FastQCFastQC Report
Thu 26 May 2016
SRR938541_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938541_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176603
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50230.42690695162259484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.34472120162875663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.2253946318341871No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.16573134693690225No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15710.1335199723271146No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14180.12051643587514226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12810.1088727463723958No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA12670.10768288029182316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12450.046.9496422
GTACGAC802.2543827E-741.5819243
GTACCTG15350.039.13161
ACCTGGG14950.036.8734133
GGTATCA34900.034.695551
GTATCAA53100.033.7566071
TAGGACC14250.033.348914
GTACATG51750.029.2021181
TACATGG50850.028.8777982
GTGTAGC4900.028.2141741
ATCAACG64200.028.054383
TCAACGC64600.027.880674
TATCAAC64450.027.7243542
ACATGGG50750.027.6237433
CAACGCA65300.027.4362475
AACGCAG67800.026.6314646
GTAGCCC3950.024.0618743
TAGTACG1201.7054376E-423.76114
ACGCAGA75950.023.5800067
CGCAGAG76250.023.4872348
Produced by FastQC (version 0.11.5)