726035609 1103 68-69 0.5582546006576337 1103 70-71 0.4339617406686216 1103 72-73 0.3509897959219259 1103 74-75 0.4401936310928001 1103 76-77 0.6966500195412486 1103 78-79 0.7617503256851599 1103 80-81 0.6229872420411056 1103 82-83 0.7362884129953606 1103 84-85 0.6407927442530656 1103 86-87 0.6147059485556596 1103 88-89 0.6205244932261031 1103 90-91 0.5171872833143425 1103 92-93 0.5300039480504672 1103 94-95 0.5765915296160848 1103 96-97 0.8107918520250905 1103 98-99 0.813086426538252 1103 100-101 0.45924599843380776 1104 1 0.3633241391726365 1104 2 0.28920967783816565 1104 3 0.2723156279561749 1104 4 0.2308106023676757 1104 5 0.09617600935401072 1104 6 0.02154110701098233 1104 7 0.17161165167458847 1104 8 0.2130617825311205 1104 9 0.4146708300928026 1104 10-11 0.30308257778667524 1104 12-13 0.2944156894594201 1104 14-15 0.44182543376080474 1104 16-17 0.4234529517608223 1104 18-19 0.36322820256717847 1104 20-21 0.36575708320770417 1104 22-23 0.3645603469616745 1104 24-25 0.5015433883388098 1104 26-27 0.33195109441398785 1104 28-29 0.2803652953013014 1104 30-31 0.4231801891182414 1104 32-33 0.3881387701331036 1104 34-35 0.4267972437830423 1104 36-37 0.6286994809906119 1104 38-39 0.47579227893583464 1104 40-41 0.39044042546063906 1104 42-43 0.5607922036681572 1104 44-45 0.4013859449404791 1104 46-47 0.42599243187518 1104 48-49 0.4655011840489891 1104 50-51 0.4279998460081629 1104 52-53 0.5635762097948742 1104 54-55 0.5800595197856637 1104 56-57 0.4088844637622415 1104 58-59 0.4947586371407695 1104 60-61 0.38924845833876276 1104 62-63 0.5055663314727994 1104 64-65 0.4724042216574347 1104 66-67 0.48004309968737857 1104 68-69 0.5844081896669593 1104 70-71 0.41323293392843397 1104 72-73 -0.14189549304479954 1104 74-75 -0.015173760160099192 1104 76-77 0.019978136405921276 1104 78-79 0.02854903113316709 1104 80-81 -0.0694545977847092 1104 82-83 0.15217359546329234 1104 84-85 0.19686361919981366 1104 86-87 0.5702530565217678 1104 88-89 0.43659442151691863 1104 90-91 0.6079323902864644 1104 92-93 0.5733637483060612 1104 94-95 0.3729939412220844 1104 96-97 0.41914623859204525 1104 98-99 0.20132777646962197 1104 100-101 -0.20769825536465092 1105 1 0.26411978806882175 1105 2 0.2925986048584477 1105 3 0.20090624500952714 1105 4 0.192533097135545 1105 5 0.18060030667381 1105 6 0.06312244688540858 1105 7 0.19339248746458537 1105 8 0.3435477795606232 1105 9 0.2866848611930166 1105 10-11 0.30669232304400396 1105 12-13 0.35131471223323985 1105 14-15 0.4997156772515865 1105 16-17 0.390215236005929 1105 18-19 0.3362549736138263 1105 20-21 0.2875163997682222 1105 22-23 0.267267058148299 1105 24-25 0.3760018913880643 1105 26-27 0.3355059867334518 1105 28-29 0.3506136243964235 1105 30-31 0.40417118479751935 1105 32-33 0.5101137712582968 1105 34-35 0.6265807856513064 1105 36-37 0.6444770846767298 1105 38-39 0.5958541080422108 1105 40-41 0.7513154423384663 1105 42-43 0.7543246203479512 1105 44-45 0.7857816202786054 1105 46-47 0.5669381105888576 1105 48-49 0.5300922764944715 1105 50-51 0.32327961005614014 1105 52-53 0.24432296925208163 1105 54-55 0.3103353175442862 1105 56-57 0.3654268721156413 1105 58-59 0.6087827752464037 1105 60-61 0.6379625788914538 1105 62-63 0.7261092489739767 1105 64-65 0.5070533456982176 1105 66-67 0.36815963264415075 1105 68-69 0.40545505095371936 1105 70-71 0.5826764719710553 1105 72-73 0.6885062554980834 1105 74-75 0.5883411176444362 1105 76-77 0.4612689666824963 1105 78-79 0.6375579932593212 1105 80-81 0.6804251476976582 1105 82-83 0.7896779555686102 1105 84-85 0.8209082638652951 1105 86-87 0.7829129739329339 1105 88-89 1.037387046469096 1105 90-91 0.8318276661826758 1105 92-93 0.8914278052856055 1105 94-95 0.9719760676032543 1105 96-97 0.8404537216579584 1105 98-99 0.8009435246074332 1105 100-101 0.1955657757355631 1106 1 0.04059858685683437 1106 2 0.07649169533284095 1106 3 0.12596385605979776 1106 4 0.15698111935768821 1106 5 0.14051407148252792 1106 6 0.16934305833990493 1106 7 0.17982910148955256 1106 8 0.1112793725277541 1106 9 -0.06269964203907819 1106 10-11 0.017729460146213682 1106 12-13 0.08445963641467102 1106 14-15 0.1608417789441603 1106 16-17 -0.08590943188838196 1106 18-19 0.08523006698346336 1106 20-21 0.17411401378973324 1106 22-23 0.3003995445617633 1106 24-25 0.19120498977427047 1106 26-27 0.1282243645721124 1106 28-29 0.314572427744956 1106 30-31 0.25425531247811506 1106 32-33 0.1854700614867042 1106 34-35 0.19053855251916474 1106 36-37 -0.36695944297485283 1106 38-39 0.005329959770193682 1106 40-41 0.18207473352388348 1106 42-43 0.2603324077726725 1106 44-45 0.33686767926817396 1106 46-47 0.025420841093527713 1106 48-49 0.004385828548251425 1106 50-51 0.039903723433312166 1106 52-53 0.18252246414050433 1106 54-55 -0.21143250387699908 1106 56-57 -0.06968736532825659 1106 58-59 0.0613831324867391 1106 60-61 -0.28906158147283634 1106 62-63 -0.293271211983523 1106 64-65 -0.5051215016800086 1106 66-67 -0.2659326348645976 1106 68-69 -0.8366011884871334 1106 70-71 -0.7532286002198205 1106 72-73 -0.49869009885392757 1106 74-75 0.012199866059479092 1106 76-77 -0.14776430780176852 1106 78-79 -0.38651979806948233 1106 80-81 -0.895707121054869 1106 82-83 -0.3109214757489944 1106 84-85 -0.27760404088765966 1106 86-87 -0.11637150891503723 1106 88-89 0.044873200814887326 1106 90-91 -0.020436525828820606 1106 92-93 -0.10111057041099514 1106 94-95 0.15805267734409867 1106 96-97 -0.15274558320901122 1106 98-99 -0.403289807919748 1106 100-101 -0.27207598890732143 1107 1 0.0469063100531919 1107 2 0.14361791672221358 1107 3 0.1075760900311522 1107 4 0.015564307685892231 1107 5 0.10139614884834458 1107 6 -0.013724804061382656 1107 7 0.11468052700153208 1107 8 0.1662385480062767 1107 9 0.22573229478329893 1107 10-11 0.11469439121300695 1107 12-13 0.15880376705315058 1107 14-15 0.2526634895585431 1107 16-17 0.2854816134870646 1107 18-19 0.2371792705706639 1107 20-21 0.20367665600630858 1107 22-23 0.12141246117266036 1107 24-25 0.07932237700489253 1107 26-27 0.14594956869471787 1107 28-29 0.22384829442946597 1107 30-31 0.1287081969648014 1107 32-33 0.03458329933972948 1107 34-35 0.08418063523901509 1107 36-37 0.27865861351022403 1107 38-39 0.12706734432379818 1107 40-41 0.1457777183987332 1107 42-43 0.0690092917239511 1107 44-45 -0.010430205887764998 1107 46-47 -0.006671582946090382 1107 48-49 -0.05389452214107848 1107 50-51 -0.11104494475033277 1107 52-53 -0.10936951819117269 1107 54-55 -0.09076927271651414 1107 56-57 -0.18410812560131262 1107 58-59 -0.34898863836053806 1107 60-61 -0.35266250504398045 1107 62-63 -0.08281913060218216 1107 64-65 -0.1146516850557262 1107 66-67 -0.4561682124311872 1107 68-69 -0.30471288705492583 1107 70-71 -0.3704200739181225 1107 72-73 -0.3721374285286707 1107 74-75 -0.15525930941903354 1107 76-77 -0.16524690718605584 1107 78-79 -0.05377987034896137 1107 80-81 0.13428886341842983 1107 82-83 0.06232507759058237 1107 84-85 -0.17810096231718475 1107 86-87 0.004510903078003281 1107 88-89 -0.23563476331307243 1107 90-91 -0.2014698547004592 1107 92-93 -0.4087417442833008 1107 94-95 -0.6506710544187193 1107 96-97 -0.38140138564509485 1107 98-99 -0.5602349959802488 1107 100-101 -1.2044996938999581 1108 1 0.1693783311196846 1108 2 0.23916762047468154 1108 3 0.24267483920824162 1108 4 0.1900508119650226 1108 5 0.1644178736673041 1108 6 0.11942858632703235 1108 7 0.14925919717928338 1108 8 0.05432281397072103 1108 9 0.12256244619870671 1108 10-11 0.20938497712478465 1108 12-13 0.11428434638165186 1108 14-15 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-0.801800563756526 2316 76-77 -0.6374950115428213 2316 78-79 -0.3018328198413158 2316 80-81 -0.5327076665905679 2316 82-83 -0.607135788621985 2316 84-85 -0.5964613915679244 2316 86-87 -0.5864976433813247 2316 88-89 -0.2998392357459245 2316 90-91 -0.24881595417353353 2316 92-93 -0.411654287658191 2316 94-95 -0.5132819013535581 2316 96-97 -0.12398427987462668 2316 98-99 -0.552136743827571 2316 100-101 -0.3527309623744479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 15.0 5 46.0 6 100.0 7 248.0 8 481.0 9 921.0 10 1587.0 11 2731.0 12 4019.0 13 5657.0 14 6148.0 15 7724.0 16 10157.0 17 12684.0 18 11935.0 19 8840.0 20 8410.0 21 9021.0 22 9840.0 23 11338.0 24 13179.0 25 15760.0 26 18910.0 27 23042.0 28 27622.0 29 32976.0 30 39269.0 31 47281.0 32 57070.0 33 70221.0 34 89683.0 35 118406.0 36 158664.0 37 210285.0 38 137683.0 39 8611.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.84571585397463 20.929353985644124 15.491604845827665 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32.896807649173454 19.009725793937175 20-21 20.847394313942733 27.91672172472327 31.301860454084967 19.93402350724903 22-23 19.982686358815897 29.41239500852129 31.54237945673792 19.062539175924893 24-25 20.547245518466976 28.133303743031952 32.53699281785786 18.782457920643218 26-27 19.084202585668315 29.493501944355145 32.04503127360027 19.37726419637627 28-29 19.030436093690177 29.78509569937285 30.776860708199077 20.4076074987379 30-31 19.711885425031255 28.075881860657375 32.10240459709362 20.109828117217756 32-33 19.049875822906184 29.012715869934237 32.67005316067127 19.267355146488313 34-35 19.561247790545632 29.384927719656723 31.914030252317254 19.139794237480395 36-37 19.196753776982682 29.048630663779928 31.611159675140527 20.143455884096863 38-39 20.054382468853966 29.521929815273257 30.921600725099584 19.502086990773194 40-41 21.178879315821707 27.627400337650393 32.2274421839356 18.9662781625923 42-43 19.459853680725143 29.53762126567782 31.76654647038843 19.235978583208606 44-45 19.154824949261883 29.857433515279038 30.830478784860222 20.15726275059886 46-47 20.63376905432282 28.604100385236986 30.596397339594706 20.16573322084549 48-49 20.023217558946 29.102799321007105 30.427284401120815 20.44669871892608 50-51 19.044708836055744 30.270538349206994 30.51681727162765 20.16793554310961 52-53 19.296705325892873 29.27695218927774 30.900571926151056 20.525770558678335 54-55 19.737550949878532 28.652509122696458 30.786771158387626 20.82316876903738 56-57 20.205713840409565 29.36208041525633 29.023727481254852 21.408478263079253 58-59 20.001914326275738 29.983855283709932 28.7971424021576 21.217087987856733 60-61 19.522866881477793 28.168802919262863 31.76295050196007 20.545379697299275 62-63 20.033932703807984 28.23470317778162 31.066042562418893 20.665321555991504 64-65 20.60577415015772 29.198981510657546 29.271742850076066 20.92350148910867 66-67 20.247270072164202 30.394177735105416 28.788514715118907 20.57003747761148 68-69 20.641567321326622 28.2864855890669 30.83963400482143 20.23231308478504 70-71 21.23501202595523 28.27262050625481 29.943379117656153 20.54898835013381 72-73 20.371692704892205 29.77920872255144 29.0551105735186 20.793987999037753 74-75 19.951006800093516 29.951074563855485 30.022014752170982 20.075903883880017 76-77 20.1010611805125 28.806417567077652 30.816248394845893 20.27627285756396 78-79 19.822001538237398 28.52731557245132 30.465274460176932 21.185408429134352 80-81 19.940731441940404 29.417601923157555 30.010456915095695 20.631209719806346 82-83 19.70504041061573 29.142823986918167 30.69961632891272 20.452519273553378 84-85 19.684628498215815 29.07233087324361 30.428225029383427 20.814815599157146 86-87 18.658347959259736 30.567395591214957 29.040016873462584 21.734239576062723 88-89 19.435290450430152 29.137371179975126 29.913308935545203 21.514029434049526 90-91 19.70718397948729 29.00130576225301 30.653054776366506 20.63845548189319 92-93 19.15138901771144 29.88693120960354 30.122454131053406 20.839225641631607 94-95 19.125347712803624 30.100493659005974 29.576468017198444 21.19769061099196 96-97 20.26784473966857 29.494855036372197 29.20537671569375 21.031923508265486 98-99 20.101865875185926 28.627478883117675 30.344866725621138 20.925788516075258 100-101 19.231567409696318 29.090347729744565 30.500342206997967 21.177742653561154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 972.0 1 650.5 2 965.5 3 1302.0 4 753.0 5 579.5 6 903.5 7 1521.0 8 2033.0 9 2072.0 10 1713.5 11 1425.5 12 1418.0 13 1546.0 14 1645.0 15 1781.5 16 1980.5 17 2141.5 18 2329.5 19 2591.5 20 2968.0 21 3441.0 22 3949.5 23 4586.0 24 5379.0 25 6425.5 26 7812.0 27 9593.0 28 11678.0 29 13799.5 30 16642.5 31 21116.0 32 27591.5 33 36343.5 34 47333.5 35 58118.5 36 66114.5 37 68200.5 38 63106.0 39 54457.5 40 47166.0 41 42845.5 42 39646.0 43 37238.5 44 35775.5 45 34917.5 46 33828.0 47 33218.0 48 33246.0 49 31988.0 50 29963.5 51 27584.5 52 25265.0 53 23770.5 54 22643.0 55 20836.0 56 18135.0 57 15721.5 58 13712.5 59 11520.0 60 9377.0 61 7617.0 62 6068.5 63 4817.0 64 3779.0 65 2867.5 66 2117.0 67 1669.5 68 1287.0 69 874.5 70 631.5 71 470.0 72 395.0 73 337.5 74 260.5 75 203.0 76 133.0 77 64.0 78 36.5 79 25.5 80 12.5 81 3.5 82 3.5 83 4.0 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.470470246626212E-4 2 0.04794286159590436 3 0.0010164564295951455 4 0.0 5 0.0849588165736609 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.235235123313106E-4 26-27 0.00876693670525813 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011308077779245992 36-37 0.0 38-39 0.0037693592597486643 40-41 0.0051669868504419895 42-43 2.5411410739878637E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.235235123313106E-5 68-69 8.470470246626212E-4 70-71 4.235235123313106E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011435134832945386 82-83 2.964664586319174E-4 84-85 0.005039929796742596 86-87 0.0016940940493252424 88-89 4.235235123313106E-5 90-91 0.0030070169375523053 92-93 6.352852684969658E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1180572.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.39347343236976 #Duplication Level Percentage of deduplicated Percentage of total 1 87.79473169582815 63.55765576523966 2 8.125962663297258 11.765333243556775 3 1.7230064489211598 3.7420326475132724 4 0.7461118986186465 2.160545276408958 5 0.3992569167737236 1.4451797498574215 6 0.23217326827088056 1.008469759696467 7 0.18561932544473983 0.9406339393582718 8 0.12439576936958219 0.7204353459964835 9 0.0935521581030352 0.6095309104955641 >10 0.51342636228982 6.750444528031769 >50 0.03799763745110269 1.9076604654566138 >100 0.021424401297905137 2.9644980158404515 >500 0.0012877998836732945 0.6967544628111251 >1k 0.00105365445027815 1.7308258897371118 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4802 0.40675198124299067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4186 0.35457388452377325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2774 0.23497084464141113 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1859 0.15746604188478128 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1564 0.13247815465723395 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1409 0.11934892577496334 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1364 0.11553721416398154 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1313 0.11121727433820215 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.470470246626211E-5 0.0 0.0 0.0 0.0 8 8.470470246626211E-5 0.0 0.0 0.0 0.0 9 8.470470246626211E-5 0.0 0.0 0.0 0.0 10-11 8.470470246626211E-5 0.0 0.0 0.0 0.0 12-13 8.470470246626211E-5 0.0 0.0 0.0 0.0 14-15 8.470470246626211E-5 0.0 0.0 8.470470246626211E-5 0.0 16-17 8.470470246626211E-5 0.0 0.0 8.470470246626211E-5 0.0 18-19 8.470470246626211E-5 0.0 0.0 1.6940940493252423E-4 0.0 20-21 8.470470246626211E-5 0.0 0.0 2.117617561656553E-4 0.0 22-23 8.470470246626211E-5 0.0 0.0 5.082282147975726E-4 0.0 24-25 8.470470246626211E-5 0.0 0.0 0.0011435134832945386 0.0 26-27 8.470470246626211E-5 0.0 0.0 0.0024564363715216015 0.0 28-29 8.470470246626211E-5 0.0 0.0 0.004531701581945023 0.0 30-31 8.470470246626211E-5 0.0 0.0 0.01198571539897609 0.0 32-33 8.470470246626211E-5 0.0 0.0 0.031764263424848296 0.0 34-35 8.470470246626211E-5 0.0 0.0 0.05942034878008287 0.0 36-37 8.470470246626211E-5 0.0 0.0 0.10655851570255774 0.0 38-39 8.470470246626211E-5 0.0 0.0 0.17728694226188663 0.0 40-41 8.470470246626211E-5 0.0 0.0 0.2545376309111177 0.0 42-43 8.470470246626211E-5 0.0 0.0 0.33416005122940406 0.0 44-45 1.6940940493252423E-4 0.0 0.0 0.4329680866562988 0.0 46-47 2.5411410739878637E-4 0.0 0.0 0.5552393246663482 0.0 48-49 2.5411410739878637E-4 0.0 0.0 0.6844139959273978 0.0 50-51 2.5411410739878637E-4 0.0 0.0 0.8254473255337242 0.0 52-53 2.5411410739878637E-4 0.0 0.0 0.9621183629630383 0.0 54-55 2.5411410739878637E-4 0.0 0.0 1.1204738042237152 0.0 56-57 2.5411410739878637E-4 0.0 0.0 1.311313498880204 0.0 58-59 2.5411410739878637E-4 0.0 0.0 1.52362583561189 0.0 60-61 2.5411410739878637E-4 0.0 0.0 1.7533026363491597 0.0 62-63 2.5411410739878637E-4 0.0 0.0 1.9821747424129996 0.0 64-65 2.5411410739878637E-4 0.0 0.0 2.217738519971675 0.0 66-67 2.5411410739878637E-4 0.0 0.0 2.48955591018591 0.0 68-69 2.5411410739878637E-4 0.0 0.0 2.8109255513429083 0.0 70-71 2.5411410739878637E-4 0.0 0.0 3.141189186258864 0.0 72-73 2.964664586319174E-4 0.0 0.0 3.464507035572587 0.0 74-75 3.3881880986504846E-4 0.0 0.0 3.8092551746102736 0.0 76-77 3.3881880986504846E-4 0.0 0.0 4.178906496173042 0.0 78-79 3.3881880986504846E-4 0.0 0.0 4.58049149056559 0.0 80-81 3.3881880986504846E-4 0.0 0.0 4.9902081363949 0.0 82-83 3.3881880986504846E-4 0.0 0.0 5.406997624880143 0.0 84-85 3.3881880986504846E-4 0.0 0.0 5.831368184236116 0.0 86-87 3.3881880986504846E-4 0.0 0.0 6.2683173919083295 0.0 88-89 3.3881880986504846E-4 0.0 0.0 6.736946158302924 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1275 0.0 44.01267 2 TAACGCA 95 1.9163053E-8 40.028534 4 ATAACGC 120 8.54925E-11 39.611572 3 GTACCTG 1515 0.0 37.354267 1 ACCTGGG 1415 0.0 37.288067 3 GGTATCA 3280 0.0 36.68196 1 TATAACG 120 3.750756E-9 35.667046 2 GTATCAA 5000 0.0 33.859917 1 GTACGAC 85 6.3806167E-4 27.961111 3 GTACATG 5000 0.0 27.202068 1 ATCAACG 6195 0.0 27.162222 3 TATCAAC 6260 0.0 27.044662 2 TCAACGC 6285 0.0 26.848896 4 TACATGG 4970 0.0 26.792149 2 CAACGCA 6395 0.0 26.387068 5 ACATGGG 5010 0.0 25.9017 3 AACGCAG 6665 0.0 25.440275 6 CCTGGGG 1140 0.0 25.017836 4 TAGGACC 1580 0.0 24.368637 4 CATGGGG 2555 0.0 23.44137 4 >>END_MODULE